公开(公告)号：KR100374482B1

公开(公告)日：2003-03-03

申请号：KR1020020005448

申请日：2002-01-30

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  권익찬 ,  정혜선

IPC: A61K9/10

CPC classification number: A61K9/5123 ,  A61K9/1075 ,  A61K9/1272 ,  A61K48/00 ,  C12N15/88

Abstract: The present invention relates to oil-in-water lipid emulsions composed of non-triglyceride oils and solid lipid nanoparticles (SLN) composed of triglyceride or ethyl stearate used as gene transfection agents and drug delivery systems and method for preparing thereof. The present invention also concerns the method of transferring genes or drugs efficiently into cells by using the lipid emulsions and solid lipid nanoparticles. Also the present invention relates to the method of preparing lipid emulsions containing lipophilic or amphiphilic drugs by using squalene or squalane as the core-oil. The present invention also concerns the method of preparing the solid lipid nanoparticles containing lipophilic or amphiphilic drugs by using ethyl stearate as the core-fat.

Abstract translation: 本发明涉及由非甘油三酯油和由甘油三酯或硬脂酸乙酯组成的固体脂质纳米粒（SLN）组成的水包油脂质乳剂，其用作基因转染剂和药物递送系统及其制备方法。 本发明还涉及通过使用脂质乳剂和固体脂质纳米粒子将基因或药物有效地转移到细胞中的方法。 本发明还涉及通过使用角鲨烯或角鲨烷作为核心油来制备含有亲脂性或两亲性药物的脂质乳剂的方法。 本发明还涉及通过使用硬脂酸乙酯作为核心脂肪来制备含有亲脂性或两亲性药物的固体脂质纳米粒子的方法。

公开(公告)号：KR1020020066778A

公开(公告)日：2002-08-21

申请号：KR1020010007125

申请日：2001-02-13

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  권익찬 ,  정혜선

IPC: A61K47/44

CPC classification number: A61K9/0014 ,  A61K9/0019 ,  A61K47/44

Abstract: PURPOSE: A process of preparing the subject liquid or powder formulation is provided by adding an aqueous solution of poorly absorbable material in the body such as peptide, protein or water-soluble polymer to a mixture of monoglyceride, emulsifiers and organic solvents. Whereby, the formulation can be easily dispersed without addition of mechanical force and does not generate phase separation, hydrolysis and oxidation even when stored for long period time. CONSTITUTION: A composition to promote absorption of a poorly absorbable material in body comprises 9 to 90% by weight of one or more monoglycerides, 0.01 to 90% by weight of one or more emulsifiers, 0.01 to 10% by weight of an aqueous solution having solubility to a poorly absorbable material in body, 0 to 90.9% by weight of an organic solvent and 0 to 5% by weight of an additive. Also it is prepared by dissolving the monoglycerides and emulsifiers in the organic solvent containing the aqueous solution having solubility to a poorly absorbable material in body and removing a volatile organic solvent from the obtained viscose composition. The formulation contains 1 to 99% by weight of the composition and 1 to 99% by weight of an organic solvent.

Abstract translation: 目的：制备本发明液体或粉末制剂的方法是通过将不良吸收材料的水溶液（例如肽，蛋白质或水溶性聚合物）加入到单酸甘油酯，乳化剂和有机溶剂的混合物中来提供的。 因此，即使长期储存，配方也可以容易地分散而不增加机械力，并且不会产生相分离，水解和氧化。 构成：促进吸收体内吸收不良物质的组合物包含9至90重量％的一种或多种甘油单酯，0.01至90重量％的一种或多种乳化剂，0.01至10重量％的含有 对体内难以吸收的材料的溶解度，0-90.9重量％的有机溶剂和0-5重量％的添加剂。 另外，通过将单酸甘油酯和乳化剂溶解在含有对体内吸收不良的材料具有溶解性的水溶液的有机溶剂中，并从得到的粘胶组合物中除去挥发性有机溶剂。 该制剂含有1至99重量％的组合物和1至99重量％的有机溶剂。

公开(公告)号：KR1020020033665A

公开(公告)日：2002-05-07

申请号：KR1020020005448

申请日：2002-01-30

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  권익찬 ,  정혜선

IPC: A61K9/10

CPC classification number: A61K9/5123 ,  A61K9/1075 ,  A61K9/1272 ,  A61K48/00 ,  C12N15/88

Abstract: PURPOSE: Provided are a solid lipid nanoparticle as a gene or drug carrier, a formulation, and a method for preparing the same, thereby effectively transferring DNAs, bioprobes and drugs to the target cells. CONSTITUTION: The solid lipid nanoparticle comprises: a) at least one solid lipid nanoparticle material selected from C12-18 triglyceride or ethyl stearate, 2-30%; b) at least one kind of emulsifier including cationic surfactants, 0.01-20%; and c) the balance of water.

Abstract translation: 目的：提供作为基因或药物载体的固体脂质纳米颗粒，制剂及其制备方法，从而有效地将DNA，生物探针和药物转移至靶细胞。 构成：固体脂质纳米颗粒包含：a）至少一种选自C12-18甘油三酸酯或硬脂酸乙酯的固体脂质纳米颗粒材料，2-30％; b）至少一种乳化剂，包括阳离子表面活性剂，0.01-20％; 和c）水的平衡。