公开(公告)号：KR1020110086262A

公开(公告)日：2011-07-28

申请号：KR1020100005895

申请日：2010-01-22

Applicant: 대한민국(농촌진흥청장)

Inventor： 방경환 ,  김영창 ,  현동윤 ,  김옥태 ,  배영석 ,  김동휘 ,  김영철 ,  이제완 ,  조익현 ,  서아연

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6844 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: PURPOSE: A method for determining a STS(sequence tagged site) nucleic acid marker is provided to simply distinguish ginseng kind by genetic pattern. CONSTITUTION: A STS primer set for determining ginseng kind contains: an oligonucleotide primer set of sequence numbers 1 and 2; an oligonucleotide primer set of sequence numbers 3 and 4; an oligonucleotide primer set of sequence numbers 5 and 6; or an oligonucleotide primer set of sequence numbers 7 and 8. The ginseng kind is Chunpoong, Yunpoong, Gopoong, Kumpoong, Sunpoong, or Sunwon. The STS primer set is derived from Panax ginseng C. A. Meyer. A kit for determining the ginseng kind contains the primer set, and reagent for amplification. A method for determining the ginseng kind comprises: a step of isolating genome DNA from a ginseng sample; a step of amplifying a target sequence using the primer set by amplification; and a step of detecting amplified products.

Abstract translation: 目的：提供一种用于确定STS（序列标记位点）核酸标记物的方法，以通过遗传模式简单区分人参种类。 构成：用于确定人参种类的STS引物组包含：序列号1和2的寡核苷酸引物组; 序列号3和4的寡核苷酸引物组; 序列号5和6的寡核苷酸引物组; 或序列号7和8的寡核苷酸引物组。人参种类为春蓬，云泊，Gopoong，Kumpoong，Sunpoong或Sunwon。 STS引物组来自人参C.A.Meyer。 用于测定人参种的试剂盒包含引物组和扩增试剂。 确定人参种的方法包括：从人参样品中分离基因组DNA的步骤; 使用扩增引物组扩增靶序列的步骤; 以及检测扩增产物的步骤。