公开(公告)号：US20030228053A1

公开(公告)日：2003-12-11

申请号：US10429258

申请日：2003-05-05

Applicant: Creatv MicroTech, Inc.

Inventor： Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

IPC: G06T007/00

CPC classification number: G06T7/55 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

Abstract translation: 公开了一种用于获取并从一系列二维（2D）图像中获得三维（3D）图像的仪器，该图像在不移动目标，检测器或聚焦透镜的相对位置的情况下获得。 2D图像由在光学系统的中心处的孔径获得的一个中心图像和在离轴位置处具有孔径获得的至少两个定向图像组成。 图像可以同时或顺序获得。 通过使用光学系统的点扩散函数的计算方法对模糊的2D图像进行分割，得到3D对象的一组去耦2D层。 然后通过使用点扩散函数的去卷积来锐化分层图像。 显示3D重建图像。 该技术提供快速数据采集和快速图像重建，并消除与运动，光毒性和光漂白相关的问题。