公开(公告)号：WO1997043901A1

公开(公告)日：1997-11-27

申请号：PCT/US1997008558

申请日：1997-05-20

Applicant: VANDERBILT UNIVERSITY ,  MILLER, Geraldine, G. ,  PEEK, Richard, M., Jr. ,  THOMPSON, Stuart, A. ,  BLASER, Martin, J.

Inventor： VANDERBILT UNIVERSITY

IPC: A01N43/04

CPC classification number: C07K14/205 ,  A61K38/00 ,  A61K39/00

Abstract: A purified IceA protein of Helicobacter pylori is provided. The protein is expressed as either an IceA 1 or an IceA 2 variant. A purified polypeptide fragment of the IceA protein is also provided. An antigenic fragment of IceA is provided. An isolated nucleic acid that encodes an IceA protein of H. pylori is provided. A nucleic acid that encodes an IceA 1 variant and a nucleic acid that encodes an IceA 2 variant is also provided. Fragments of the IceA gene are provided. A method of detecting the presence of an antibody against H. pylori in a sample is provided. The method comprises the following steps: a) contacting the sample with a purified IceA protein of H. pylori or a H. pylori-specific fragment thereof; and b) detecting the binding of the antibody in the sample to the protein or fragment, the detection of biding indicating the presence in the sample of antibodies against H. pylori. A method of detecting the presence of an antibody against an ulcerative Helicobacter pylori strain in a sample is also provided.

Abstract translation: 提供幽门螺杆菌纯化的IceA蛋白。 蛋白质表达为IceA 1或IceA 2变体。 还提供了IceA蛋白的纯化多肽片段。 提供了IceA的抗原片段。 提供编码幽门螺杆菌的IceA蛋白的分离的核酸。 还提供了编码IceA 1变体的核酸和编码IceA 2变体的核酸。 提供了IceA基因的片段。 提供了检测样品中幽门螺杆菌抗体的存在的方法。 该方法包括以下步骤：a）将样品与幽门螺杆菌的纯化IceA蛋白或幽门螺杆菌特异性片段接触; 和b）检测样品中的抗体与蛋白质或片段的结合，检测指示在幽门螺杆菌抗体样品中的存在。 还提供了检测样品中针对溃疡性幽门螺杆菌菌株的抗体存在的方法。

公开(公告)号：WO1996041208A1

公开(公告)日：1996-12-19

申请号：PCT/US1996010027

申请日：1996-06-07

Applicant: VANDERBILT UNIVERSITY ,  UNIVERSITY OF ALASKA

Inventor： VANDERBILT UNIVERSITY ,  UNIVERSITY OF ALASKA

IPC: G01R33/00

CPC classification number: G01V3/02 ,  G01N27/72 ,  G01N33/02 ,  G01N33/12

Abstract: Inclusions such as encysted parasites (21) and spoilage in food products such as fish fillets (1) are detected by immersing the food products in a bath of an electrolyte (5), such as a saline solution, having substantially the same electrical conductivity as the uncontaminated food product. An electrical current (I) passed through the electrolyte (5) also passes through the food product (1). Perturbations in the resulting magnetic field produced by the discontinuities in conductivity at the boundaries of the inclusions or of the spoiled article which have substantially different conductivities from that of the electrolyte and unspoiled food products, provide an indication of the presence of the contamination. Perturbations in the magnetic field at the edges of the container (43) are cancelled by directing the current (I) in a first direction and back over the container (43) in the opposite direction through a cancelling conductor (25).

Abstract translation: 通过将食品浸入诸如盐水溶液的电解液（5）的浴中来检测诸如鱼片（1）的食品中的包裹寄生虫（21）和腐败物，其具有与 未受污染的食品。 通过电解质（5）的电流（I）也通过食品（1）。 由于与电解质和未受污染的食品具有实质上不同电导率的夹杂物或损坏制品的边界处的导电性不均匀性产生的所得磁场的扰动提供了污染物的存在的指示。 在容器（43）的边缘处的磁场中的扰动通过在第一方向上引导电流（I）并且通过消除导体（25）沿相反方向返回到容器（43）上来消除。

公开(公告)号：WO1996021356A1

公开(公告)日：1996-07-18

申请号：PCT/US1995008374

申请日：1995-07-03

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  MITCHELL, William, M.

IPC: A01N43/04

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K47/543 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/541 ,  A61K2039/545 ,  A61K2039/55516 ,  A61K2039/57 ,  C12N2740/16122 ,  C12N2740/16134

Abstract: The invention provides a method of inducing a mucosal immune response in a subject, comprising administering to the mucosa of the subject an amount of antigen-encoding DNA effective to induce a mucosal immune response complexed to a transfection-facilitating lipospermine or a lipospermidine. In the method of inducing a mucosal immune response, the antigen-encoding DNA can encode an antigen that is expressed on the surface of infected cells during the course of infection. DNA encoding the envelope glycoproteins of viral pathogens is used in the present method. Lipospermines and lipospermidines are bifunctional molecules consisting of one or more hydrophobic chains covalently linked to a cationic grouping in which there is coordination of three or more amide hydrogens with a phosphate oxygen of the DNA chain forming an ionic charge complex. One preferred example of a lipospermine is DOGS (droctadecylamidoglycylspermine). The invention also provides a composition, comprising an amount of DNA encoding an envelope antigen or envelope-associated antigen of a pathogen complexed to a lipospermine. More specifically, the invention provides a composition, comprising an amount of DNA encoding an envelope antigen of HIV complexed to a lipospermine.

Abstract translation: 本发明提供了一种在受试者中诱导粘膜免疫应答的方法，包括向受试者的粘膜施用有效诱导与易转移的脂维生素或脂亚精胺复合的粘膜免疫应答的量的抗原编码DNA。 在诱导粘膜免疫应答的方法中，抗原编码DNA可以编码在感染过程中在受感染细胞表面表达的抗原。 在本方法中使用编码病毒病原体的包膜糖蛋白的DNA。 脂质体和脂蛋白是由一个或多个与阳离子基团共价连接的疏水链组成的双功能分子，其中三个或更多个酰胺氢与DNA链的磷酸氧合物形成离子电荷复合物。 脂肪精胺的一个优选实例是DOGS（十八烷基酰氨基甘氨酰精胺）。 本发明还提供一种组合物，其包含一定量的编码与脂质精胺复合的病原体的包膜抗原或包膜相关抗原的DNA。 更具体地，本发明提供一种组合物，其包含一定量的编码与脂质精胺复合的HIV的包膜抗原的DNA。

公开(公告)号：WO1996018363A1

公开(公告)日：1996-06-20

申请号：PCT/US1995016070

申请日：1995-12-07

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  SCHWARTZ, Herbert, S. ,  DEBOER, David, K. ,  DAWSON, John, M.

IPC: A61F02/44

CPC classification number: A61B17/70 ,  A61B17/7007 ,  A61B17/7059 ,  A61B17/8061 ,  A61B17/86 ,  A61F2/44 ,  A61F2/4455 ,  A61F2002/30235 ,  A61F2002/30438 ,  A61F2002/30578 ,  A61F2002/30616 ,  A61F2002/30772 ,  A61F2002/30777 ,  A61F2002/30785 ,  A61F2002/449 ,  A61F2220/0041 ,  A61F2230/0069 ,  A61F2310/00017

Abstract: This invention is a system and implant designed for use in short segment anterior fusion of patient's spine after the removal of a diseased or injured anterior vertebral body. The system includes the placement of the cylinder (20) of the implant in an intra-osseous location for added safety, and for the placement of the cylinder (20) of the implant in the middle column area of the adjacent healthy vertebral bodies for increased biomechanical strength. The system further includes transverse fixation of the cylinder of the implant, thus adding medial-lateral and rotational support to the system. Finally, the system utilizes the concept of modularity, wherein intraoperative exchangeable parts are available to better fit the patient.

Abstract translation: 本发明是设计用于在去除患病或受伤的前椎体后患者脊柱的短段前融合中使用的系统和植入物。 该系统包括将植入物的圆柱体（20）放置在骨内位置以增加安全性，以及用于将植入物圆柱体（20）放置在相邻健康椎体的中间柱区域中以增加 生物力学强度。 该系统还包括植入物圆柱体的横向固定，从而为系统增加内侧和外侧和旋转支撑。 最后，系统利用模块化的概念，其中术中可交换的部件可用于更好地适合患者。

公开(公告)号：WO1996017055A1

公开(公告)日：1996-06-06

申请号：PCT/US1995015714

申请日：1995-12-01

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  HAWIGER, Jack, J. ,  DONAHUE, John, P. ,  PATEL, Hareshkumar ,  ANDERSON, Wayne, F.

IPC: C12N09/36

CPC classification number: C12N15/62 ,  C07K14/47 ,  C07K14/75 ,  C07K16/065 ,  C07K2319/00 ,  C12N9/2462 ,  C30B7/00 ,  C30B29/58

Abstract: The invention relates to methods of crystallizing a peptide or polypeptide comprising linking a peptide or polypeptide to a terminus of a crystallizable carrier protein to form a chimeric protein and crystallizing the chimeric protein. The invention also includes methods for determining the three-dimensional structure of a peptide or polypeptide, the crystallized chimeric proteins themselves, and methods for designing a peptide or polypeptide for screening for improved binding to a molecule by using the three dimensional structural information.

Abstract translation: 本发明涉及使肽或多肽结晶的方法，包括将肽或多肽与可结晶载体蛋白的末端连接以形成嵌合蛋白并使嵌合蛋白结晶。 本发明还包括用于确定肽或多肽的三维结构，结晶的嵌合蛋白本身的方法，以及用于设计肽或多肽以筛选通过使用三维结构信息改善与分子的结合的方法。

公开(公告)号：WO1995034295A1

公开(公告)日：1995-12-21

申请号：PCT/US1995007539

申请日：1995-06-13

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  LIN, Yao-Zhong ,  HAWIGER, Jack, J.

IPC: A61K31/00

CPC classification number: A61K39/00 ,  A61K38/00 ,  A61K47/62 ,  A61K47/65 ,  C07K14/475 ,  C07K14/50 ,  C07K14/501 ,  C07K2319/09 ,  C07K2319/10 ,  C07K2319/40 ,  C12N15/625 ,  C12N15/87

Abstract: The present invention relates to the delivery of biologically active molecules, such as peptides, nucleic acids and therapeutic agents, into the interior of cells by administering to the cells a complex comprising the molecule linked to an importation competent signal peptide. Such delivery can be utilized for purposes such as peptide therapy, gene transfer, and antisense therapy.

Abstract translation: 本发明涉及将生物活性分子例如肽，核酸和治疗剂递送到细胞内部，通过向细胞施用包含与进口的信号肽连接的分子的复合物。 这种递送可用于诸如肽治疗，基因转移和反义治疗的目的。

公开(公告)号：WO1995007389A1

公开(公告)日：1995-03-16

申请号：PCT/US1994010026

申请日：1994-09-08

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  CARNEY, John, F., III

IPC: E01F13/00

CPC classification number: E01F15/148 ,  E01F15/146

Abstract: A crash impact attenuator (10) including one or more cylinders (12), each bolted or otherwise connected to the adjacent cylinder (12) and such cylinders (12) being connected to the platform (14) of a service vehicle (16) or to an abutment adjacent a highway wherein the cylinders (12) are constructed from a high molecular weight/high density polyethylene material (18).

Abstract translation: 包括一个或多个气缸（12）的碰撞冲击衰减器（10），每个汽缸螺栓连接或以其他方式连接到相邻气缸（12），并且所述气缸（12）连接到维修车辆（16）的平台（14）或 连接到邻近高速公路的邻接处，其中气缸（12）由高分子量/高密度聚乙烯材料（18）构成。

公开(公告)号：WO1992020820A1

公开(公告)日：1992-11-26

申请号：PCT/US1992002369

申请日：1992-03-25

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  MATRISIAN, Lynn, M.

IPC: C12Q01/68

CPC classification number: G01N33/57419 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N33/57446 ,  G01N2333/96486

Abstract: This invention relates to a method to determine the metastatic potential of tumor cells. In particular, this invention relates to the detection of the expression of metalloproteinase pump protein. The expression of this protein can be determined by molecular diagnostic means such as a Northern Blot analysis or polymerase chain reaction amplification. Additionally, immunological methods can be employed to detect metalloproteinase pump protein.

Abstract translation: 本发明涉及确定肿瘤细胞转移潜能的方法。 特别地，本发明涉及检测金属蛋白酶泵浦蛋白的表达。 该蛋白质的表达可以通过分子诊断方法如Northern印迹分析或聚合酶链反应扩增来确定。 此外，免疫学方法可用于检测金属蛋白酶泵浦蛋白。

公开(公告)号：WO1992020343A1

公开(公告)日：1992-11-26

申请号：PCT/US1992002576

申请日：1992-04-02

Applicant: GUNDERSON MEDICAL FOUNDATION, LTD. ,  VANDERBILT UNIVERSITY ,  KEIMOWITZ, Rudolph, M. ,  FITZGERALD, Desmond, J.

Inventor： GUNDERSON MEDICAL FOUNDATION, LTD. ,  VANDERBILT UNIVERSITY

IPC: A61K31/60

CPC classification number: A61K31/60

Abstract: A method is disclosed for inducing thromboxane suppression in a mammalian subject by percutaneously administering a pharmaceutical composition containing aspirin. Articles useful for practicing the therapeutic methods of the invention are also disclosed.

Abstract translation: 公开了一种通过经皮施用含有阿司匹林的药物组合物在哺乳动物受试者中诱导凝血恶烷抑制的方法。 还公开了用于实施本发明的治疗方法的制品。

公开(公告)号：WO1992015894A1

公开(公告)日：1992-09-17

申请号：PCT/US1992001786

申请日：1992-03-05

Applicant: VANDERBILT UNIVERSITY

Inventor： VANDERBILT UNIVERSITY ,  WIKSWO, John, P., Jr. ,  CRUM, Duane ,  HENRY, William, P. ,  SEPULVEDA, Nester, G.

IPC: G01R33/02

CPC classification number: G01N27/83

Abstract: Flaws in an electrically conductive sample object (1) are detected by cancelling the magnetic field generated by a detection current passed through the sample object by passing the current back through an unflawed field cancelling object (39) placed next to the sample object, and measuring the uncancelled field produced by any flaw, preferably with a superconducting quantum interference device (SQUID) magnetometer (101). Elongated objects such as tubes (85) and rods (93) are fed through a sleeve (87, 95) which forms the field cancelling object, with the current applied to the elongated member and passed to the field cancelling sleeve through sliding contacts (89, 96).