公开(公告)号：WO1984000978A1

公开(公告)日：1984-03-15

申请号：PCT/US1983001299

申请日：1983-08-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BECK, James, P. ,  LUNA, Carlos, E.

IPC: C12Q01/48

CPC classification number: C12Q1/48

Abstract: A substrate solution comprising (a) a substrate selected from a group consisting of salts of L- gamma -glutamyl-p-nitroanilide and L- gamma -glutamyl-3-carboxy-p-nitroanilide; and (b) a polyol selected from a group consisting of alkyl polyols containing 2 to 10 carbon atoms and 2 to 10 hydroxy groups and polyethylene glycol having an average molecular weight of from about 200 to about 600. Also, a kit for the determination of gamma -glutamyltransferase activity of the type comprising (a) a buffered medium comprising a buffer and a gamma -glutamyl residue acceptor; and (b) a substrate solution; the buffered medium and substrate solution being adapted such that preselected aliquots thereof can react in the presence of gamma -glutamyltransferase to produce an absorbing cleavage product. The kit is characterized in that the substrate solution is as described above. In, addition, a method for determining gamma -glutamyltransferase activity of the type comprising (a) contacting in any preselected order an aliquot of a buffered medium comprising a buffer and a gamma -glumtamyl residue acceptor, an aliquotof a substrate solution, and an aliquot of a sample to be assayed to thereby form a cleavage product; and (b) measuring the absorbance of said cleavage product. The method is characterized in that the substrate solution is as described above.

Abstract translation: 一种底物溶液，其包含（a）选自由L-γ-谷氨酰 - 对硝基苯胺和L-γ-谷氨酰基-3-羧基 - 对硝基苯胺的盐组成的组的底物; 和（b）选自含有2至10个碳原子的烷基多元醇和2至10个羟基的多元醇和平均分子量为约200至约600的聚乙二醇。另外，用于测定 该类型的γ-谷氨酰转移酶活性包括（a）包含缓冲液和γ-谷氨酰残基受体的缓冲介质; 和（b）底物溶液; 缓冲介质和底物溶液适于使得其预选等分试样在γ-谷氨酰转移酶存在下反应以产生吸收性裂解产物。 该试剂盒的特征在于底物溶液如上所述。 另外，用于确定该类型的γ-谷氨酰转移酶活性的方法包括（a）以任何预先选择的顺序接触含有缓冲液和γ-谷氨酰基残基受体的缓冲介质的等分试样，底物溶液和等分试样 的待测样品，从而形成裂解产物; 和（b）测量所述切割产物的吸光度。 该方法的特征在于底物溶液如上所述。

公开(公告)号：EP0118534B1

公开(公告)日：1987-04-08

申请号：EP83902968.3

申请日：1983-08-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECK, James, P. ,  LUNA, Carlos, E.

IPC: C12Q1/48

CPC classification number: C12Q1/48

Abstract: A substrate solution comprising (a) a substrate selected from a group consisting of salts of L-gamma-glutamyl-p-nitroanilide and L-gamma-glutamyl-3-carboxy-p-nitroanilide; and (b) a polyol selected from a group consisting of alkyl polyols containing 2 to 10 carbon atoms and 2 to 10 hydroxy groups and polyethylene glycol having an average molecular weight of from about 200 to about 600. Also, a kit for the determination of gamma-glutamyltransferase activity of the type comprising (a) a buffered medium comprising a buffer and a gamma-glutamyl residue acceptor; and (b) a substrate solution; the buffered medium and substrate solution being adapted such that preselected aliquots thereof can react in the presence of gamma-glutamyltransferase to produce an absorbing cleavage product. The kit is characterized in that the substrate solution is as described above. In, addition, a method for determining gamma-glutamyltransferase activity of the type comprising (a) contacting in any preselected order an aliquot of a buffered medium comprising a buffer and a gamma-glumtamyl residue acceptor, an aliquotof a substrate solution, and an aliquot of a sample to be assayed to thereby form a cleavage product; and (b) measuring the absorbance of said cleavage product. The method is characterized in that the substrate solution is as described above.

公开(公告)号：EP0118534A1

公开(公告)日：1984-09-19

申请号：EP83902968.0

申请日：1983-08-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECK, James, P. ,  LUNA, Carlos, E.

IPC: G01N ,  G01N33 ,  C12Q1

CPC classification number: C12Q1/48

Abstract: Solution de substrat comprenant (a) un substrat choisi dans un groupe formé des sels de L-gamma-glutamyl-p-nitroanilide et de L-gamma-glutamyl-3-carboxy-p-nitroanilide; et (b) un polyol choisi dans un groupe formé des polyols d'alcoyle contenant de 2 à 10 atomes de carbone et de 2 à 10 groupes hydroxy et de glycol de polyéthylène ayant un poids moléculaire moyen entre 200 environ et 600 environ. De plus, ensemble pour déterminer l'activité de la gamma-glutamyltransférase du type comprenant (a) un milieu tamponné formé d'un tampon et d'un accepteur de résidu de gamma-glutamyl; et (b) une solution de substrat; le milieu tamponné et la solution de substrat étant adaptés de façon que leurs aliquots présélectionnés puissent réagir en présence de gamma-glutamyltransférase afin de donner un produit de clivage absorbant. La caractéristique de cet ensemble est que la solution de substrat est semblable à la description ci-dessus. En outre, procédé de détermination de l'activité de la gamma-glutamyltransférase du type comprenant (a) la mise en contact dans n'importe quel ordre présélectionné d'un aliquot d'un milieu tamponné formé d'un tampon et d'un accepteur de résidu de gamma-glutamyl, d'un aliquot d'une solution de substrat et d'un aliquot d'un échantillon à titrer pour former de la sorte un produit de clivage; et (b) la mesure de l'absorbance dudit produit de clivage. La caractéristique de ce procédé est que la solution de substrat est semblable à la description ci-dessus.