公开(公告)号：US20190324014A1

公开(公告)日：2019-10-24

申请号：US16400600

申请日：2019-05-01

Applicant: Creatv MicroTech, Inc.

Inventor： Cha-Mei TANG

IPC: G01N33/50 ,  B01D39/16 ,  B01L3/00 ,  B01L9/00 ,  B01D29/00 ,  G01N1/40

Abstract: A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications.

公开(公告)号：US10247725B2

公开(公告)日：2019-04-02

申请号：US14404403

申请日：2013-05-31

Applicant: CREATV MICROTECH, INC.

Inventor： Daniel Adams ,  Cha-Mei Tang

IPC: G01N33/50 ,  G01N33/574

Abstract: A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of carcinomas, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US20140322743A1

公开(公告)日：2014-10-30

申请号：US14359467

申请日：2012-11-21

Applicant: Creatv Microtech, Inc.

Inventor： Cha-Mei Tang ,  Yunqi Zhang

IPC: G01N33/50 ,  B01D39/16 ,  B01L3/00 ,  B01L9/00

CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

Abstract: A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. Unique filter holder designs and methods appropriate to hold microfilters to collect the rare cells and allow performing assays in the filter holder are provided. The invention also describes the use of the microfilter and filter holder to collect rare cells from body fluids and perform assays. Rare cells collected on the microfilter in accordance with embodiments of the present invention can be used for medical and biological research applications.

Abstract translation: 一种包含由环氧基可光定影干膜形成的聚合物层的微过滤器，以及遍及聚合物层的多个孔。 一种包含由环氧基可光限制的干膜形成的两个或更多个聚合物层的微过滤器，以及各自延伸穿过聚合物层的多个孔或开口区域。 还公开了一种形成微过滤器的方法。 该方法包括提供设置在基底上的第一层环氧基光可定义干膜，将第一层暴露于能量通过掩模，以在第一层干膜中形成由掩模限定的图案， 从曝光的第一层干膜，具有延伸穿过其中的多个孔的聚合物层，所述多个孔具有由图案限定的分布，以及从基底去除聚合物层。 提供了独特的过滤器支架设计和适用于容纳微过滤器以收集稀有细胞并允许在过滤器保持器中进行测定的方法。 本发明还描述了微过滤器和过滤器保持器从体液中收集稀有细胞并进行测定的用途。 根据本发明的实施方案收集在微过滤器上的稀有细胞可用于医学和生物研究应用。

公开(公告)号：US20030228053A1

公开(公告)日：2003-12-11

申请号：US10429258

申请日：2003-05-05

Applicant: Creatv MicroTech, Inc.

Inventor： Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

IPC: G06T007/00

CPC classification number: G06T7/55 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

Abstract translation: 公开了一种用于获取并从一系列二维（2D）图像中获得三维（3D）图像的仪器，该图像在不移动目标，检测器或聚焦透镜的相对位置的情况下获得。 2D图像由在光学系统的中心处的孔径获得的一个中心图像和在离轴位置处具有孔径获得的至少两个定向图像组成。 图像可以同时或顺序获得。 通过使用光学系统的点扩散函数的计算方法对模糊的2D图像进行分割，得到3D对象的一组去耦2D层。 然后通过使用点扩散函数的去卷积来锐化分层图像。 显示3D重建图像。 该技术提供快速数据采集和快速图像重建，并消除与运动，光毒性和光漂白相关的问题。

公开(公告)号：WO2019178226A1

公开(公告)日：2019-09-19

申请号：PCT/US2019/022050

申请日：2019-03-13

Applicant: CREATV MICROTECH, INC.

Inventor： ADAMS, Daniel ,  TANG, Cha-Mei

IPC: G01N33/574

Abstract: Means for monitoring treatment response and disease progression in subjects are disclosed, where the predictions are based on the change of number and/or size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

公开(公告)号：WO2018151865A1

公开(公告)日：2018-08-23

申请号：PCT/US2018/000082

申请日：2018-02-16

Applicant: CREATV MICROTECH, INC.

Inventor： ADAMS, Daniel ,  TANG, Cha-Mei

IPC: G01N33/574

Abstract: Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number and size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

公开(公告)号：WO2016191532A1

公开(公告)日：2016-12-01

申请号：PCT/US2016/034261

申请日：2016-05-26

Applicant: CREATV MICROTECH INC.

Inventor： ADAMS, Daniel ,  TANG, Cha-Mei

IPC: G01N33/50 ,  G01N33/574

CPC classification number: G01N33/574 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/57415 ,  G01N33/57423 ,  G01N33/5743 ,  G01N33/57434 ,  G01N33/57438 ,  G01N33/57446 ,  G01N2800/52 ,  G01N2800/56 ,  G01N2800/7028

Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

Abstract translation: 循环肿瘤细胞（CTC）与患者恶性实体瘤的转移相关。 这里提出的证据表明，CTC表现出细胞周期相位变异性，并且在有丝分裂细胞周期阶段中CTC的数量与从其获得细胞的受试者的长期存活的前景之间存在很强的相关性。 本文还介绍了确定来自患有癌症的患者的CTC的有丝分裂细胞周期阶段并使用分级恶性实体瘤的信息并预测患者存活的可能性的方法。

公开(公告)号：WO2013078409A3

公开(公告)日：2014-12-18

申请号：PCT/US2012066390

申请日：2012-11-21

Applicant: CREATV MICROTECH INC

Inventor： TANG CHA-MEI ,  ZHANG YUNQI

IPC: B01D29/00

CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

Abstract: A micro filter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A micro filter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a micro filter is also disclosed. The method includes providing a first layer of epoxy-based photodefinable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate.

Abstract translation: 一种微过滤器，包括由环氧基可光定影干膜形成的聚合物层，以及多个各自延伸穿过聚合物层的孔。 一种微过滤器，包括由环氧基可光限制的干膜形成的两个或更多个聚合物层，以及多个孔或开放区域，每个穿过聚合物层延伸。 还公开了一种形成微滤器的方法。 该方法包括提供设置在基底上的第一层环氧基可光限定干膜，将第一层暴露于能量通过掩模，以在第一层干膜中形成由掩模限定的图案，从 暴露的第一层干膜，具有延伸穿过其中的多个孔的聚合物层，所述多个孔具有由图案限定的分布，以及从基底去除聚合物层。

公开(公告)号：WO2011139445A1

公开(公告)日：2011-11-10

申请号：PCT/US2011/030966

申请日：2011-04-01

Applicant: CREATV MICROTECH, INC. ,  MAKAROVA, Olga V. ,  TANG, Cha-Mei ,  AMSTUTZ, Platte T

Inventor： MAKAROVA, Olga V. ,  TANG, Cha-Mei ,  AMSTUTZ, Platte T

IPC: B01D39/00

CPC classification number: B01D39/1692 ,  B01D67/0027 ,  B01D67/0034 ,  B01D67/0088 ,  B01D69/12 ,  B01D71/46 ,  B01D2239/0478 ,  B01D2323/345 ,  B01D2325/08

Abstract: A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate.

Abstract translation: 一种包含由环氧基可光定影干膜形成的聚合物层的微过滤器，以及遍及聚合物层的多个孔。 还公开了一种形成微过滤器的方法。 该方法包括提供设置在基底上的第一层环氧基光可定义干膜，将第一层暴露于能量通过掩模，以在第一层干膜中形成由掩模限定的图案， 从曝光的第一层干膜，具有延伸穿过其中的多个孔的聚合物层，所述多个孔具有由图案限定的分布，以及从基底去除聚合物层。

公开(公告)号：WO2010027729A2

公开(公告)日：2010-03-11

申请号：PCT/US2009/054749

申请日：2009-08-24

Applicant: CREATV MICROTECH, INC. ,  ZHU, Peixuan

Inventor： ZHU, Peixuan

IPC: A61K39/095 ,  C07K14/22 ,  C12N15/31 ,  G01N33/53

CPC classification number: G01N33/571 ,  A61K39/00 ,  C07K14/22 ,  Y02A50/472

Abstract: The present invention relates to immunogenic compositions comprising a recombinant Neisseria gonorrhoeae OpcA and methods of eliciting an immune response in a mammal by administering a formulation comprising N. gonorrhoeae OpcA or a portion or fragment of N. gonorrhoeae OpcA. The invention also provides for methods and kits for diagnosing N. gonorrhoeae infection using said recombinant N. gonorrhoeae OpcA.

Abstract translation: 本发明涉及包含重组淋病奈瑟氏球菌OpcA的免疫原性组合物和通过施用包含淋病奈瑟氏球菌OpcA或淋病奈瑟氏球菌OpcA的部分或片段的制剂在哺乳动物中引发免疫应答的方法。 本发明还提供了使用所述重组淋病奈瑟氏球菌OpcA诊断淋病奈瑟氏球菌感染的方法和试剂盒。