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    NUCLEIC ACID PROBE ARRAYS
    67.
    发明申请
    NUCLEIC ACID PROBE ARRAYS 审中-公开
    核酸探针阵列

    公开(公告)号：WO0138580A9

    公开(公告)日：2002-12-05

    申请号：PCT/US0032131

    申请日：2000-11-27

    Applicant: CURAGEN CORP ,  ROTHBERG JONATHAN M ,  BADER JOEL S

    Inventor： ROTHBERG JONATHAN M ,  BADER JOEL S

    IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/566 ,  G01N37/00

    CPC classification number: B82Y30/00 ,  C12Q1/682 ,  C12Q1/6853 ,  C12Q2600/156 ,  C12Q2565/519 ,  C12Q2531/125 ,  C12Q2521/501

    Abstract: Disclosed are nucleic acid probe arrays and methods of identifying and sequencing nucleic acids in a population of nucleic acids using the arrays. The method is preferably performed by annealing a nucleic acid template to an anchor primer attached to a durface of the array. At least one anchor in the array has a sequence complementary to sequences at the 5' and 3' termini of a target nucleic acid.The annealed linear target nucleic acid is circularized using one or two ligation reactions. In one embodiment, one liggation is issued. Annealing of of the linear nucleic acid results in juxtaposition of the 5' and 3' termini of the target nucleic acid on the anchor primer. Addition of a ligase results in circulization of the target nucleic acid. This circularized nucleic acid is a template for extension of the anchor primer in a rolling circle amplification reaction. An extended anchor primer containing multiple copies of a sequence complementary to the circular mucleic acid is also referred to herein as a anchor primer nucleid acid-nucleic acid concatamer. The presence of multiple copies of the complementary sequence facilitates detection of the nucleic acid. Thus, the method provides for a highly sensitive method of detecting a desired nucleic acid attached at a discrete location on the array.

    Abstract translation: 公开了核酸探针阵列和使用阵列鉴定和测序核酸群体中的核酸的方法。 该方法优选通过将核酸模板退火至连接到阵列的表面的锚定引物进行。 阵列中的至少一个锚具有与靶核酸的5'和3'末端的序列互补的序列。退火的线性靶核酸使用一个或两个连接反应进行环化。 在一个实施例中，发出一次结扎。 线性核酸的退火导致靶核酸的5'和3'末端在锚定引物上并列。 连接酶的加入导致靶核酸的循环。 该循环核酸是用于在滚环扩增反应中延伸锚定引物的模板。 包含与环状核酸互补的多个拷贝的扩增的锚定引物在本文中也称为锚定引物核酸 - 核酸连接体。 互补序列的多拷贝的存在便于检测核酸。 因此，该方法提供了检测在阵列上离散位置附着的所需核酸的高灵敏度方法。

    NUCLEIC ACID PROBE ARRAYS
    69.
    发明申请
    NUCLEIC ACID PROBE ARRAYS 审中-公开

    公开(公告)号：WO2001038580A3

    公开(公告)日：2001-05-31

    申请号：PCT/US2000/032131

    申请日：2000-11-27

    Applicant: CURAGEN CORPORATION ,  ROTHBERG, Jonathan, M. ,  BADER, Joel, S.

    Inventor： ROTHBERG, Jonathan, M. ,  BADER, Joel, S.

    IPC: C12Q1/68

    Abstract: Disclosed are nucleic acid probe arrays and methods of identifying and sequencing nucleic acids in a population of nucleic acids using the arrays. The method is preferably performed by annealing a nucleic acid template to an anchor primer attached to a durface of the array. At least one anchor in the array has a sequence complementary to sequences at the 5' and 3' termini of a target nucleic acid.The annealed linear target nucleic acid is circularized using one or two ligation reactions. In one embodiment, one liggation is issued. Annealing of of the linear nucleic acid results in juxtaposition of the 5' and 3' termini of the target nucleic acid on the anchor primer. Addition of a ligase results in circulization of the target nucleic acid. This circularized nucleic acid is a template for extension of the anchor primer in a rolling circle amplification reaction. An extended anchor primer containing multiple copies of a sequence complementary to the circular mucleic acid is also referred to herein as a anchor primer nucleid acid-nucleic acid concatamer. The presence of multiple copies of the complementary sequence facilitates detection of the nucleic acid. Thus, the method provides for a highly sensitive method of detecting a desired nucleic acid attached at a discrete location on the array.

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