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    DIGITAL ANALYTE ANALYSIS
    64.
    发明申请
    DIGITAL ANALYTE ANALYSIS 审中-公开
    数字分析分析

    公开(公告)号：WO2013165748A1

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/037751

    申请日：2013-04-23

    Applicant: RAINDANCE TECHNOLOGIES, INC

    Inventor： HUTCHISON, John, Brian ,  OLSON, Jeffrey, Charles ,  LINK, Darren, Roy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/686 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2535/122

    Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided. In one aspect, the invention provides a droplet that contains a single nucleic acid template and a plurality of primer pairs specific for multiple target sites on the template. The single nucleic acid template can be DNA or RNA. The template is amplified in the droplet for detection; and may preferably be amplified using a plurality of primer pairs as described herein. The ability to amplify and detect single nucleic acids in droplets enables digital PCR, detection, counting, and differentiation among nucleic acids, especially those present in heterogeneous samples. Thus, the invention applies to digital amplification techniques and, in specific embodiments enables multiplex PCR in droplets.

    Abstract translation: 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。 在一个方面，本发明提供了包含单一核酸模板和对模板上多个靶位点特异的多个引物对的液滴。 单个核酸模板可以是DNA或RNA。 模板在液滴中被放大以便检测; 并且优选地可以使用如本文所述的多个引物对来扩增。 在液滴中扩增和检测单个核酸的能力使得能够在核酸之间进行数字PCR，检测，计数和分化，特别是存在于异质样品中的核酸。 因此，本发明适用于数字放大技术，并且在具体实施例中使得能够以液滴方式进行多重PCR。

    SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS
    68.
    发明申请
    SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS 审中-公开
    用于处理微流感滴液的系统和方法

    公开(公告)号：WO2014165559A2

    公开(公告)日：2014-10-09

    申请号：PCT/US2014/032614

    申请日：2014-04-02

    Applicant: RAINDANCE TECHNOLOGIES, INC.

    Inventor： LINK, Darren, R.

    IPC: G01N1/38 ,  C12Q1/68

    CPC classification number: B01L3/502715 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N35/085

    Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

    Abstract translation: 本发明一般涉及用于从容器移动液滴的组件，其有助于液滴的收集和转移，同时最小化样品损失。 在某些方面，组件包括至少一个液滴形成模块，其中模块被配置成形成由不混溶流体包围的液滴。 组件还包括至少一个包括出口的室，其中室被构造成接收液滴和不混溶的流体，并且其中出口构造成仅基本上容纳液滴。 组件还包括通道，其被构造成使得液滴形成模块和腔室经由通道彼此流体连通。 在其他方面，组件包括多个中空构件，其中中空构件是通道，构件中构件与容器相互作用。 多个中空构件包括构造成排出与容器中的液滴不混溶的流体的第一构件和构造成基本上仅从容器中滴下的第二构件。 组件还包括主通道，其中第二构件与主通道流体连通。 组件还包括连接到主通道的至少一个分析模块。

    SANDWICH ASSAYS IN DROPLETS
    70.
    发明申请
    SANDWICH ASSAYS IN DROPLETS 审中-公开
    滴灌中的三重测定

    公开(公告)号：WO2012045012A3

    公开(公告)日：2012-07-19

    申请号：PCT/US2011054353

    申请日：2011-09-30

    Applicant: RAINDANCE TECHNOLOGIES INC ,  SAMUELS MICHAEL ,  LINK DARREN

    Inventor： SAMUELS MICHAEL ,  LINK DARREN

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: G01N33/54366 ,  C12Q1/6874

    Abstract: Methods of performing sandwich assays in droplets are disclosed. Further disclosed are methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte; separating the complexes, and detecting the complexes, thereby detecting the target analyte.

    Abstract translation: 公开了以液滴形式进行夹心测定的方法。 进一步公开的是用于检测目标分析物的方法，其包括形成包含疑似含有目标分析物的样品的一部分和样品标识符的流体的分隔部分，具有目标标识的第一结合剂和特异性的第二结合剂 在产生第一和第二结合剂与目标分析物的复合物的条件下的目标分析物; 分离复合物，检测复合物，从而检测目标分析物。

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