公开(公告)号：WO1985005458A1

公开(公告)日：1985-12-05

申请号：PCT/US1985000914

申请日：1985-05-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KOLB, Alfred, J. ,  HORROCKS, Donald, L.

IPC: G01T01/204

CPC classification number: G01T1/2045

Abstract: A single sample quench determination method including generating a pulse height distribution spectrum for the sample in the presence of a standard source, measuring a pulse height value corresponding to a unique point on the spectrum, counting all pulses produced by a radionuclide within the sample, counting pulses produced by the radionuclide in the sample within a calibration window, optically varying the quench of the single sample and repeating such steps, correlating normalized pulse height values to the counts for all pulses produced by the radionuclide within the sample at varying degrees of quench to determine disintegrations per minute within the single sample, determining efficiency for the pulse counts measured within the calibration window, and relating such efficiencies to an indication of quench to thereby provide a quench relationship from a single sample.

Abstract translation: 单样品淬火确定方法，包括在存在标准源的情况下产生样品的脉冲高度分布谱，测量对应于光谱上的唯一点的脉冲高度值，对由样品内的放射性核素产生的所有脉冲进行计数，计数 在校准窗口内由样品中的放射性核素产生的脉冲，光学地改变单个样品的骤冷并重复这样的步骤，将标准化的脉冲高度值与由样品内的放射性核素在不同程度的骤冷至 确定单个样品内每分钟的崩解，确定在校准窗口内测量的脉冲计数的效率，并将这些效率与骤冷指示相关联，从而提供来自单个样品的骤冷关系。

公开(公告)号：WO1985005157A1

公开(公告)日：1985-11-21

申请号：PCT/US1985000443

申请日：1985-03-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GIEBELER, Robert, Henry, Jr.

IPC: F16C33/66

CPC classification number: B04B9/12 ,  F16C33/6666 ,  F16C33/6677 ,  F16N7/366 ,  F16N2210/22 ,  H02K5/1732 ,  H02K9/19 ,  Y10S384/90

Abstract: A system for cooling and lubricating the drive motor of a centrifuge. The shaft (16) of the motor (10) includes an internal passage (40) with an inlet (48) having a size and taper sufficient to provide the desired rate of cooling oil flow through the motor. A lower bearing proportioning arrangement (40) applied a high percentage of a small proportion of the total oil flow to the lower bearing (20). An upper bearing proportioning arrangement (50) applies a low percentage of a large proportion of the total oil flow to the upper bearing (18). Together these features assure a high degree of motor cooling without overlubricating the bearings.

公开(公告)号：WO1985004252A1

公开(公告)日：1985-09-26

申请号：PCT/US1985000408

申请日：1985-03-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ULFELDER, Kathi, J. ,  GURSKE, William, A.

IPC: G01N27/26

CPC classification number: G01N27/44747

Abstract: An electrophoretic procedure of the type comprising (a) applying at least one sample to a gel; (b) electrophoresing the gel of step (a); (c) fixing the electrophoresed gel of step (b); and (d) drying the fixed gel of step (c). The electrophoretic procedure is characterized in that a novel electrophoretic gel is employed therein. The novel electrophoretic gel is of the type comprising (a) at least one marine hydrocolloid processed from the class Rodophycea; and (b) a buffer. The electrophoretic gel is characterized in that the buffer (i) is selected from a group consisting of adipic acid, glutaric acid, itaconic acid, maleic acid, malic acid, malonic acid, succinic acid, succinamic acid, and tricarbalyllic acid and (ii) is present in a concentration of about 0.05 to about 0.09 M.

Abstract translation: 该类型的电泳方法包括（a）将至少一种样品施加到凝胶上; （b）电泳步骤（a）的凝胶; （c）固定步骤（b）的电泳凝胶; 和（d）干燥步骤（c）的固定凝胶。 电泳方法的特征在于其中使用新的电泳凝胶。 该新颖的电泳凝胶是由（a）至少一种从枸杞类处理的海洋水解胶体的类型; 和（b）缓冲器。 电泳凝胶的特征在于缓冲液（i）选自己二酸，戊二酸，衣康酸，马来酸，苹果酸，丙二酸，琥珀酸，琥珀酰胺酸和三芳烃酸组成的组，（ii） 以约0.05至约0.09M的浓度存在。

公开(公告)号：WO1984003558A1

公开(公告)日：1984-09-13

申请号：PCT/US1984000362

申请日：1984-03-05

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WIJNTJES, Geert ,  HERCHER, Michael

IPC: G01B09/02

CPC classification number: G01J3/4535 ,  G01J3/0243

Abstract: An improved mirror scan control for driving a movable mirror (14) in an interferometer with a constant scan velocity comprises a closed loop servo control which provides constant velocity mirror scan in response to a phase comparison of a signal derived from the beat frequency of a two frequency laser beam (16) passing through the interferometer and a selected frequency reference signal, wherein the difference in frequencies or beat frequency of the two frequency laser beam is stabilized at a specified difference in frequencies. The mirror scan control employs a phase lock control loop which locks the frequency of the referenced signal with the frequency of the signal derived from the laser beam to provide precise mirror velocity control.

Abstract translation: 用于以恒定扫描速度驱动干涉仪中的可移动反射镜（14）的改进的反射镜扫描控制包括闭环伺服控制器，其响应于来自二次拍子频率的信号的相位比较而提供恒定速度镜像扫描 通过干涉仪的频率激光束（16）和选定的频率参考信号，其中两个频率激光束的频率或拍频的差异被稳定在特定的频率差。 镜像扫描控制采用锁相控制环路，其以参考信号的频率与从激光束得到的信号的频率进行锁定，以提供精确的镜像速度控制。

公开(公告)号：WO1984002776A1

公开(公告)日：1984-07-19

申请号：PCT/US1984000031

申请日：1984-01-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OHMS, Jack, I. ,  PENHASI, Harry, A. ,  QADEER, Majid

IPC: G01N33/16

CPC classification number: B01J19/0046 ,  B01J2219/00283 ,  B01J2219/0049 ,  B01J2219/0059 ,  B01J2219/00725 ,  C40B40/10 ,  C40B60/14

Abstract: An improved apparatus for sequencing proteins and peptides in a reaction chamber using a spinning cup by successive coupling and cleavage reaction steps wherein the reaction chamber is always subjected to positive pressure. The improvement involves the elimination of the use of a vacuum in the chamber for drying or enhancing reagent or solvent transfer. Throughout the coupling and cleavage steps the reaction chamber is subjected to a positive pressure by the positive application of an inert gas through the reaction chamber for reagent and solvent transfer, drying and removal of waste.

Abstract translation: 一种用于使用旋转杯通过连续偶联和裂解反应步骤在反应室中测序蛋白质和肽的改进装置，其中反应室总是经受正压。 改进包括消除在室中使用真空来干燥或增强试剂或溶剂转移。 在整个偶联和裂解步骤中，通过惰性气体通过反应室进行反应和溶剂转移，干燥和除去废物，使反应室受到正压。

公开(公告)号：WO1984001032A1

公开(公告)日：1984-03-15

申请号：PCT/US1983001300

申请日：1983-08-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHU, Frank, R. ,  OLANDER, Glenn, J.

IPC: G01N33/72

CPC classification number: G01N33/728

Abstract: A method for assaying the direct bilirubin content of a sample comprising (a) mixing a sulfanilic acid solution comprising from about 0.02 to about 0.05 M sulfanilic acid and from about 0.02 to about 0.15 M hydrochloric acid with a sodium nitrite to thereby commence a diazotation process, the ratio of the sulfanilic acid solution to said sodium nitrate solution being preselected so as to yield a diazo solution comprising from about 0.0001 to about 0.001 M Diazo dye; (b) allowing the diazotation process to form the diazo solution for at least 15 seconds; (c) introducing an aliquot of the sample to be assayed into said diazo solution at a volume ratio of from about 1:100 to about 1:25 to thereby commence an azobilirubin process; and (d) measuring the instantaneous rate of azobilirubin formation at 545 nm in a time range of from about 12 to about 25 seconds after introduction of the aliquot of sample into the diazo solution.

Abstract translation: 一种用于测定样品的直接胆红素含量的方法，包括（a）将包含约0.02至约0.05M的对氨基苯磺酸和约0.02至约0.15M盐酸的对氨基苯磺酸溶液与亚硝酸钠混合，从而开始重氮化过程 预选氨磺酸溶液与所述硝酸钠溶液的比例，以产生包含约0.0001-约0.001M重氮染料的重氮溶液; （b）使重氮化过程形成至少15秒的重氮溶液; （c）将体积比为约1:100至约1:25的待分析样品的等分试样引入所述重氮溶液中，从而开始偶氮胆红素方法; 和（d）在将样品等分试样引入重氮溶液之后，测量在约54至约25秒的时间范围内的545nm处的偶氮胆红素形成的瞬时速率。

公开(公告)号：WO1984000978A1

公开(公告)日：1984-03-15

申请号：PCT/US1983001299

申请日：1983-08-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BECK, James, P. ,  LUNA, Carlos, E.

IPC: C12Q01/48

CPC classification number: C12Q1/48

Abstract: A substrate solution comprising (a) a substrate selected from a group consisting of salts of L- gamma -glutamyl-p-nitroanilide and L- gamma -glutamyl-3-carboxy-p-nitroanilide; and (b) a polyol selected from a group consisting of alkyl polyols containing 2 to 10 carbon atoms and 2 to 10 hydroxy groups and polyethylene glycol having an average molecular weight of from about 200 to about 600. Also, a kit for the determination of gamma -glutamyltransferase activity of the type comprising (a) a buffered medium comprising a buffer and a gamma -glutamyl residue acceptor; and (b) a substrate solution; the buffered medium and substrate solution being adapted such that preselected aliquots thereof can react in the presence of gamma -glutamyltransferase to produce an absorbing cleavage product. The kit is characterized in that the substrate solution is as described above. In, addition, a method for determining gamma -glutamyltransferase activity of the type comprising (a) contacting in any preselected order an aliquot of a buffered medium comprising a buffer and a gamma -glumtamyl residue acceptor, an aliquotof a substrate solution, and an aliquot of a sample to be assayed to thereby form a cleavage product; and (b) measuring the absorbance of said cleavage product. The method is characterized in that the substrate solution is as described above.

Abstract translation: 一种底物溶液，其包含（a）选自由L-γ-谷氨酰 - 对硝基苯胺和L-γ-谷氨酰基-3-羧基 - 对硝基苯胺的盐组成的组的底物; 和（b）选自含有2至10个碳原子的烷基多元醇和2至10个羟基的多元醇和平均分子量为约200至约600的聚乙二醇。另外，用于测定 该类型的γ-谷氨酰转移酶活性包括（a）包含缓冲液和γ-谷氨酰残基受体的缓冲介质; 和（b）底物溶液; 缓冲介质和底物溶液适于使得其预选等分试样在γ-谷氨酰转移酶存在下反应以产生吸收性裂解产物。 该试剂盒的特征在于底物溶液如上所述。 另外，用于确定该类型的γ-谷氨酰转移酶活性的方法包括（a）以任何预先选择的顺序接触含有缓冲液和γ-谷氨酰基残基受体的缓冲介质的等分试样，底物溶液和等分试样 的待测样品，从而形成裂解产物; 和（b）测量所述切割产物的吸光度。 该方法的特征在于底物溶液如上所述。

公开(公告)号：WO1983002341A1

公开(公告)日：1983-07-07

申请号：PCT/US1982001770

申请日：1982-12-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PARKES, Christopher, W. ,  FRANKLIN, Robert, C.

IPC: G05D13/62

CPC classification number: H02P23/186

Abstract: An improved motor speed control system for high speed, high resolution motor control applications such as centrifuges. A proportional or proportional plus integral motor control system is provided with improved circuitry which uses digital phase lock techniques to generate a speed difference signal that accurately reflects the difference between the actual and desired speed of the motor. This speed difference signal is used to adjust the speed of the motor until the actual and desired speeds become approximately equal to one another. An error integrating network may then be enabled to eliminate any remaining difference between actual and desired speed, thereby assuring a negligible speed error.

Abstract translation: 改进的电机速度控制系统，用于高速，高分辨率电机控制应用，如离心机。 提供了一种比例或比例加上积分的电机控制系统，其具有改进的电路，其使用数字锁相技术来产生精确反映电动机的实际速度和期望速度之间的差的速度差信号。 该速度差信号用于调整电机的速度，直到实际和所需速度彼此近似相等。 然后可以启用误差积分网络以消除实际速度和期望速度之间的任何剩余差异，从而确保可忽略的速度误差。

公开(公告)号：WO1983002323A1

公开(公告)日：1983-07-07

申请号：PCT/US1982001810

申请日：1982-12-21

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  Van VLIET, James, G. ,  BROWN, James, R.

IPC: G01J01/44

CPC classification number: G01J1/44 ,  H01J43/30

Abstract: Procédé et système de protection d'un détecteur photomultiplicateur utilisant une boucle de réaction négative pour maintenir la sortie du détecteur photomultiplicateur inférieure à une sortie prédéterminée. La boucle de réaction comprend un comparateur (38) sensible à la sortie du détecteur photomultiplicateur (14) et également sensible à un signal limite prédéterminé. La sortie du comparateur est appliquée à un amplificateur de sommation (60) qui reçoit également un signal d'ajustement de tension. La sortie de 'amplificateur de sommation (60) commande la sortie d'une alimentation de courant (12) qui est à son tour appliquée au détecteur photomultiplicateur (14).

公开(公告)号：WO1983002272A1

公开(公告)日：1983-07-07

申请号：PCT/US1982001787

申请日：1982-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BOWERS, Cyril, Y. ,  MOMANY, Frank, A.

IPC: C07C103/52

CPC classification number: C07K14/60 ,  A61K38/00 ,  Y02P20/55

Abstract: Novel peptides having the amino acid sequence selected from a group consisting of . In addition, this invention encompasses a combination of compounds comprising (a) at least one peptide having the formula III and (b) at least one growth promoting agent. These peptides and combinations act directly on the pituitary to release growth hormone therefrom.

Abstract translation: 具有选自的氨基酸序列的新型肽。 此外，本发明包括化合物的组合，其包含（a）至少一种具有式III的肽和（b）至少一种生长促进剂。 这些肽和组合直接作用于垂体以释放生长激素。