公开(公告)号：WO1989000689A1

公开(公告)日：1989-01-26

申请号：PCT/US1988002419

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GURSKE, William, A. ,  BLESSUM, Cynthia, R. ,  CHENG, Min-Lee ,  ANDRADE, Mark, S.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: A novel agarose gel is provided for use in the high resolution electrophoretic (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.

公开(公告)号：WO1989000688A1

公开(公告)日：1989-01-26

申请号：PCT/US1988002417

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SOHN, Chul, Ho ,  BLESSUM, Cynthia, R. ,  GAGE, Steven, C.

IPC: G01N27/26

CPC classification number: G01N27/44708

Abstract: An electrophoretic gel cooled cell including a gel supporting surface. The cell includes members for holding gels against the supporting surface such that gels of either of two dimensions may be used with the cell. A reservoir adapted to hold a cooling medium shares a common wall with the supporting surface to cool the gel retained by the members. The reservoir has a large opening which may be closed by a cover for easy insertion and removal of the cooling medium from the reservoir. A venting valve in the reservoir cover vents gas in the reservoir to atmosphere and closes when the cover is in an inverted position to prevent condensate leakage through the vent.

公开(公告)号：EP0324850B1

公开(公告)日：1992-03-18

申请号：EP88908068.5

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： SOHN, Chul, Ho ,  BLESSUM, Cynthia, R. ,  GAGE, Steven, C.

IPC: G01N27/26 ,  B01D57/02

CPC classification number: G01N27/44708

Abstract: An electrophoretic gel cooled cell including a gel supporting surface. The cell includes members for holding gels against the supporting surface such that gels of either of two dimensions may be used with the cell. A reservoir adapted to hold a cooling medium shares a common wall with the supporting surface to cool the gel retained by the members. The reservoir has a large opening which may be closed by a cover for easy insertion and removal of the cooling medium from the reservoir. A venting valve in the reservoir cover vents gas in the reservoir to atmosphere and closes when the cover is in an inverted position to prevent condensate leakage through the vent.

公开(公告)号：EP0324850A1

公开(公告)日：1989-07-26

申请号：EP88908068.0

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： SOHN, Chul, Ho ,  BLESSUM, Cynthia, R. ,  GAGE, Steven, C.

IPC: B01D57 ,  G01N27

CPC classification number: G01N27/44708

Abstract: An electrophoretic gel cooled cell including a gel supporting surface. The cell includes members for holding gels against the sup­ porting surface such that gels of either of two dimensions may be used with the cell. A reservoir adapted to hold a cooling medium shares a common wall with the supporting surface to cool the gel retained by the members. The reservoir has a large opening which may be closed by a cover for easy insertion and removal of the cooling medium from the reservoir. A venting valve in the reservoir cover vents gas in the reservoir to atmosphere and closes when the cover is in an inverted position to prevent condensate leakage through the vent.

公开(公告)号：EP0328579B1

公开(公告)日：1991-10-30

申请号：EP88906539.7

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： GURSKE, William, A. ,  BLESSUM, Cynthia, R. ,  CHENG, Min-Lee ,  ANDRADE, Mark, S.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: A novel agarose gel is provided for use in the high resolution electrophoretic (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.

公开(公告)号：EP0328579A1

公开(公告)日：1989-08-23

申请号：EP88906539.0

申请日：1988-07-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： GURSKE, William, A. ,  BLESSUM, Cynthia, R. ,  CHENG, Min-Lee ,  ANDRADE, Mark, S.

IPC: C07K14 ,  C07K1 ,  G01N27

CPC classification number: G01N27/44747

Abstract: A novel agarose gel is provided for use in the high resolution electrophoresis (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.