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公开(公告)号：KR1020080046796A
公开(公告)日：2008-05-28
申请号：KR1020060116233
申请日：2006-11-23
Abstract: A 3-hydroxybutyrate-co-3-hydroxypropionate-co-lactate terpolymer, and a method for preparing the terpolymer are provided to use the terpolymer as a biodegradable polymer capable of replacing synthetic plastics and being applied for medical use. A 3-hydroxybutyrate-co-3-hydroxypropionate-co-lactate terpolymer comprises lactate, 3-hydroxybutyrate and 3-hydroxypropionate as a repeating unit. The preparation method comprises the step of cultivating or culturing the cell or plant having the gene of an enzyme which converts lactate into lactyl-CoA and converts 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA and the gene of a polyhydroxyalkanoate(PHA) synthetase.
Abstract translation: 提供3-羟基丁酸酯 - 共-3-羟基丙酸酯 - 共乳酸三元共聚物和制备三元共聚物的方法，以使三元共聚物作为可替代合成塑料并用于医疗用途的可生物降解的聚合物。 3-羟基丁酸酯 - 共-3-羟基丙酸酯 - 共乳酸三元共聚物包含乳酸盐，3-羟基丁酸酯和3-羟基丙酸酯作为重复单元。 制备方法包括培养或培养具有将乳酸转化为乳酰辅酶A的酶的基因的细胞或植物，并将3-羟基链烷酸酯转化为3-羟基烷酰基-CoA和聚羟基链烷酸酯（PHA）合成酶的基因。
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62.发明公开
公开(公告)号：KR1020080045909A
公开(公告)日：2008-05-26
申请号：KR1020060115163
申请日：2006-11-21
Abstract: A 3-hydroxybutyrate-co-MCL 3-hydroxyalkanoate-co-lactate terpolymer, and a method for preparing the terpolymer are provided to obtain a biodegradable polymer capable of replacing synthetic plastics. A 3-hydroxybutyrate-co-MCL 3-hydroxyalkanoate-co-lactate terpolymer comprises lactate, 3-hydroxybutyrate and 3-hydroxyalkanoate of MCL(Middle Chain Length) as a repeating unit. The preparation method comprises the step of cultivating or culturing the cell or plant having the gene of an enzyme which converts lactate into lactyl-CoA and converts 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA and the gene of a polyhydroxyalkanoate(PHA) synthetase.
Abstract translation: 提供3-羟基丁酸酯 - 共-MCL 3-羟基链烷酸酯 - 共乳酸三元共聚物，以及制备三元共聚物的方法，以获得能够替代合成塑料的生物可降解聚合物。 3-羟基丁酸酯 - 共-MCL 3-羟基链烷酸酯 - 共乳酸三元共聚物包括作为重复单元的MCL（中链长度）的乳酸盐，3-羟基丁酸酯和3-羟基链烷酸酯。 制备方法包括培养或培养具有将乳酸转化为乳酰辅酶A的酶的基因的细胞或植物，并将3-羟基链烷酸酯转化为3-羟基烷酰基-CoA和聚羟基链烷酸酯（PHA）合成酶的基因。
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63.发明公开
公开(公告)号：KR1020070096348A
公开(公告)日：2007-10-02
申请号：KR1020060026599
申请日：2006-03-23
Abstract: Mutants having highly producing ability of 1,4-butanediol and a method for producing 1,4-butanediol by using the same mutants are provided to mass produce 1,4-butanediol from microorganisms by inserting enzyme genes producing 1,4-butanediol from succinate and deleting a gene producing succinate from succinate semialdehyde, so that the 1,4-butandiol is importantly used in chemical industry. The mutants capable of producing a large quantity of 1,4-butanediol contain an inserted gene encoding an enzyme converting succinate into 4-hydroxybutyrate(4HB), an inserted gene encoding an enzyme converting the 4-hydroxybutyrate into 1,4-butanediol(1,4-BDO) and a deleted gene associated with conversion of succinate semialdehyde into succinate and include bacterium, yeast and fungus, wherein the gene encoding the enzyme converting succinate into 4-hydroxybutyrate is selected from Cat1(succinyl-CoA transferase) gene of SEQ ID NO:1, SucD(succinate semialdehyde dehydrogenase) gene SEQ ID NO:2, 4hbD(4-hydroxybutyrate dehydrogenase) gene SEQ ID NO:3 and GHB(4-hydroxybutyrate dehydrogenase) gene SEQ ID NO:4; the gene encoding the enzyme converting succinate into 4-hydroxybutyrate is Cat2(4-hydroxybutyrate-CoA transferase) gene of SEQ ID NO:5 or the gene encoding alcohol dehydrogenase reducing 4HB-CoA of SEQ ID NO:6 or SEQ ID NO:7; and the gene associated with conversion of succinate semialdehyde into succinate is GabD(succinic semialdehyde dehydrogenase) gene of SEQ ID NO:8.
Abstract translation: 提供了具有高生产能力的1,4-丁二醇的突变体和通过使用相同的突变体生产1,4-丁二醇的方法，通过从琥珀酸中插入产生1,4-丁二醇的酶基因来从微生物中大量生产1,4-丁二醇 并从琥珀酸半醛中缺失产生琥珀酸的基因，使1,4-丁二醇在化学工业中重要使用。 能够产生大量1,4-丁二醇的突变体含有编码将琥珀酸酯转化为4-羟基丁酸酯（4HB）的插入基因，该基因编码将4-羟基丁酸转化为1,4-丁二醇的酶（1 ，4-BDO）和与琥珀酸盐半醛转化为琥珀酸相关的缺失基因，包括细菌，酵母和真菌，其中编码将琥珀酸酯转化为4-羟基丁酸酯的酶的基因选自SEQ ID（succinyl-CoA转移酶）基因 ID NO：1，SucD（琥珀酸半醛脱氢酶）基因SEQ ID NO：2,4hbD（4-羟基丁酸脱氢酶）基因SEQ ID NO：3和GHB（4-羟基丁酸脱氢酶）基因SEQ ID NO：4; 将琥珀酸酯转化为4-羟基丁酸的基因是SEQ ID NO：5的Cat2（4-羟基丁酸酯-CoA转移酶）基因或编码SEQ ID NO：6或SEQ ID NO：7的醇脱氢酶还原型4HB-CoA的基因 ; 与琥珀酸半醛转化为琥珀酸相关的基因是SEQ ID NO：8的GabD（琥珀酸半醛脱氢酶）基因。
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公开(公告)号：KR100690948B1
公开(公告)日：2007-03-09
申请号：KR1020040044881
申请日：2004-06-17
Applicant: 한국과학기술원
CPC classification number: C12N15/70
Abstract: 본 발명은 대장균에서 유래한 세포 외막 단백질 (FadL)을 세포표면 발현모체로 사용하여 목적단백질이나 펩타이드를 세포표면에 효율적으로 발현시킬 수 있는 발현벡터, 상기 발현벡터로 형질전환된 미생물, 상기 형질전환 미생물을 배양하여 목적 단백질을 세포표면에 안정적으로 다량 발현시키는 방법 및 상기 표면발현된 목적 단백질을 이용하는 것을 특징으로 하는 단백질 어레이의 제조방법, 항체의 제조방법, 생물전환방법 및 목적단백질의 개량방법에 관한 것이다.
본 발명에 의하면, 세포 외막에 목적단백질을 정상적인 기능을 가진 상태로 발현시킬 수 있으므로, 삽입되는 목적유전자에 따라, 재조합 생백신, 여러 펩타이드나 항체의 선별, 중금속 제거 또는 폐수처리에 응용할 수 있는 전세포 흡착제, 전세포 생물전환 등에 유용하게 사용할 수 있다.
세포외막 단백질, 표면발현, FadL, 개량, 단백질 어레이, 항체, 생물전환-
65.发明公开폴리락테이트 또는 그 공중합체 생성능을 가지는 세포 또는식물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법 有权具有聚合物或其共聚物的生产能力的电池或植物及其制备聚乙烯或其共聚物的方法
公开(公告)号：KR1020060121555A
公开(公告)日：2006-11-29
申请号：KR1020050043798
申请日：2005-05-24
CPC classification number: C12P7/625 , C12N9/10 , C12N9/1025 , C12N9/93 , C12N15/70 , C12N15/74 , C12N15/8242 , C12N15/8243 , C12N2500/35 , Y02P20/52
Abstract: Cells or plants capable of producing polylactate or its copolymers and a method for producing the polylactate or its copolymers by using the same cells or plants are provided to produce polylactate and various polyesters containing hydroxyalkanoate as a monomer from cells or plants. The cells or plants capable of producing polylactate or poly(hydroxyalkanoate-co-lactate) contain both of the gene encoding enzyme capable of converting lactate into lactyl-CoA and PHA(polyhydroxyalkanoates) synthetase gene, wherein the gene encoding enzyme capable of converting lactate into lactyl-CoA is propionyl-CoA transferase gene(pct); the PHA synthetase gene using lactyl-CoA as a substrate is phaC or phaRBC; and the cell is Escherichia coli. The method for producing polylactate or its copolymers comprises the steps of: culturing a cell in medium containing lactate and various hydroxyalkanoate as carbon sources; and collecting polylactate or hydroxyalkanoate-lactate copolymer(poly(hydroxyalkanoate-co-lactate)) from the cultured cell.
Abstract translation: 提供能够生产聚乳酸或其共聚物的细胞或植物，以及通过使用相同的细胞或植物制备聚乳酸或其共聚物的方法，以生产含有羟基链烷酸酯作为单体或多种植物的聚羟基链烷酸酯的聚乳酸酯和各种聚酯。 能够产生聚乳酸或聚（羟基链烷酸酯 - 共乳酸）的细胞或植物含有能够将乳酸盐转化为乳酰基-CoA的基因编码酶和PHA（聚羟基链烷酸酯）合成酶基因的两者，其中能够将乳酸盐转化为 乳酰辅酶A是丙酰辅酶A转移酶基因（pct）; 使用乳酰辅酶A作为底物的PHA合成酶基因是phaC或phaRBC; 细胞是大肠杆菌。 生产聚乳酸或其共聚物的方法包括以下步骤：在含有乳酸盐和各种羟基链烷酸酯的培养基中培养细胞作为碳源; 并从培养的细胞中收集聚乳酸或羟基链烷酸酯 - 乳酸共聚物（聚（羟基链烷酸酯 - 共乳酸））。
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Patent Agency Ranking66.发明授权
公开(公告)号：KR100530988B1
公开(公告)日：2005-11-28
申请号：KR1020030015965
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C12N1/21
CPC classification number: C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
Abstract: 본 발명은 봉입체 결합 단백질을 코딩하는 유전자(
ibpA 및/또는
ibpB )를 결핍시키거나 증폭시켜 목적단백질을 제조하는 방법에 관한 것이다. 본 발명은 지금까지 목적 단백질 생산에 미치는 영향이 보고되지 않아왔던 대장균 유래의 봉입체 결합 단백질을 코딩하는 유전자(
ibpA 및/또는
ibpB )를 이용한 목적 단백질 제조방법 두 가지를 제공한다. 첫째,
ibpA 및/또는
ibpB 가 결핍된 박테리아를 이용하여 목적 단백질의 분비·생산 및 활성을 향상시키는 방법을 제공한다. 둘째,
ibpA 및/또는
ibpB 가 증폭된 박테리아를 이용하여 세포질 내에 생산되는 목적단백질의 생산성 향상과 아울러 목적단백질을 수용성 형태에서 불용성 봉입체로 생산하는 방법을 제공한다.-
公开(公告)号：KR100523211B1
公开(公告)日：2005-10-24
申请号：KR1020030005730
申请日：2003-01-29
Applicant: 한국과학기술원
IPC: G01N33/543
Abstract: 본 발명은 폴리하이드록시알칸산 분해효소 기질결합 도메인의 폴리하이드록시알칸산에 대한 기질특이성을 이용하여, 목적 단백질을 기판에 위치특이적으로 고정시킨 단백질 칩 및 전기 단백질 칩을 이용한 단백질간 반응검출 방법에 관한 것이다. 본 발명에 의하면, 목적 단백질을 기판 위에 위치-특이적으로 고정시켜 비특이적인 단백질 결합을 억제할 수 있으므로, 단백질 칩 제작시의 봉쇄공정과 복잡한 정제공정을 필요로 하지 않아 생산성과 경제성의 측면에서 큰 향상효과를 기대할 수 있으며, 본 발명의 단백질 칩을 이용하면 단백질간 상호반응을 보다 특이적이고 효율적으로 수행할 수 있으므로, 신약개발의 기초연구에 크게 기여할 수 있을 것이다.
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68.发明授权
公开(公告)号：KR100516389B1
公开(公告)日：2005-09-23
申请号：KR1020030062756
申请日：2003-09-08
Applicant: 한국과학기술원
IPC: C07K14/00
CPC classification number: C07K14/195 , C07K14/00
Abstract: 본 발명은 작은 열 충격 단백질(sHSPs)을 함유하는 단백질 분해 방지용 조성물 및 이차원 전기영동용 조성물에 관한 것이다. 또한 본 발명은 sHSPs를 이용하는 것을 특징으로 하는 개선된 이차원 전기영동(two-dimensional gel electrophoresis) 방법에 관한 것이다. 본 발명에 따르면, 이차원 전기영동시 단백질 스팟(spot)이 감소되는 것을 방지하여 훨씬 많은 단백질 스팟을 포함한 이차원 전기영동 사진을 제공할 수 있다.
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公开(公告)号：KR1020040092044A
公开(公告)日：2004-11-03
申请号：KR1020030025863
申请日：2003-04-23
Applicant: 한국과학기술원
IPC: C12N15/11
CPC classification number: C12N9/93 , C08G63/06 , C12N9/88 , C12P7/625 , C12Y402/01017
Abstract: PURPOSE: A process for preparing polyhydroxyalkanoate using MaoC protein is provided, thereby preparing high quality polyhydroxyalkanoate having larger number of carbons in higher yield. CONSTITUTION: A MaoC gene encoding a protein providing a monomer required for synthesis of MCL-PHA(medium-chain-length polyhydroxyalkanoate) has the nucleotide sequence set forth in SEQ ID NO:2. A protein MaoC being expressed from the maoC gene and having enoyl-CoA hydratase activity has the amino acid sequence set forth in SEQ ID NO:1. A recombinant expression vector contains the maoC gene of SEQ ID NO:2. The process for preparing polyhydroxyalkanoate using MaoC protein comprises the steps of: (i) removing fadB gene from Escherichia coli; (ii) co-transforming the E. coli with both of the expression vector containing the maoC gene and an expression vector containing a PHA synthesizing gene phaC to give a transformant; and (iii) culturing the transformant in a medium containing C6-10 carbon source to give PHA consisting of monomers having C6-10 carbon source.
Abstract translation: 目的：提供使用MaoC蛋白制备聚羟基链烷酸酯的方法，从而以更高的产率制备具有较大碳数的高质量聚羟基链烷酸酯。 构成：编码提供合成MCL-PHA（中链长度聚羟基链烷酸酯）所需的单体的蛋白质的MaoC基因具有SEQ ID NO：2所示的核苷酸序列。 由maoC基因表达并具有烯酰辅酶A水合酶活性的蛋白质MaoC具有SEQ ID NO：1所示的氨基酸序列。 重组表达载体含有SEQ ID NO：2的maoC基因。 使用MaoC蛋白制备聚羟基链烷酸酯的方法包括以下步骤：（i）从大肠杆菌中除去fadB基因; （ii）用含有maoC基因的表达载体和含有PHA合成基因phaC的表达载体共转化大肠杆菌，得到转化体; 和（iii）在含有C6-10碳源的培养基中培养转化体以得到由具有C6-10碳源的单体组成的PHA。
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公开(公告)号：KR100447535B1
公开(公告)日：2004-09-08
申请号：KR1020020010324
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
Abstract: PURPOSE: A recombinant bacterial system for producing P(3HB-co-3HA) is provided, thereby mass-producing poly(3-hydroxyalkanoate) (PHA) containing a large amount of 3HB having similar properties to the conventional synthetic polymer. CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, beta-ketothiolase synthesizing gene phaA, and NADPH dependent acetoacetyl-CoA reductase synthesizing gene phaB is provided, wherein the recombinant vector is p104613C2ReABstb. A transformant transformed with the recombinant expression vector p104613C2ReABstb is provided, wherein the transformant is Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb). A method for producing P(3HB-co-3HA) comprises culturing a transformant selected from Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb) in a medium containing fatty acid or both fatty acid and carbohydrate.
Abstract translation: 目的：提供一种用于生产P（3HB-共-3HA）的重组细菌系统，由此批量生产含有大量3HB的聚（3-羟基链烷酸酯）（PHA），其具有与常规合成聚合物相似的性质。 构成：提供了含有PHA合成基因phaC，β-酮硫解酶合成基因phaA和NADPH依赖性乙酰乙酰-CoA还原酶合成基因phaB的重组表达载体，其中重组载体是p104613C2ReABstb。 提供了用重组表达载体p104613C2ReABstb转化的转化体，其中转化体是大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）。 一种P（3HB-co-3HA）的制造方法，其特征在于，在含有脂肪酸或脂肪酸和脂肪酸的培养基中培养选自大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）的转化体 糖类。
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