公开(公告)号：WO1995011451A1

公开(公告)日：1995-04-27

申请号：PCT/US1994011802

申请日：1994-10-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHIEH, Chia-Hui

IPC: G01N27/447

CPC classification number: G01N27/44752

Abstract: Methods for controlling and varying electroosmotic flow in coated capillary electrophoresis columns under electrophoretic conditions are disclosed. The methods described herein involve varying the concentration of a multi-valent buffer compound in electrophoresis buffer compositions in order to control the electroosmotic flow in capillary columns having interior surfaces coated with charged organic coatings. Increasing the concentration of a multi-valent buffer compound which has a charge opposite the charge of the organic coating, in the electrophoresis buffer results in a decrease in electroosmotic flow. Decreasing the concentration of a multi-valent buffer compound results in an increase in electroosmotic flow. The ability to control electroosmotic flow provides enhanced resolution and optimizes separations of electrophoresis sample components.

Abstract translation: 公开了在电泳条件下在涂布的毛细管电泳柱中控制和改变电渗流的方法。 本文描述的方法涉及改变电泳缓冲液组合物中多价缓冲剂化合物的浓度，以便控制具有涂覆带电有机涂层的内表面的毛细管柱中的电渗流。 在电泳缓冲液中增加具有与有机涂层电荷相反的电荷的多价缓冲化合物的浓度导致电渗流减少。 减少多价缓冲剂化合物的浓度导致电渗流量的增加。 控制电渗流量的能力提高了分辨率，优化了电泳样品组分的分离。

公开(公告)号：WO1995009360A1

公开(公告)日：1995-04-06

申请号：PCT/US1994010981

申请日：1994-09-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHIEH, Chia-Hui

IPC: G01N27/447

CPC classification number: G01N27/44752

Abstract: Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface coated with at least one cross-linked organic multivalent ionic compound capable of ionic interaction with charged chemical functionalities on the tubing interior surface. Exemplary columns are prepared by cross-linking a multi-functional amine compound with a diepoxide, alkylating the cross-linked multi-functional amine compound to form a cross-linked quaternary ammonium compound, and then contacting the interior surface of capillary tubing with the cross-linked quaternary ammonium compound.

Abstract translation: 公开了涂布毛细管电泳柱及其用于电泳分离的方法。 涂覆的毛细管柱包括长度的管，其具有涂覆有至少一种能够在管内表面上与带电化学官能团进行离子相互作用的交联的有机多价离子化合物的内表面。 通过将多官能胺化合物与二环氧化物交联，烷基化交联的多官能胺化合物以形成交联的季铵化合物，然后使毛细管内表面与十字 连接的季铵化合物。

公开(公告)号：WO1994016330A1

公开(公告)日：1994-07-21

申请号：PCT/US1993012140

申请日：1993-12-13

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OH, Chan, S. ,  CHENG, Anthony, K. ,  MICHAEL, Josephine, M. ,  DOBASHI, Thomas, S.

IPC: G01N33/531

CPC classification number: G01N33/532 ,  C07H15/234 ,  G01N33/531 ,  Y10S436/816 ,  Y10S436/817

Abstract: A method for making a preconjugate comprising an immunogenic species of a polymorphic analyte is disclosed. The method is carried out by reacting an activated binding moiety, and a polymorphic analyte at room temperature for between about 10 hours and about 60 hours. The attaching reaction results in an excess of the preconjugate comprising the immunogenic species of the polymorphic analyte. The preconjugate can be used to make an immunoreactive conjugate useful as a developer antigen in a competitive inhibition immunoassay for the polymorphic analyte.

Abstract translation: 公开了一种制备包含多态性分析物的免疫原性物质的预缀合物的方法。 该方法通过使活化的结合部分和多晶型分析物在室温下反应约10小时至约60小时来进行。 附着反应导致包含多态性分析物的免疫原性物质的预结合物过量。 预缀合物可用于在多态性分析物的竞争性抑制免疫测定中使免疫反​​应性缀合物用作显影剂抗原。

公开(公告)号：WO1994014019A1

公开(公告)日：1994-06-23

申请号：PCT/US1993010563

申请日：1993-11-01

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WRIGHT, Herschel, E.

IPC: F25D31/00

CPC classification number: F28D1/06 ,  B04B15/02 ,  B21D53/02 ,  F25D31/006

Abstract: A refrigerant tubing (34) is preformed to provide a flat contact surface (42) against the outside surface of a centrifuge chamber (32). The tubing (34) is tightly wound around the centrifuge chamber (32) in a continuous fashion including a flat spiral (46) at the base (33) of the centrifuge chamber. For the section of the tubing in contact with the vertical cylindrical wall (35) of the centrifuge chamber (32), the pressure for maintaining contact pressure between the flat surface (42) of the tubing (34) and the chamber wall (35) is provided by the tension in the wrapping of the tubing (34). For the section of the tubing (34) at the base (33) of the centrifuge chamber (32), contact pressure may be provided by a clamping mechanism (58). To further enhance heat transfer between the refrigerant coils and the centrifuge chamber (32), a high heat conductive epoxy (44) may be applied between the tubing (34) and the centrifuge chamber surface.

Abstract translation: 预制制冷剂管道（34）以提供抵靠离心机室（32）的外表面的平坦接触表面（42）。 管道（34）以连续的方式紧密地卷绕在离心机室（32）周围，包括在离心机室的底部（33）处的扁平螺旋（46）。 对于与离心机室（32）的垂直圆柱形壁（35）接触的管道的部分，用于维持管道（34）的平坦表面（42）和室壁（35）之间的接触压力的压力， 由管（34）的包裹中的张力提供。 对于离心机室（32）的底座（33）处的管道（34）的部分，接触压力可由夹紧机构（58）提供。 为了进一步增强制冷剂盘管与离心机室（32）之间的热传递，可以在管道（34）和离心机腔室表面之间施加高导热环氧树脂（44）。

公开(公告)号：WO1993005152A1

公开(公告)日：1993-03-18

申请号：PCT/US1992005122

申请日：1992-06-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  PANG, Patty, P., Y.

IPC: C12N15/13

CPC classification number: C07K16/1267 ,  A61K38/00 ,  C07K14/315

Abstract: Disclosed are antibodies to derivatives and variants of Streptolysin O ("SLO"). Methodologies for generating such antibodies are further disclosed, as are purification protocols for wild-type SLO and assaying techniques for wild-type SLO.

Abstract translation: 公开了针对链球菌溶血素O（“SLO”）的衍生物和变体的抗体。 进一步公开了产生这种抗体的方法，野生型SLO的纯化方案和用于野生型SLO的测定技术也是如此。

公开(公告)号：WO1990013802A1

公开(公告)日：1990-11-15

申请号：PCT/US1990001962

申请日：1990-04-10

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BAKER, Josefina, T. ,  MERVINE, Robert, W. ,  HUCKO, Josephine, T.

IPC: G01N01/02

CPC classification number: A61B10/0038 ,  G01N33/726

Abstract: A method and specimen slide (10) for collecting fecal specimens for occult blood testing. The slide includes a front panel (12) and back panel (14). The front panel (12) includes an aperture (18). A sheet (20) is fixed between the front (12) and back (14) panels and is positioned for receiving the fecal specimen smear through the aperture (18). The sheet (20) includes a plurality of perforations (22) that define removable portions (24) of the sheet. The hinged cover (16) can close the front panel aperture (18) and a flap (36) in the rear panel (14) may be opened to conveniently remove one or more of the removable portions (24) for testing.

Abstract translation: 用于收集粪便标本进行隐血试验的方法和标本片（10）。 滑块包括前面板（12）和后面板（14）。 前面板（12）包括孔（18）。 片（20）固定在前（12）和后（14）面板之间，并且定位成用于通过孔（18）接收粪便标本污迹。 片材（20）包括限定片材的可移除部分（24）的多个穿孔（22）。 铰接盖（16）可以关闭前面板孔（18），并且可以打开后面板（14）中的挡板（36）以方便地移除一个或多个可拆卸部分（24）用于测试。

公开(公告)号：WO8903041A3

公开(公告)日：1989-04-20

申请号：PCT/US8803368

申请日：1988-09-30

Applicant: BECKMAN INSTRUMENTS INC

Inventor： OH CHAN S ,  STERNBERG JAMES C

IPC: C07K14/00 ,  C07K1/107 ,  C07K14/575 ,  C07K16/00 ,  C07K17/00 ,  G01N33/531 ,  G01N33/532 ,  G01N33/536 ,  G01N33/539 ,  G01N33/543 ,  G01N33/94 ,  G01N33/546

CPC classification number: G01N33/54353 ,  G01N33/531 ,  G01N33/532 ,  G01N33/539 ,  G01N33/54313 ,  G01N33/94 ,  Y10S435/964 ,  Y10S436/819 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/816

Abstract: A tridentate conjugate has three chemical moieties, or tridentate members, attached through an appropriate spacer moiety. At least two of the tridentate members are relatively small molecules, usually less than about 7,000 Daltons in size. The particular appropriate spacer moiety selected for a tridentate imparts certain steric properties to the tridentate conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the tridentate members sterically inhibits the binding of a different macromolecule to another tridentate member. In another embodiment, the binding of a first tridentate member to a macromolecule restricts the subsequent binding of a second tridentate member to a proximate location on the same macromolecule.

公开(公告)号：WO8902088A3

公开(公告)日：1989-03-23

申请号：PCT/US8801657

申请日：1988-05-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： WUNDERLY STEPHEN W ,  QUINT JOSEPH F

IPC: G01T1/20 ,  G01T1/204 ,  G01T1/29

CPC classification number: G01T1/204 ,  G01T1/20

Abstract: A sample counting support for scintillation counting of a radioactive labeled sample comprises a base material covered with a thin layer of dry solid scintillator in a substantially permanent form so that the support may receive the sample and produce light emissions in response to particles emitted by a radioactive substance labelling said sample.

公开(公告)号：WO1989002089A1

公开(公告)日：1989-03-09

申请号：PCT/US1988001656

申请日：1988-05-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WUNDERLY, Stephen, W. ,  QUINT, Joseph, F.

IPC: G01T01/204

CPC classification number: G01T1/2047 ,  G01T1/204

Abstract: A method of scintillation counting utilizes a solid scintillator dispersed on a counting support (16), wherein a sample material is deposited on the counting support over the solid scintillator to produce light emissions in response to particles emitted by a radio-active substance labelling said sample.

Abstract translation: 闪烁计数的方法利用分散在计数支撑体（16）上的固体闪烁体，其中样品材料沉积在固体闪烁体上的计数支持物上，以产生响应由标记所述样品的放射性活性物质发射的颗粒的光发射 。

公开(公告)号：WO1989000689A1

公开(公告)日：1989-01-26

申请号：PCT/US1988002419

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GURSKE, William, A. ,  BLESSUM, Cynthia, R. ,  CHENG, Min-Lee ,  ANDRADE, Mark, S.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: A novel agarose gel is provided for use in the high resolution electrophoretic (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.