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    UNIVERSAL PROBE ASSAY METHODS
    61.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用探针分析方法

    公开(公告)号：WO2012054933A2

    公开(公告)日：2012-04-26

    申请号：PCT/US2011/057536

    申请日：2011-10-24

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth ,  WEST, Jason A., A. ,  JONES, Robert, C.

    Inventor： LIVAK, Kenneth ,  WEST, Jason A., A. ,  JONES, Robert, C.

    IPC: C12Q1/68

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列，与靶序列5'的第一探针结合序列和第二探针结合序列3的扩增产物来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物

    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS
    62.
    发明申请
    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS 审中-公开
    用于检测核酸变异体的多重扩增

    公开(公告)号：WO2011085491A1

    公开(公告)日：2011-07-21

    申请号：PCT/CA2011/000054

    申请日：2011-01-14

    Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  FLUIDIGM CORPORATION ,  HANSEN, Carl, L., G. ,  PETRIV, Oleh ,  HEYRIES, Kevin ,  LIVAK, Kenneth, J.

    Inventor： HANSEN, Carl, L., G. ,  PETRIV, Oleh ,  HEYRIES, Kevin ,  LIVAK, Kenneth, J.

    IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

    CPC classification number: C12Q1/6851 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/159

    Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

    Abstract translation: 本文提供了试剂盒，引物和方法，用于通过将生物样品分配到离散的子样品中来检测生物样品中的相对目标来源参考源比例，其中生物样品包括目标源上的多个靶分子; 和参考源上的多个参考分子; 提供指向所述多个靶分子中的一个或多个的靶引物和指向所述多个参考分子中的一个或多个的参考引物; 用目标引物和参照引物进行数字扩增; 并用靶探针检测扩增的靶产物的存在或不存在，并用参考探针检测扩增的参考产物的存在或不存在，其中扩增的靶产物与扩增的参考产物的比率指示靶源与参考源的相对量 在生物样品中。

    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS
    65.
    发明申请
    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS 审中-公开
    复制数变化确定，方法和系统

    公开(公告)号：WO2009033178A1

    公开(公告)日：2009-03-12

    申请号：PCT/US2008/075636

    申请日：2008-09-08

    Applicant: FLUIDIGM CORPORATION ,  RAMAKRISHNAN, Ramesh

    Inventor： RAMAKRISHNAN, Ramesh

    IPC: G01N33/48

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    Abstract translation: 用于确定受试者的基因组中的靶多核苷酸的拷贝数变异的本发明的方法和系统，包括基于扩增的技术。 方法可以包括样品的预扩增，随后分布样品和多个反应体积，定量检测靶多核苷酸和参考多核苷酸，并进行分析，以确定基因组中靶多核苷酸序列的相对拷贝数 的主题。

    METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS
    66.
    发明申请
    METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS 审中-公开
    生物样品分析方法与装置

    公开(公告)号：WO2008067552A2

    公开(公告)日：2008-06-05

    申请号：PCT/US2007/086146

    申请日：2007-11-30

    Applicant: FLUIDIGM CORPORATION ,  SUN, Gang ,  RAMAKRISHNAN, Ramesh ,  JONES, Robert C.

    Inventor： SUN, Gang ,  RAMAKRISHNAN, Ramesh ,  JONES, Robert C.

    IPC: G06T11/20

    CPC classification number: C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

    Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

    Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

    MICROFLUIDIC CHECK VALVES
    67.
    发明申请
    MICROFLUIDIC CHECK VALVES 审中-公开
    微流感检查阀

    公开(公告)号：WO2008043046A2

    公开(公告)日：2008-04-10

    申请号：PCT/US2007/080489

    申请日：2007-10-04

    Applicant: FLUIDIGM CORPORATION ,  WANG, Jing ,  NASSEF, Hany, R.

    Inventor： WANG, Jing ,  NASSEF, Hany, R.

    IPC: B01J19/00

    CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0887 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0001 ,  F16K99/0057 ,  F16K2099/0078 ,  F16K2099/008

    Abstract: The present invention is a robust, microfluidic check valve and a method of using the check valve in microfluidic devices. The check valve is comprised two stacked chambers that are separated by a pore-containing membrane. The membrane is composed of an elastomeric material and can be configured in normally open or normally closed state. The normally open check valve can be implemented so that the degree of back pressure necessary to close the valve can be set. The normally closed embodiment can maintain a closed state with essentially no back pressure. Both the normally open and the normally closed version can be readily produced by multilayer soft lithographic techniques and may retain effective functioning through many thousands of opening and closing cycles without failure. Such check valves can substitute for active valve structures in microfluidic devices and, when appropriately implemented, can simplify the design, manufacture, and/or operation of the devices containing them.

    Abstract translation: 本发明是一种坚固的微流体止回阀和在微流体装置中使用止回阀的方法。 止回阀包括由含孔膜隔开的两个堆叠室。 膜由弹性体材料构成，并且可以构造成常开或常闭状态。 可以实现常开止回阀，使得可以设定关闭阀所需的背压程度。 常闭实施例可以保持基本上没有背压的关闭状态。 常开和常闭型都可以通过多层软平版印刷技术轻松生产，并可通过数千个开闭周期保持有效的功能，而不会发生故障。 这种止回阀可以替代微流体装置中的主动阀结构，并且当适当实施时，可以简化包含它们的装置的设计，制造和/或操作。

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME
    69.
    发明申请
    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME 审中-公开
    晶体形成装置和系统及其制造和使用方法

    公开(公告)号：WO2005072353A2

    公开(公告)日：2005-08-11

    申请号：PCT/US2005/002408

    申请日：2005-01-25

    Applicant: FLUIDIGM CORPORATION ,  GROSSMAN, Robert ,  UNGER, Marc ,  LAM, Phillip ,  CHOU, Hou-Pu ,  KIMBALL, Jake ,  PIEPRZYK, Martin

    Inventor： GROSSMAN, Robert ,  UNGER, Marc ,  LAM, Phillip ,  CHOU, Hou-Pu ,  KIMBALL, Jake ,  PIEPRZYK, Martin

    IPC: B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58

    CPC classification number: C12Q1/68 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/11

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    Abstract translation: 本发明提供了微流体装置及其使用方法。 本发明还提供了用于使用微流体装置的装置和系统，分析在微流体装置中进行的反应，以及用于生成，存储，组织和分析使用微流体装置产生的数据的系统。 本发明还提供了使用和制造微流体系统和装置的方法，在一些实施方案中，它们可用于晶体形成。 在一个实施例中，一种装置包括具有其中具有一个或多个流体端口的压板面的压板。 流体端口在空间上对应于微流体装置表面上的一个或多个孔。 包括用于相对于压板保持微流体装置的平台，以及用于将压板压靠在微流体装置上的压板致动器，使得压板的至少一个流体端口被推压到一个井中以形成压力室 包括井和端口，使得当压力流体通过其中一个端口被引入或从压力室移除时，流体压力在其中改变。

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