公开(公告)号：WO1997012042A2

公开(公告)日：1997-04-03

申请号：PCT/US1996015702

申请日：1996-09-26

Applicant: UNIVERSITY OF WASHINGTON ,  ROSE, Timothy, M. ,  BOSCH, Marnix, L. ,  STRAND, Kurt

Inventor： UNIVERSITY OF WASHINGTON

IPC: C12N15/38

CPC classification number: C07K14/005 ,  A61K38/00 ,  C07K2319/00 ,  C12N2710/16022 ,  C12N2710/16422

Abstract: This invention relates to polynuclelotides encoding Glycoprotein B from the RFHV/KSHV subfamily of gamma herpes viruses, three members of which are characterized in detail. DNA extracts were obtained from Macaque nemestrina and Macaque mulatta monkeys affected with retroperitoneal fibromatosis (RF), and human AIDS patients affected with Kaposi's sarcoma (KS). The extracts were amplified using consensus-degenerate oligonucleotide probes designed from known protein and DNA sequences of gamma herpes viruses. The nucleotide sequences of a 319 base pair fragment are about 76 % identical between RFHV1 and KSHV, and about 60-63 % identical with the closest related gamma herpes viruses outside the RFHV/KSHV subfamily. Protein sequences encoded within these fragments are about 91 % identical between RFHV1 and KSHV, and

Abstract translation: 本发明涉及编码来自伽马疱疹病毒的RFHV / KSHV亚家族的糖蛋白B的多核苷酸，其中三个成员的细节特征。 从腹膜后纤维瘤（RF）影响的猕猴和猕猴猕猴获得DNA提取物，以及受卡波西肉瘤（KS）影响的人类艾滋病患者。 使用由已知蛋白质和γ疱疹病毒DNA序列设计的共有简并寡核苷酸探针扩增提取物。 319碱基对片段的核苷酸序列在RFHV1和KSHV之间约为76％，与RFHV / KSHV亚科以外最接近的相关γ疱疹病毒约60-63％相同。 在这些片段内编码的蛋白质序列在RFHV1和KSHV之间约为91％，与其他γ疱疹病毒相似。 全长KSHV糖蛋白B序列包括在N末端附近的跨膜结构域和细胞外结构域中的多个潜在的抗原位点。 提供材料和方法来表征RFHV / KSHV亚科成员的糖蛋白B编码区，包括但不限于RFHV1，RFHV2和KSHV。本发明的肽，多核苷酸和抗体可用于诊断感染，并用于引发 对糖蛋白B的免疫反应。

公开(公告)号：WO1996034602A1

公开(公告)日：1996-11-07

申请号：PCT/US1996005881

申请日：1996-04-26

Applicant: UNIVERSITY OF WASHINGTON ,  LAI, Henry, Chiu-Yuen ,  SINGH, Narendra, P.

Inventor： UNIVERSITY OF WASHINGTON

IPC: A61K31/335

CPC classification number: A61K31/715 ,  A61K31/335 ,  A61K31/55 ,  A61K33/26 ,  A61K38/40 ,  A61K2300/00

Abstract: Methods of inhibiting or killing cancer cells are disclosed wherein compounds having an endoperoxide moiety that is reactive with heme are administered under conditions which enhance intracellular iron concentrations. Representative endoperoxide compounds include endoperoxide bearing sesquiterpene compounds such as artemisinin and its analogs, arteflene and its analogs, 1,2,4-trioxanes and 1,2,4,5-tetraoxanes. Intracellular iron concentrations may be enhanced by the administration of iron salts or complexes. The in vitro course of such treatment is demonstrated by the time response curve of molt-4 human leukemia cells incubated in halotransferin and dihydroartemisinin.

Abstract translation: 公开了抑制或杀死癌细胞的方法，其中具有与血红素反应的内过氧化物部分的化合物在增强细胞内铁浓度的条件下给药。 代表性的内过氧化物化合物包括含有内过氧化物的倍半萜化合物如青蒿素及其类似物，芳香烯及其类似物，1,2,4-三恶烷和1,2,4,5-四氧杂环己烷。 通过施用铁盐或络合物可增强细胞内铁的浓度。 这种治疗的体外过程通过在转铁蛋白和二氢青蒿素中孵育的蜕皮-4人白血病细胞的时间响应曲线来证明。

公开(公告)号：WO1996032475A2

公开(公告)日：1996-10-17

申请号：PCT/US1996004783

申请日：1996-04-10

Applicant: UNIVERSITY OF WASHINGTON ,  CHENG, Cheng

Inventor： UNIVERSITY OF WASHINGTON ,  YOUNG, Elton, T.

IPC: C12N15/11

CPC classification number: C12Q1/6897 ,  C07K14/00 ,  C07K14/395 ,  C07K2319/00

Abstract: Methods for preparing DNA-binding proteins having altered binding specificity are disclosed. The binding specificity of a parent DNA-binding protein comprising first and second Cys2-His2 zinc fingers is altered by the addition of an additional zinc finger, wherein the altered specificity is a result of interactions between nucleotides in a target sequence and amino acid residues in each of the first, second and additional zinc fingers. The altered DNA-binding proteins are useful within methods for preparing polypeptides.

Abstract translation: 公开了具有改变的结合特异性的制备DNA结合蛋白的方法。 包含第一和第二Cys2-His2锌指的亲本DNA结合蛋白的结合特异性通过加入额外的锌指改变，其中改变的特异性是靶序列中的核苷酸与靶序列中的氨基酸残基之间的相互作用的结果 每个第一，第二和另外的锌指。 改变的DNA结合蛋白在制备多肽的方法中是有用的。

公开(公告)号：WO1996030514A1

公开(公告)日：1996-10-03

申请号：PCT/US1996001689

申请日：1996-03-28

Applicant: UNIVERSITY OF WASHINGTON ,  CHEEVER, Martin, A. ,  DISIS, Mary, L.

Inventor： UNIVERSITY OF WASHINGTON

IPC: C12N15/12

CPC classification number: A61K39/0011 ,  A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/4705 ,  C07K14/71 ,  G01N33/57407 ,  G01N33/57488 ,  G01N2333/71 ,  G01N2333/82 ,  Y10S530/806 ,  Y10S530/828

Abstract: Compounds and compositions for eliciting or enhancing immune reactivity to HER-2/neu protein are disclosed. The compounds include polypeptides and nucleic acid molecules encoding such peptides. The compounds may be used for the prevention or treatment of malignancies in which the HER-2/neu oncogene is associated.

Abstract translation: 公开了引发或增强对HER-2 / neu蛋白的免疫反应性的化合物和组合物。 化合物包括编码这些肽的多肽和核酸分子。 该化合物可用于预防或治疗HER-2 / neu致癌基因相关的恶性肿瘤。

公开(公告)号：WO1996022385A1

公开(公告)日：1996-07-25

申请号：PCT/US1996000787

申请日：1996-01-19

Applicant: UNIVERSITY OF WASHINGTON

Inventor： UNIVERSITY OF WASHINGTON ,  LOEB, Lawrence, A. ,  KIM, Baek

IPC: C12Q01/18

CPC classification number: G01N33/502 ,  C12N9/1276 ,  C12Q1/18 ,  C12Q1/48 ,  G01N33/5008

Abstract: Methods and compositions related to hepatitis are disclosed. Using the methods of the present invention, candidate compounds may be screened for the ability to inhibit reverse transcriptase of hepatitis virus ("HV RT"). Active HV RT mutants may be detected by the disclosed methods. The present invention also discloses methods for screening for compounds that inhibit HV RT obtained from an individual patient.

Abstract translation: 公开了与肝炎相关的方法和组合物。 使用本发明的方法，可以筛选候选化合物抑制肝炎病毒逆转录酶（“HV RT”）的能力。 活性HV RT突变体可以通过所公开的方法检测。 本发明还公开了筛选从个体患者获得的抑制HV RT的化合物的方法。

公开(公告)号：WO1996017626A3

公开(公告)日：1996-06-13

申请号：PCT/US1995015888

申请日：1995-12-06

Applicant: RYAN PHARMACEUTICALS, INC. ,  UNIVERSITY OF WASHINGTON ,  NATIONAL RESEARCH COUNCIL OF CANADA ,  MORGAN, A., Charles, Jr. ,  GRAVES, Scott, S. ,  WOODHOUSE, Clive, S. ,  SIKORSKA, Marianna ,  WALKER, Roy ,  WILBUR, D., Scott ,  BOROWY-BOROWSKI, Henryk

Inventor： RYAN PHARMACEUTICALS, INC. ,  UNIVERSITY OF WASHINGTON ,  NATIONAL RESEARCH COUNCIL OF CANADA

IPC: A61K47/48

公开(公告)号：WO1996016668A1

公开(公告)日：1996-06-06

申请号：PCT/US1995015923

申请日：1995-12-04

Applicant: THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE ,  UNIVERSITY OF WASHINGTON

Inventor： THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE ,  UNIVERSITY OF WASHINGTON ,  BEACHY, Philip, A. ,  MOON, Randall, T. ,  PORTER, Jeffrey, A.

IPC: A61K38/17

CPC classification number: G01N33/5058 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2227/50 ,  A01K2227/706 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/43581 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C12N15/62 ,  G01N33/5008 ,  G01N33/5017 ,  G01N33/502 ,  G01N33/5091 ,  G01N33/92 ,  G01N2500/10

Abstract: The present invention provides two novel polypeptides, referred to as the "N" and "C" fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G CF site recognized by the autoproteolytic domain in the native protein. Also provided are methods of use of the N and C fragments.

Abstract translation: 本发明提供两种新的多肽，分别被称为刺猬蛋白的“N”和“C”片段，或分别在识别的G

公开(公告)号：WO1996015758A2

公开(公告)日：1996-05-30

申请号：PCT/US1995014425

申请日：1995-11-07

Applicant: UNIVERSITY OF WASHINGTON

Inventor： UNIVERSITY OF WASHINGTON ,  KAUSHANSKY, Kenneth

IPC: A61K00/00

CPC classification number: A61K38/196 ,  A61K38/1816 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N5/0642 ,  C12N2501/145 ,  C12N2501/22 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5011 ,  A61K2300/00

Abstract: Methods for stimulating erythropoiesis using the hematopoietic protein thrombopoietin, optionally in combination with erythropoietin, are provided. The methods provided may be used to stimulate erythropoiesis in bone marrow and peripheral blood cells and in vitro and in vivo. In addition, methods for treatment of thrombocytopenia and anemia in patients are disclosed.

Abstract translation: 提供了使用造血蛋白血小板生成素刺激红细胞生成的方法，任选地与促红细胞生成素组合。 所提供的方法可用于刺激骨髓和外周血细胞中的体外和体内的红细胞生成。 另外，公开了治疗患者血小板减少症和贫血的方法。

公开(公告)号：WO1996014853A1

公开(公告)日：1996-05-23

申请号：PCT/US1995014773

申请日：1995-11-09

Applicant: UNIVERSITY OF WASHINGTON ,  FRED HUTCHINSON CANCER RESEARCH CENTER

Inventor： UNIVERSITY OF WASHINGTON ,  FRED HUTCHINSON CANCER RESEARCH CENTER ,  ALLEN, Margaret, D. ,  ANDREWS, Robert, G.

IPC: A61K35/12

CPC classification number: A61K39/001 ,  A61K35/12 ,  A61K35/14 ,  A61K35/28 ,  A61K35/39 ,  A61K35/407 ,  A61K48/00

Abstract: Engraftment of grafts in transplant recipients are enhanced by implanting CD34-positive hematopoietic cells obtained from the graft donor in the thymus of the transplant recipient. The methods enhance engraftment of both allogeneic and xenogeneic transplants. Methods are also provided for supplying a gene product of interest in a host mammal by implanting in the thymus of the host CD34-positive hematopoietic progenitor cells which encode the gene product of interest. The CD34-positive hematopoietic cells can be genetically engineered to produce the gene product of interest in the recipient host.

Abstract translation: 通过将移植供体获得的CD34阳性造血细胞植入移植受体的胸腺中来增强移植受体中移植物的移植。 该方法增强了同种异体移植和异种移植物的移植。 还提供了通过植入编码感兴趣的基因产物的宿主CD34阳性造血祖细胞的胸腺中来提供宿主哺乳动物感兴趣的基因产物的方法。 CD34阳性造血细胞可以被遗传工程化以在受体宿主中产生感兴趣的基因产物。

公开(公告)号：WO1996000233A1

公开(公告)日：1996-01-04

申请号：PCT/US1995006994

申请日：1995-06-07

Applicant: CHILDREN'S HOSPITAL AND MEDICAL CENTER ,  UNIVERSITY OF WASHINGTON ,  WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION ,  TARR, Phillip, I. ,  BILGE, Sima, S. ,  BESSER, Thomas, E. ,  VARY, James, C., Jr.

Inventor： CHILDREN'S HOSPITAL AND MEDICAL CENTER ,  UNIVERSITY OF WASHINGTON ,  WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION

IPC: C07H21/04

CPC classification number: C12R1/19 ,  A61K38/00 ,  A61K39/00 ,  C07K14/245 ,  Y02A50/472 ,  Y10S530/825

Abstract: A continuous segment of chromosomal DNA from E. coli O157:H7, isolated on plasmid pSC(overlap) (ATCC No. 69648), which encodes an adhesin (SEQ ID NO:4) that mediates bacterial colonization of bovine intestines.

Abstract translation: 来自大肠杆菌O157：H7的连续染色体DNA片段，分离于质粒pSC（重叠）（ATCC No.69648）上，其编码粘附素（SEQ ID NO：4），其介导牛肠细菌定植。