-
公开(公告)号：KR1020100111073A
公开(公告)日：2010-10-14
申请号：KR1020090029452
申请日：2009-04-06
Applicant: 한국과학기술원
CPC classification number: B63C9/082 , B63B2728/00 , B63B2729/00 , B63C9/18 , B63C2009/0041
Abstract: PURPOSE: A water rescue apparatus for easily using a general person without specialized knowledge is provided to rescue the people in a short time and increase the portability of the water rescue apparatus. CONSTITUTION: A rescue someone in water apparatus(100) includes a case(10), a valve(40) and a tube. The case generates the gas in response to the water. The case is formed so that an inlet port and a vent be connected to inside. The valve is combined in the inlet port of the case so that the inlet port of the case be closed with opening. The valve is operated with the water pressure so that the inlet port is opened and the inside of the case be connected to outside. The inlet port connected to vent is formed.
Abstract translation: 目的：提供一种在没有专业知识的情况下轻松使用一般人员的水上救援设备，以便在短时间内救人，增加水援救设备的便携性。 构成：救援人员在水设备（100）中包括一个壳体（10），一个阀门（40）和一个管子。 这种情况会响应于水而产生气体。 壳体形成为使得入口和通风口连接到内部。 阀门组合在壳体的入口端口，使得壳体的入口与开口闭合。 阀门以水压进行操作，使得进气口打开，外壳内部连接。 连接到通风口的入口形成。
-
公开(公告)号：KR101091823B1
公开(公告)日：2011-12-12
申请号：KR1020090029452
申请日：2009-04-06
Applicant: 한국과학기술원
Abstract: 본발명은크기가작아휴대하기편리하고멀리까지도쉽게던질수 있으며전문지식이없는일반인도쉽게사용할수 있을뿐만아니라구조자가물에들어가지않고서도조난자를구조할수 있어패닉상태에있는조난자에의한구조자의인명피해도미연에방지할수 있도록구조가개선된수상인명구조장치에관한것이다. 본발명에따른수상인명구조장치는유입구및 배출구가내부와연결되게형성된케이스; 케이스의내부에배치되고, 중공형상으로이루어지며, 관통공이외측면에형성된제1실린더부재; 케이스의내부에배치되고, 제1실린더부재가삽입되는중공형상으로이루어지며, 관통공이외측면에형성되고, 물과반응하여기체를생성하는반응물질이제1실린더부재와의사이에배치되어있는제2실린더부재; 케이스의유입구가개방및 폐쇄되도록케이스의유입구에결합되며, 유입구가개방되어케이스의내부가외부와연결되도록수압에의해작동하는밸브; 및케이스내부에서생성되는기체가케이스의배출구를통해내부로유입되도록배출구와연결되는유입구가형성되며, 유입된기체에의해부유가능한튜브;를구비한다.
-
3.发明公开작은 열 충격 단백질을 함유하는 단백질 분해 방지용조성물 및 이를 이용한 이차원 전기영동법 失效用于保护包含小热休克蛋白（SHSPS）的蛋白质降解的组合物和使用SHSPS的两维凝胶电泳方法
公开(公告)号：KR1020050025819A
公开(公告)日：2005-03-14
申请号：KR1020030062756
申请日：2003-09-08
Applicant: 한국과학기술원
IPC: C07K14/00
CPC classification number: C07K14/195 , C07K14/00
Abstract: A composition for protecting proteins degradation comprising small heat shock proteins(sHSPs) and a method of two-dimensional gel electrophoresis using the same sHSPs are provided, thereby inhibiting reduction of protein spots when carrying out electrophoresis, so that a larger number of spots can be detected by the two-dimensional gel electrophoresis. The composition for protecting proteins degradation comprises the effective amount of small heat shock proteins(sHSPs), wherein the small heat shock proteins(sHSPs) has small molecular weight about 15 to 30 kDa. The method of two-dimensional gel electrophoresis using the sHSPs comprises the steps of: adding sHSPs into a protein mixture; and subjecting the protein mixture to two-dimensional gel electrophoresis, wherein the sHSP is one or more selected from IbpA, IbpB and IbpAB derived from E. coli, IbpA derived from Pseudomonas sp. and HSP26 derived from Saccharomyces cerevisiae; and the protein mixture is the total proteins of a certain cell of prokaryotes such as E. coli or Pseudomonas sp. or eukaryotes such as human.
Abstract translation: 提供了一种用于保护包含小型热休克蛋白（sHSP）的蛋白质降解的组合物和使用相同sHSP的二维凝胶电泳方法，从而抑制了进行电泳时蛋白质斑点的减少，从而可以获得更多的斑点 通过二维凝胶电泳检测。 用于保护蛋白质降解的组合物包含有效量的小热休克蛋白（sHSP），其中小热休克蛋白（sHSP）具有约15至30kDa的小分子量。 使用sHSP的二维凝胶电泳的方法包括以下步骤：将sHSP加入到蛋白质混合物中; 并将所述蛋白质混合物进行二维凝胶电泳，其中所述sHSP是选自源自大肠杆菌的IbpA，IbpB和IbpAB中的一种或多种，源自假单胞菌属的IbpA。 和来自酿酒酵母的HSP26; 并且蛋白质混合物是原核生物的某些细胞如大肠杆菌或假单胞菌属的总蛋白质。 或真核生物如人类。
-
公开(公告)号：KR1020040072992A
公开(公告)日：2004-08-19
申请号：KR1020030008689
申请日：2003-02-12
Applicant: 한국과학기술원
IPC: C07K14/575
CPC classification number: C07K14/5434 , C07K14/5759 , C12N15/70 , C12P21/02
Abstract: PURPOSE: A process for preparing a serine-rich protein employing cysteine synthetase(cysK) is provided, thereby significantly reducing the time required for maximum protein production, so that the preparation yield of serine-rich protein can be improved. CONSTITUTION: The process for preparing a foreign protein comprises the steps of: simultaneously transforming a microorganism with a recombinant vector containing gene cysK and a vector containing a gene encoding a foreign protein such as serine-rich protein; culturing the transformed microorganism; and collecting the foreign protein from the cultured medium, wherein the serine-rich protein is leptin or interleukin 12 β chain.
Abstract translation: 目的：提供使用半胱氨酸合成酶（cysK）制备富含丝氨酸的蛋白质的方法，从而显着减少最大蛋白质产生所需的时间，从而可以提高富含丝氨酸蛋白质的制备产率。 构成：制备外源蛋白质的方法包括以下步骤：用含有基因cysK的重组载体和含有编码外源蛋白的基因如富含丝氨酸的蛋白质的载体同时转化微生物; 培养转化的微生物; 并从培养基中收集外源蛋白质，其中富含丝氨酸的蛋白质是瘦素或白细胞介素12β链。
-
公开(公告)号：KR1020030070789A
公开(公告)日：2003-09-02
申请号：KR1020020010324
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
CPC classification number: C12N15/70 , C12P7/625 , C12Y101/01036 , C12Y203/01016
Abstract: PURPOSE: A recombinant bacterial system for producing P(3HB-co-3HA) is provided, thereby mass-producing poly(3-hydroxyalkanoate) (PHA) containing a large amount of 3HB having similar properties to the conventional synthetic polymer. CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, beta-ketothiolase synthesizing gene phaA, and NADPH dependent acetoacetyl-CoA reductase synthesizing gene phaB is provided, wherein the recombinant vector is p104613C2ReABstb. A transformant transformed with the recombinant expression vector p104613C2ReABstb is provided, wherein the transformant is Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb). A method for producing P(3HB-co-3HA) comprises culturing a transformant selected from Escherichia coli WA101(p104613C2ReABstb), Escherichia coli WB101(p104613C2ReABstb), or Escherichia coli WAB101(p104613C2ReABstb) in a medium containing fatty acid or both fatty acid and carbohydrate.
Abstract translation: 目的：提供一种用于生产P（3HB-co-3HA）的重组细菌系统，从而大量生产含有大量与常规合成聚合物具有相似性质的3HB的聚（3-羟基链烷酸酯）（PHA）。 构成：提供了含有PHA合成基因phaC，β-酮硫解酶合成基因phaA和NADPH依赖性乙酰乙酰辅酶A还原酶合成基因phaB的重组表达载体，其中重组载体为p104613C2ReABstb。 提供了用重组表达载体p104613C2ReABstb转化的转化体，其中转化体是大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）。 制备P（3HB-co-3HA）的方法包括在含有脂肪酸或脂肪酸两者的介质中培养选自大肠杆菌WA101（p104613C2ReABstb），大肠杆菌WB101（p104613C2ReABstb）或大肠杆菌WAB101（p104613C2ReABstb）的转化体， 糖类。
-
Patent Agency Ranking
公开(公告)号：KR1020050079582A
公开(公告)日：2005-08-10
申请号：KR1020040008077
申请日：2004-02-06
Applicant: 한국과학기술원
CPC classification number: C07K1/16 , C07K14/21 , C07K14/245 , C07K14/395 , C07K2319/00 , C12P21/00
-
公开(公告)号：KR100489500B1
公开(公告)日：2005-05-16
申请号：KR1020030008689
申请日：2003-02-12
Applicant: 한국과학기술원
IPC: C07K14/575
CPC classification number: C07K14/5434 , C07K14/5759 , C12N15/70 , C12P21/02
Abstract: 본 발명은 세린(serine) 부유 외래 단백질의 유전자를 포함하는 발현벡터 및 시스테인 합성효소 유전자를 포함하는 발현벡터로 형질전환된 미생물을 배양하고, 이로부터 세린 풍부 외래 단백질을 제조하는 방법에 관한 것이다. 본 발명의 시스테인 합성효소 유전자를 이용한 세린 풍부 단백질의 제조방법은
cysK 유전자를 포함하는 재조합 벡터와 외래 단백질을 코딩하는 유전자를 함유하는 벡터로 동시에 형질전환된, 재조합 미생물을 배양하고, 이로부터 외래 단백질을 수득하는 단계를 포함한다. 본 발명에 의하면, 재조합 대장균을 이용한 세린 풍부 외래 단백질의 생산시, 최대 단백질 생산량에 이르는 시간을 크게 단축시킬 수 있으므로, 세린 풍부 외래 단백질의 제조수율을 향상시키는데 널리 활용될 수 있을 것이다.-
8.发明公开
公开(公告)号：KR1020040081237A
公开(公告)日：2004-09-21
申请号：KR1020030015965
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C12N1/21
CPC classification number: C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
Abstract: PURPOSE: A method for producing target proteins by deleting or amplifying ibpA and/or ibpB gene encoding inclusion body-associated proteins is provided, thereby inducing secretion of water-soluble protein having its activity, and improving productivity of target proteins which are produced in a type of inclusion body within cytoplasm of a bacterium having the amplified ibpA and/or ibpB gene. CONSTITUTION: The method for producing target proteins by deleting the ibpA and/or ibpB gene encoding inclusion body-associated proteins comprises culturing the ibpA and/or ibpB gene deleted bacterium and a gene encoding the target gene, wherein the ibpA and/or ibpB gene deleted bacterium is Escherichia coli WIB101; the bacterium further contains secretion signal sequence; and the target protein is leptin or alkaline phosphatase. The method for producing target proteins by amplifying the ibpA and/or ibpB gene encoding inclusion body-associated proteins comprises culturing the ibpA and/or ibpB gene amplified bacterium and a gene encoding the target gene, wherein the ibpA and/or ibpB gene deleted bacterium is produced by using plasmid pACTacIbpAB, pACIbpAB, pACTacIbpA or pACTacIbpB which can amplify the ibpA and/or ibpB gene; the ibpA and/or ibpB gene deleted bacterium is Escherichia coli; the target proteins are produced within a water-insoluble inclusion body; the target proteins are accumulated in cytoplasm of the bacterium; and the target protein is insulin-like growth factor-I, interleukin 12 beta chain, interferon-gamma or green fluorescent protein(GFP).
Abstract translation: 目的：提供通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法，从而诱导具有其活性的水溶性蛋白质的分泌，并提高在 具有扩增的ibpA和/或ibpB基因的细菌的细胞质内的包涵体的类型。 构成：通过缺失编码包涵体相关蛋白质的ibpA和/或ibpB基因产生靶蛋白的方法包括培养ibpA和/或ibpB基因缺失的细菌和编码靶基因的基因，其中所述ibpA和/或ibpB基因 缺失的细菌是大肠杆菌WIB101; 该细菌还含有分泌信号序列; 靶蛋白是瘦蛋白或碱性磷酸酶。 通过扩增编码包涵体相关蛋白质的ibpA和/或ibpB基因来产生靶蛋白的方法包括培养ibpA和/或ibpB基因扩增的细菌和编码靶基因的基因，其中所述ibpA和/或ibpB基因缺失的细菌 是通过使用能够扩增ibpA和/或ibpB基因的质粒pACTacIbpAB，pACIbpAB，pACTacIbpA或pACTacIbpB产生的; ibpA和/或ibpB基因缺失的细菌是大肠杆菌; 靶蛋白在水不溶性包涵体内产生; 目标蛋白质在细菌的细胞质中积累; 靶蛋白是胰岛素样生长因子-I，白介素12β链，干扰素-γ或绿色荧光蛋白（GFP）。
-
公开(公告)号：KR100655493B1
公开(公告)日：2006-12-07
申请号：KR1020040008077
申请日：2004-02-06
Applicant: 한국과학기술원
Abstract: 본 발명은 프로테아제에 의한 목적단백질의 분해를 방지하기 위하여 유효량의 작은 열 충격 단백질 (sHSPs)을 첨가하는 것을 특징으로 하는 목적단백질의 분리·정제방법, 목적단백질의 제조방법 및 전세포(whole cell) 효소 또는 부분정제된 효소를 이용한 효소반응 방법에 관한 것이다.
본 발명에 따르면, 목적단백질의 제조를 위한 배양공정 및 목적단백질의 분리·정제공정에 sHSPs를 첨가할 경우, 프로테아제에 의한 단백질 손실을 방지함으로써 높은 수율로 목적단백질을 수득할 수 있다. 또한, 전세포(whole cell) 효소 또는 부분정제된 효소를 이용하는 반응공정에 sHSPs를 첨가할 경우, 프로테아제에 의한 효소의 손실을 방지함으로써 효소반응의 수율을 높일 수 있다.
sHSP, 단백질, 분리, 정제, 효소 반응-
10.发明授权
公开(公告)号：KR100530988B1
公开(公告)日：2005-11-28
申请号：KR1020030015965
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C12N1/21
CPC classification number: C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
Abstract: 본 발명은 봉입체 결합 단백질을 코딩하는 유전자(
ibpA 및/또는
ibpB )를 결핍시키거나 증폭시켜 목적단백질을 제조하는 방법에 관한 것이다. 본 발명은 지금까지 목적 단백질 생산에 미치는 영향이 보고되지 않아왔던 대장균 유래의 봉입체 결합 단백질을 코딩하는 유전자(
ibpA 및/또는
ibpB )를 이용한 목적 단백질 제조방법 두 가지를 제공한다. 첫째,
ibpA 및/또는
ibpB 가 결핍된 박테리아를 이용하여 목적 단백질의 분비·생산 및 활성을 향상시키는 방법을 제공한다. 둘째,
ibpA 및/또는
ibpB 가 증폭된 박테리아를 이용하여 세포질 내에 생산되는 목적단백질의 생산성 향상과 아울러 목적단백질을 수용성 형태에서 불용성 봉입체로 생산하는 방법을 제공한다.
-
-
-
-
-
-
-
-
-
Search Results
12
records
(took 0.022 seconds)
