公开(公告)号：JP2014138611A

公开(公告)日：2014-07-31

申请号：JP2014059522

申请日：2014-03-24

Applicant: Raindance Technologies Inc ,  レインダンス テクノロジーズ, インコーポレイテッド

Inventor： LINK DARREN R ,  LAURENT BOITARD ,  JEFFREY BRANCIFORTE ,  YVES CHARLES ,  GILBERT FEKE ,  JOHN Q LU ,  DAVID MARRAN ,  AHMADALI TABATABAI ,  MICHAEL WEINER ,  WOLFGANG HINZ ,  JONATHAN M ROTHBERG

IPC: C12Q1/68 ,  G01N35/08 ,  G01N37/00

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: PROBLEM TO BE SOLVED: To provide a method for detecting a polymerase chain reaction (PCR) product in aqueous droplets.SOLUTION: The method for detecting a polymerase chain reaction (PCR) product in aqueous droplets comprises the steps of: forming a plurality of aqueous droplets which have uniform capacity in a microfluidic chip: moving the plurality of aqueous droplets to an off-chip reservoir in which at least one of the aqueous droplets contains a reagent for nucleic acid and PCR reaction; performing PCR reaction while the aqueous droplets are present in the off-chip reservoir; moving the plurality of aqueous droplets from the off-chip reservoir to a channel; adding an immiscible carrier fluid between the aqueous droplets, thereby increasing intervals between the aqueous droplets in the channel; and detecting the PCR product in one or more of the aqueous droplets in the channel.

Abstract translation: 要解决的问题：提供一种用于在水滴中检测聚合酶链反应（PCR）产物的方法。溶液：用于在水滴中检测聚合酶链反应（PCR）产物的方法包括以下步骤：形成多个水溶液 在微流体芯片中具有均匀容量的液滴：将多个水滴移动到片外储存器，其中至少一个水滴含有用于核酸和PCR反应的试剂; 在液滴存在于片外储存器中时执行PCR反应; 将所述多个水滴从所述芯片外储存器移动到通道; 在水滴之间添加不混溶的载液，从而增加通道中的水滴之间的间隔; 以及在通道中的一个或多个水滴中检测PCR产物。

公开(公告)号：JP2013015536A

公开(公告)日：2013-01-24

申请号：JP2012204765

申请日：2012-09-18

Applicant: Raindance Technologies Inc ,  レインダンス テクノロジーズ, インコーポレイテッド

Inventor： LINK DARREN R ,  MICHAEL WEINER ,  DAVID MARREN ,  JONATHAN M ROTHBERG

IPC: G01N35/08 ,  C12M1/00 ,  G01N37/00

CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149

Abstract: PROBLEM TO BE SOLVED: To provide microfluidic devices.SOLUTION: The present invention provides substrates having individual fluid handling modules that can be combined into fluid processing systems so as to perform multi-step processing of isolated components, which is essential to perform biological, chemical and diagnostic applications, quickly, effectively and inexpensively. Using principles based on electrical manipulation of droplets, the microfluidic substrates can encapsulate reagents into droplets, which can be combined, analyzed, and sorted.

Abstract translation: 要解决的问题：提供微流体装置。 解决方案：本发明提供了具有单独的流体处理模块的基板，其可以组合成流体处理系统，以便执行隔离部件的多步骤处理，这对于快速有效地执行生物，化学和诊断应用是至关重要的 并且成本低廉。 使用基于液滴的电操作的原理，微流体底物可以将试剂包封成液滴，其可以组合，分析和分选。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：AU2006335290A1

公开(公告)日：2007-07-19

申请号：AU2006335290

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： FEKE GILBERT ,  CHARLES YVES ,  LINK DARREN R ,  HINZ WOLFGANG ,  ROTHBERG JONATHAN M ,  TABATABAI AHMADALI ,  MARRAN DAVID ,  WEINER MICHAEL ,  LU JOHN Q ,  BRANCIFORTE JEFFREY ,  BOITARD LAURENT

IPC: G01N33/536 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N33/68

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscrible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

公开(公告)号：CA2636855A1

公开(公告)日：2007-07-19

申请号：CA2636855

申请日：2006-06-01

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： HINZ WOLFGANG ,  WEINER MICHAEL ,  LINK DARREN R ,  TABATABAI AHMADALI ,  MARRAN DAVID ,  ROTHBERG JONATHAN M ,  LU JOHN Q ,  FEKE GILBERT ,  CHARLES YVES ,  BRANCIFORTE JEFFREY ,  BOITARD LAURENT

IPC: G01N33/536 ,  B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N33/68

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscrible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

公开(公告)号：CA2789425A1

公开(公告)日：2011-08-18

申请号：CA2789425

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12Q1/68 ,  C12N15/10 ,  C12P19/34 ,  C40B30/04

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：AT540750T

公开(公告)日：2012-01-15

申请号：AT08170992

申请日：2007-05-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LINK DARREN R

IPC: B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  G01N15/14 ,  G01N33/50

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US10139411B2

公开(公告)日：2018-11-27

申请号：US15415290

申请日：2017-01-25

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/58 ,  G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  B01L7/00 ,  G01N21/64

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US09944977B2

公开(公告)日：2018-04-17

申请号：US14568923

申请日：2014-12-12

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link ,  Michael Samuels

IPC: C12Q1/68 ,  C07H21/02

CPC classification number: C12Q1/6844 ,  C12Q1/6858 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

公开(公告)号：US20180080020A1

公开(公告)日：2018-03-22

申请号：US15694108

申请日：2017-09-01

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/68 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  B01L7/00 ,  B01F3/08

CPC classification number: B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US20180057863A1

公开(公告)日：2018-03-01

申请号：US15688250

申请日：2017-08-28

Applicant: Raindance Technologies, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.