公开(公告)号：WO2013081852A1

公开(公告)日：2013-06-06

申请号：PCT/US2012/065441

申请日：2012-11-16

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： GEROMANOS, Scott, J. ,  VISSERS, Johannes PC ,  LANGRIDGE, James, I.

IPC: G01N24/00 ,  G06F19/00

CPC classification number: G01N30/7206 ,  F04C2270/041 ,  G01N30/7233 ,  G01N33/68 ,  G01N33/92 ,  H01J49/0036

Abstract: Techniques are described for quantification of molecules in a sample. Mass spectrometry is performed to obtain ionization intensities for precursor and product ions originating from a particular molecule. A first set of precursor ions having the highest ionization intensities and originating from the particular molecule is determined. For each of the one or precursors in the first set, determined is a second set of one or more product ions that are fragments associated with said each precursor and have the highest ionization intensities of product ions associated with said each precursor. An intensity sum is calculated for the particular molecule by adding ionization intensities of product ions included in the second sets for the one or more precursors in the first set. The intensity sum is compared to information included in a calibration standard. A quantity of the particular molecule in the sample is determined based on said comparing.

Abstract translation: 描述了用于定量样品中分子的技术。 进行质谱以获得源自特定分子的前体和产物离子的电离强度。 确定具有最高电离强度并源于特定分子的第一组前体离子。 对于第一组中的一个或前体的每一个，确定的是与所述每个前体相关联并且与所述每个前体相关联的产物离子具有最高离子化强度的一个或多个产物离子的第二组。 通过对第一组中的一种或多种前体添加包含在第二组中的产物离子的离子化强度来计算特定分子的强度和。 将强度和与包括在校准标准中的信息进行比较。 基于所述比较确定样品中特定分子的量。

公开(公告)号：WO2013049622A1

公开(公告)日：2013-04-04

申请号：PCT/US2012/057996

申请日：2012-09-28

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： BROUSMICHE, Darryl, W. ,  YU, Ying-qing

IPC: G01N33/533 ,  C07D207/40

CPC classification number: G01N33/58 ,  C07D207/46 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08 ,  G01N33/582 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20 ,  Y10T436/143333

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

Abstract translation: 教导并描述了包含具有活性官能团的活性荧光分子的试剂，其具有用于与用于标记生物分子如N-聚糖的胺反应的活性官能团及其用途。

公开(公告)号：WO2013028765A1

公开(公告)日：2013-02-28

申请号：PCT/US2012/051876

申请日：2012-08-22

Applicant: WATERS TECHNOLOGIES CORPORATION ,  MICHIENZI, Joseph D. ,  MURPHY, James P. ,  KEENE, Russell L.

Inventor： MICHIENZI, Joseph D. ,  MURPHY, James P. ,  KEENE, Russell L.

IPC: B01D15/08

CPC classification number: G01N30/06 ,  B01L3/502715 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  G01N30/16 ,  G01N30/6095 ,  G01N30/88 ,  G01N33/49 ,  G01N2001/383 ,  G01N2030/009 ,  G01N2030/062

Abstract: An apparatus for use in a chromatography system includes a first microfluidic substrate having a first fluidic channel. One end of the first fluidic channel terminates at a first fluidic port on a first side of the first microfluidic substrate and an opposite end of the first fluidic channel terminates at a second fluidic port on a second side of the first microfluidic substrate. A second microfluidic substrate has a second fluidic channel. One end of the second fluidic channel terminates at a first fluidic port on a first side of the second microfluidic substrate. The first side of the second microfluidic substrate abuts the second side of the first microfluidic substrate such that the fluidic port of the second microfluidic substrate aligns with one of the fluidic ports of the first microfluidic substrate and the alignment produces a fluidic path comprised of the first and second fluidic channels.

Abstract translation: 用于色谱系统的装置包括具有第一流体通道的第一微流体衬底。 第一流体通道的一端终止在第一微流体衬底的第一侧上的第一流体端口处，并且第一流体通道的相对端终止于第一微流体衬底的第二侧上的第二流体端口。 第二微流体衬底具有第二流体通道。 第二流体通道的一端终止在第二微流体衬底的第一侧上的第一流体端口处。 第二微流体衬底的第一侧邻接第一微流体衬底的第二侧，使得第二微流体衬底的流体端口与第一微流体衬底的流体端口中的一个对准，并且对准产生流体路径，其包括第一微流体衬底 和第二流体通道。

公开(公告)号：WO2013028450A1

公开(公告)日：2013-02-28

申请号：PCT/US2012/051088

申请日：2012-08-16

Applicant: WATERS TECHNOLOGIES CORPORATION ,  SHREVE, Joshua A. ,  MAILLET, Maria

Inventor： SHREVE, Joshua A.

IPC: B01D15/10

CPC classification number: G01N30/30 ,  G01N30/466 ,  G01N30/54 ,  G01N30/6047 ,  G01N2030/027 ,  G01N2030/303 ,  G01N2030/8881

Abstract: A thermal system for use in a column manager of a liquid chromatography system comprises a plurality of spatially separated individually controlled thermoelectric chips. A column module houses a plurality of thermally conductive troughs. Each trough resides in a separate thermal zone to be thermally conditioned individually by one of the individually controlled thermoelectric chips. Each trough is adapted to hold one or more liquid chromatography columns therein. A plurality of spatially separated thermal bridges includes a first thermal bridge thermally coupling one of the thermoelectric chips to a first one of the plurality of troughs and a second thermal bridge of the plurality of thermal bridges thermally coupling another of the thermoelectric chips to a second one of the plurality of troughs.

Abstract translation: 用于液相色谱系统的柱管理器的热系统包括多个空间分离的独立控制的热电芯片。 列模块容纳多个导热槽。 每个槽位于单独的热区中，由单独控制的热电芯片之一分别进行热调节。 每个槽适于在其中容纳一个或多个液相色谱柱。 多个空间分离的热桥包括将热电芯片中的一个热耦合到多个槽中的第一个槽的第一热桥和多个热桥中的另一个热桥将热耦合到第二热桥的第二热桥 的多个槽。

公开(公告)号：WO2012174077A1

公开(公告)日：2012-12-20

申请号：PCT/US2012/042194

申请日：2012-06-13

Applicant: WATERS TECHNOLOGIES CORPORATION ,  YURACH, Dana ,  BASTEK, Peter ,  RISTACHE, Catalin

Inventor： YURACH, Dana ,  BASTEK, Peter ,  RISTACHE, Catalin

IPC: G06F15/16

CPC classification number: G06Q30/018 ,  G06F11/3055

Abstract: Described are techniques for determining a qualification status of a device in a system. An occurrence of a trigger event for the device is determined. The trigger event is caused by an occurrence of any of a time -based event, a performance-based event, a usage -based event, and an unscheduled event. A user notification is provided to perform a first action responsive to the occurrence. The first action is an action to perform any of a maintenance activity, a repair activity, and a test for the device. The qualification status of the device is updated in accordance with said first action. Also described are techniques for more generally determining a compliance status of a device where the compliance status may be related to any one or more of qualification, verification, validation and/or calibration of the device.

Abstract translation: 描述了用于确定系统中设备的资格状态的技术。 确定设备的触​​发事件的发生。 触发事件是由任何基于时间的事件，基于性能的事件，基于使用的事件和非计划事件的发生引起的。 提供用户通知以响应于该发生执行第一动作。 第一个动作是执行任何维护活动，修复活动和设备测试的操作。 根据所述第一动作更新设备的资格状态。 还描述了用于更一般地确定设备的符合性状态的技术，其中遵从状态可以与设备的限定，验证，验证和/或校准中的任何一个或多个有关。

公开(公告)号：WO2012170756A1

公开(公告)日：2012-12-13

申请号：PCT/US2012/041451

申请日：2012-06-08

Applicant: WATERS TECHNOLOGIES CORPORATION ,  BUNNER, Bernard

Inventor： BUNNER, Bernard

IPC: G01N30/38

CPC classification number: B01D15/22 ,  B01L3/502 ,  B01L2200/027 ,  B32B38/10 ,  G01N30/6086 ,  G01N30/6095 ,  Y10T156/1064

Abstract: A planar microfluidic chemical separation device includes a separation channel that is located in the plane of the device. The device also includes one or more vias situated perpendicular to the separation channel. The vias extends between the separation channel and an outer surface of the substrate for fluid communication with the separation channel. The vias have cross-sectional areas that are substantially less than a cross-sectional area of a first region of the separation channel to inhibit band-broadening caused by passage of a sample band through the one or more vias.

Abstract translation: 平面微流体化学分离装置包括位于装置的平面中的分离通道。 该装置还包括垂直于分离通道定位的一个或多个通孔。 通孔在分离通道和衬底的外表面之间延伸，用于与分离通道流体连通。 通孔具有基本上小于分离通道的第一区域的横截面面积的截面积，以阻止由于样品带通过一个或多个通孔而导致的带宽变形。

公开(公告)号：WO2012170549A1

公开(公告)日：2012-12-13

申请号：PCT/US2012/041124

申请日：2012-06-06

Applicant: WATERS TECHNOLOGIES CORPORATION ,  COOPER, Donald, P.

Inventor： COOPER, Donald, P.

IPC: G01N30/86

CPC classification number: H01J49/0009 ,  G01N30/72 ,  G01N30/8665 ,  G01N2030/045

Abstract: A method of quantifying a target analyte by mass spectrometry includes obtaining a mass spectrometer signal comprising a first calibrator signal, comprising a second calibrator signal, and potentially comprising a target analyte signal from a single sample comprising a first known quantity of a first calibrator, comprising a second known quantity of a second calibrator, and potentially comprising a target analyte. The first known quantity and the second known quantity are different, and wherein the first calibrator, the second calibrator, and the target analyte are each distinguishable in the single sample by mass spectrometry. The method also includes quantifying the target analyte in the single sample using the first calibrator signal, the second calibrator signal, and the target analyte signal.

Abstract translation: 通过质谱法对目标分析物进行定量的方法包括获得包括第一校准器信号的质谱仪信号，包括第二校准器信号，潜在地包括来自包含第一已知量的第一校准器的单个样品的目标分析物信号，包括 第二已知量的第二校准器，并且潜在地包含靶分析物。 第一已知量和第二已知量是不同的，并且其中第一校准器，第二校准器和目标分析物在单个样品中通过质谱分别可分辨。 该方法还包括使用第一校准器信号，第二校准器信号和目标分析物信号量化单个样品中的目标分析物。

公开(公告)号：WO2012148794A2

公开(公告)日：2012-11-01

申请号：PCT/US2012/034343

申请日：2012-04-20

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： CHEN, Carl, Gang ,  JENCKS, Robert, A. ,  LU, Wen ,  MOELLER, Mark, W.

IPC: F16K25/04

CPC classification number: F16K25/005 ,  C23C14/0605 ,  F16K11/0655 ,  F16K11/0743 ,  G01N30/16 ,  G01N30/20 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/207 ,  Y10T137/86638 ,  Y10T137/86863

Abstract: A shear valve for use in a high performance liquid chromatography system. The shear valve includes a first valve member having a plurality of first fluid-conveying features, and a second valve member having one or more second fluid-conveying features. The second valve member is movable, relative to the first valve member, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid- conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. At least one of the first and second valve members is at least partially coated with a protective coating that includes an adhesion interlayer and a diamond-like carbon (DLC) layer. The DLC layer is deposited on the adhesion interlayer via filtered cathodic vacuum arc (FCVA) deposition.

Abstract translation: 用于高效液相色谱系统的剪切阀。 剪切阀包括具有多个第一流体输送特征的第一阀构件和具有一个或多个第二流体输送特征的第二阀构件。 第二阀构件可相对于第一阀构件在多个离散位置之间移动，使得在每个离散位置中，一个或多个第二流体输送特征中的至少一个与第一流体输送特征中的多个位置重叠 流体输送特征以提供其间的流体连通。 第一和第二阀构件中的至少一个至少部分地涂覆有包括粘附中间层和类金刚石碳（DLC）层的保护涂层。 通过过滤阴极真空电弧（FCVA）沉积将DLC层沉积在粘附中间层上。

公开(公告)号：WO2012109103A2

公开(公告)日：2012-08-16

申请号：PCT/US2012/023759

申请日：2012-02-03

Applicant: WATERS TECHNOLOGIES CORPORATION ,  MOELLER, Mark, W. ,  CIOLKOSZ, Theodore, D.

Inventor： MOELLER, Mark, W. ,  CIOLKOSZ, Theodore, D.

IPC: G01N30/38

CPC classification number: G01N30/38 ,  F16K11/10 ,  G01N30/20 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/204 ,  Y10T137/87909

Abstract: A fluid switching valve includes a first valve element (e.g., a stator) that has a plurality of first fluid-conveying features (e.g., ports), and a second valve element (e.g., a rotor) that has one or more second fluid-conveying features (e.g., fluid conduits in the form of grooves). The second valve element is movable, relative to the first valve element, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. At least one of the first valve element and the second valve element includes a recess. The recess serves to reduce wear between the first valve element and the second valve element. The recess is arranged such that it does not overlap with any of the first fluid-conveying features or any of the second fluid-conveying features when the rotor is in any of the discrete positions.

Abstract translation: 流体切换阀包括具有多个第一流体输送特征（例如，端口）的第一阀元件（例如，定子）和具有一个或多个第二流体输送特征（例如，端口）的第二阀元件（例如，转子） 输送特征（例如，凹槽形式的流体导管）。 第二阀元件相对于第一阀元件可在多个离散位置之间移动，使得在每个离散位置中，一个或多个第二流体输送特征中的至少一个与第一流体输送特征中的多个位置重叠 流体输送特征以提供其间的流体连通。 第一阀元件和第二阀元件中的至少一个包括凹部。 凹部用于减少第一阀元件和第二阀元件之间的磨损。 凹部被布置成当转子处于任何离散位置时，其不与第一流体输送特征或任何第二流体输送特征中的任何一个重叠。

公开(公告)号：WO2012040178A1

公开(公告)日：2012-03-29

申请号：PCT/US2011/052319

申请日：2011-09-20

Applicant: WATERS TECHNOLOGIES CORPORATION ,  LECLAIR, Steven ,  RAMIREZ, Enrique ,  YURACH, Dana ,  TURNER, Laura ,  CASWELL, Allen ,  ROBINSON, Mark

Inventor： LECLAIR, Steven ,  RAMIREZ, Enrique ,  YURACH, Dana ,  TURNER, Laura ,  CASWELL, Allen ,  ROBINSON, Mark

IPC: G06F9/44

CPC classification number: G06F11/3668 ,  G06F9/44 ,  G06F9/455 ,  G06F11/3672

Abstract: Described are techniques for testing an application. A test script including one or more instructions is received to test an application. A user interaction with a user interface of the application is emulated. Emulating includes executing a first portion of instructions in the test script. An output is received including information displayed using the user interface in response to the user interaction. Verification processing is performed for the output. The verification processing includes executing a second portion of instructions in the test script. A first instruction in the test script references a user interface data element without specifying a complete path for the user interface data element.

Abstract translation: 描述了测试应用程序的技术。 接收到包含一个或多个指令的测试脚本来测试应用程序。 模拟用户与应用程序的用户界面的交互。 仿真包括在测试脚本中执行指令的第一部分。 接收包括响应于用户交互的使用用户界面显示的信息的输出。 对输出执行验证处理。 验证处理包括在测试脚本中执行指令的第二部分。 测试脚本中的第一条指令引用用户界面数据元素，而不指定用户界面数据元素的完整路径。