公开(公告)号：WO1997038433A1

公开(公告)日：1997-10-16

申请号：PCT/US1997003867

申请日：1997-03-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  BALDESCHWIELER, John, D. ,  BEAUCHAMP, Jesse, L. ,  WIDMER, Marcel ,  O'CONNOR, Stephen, D. ,  KOSSAKOVSKI, Dmitri

IPC: H01H490/04

CPC classification number: G01Q30/02 ,  G01N1/4022 ,  G01N1/44 ,  G01N2001/028 ,  G01N2001/045 ,  G01Q60/22 ,  H01J49/0004 ,  H01J49/0463 ,  Y10S977/852 ,  Y10S977/862 ,  Y10T436/24 ,  Y10T436/25875

Abstract: The present invention discloses an improved method and apparatus for analyzing the surface of materials using sub-micron laser desorption gas phase analysis. The method uses a combination of near-field optical microscopy and time-of-flight mass spectroscopy.

Abstract translation: 本发明公开了一种使用亚微米激光解吸气相分析分析材料表面的改进方法和装置。 该方法采用近场光学显微镜和飞行时间质谱联用。

公开(公告)号：WO1997034483A1

公开(公告)日：1997-09-25

申请号：PCT/US1997004217

申请日：1997-03-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  MISLICK, Kimberly, Ann

IPC: A01N43/04

CPC classification number: C12N15/87 ,  A61K48/00 ,  C12N15/88

Abstract: Methods for controlling transfection efficiency mediated by complexes of cationic species and genetic material by adjusting the amount of membrane-associated proteoglycans and optionally adjusting the plasma concentration of glycosaminoglycans. Transfection efficiency is controlled by the amount of membrane-associated proteoglycans in the cell to be transfected and also by the plasma concentration of glycosaminoglycans. By increasing the amount of membrane-associated proteoglycans in the cell, and optionally decreasing the plasma concentration of glycosaminoglycans, the transfection efficiency can be increased. By decreasing the amount of membrane-associated proteoglycans in the cell, and optionally decreasing the plasma concentration of glycosaminoglycans, the transfection efficiency can be decreased. Transfection efficiency can be controlled, whether performed in vivo, ex vivo, or in vitro.

Abstract translation: 通过调节膜相关蛋白多糖的量和任选地调节葡糖胺聚糖的血浆浓度来控制由阳离子物质和遗传物质的复合物介导的转染效率的方法。 转染效率受转染细胞中膜相关蛋白聚糖的量以及糖胺聚糖血浆浓度的控制。 通过增加细胞中膜相关的蛋白聚糖的量，并且任选地降低葡糖胺聚糖的血浆浓度，可以提高转染效率。 通过减少细胞中膜相关的蛋白聚糖的量，并且任选地降低葡糖胺聚糖的血浆浓度，可以降低转染效率。 可以控制转染效率，无论是在体内，离体还是体外进行。

公开(公告)号：WO1997028641A1

公开(公告)日：1997-08-07

申请号：PCT/US1997001012

申请日：1997-01-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  FOSSUM, Eric, R. ,  KEMENY, Sabrina, E. ,  PAIN, Bedabrata

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: H04N03/14

CPC classification number: H01L27/14609 ,  G11C19/282 ,  H01L27/14643 ,  H04N5/3535 ,  H04N5/3575 ,  H04N5/365 ,  H04N5/374 ,  H04N9/045

Abstract: An imaging device formed as a monolithic complementary metal oxide semiconductor integrated circuit, the integrated circuit including a focal plane array of pixel cells (10), each one of the cells including a photogate (12) overlying the substrate (20) for accumulating photo-generated charge in an underlying portion of the substrate and a CCD device section (14) formed on the substrate adjacent the photogate (12) having a sensing node and at least one charge coupled device stage (16) for transferring charge from the underlying portion of the substrate to the sensing node. There is also a readout circuit, part of which can be disposed at the bottom of each column of cells and be common to all the cells in the column. The imaging device can also include an electronic shutter formed on the substrate adjacent the photogate (12), and/or a storage section to allow for simultaneous integration. In addition, the imaging device can include a multiresolution imaging circuit (604) to provide images of varying resolution. The multiresolution circuit could be employed in an array where the photosensitive portion of each pixel cell is a photodiode.

Abstract translation: 一种形成为单片互补金属氧化物半导体集成电路的成像装置，所述集成电路包括像素单元（10）的焦平面阵列，所述单元中的每一个包括覆盖所述基板（20）的光栅（12） 在衬底的下面部分中产生的电荷和形成在邻近具有感测节点的光栅（12）的衬底上的CCD器件部分（14）和至少一个电荷耦合器件级（16），用于将电荷从 基板到感应节点。 还有一个读出电路，其中的一部分可以设置在每一列单元的底部，并且与列中的所有单元通用。 成像装置还可以包括形成在邻近光栅（12）的基板上的电子快门和/或存储部分，以允许同时集成。 此外，成像装置可以包括多分辨率成像电路（604）以提供不同分辨率的图像。 多分辨率电路可以用于每个像素单元的光敏部分是光电二极管的阵列中。

公开(公告)号：WO1997028558A2

公开(公告)日：1997-08-07

申请号：PCT/US1997000927

申请日：1997-01-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  FOSSUM, Eric, R.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: H01L00/00

CPC classification number: H01L27/14609 ,  G11C19/282 ,  H01L27/14643 ,  H04N5/3535 ,  H04N5/3575 ,  H04N5/365 ,  H04N5/374 ,  H04N9/045

Abstract: An active pixel cell includes electronic shuttering capability. The cell can be "shuttered" to prevent additional charge accumulation. One mode transfers the current charge to a storage node that is blocked against accumulation of optical radiation. The charge is sampled from a floating node. Since the charge is stored, the node can be sampled at the beginning and the end of every cycle. Another aspect allows charge to spill out of the well whenever the charge amount gets higher than some amount, thereby providing anti blooming.

Abstract translation: 有源像素单元包括电子快门功能。 电池可以“快门”以防止额外的电荷积聚。 一种模式将当前电荷传输到阻止光辐射累积的存储节点。 从浮动节点采样电荷。 由于存储了电荷，所以可以在每个周期的开始和结束时采样节点。 另一方面，每当充电量高于一定量时，允许充电从井中溢出，从而提供防霜。

公开(公告)号：WO1997027304A1

公开(公告)日：1997-07-31

申请号：PCT/US1997001175

申请日：1997-01-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  ARNOLD, Frances, H. ,  MOORE, Jeffrey, C.

IPC: C12N15/55

CPC classification number: C12N9/18

Abstract: A method for isolating and identifying modified para-nitrobenzyl esterases which exhibit improved stability and/or esterase hydrolysis activity toward selected substrates and under selected reaction conditions relative to the unmodified para-nitrobenzyl esterase. The method involves preparing a library of modified para-nitrobenzyl esterase nucleic acid segments (genes) which have nucleotide sequences that differ from the nucleic acid segment which encodes for unmodified para-nitrobenzyl esterase. The library of modified para-nitrobenzyl nucleic acid segments is expressed to provide a plurality of modified enzymes. The clones expressing modified enzymes are then screened to identify which enyzmes have improved esterase activity by measuring the ability of the enzymes to hydrolyze the selected substrate under the selected reaction conditions. Specific modified para-nitrobenzyl esterases are disclosed which have improved stability and/or ester hydrolysis activity in aqueous or aqueous-organic media relative to the stability and/or ester hydrolysis activity of unmodified naturally occurring para-nitrobenzyl esterase.

Abstract translation: 分离和鉴定修饰的对硝基苄基酯酶的方法，其相对于未修饰的对硝基苄基酯酶，在选定的底物和选定的反应条件下表现出改进的稳定性和/或酯酶水解活性。 该方法包括制备具有不同于编码未修饰的对硝基苄基酯酶的核酸片段的核苷酸序列的经修饰的对硝基苄基酯酶核酸片段（基因）的文库。 表达修饰的对硝基苄基核酸片段的文库以提供多种修饰的酶。 然后筛选表达修饰的酶的克隆，以通过测量酶在所选择的反应条件下水解所选择的底物的能力来鉴定哪些烯胺具有改善的酯酶活性。 公开了相对于未修饰的天然存在的对硝基苄基酯酶的稳定性和/或酯水解活性，在水性或水性有机介质中具有改善的稳定性和/或酯水解活性的特异性修饰的对硝基苄基酯酶。

公开(公告)号：WO1997026870A1

公开(公告)日：1997-07-31

申请号：PCT/US1997001077

申请日：1997-01-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  STOWELL, Michael, H., B. ,  WANG, Guangyang ,  CHAN, Sunney, I.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: A61K09/70

CPC classification number: A61K9/1273

Abstract: Inorganic-organic hybrid mixture sol-gel encapsulated lipid vesicles which are composed of silyl lipids or a mixture of silyl lipids and phospholipids are provided. The present invention also provides encapsulated Langmuir Blogget (LB) membranes and biological macromolecules. The sol-gel encapsulated lipid vesicles, LB membranes and proteins possess a higher stability than conventional vesicles. Inorganic-organic hybrid mixture sol-gels are provided as novel sol-gel materials possessing desirable mechanical and physicochemical properties. Also provided are methods of preparing encapsulated lipid vesicles, LB membranes and proteins. Methods of performing renal dialysis using compositions of the invention are also provided.

Abstract translation: 提供由甲硅烷基脂质或甲硅烷基脂质和磷脂的混合物组成的无机 - 有机杂化混合物溶胶 - 凝胶包封的脂质囊泡。 本发明还提供封装的Langmuir Blogget（LB）膜和生物大分子。 溶胶 - 凝胶包封的脂质囊泡，LB膜和蛋白质具有比常规泡囊更高的稳定性。 提供无机 - 有机杂化混合物溶胶，作为具有理想的机械和物理化学性质的新型溶胶 - 凝胶材料。 还提供了制备包封的脂质囊泡，LB膜和蛋白质的方法。 还提供了使用本发明组合物进行肾透析的方法。

公开(公告)号：WO1997005712A1

公开(公告)日：1997-02-13

申请号：PCT/US1996012844

申请日：1996-08-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  YAO, Xiaotian, Steve ,  MALEKI, Lutfollah

IPC: H04B10/04

CPC classification number: G02F1/0123 ,  G02F2203/56

Abstract: A versatile photonic radio frequency (RF) oscillator (10) employs a single optical feedback loop, or multiple feedback loops of different delay times and including at least one optical feedback loop, to generate RF signals with ultra-low phase noise, narrow spectral linewidth, and a continuous wide tuning range of high resolution. Specifically, an electro-optical modulator (12) and a long optical fiber loop are implemented in one dual loop system. In addition, a light beam from a light source (40) can be directly modulated by using a single optical feedback signal, or a plurality of feedback signals from multiple feedback loops, to alter the electrical control signal to the light source. Furthermore, the disclosed system supports both electrical and optical RF outputs (32). External optical injection (28) and electrical injection (30) can be implemented.

Abstract translation: 多功能光子射频（RF）振荡器（10）采用单个光反馈回路或不同延迟时间的多个反馈回路，并且包括至少一个光反馈回路，以产生具有超低相位噪声，窄谱线宽度的RF信号 ，以及高分辨率的连续宽调谐范围。 具体地，在一个双环系统中实现电光调制器（12）和长光纤回路。 此外，来自光源（40）的光束可以通过使用单个光学反馈信号或来自多个反馈回路的多个反馈信号来直接调制，以改变到光源的电气控制信号。 此外，所公开的系统支持电和RF射频输出（32）。 可以实现外部光学注入（28）和电子注入（30）。

公开(公告)号：WO1996040877A1

公开(公告)日：1996-12-19

申请号：PCT/US1996009781

申请日：1996-06-06

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  ANDERSON, David, J.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: C12N05/10

CPC classification number: C12N15/86 ,  A61K38/162 ,  A61K48/00 ,  C12N15/907 ,  C12N2740/10043 ,  C12N2740/13043 ,  C12N2800/30 ,  C12N2840/20 ,  C12N2840/203

Abstract: Methods and compositions for the conditional immortalization of cells are provided.

Abstract translation: 提供了细胞条件永生化的方法和组合物。

公开(公告)号：WO1996031521A1

公开(公告)日：1996-10-10

申请号：PCT/US1996004546

申请日：1996-04-03

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  VARSHAVSKY, Alexander ,  JOHNSTON, Jennifer

IPC: C07H19/00

CPC classification number: C12N9/003 ,  C07K14/00 ,  C07K14/001 ,  C07K14/395 ,  C07K2319/00 ,  C07K2319/42 ,  C07K2319/61 ,  C07K2319/95 ,  C12N9/0028 ,  C12N9/12 ,  C12N15/11 ,  C12N15/62

Abstract: Disclosed herein is a heat-inducible N-degron module. A heat-inducible N-degron module is a protein or peptide bearing a destabilizing N-terminal amino acid residue which becomes a substrate of the N-end rule pathway only at a temperature high enough to result in at least partial unfolding of the protein. At this elevated (nonpermissive) temperature, the heat-inducible N-degron module (and any protein or peptide attached at its C-terminus) is rapidly degraded in a cell in which the N-end rule pathway is operative. Also disclosed are DNA and protein fusion constructs, methods for screening for additional heat-inducible N-degron modules and methods for using the disclosed heat-inducible N-degron modules. Also disclosed are methods for inhibiting the degradation of a protein bearing an N-degron in which a low molecular mass, cell penetrating ligand is prebound to the protein.

Abstract translation: 本文公开了一种热诱导型N-离子解聚模块。 热诱导型N-解体模块是具有不稳定的N-末端氨基酸残基的蛋白质或肽，其仅在足以导致蛋白质的至少部分展开的温度下成为N端规则通路的底物。 在这种升高的（非允许的）温度下，在N端规则通路可操作的细胞中，热诱导型N-脱落元件模块（以及在其C-末端附着的任何蛋白质或肽）被快速降解。 还公开了DNA和蛋白质融合构建体，用于筛选额外的热诱导性N-脱落模块的方法以及使用所公开的热诱导型N-脱落伞模块的方法。 还公开了抑制具有低分子量的细胞穿透性配体与蛋白质结合的N-脱落蛋白的蛋白质的降解的方法。

公开(公告)号：WO1996011712A2

公开(公告)日：1996-04-25

申请号：PCT/US1995014621

申请日：1995-10-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  KAYYEM, Jon, F. ,  MEADE, Thomas, J. ,  FRASER, Scott, E.

Inventor： CALIFORNIA INSTITUTE OF TECHNOLOGY

IPC: A61K47/48

CPC classification number: A61K49/146 ,  A61K47/645 ,  A61K49/085 ,  Y10S977/808 ,  Y10S977/927 ,  Y10S977/93

Abstract: A delivery vehicle is described that is capable of being specifically bound to and taken into targeted cells, delivering numerous physiological agents, particularly paramagnetic ions for magnetic resonance imaging (MRI) of the cells. The delivery vehicle comprises a polymeric molecule having a net positive charge complexed with another polymeric molecule having a net negative charge. Cell targeting moieties and physiological agents, including contrast agents and therapeutic agents, are attached to one or both of the polymeric molecules. In one embodiment, the polymeric molecule having a net negative charge is a nucleic acid. Thus, the delivery vehicles can be used in clinical protocols in which nucleic acids for gene therapy and agents for MRI contrast are co-transported to specific cells allowing medical imaging monitoring of nucleic acid delivery.

Abstract translation: 描述了能够特异性结合并进入靶细胞的递送载体，递送许多生理剂，特别是顺磁离子用于细胞的磁共振成像（MRI）。 递送载体包含具有与具有净负电荷的另一个聚合物分子络合的净正电荷的聚合物分子。 细胞靶向部分和生理剂，包括造影剂和治疗剂，连接到一个或两个聚合物分子。 在一个实施方案中，具有净负电荷的聚合物分子是核酸。 因此，递送载体可以用于其中用于基因治疗的核酸和用于MRI对比的药剂共同转运到特定细胞的临床方案，允许核酸递送的医学成像监测。