公开(公告)号：US20160011114A1

公开(公告)日：2016-01-14

申请号：US14769885

申请日：2014-02-20

Applicant: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIO ,  USHIO DENKI KABUSHIKI KAISHA

Inventor： Yuji OKI ,  Kinichi MORITA

IPC: G01N21/64 ,  G01N33/53 ,  G01N33/536 ,  B01L3/00 ,  G01N33/569

CPC classification number: G01N21/6428 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0403 ,  B01L2400/0442 ,  B01L2400/046 ,  G01N21/03 ,  G01N21/05 ,  G01N33/5304 ,  G01N33/536 ,  G01N33/56966 ,  G01N2021/6482 ,  G01N2201/0245

Abstract: In order to carry out various analyses at high accuracy in a site where analysis is needed using a disposable microchip, the microchip is disposed on a display of a tablet terminal device, and a sample or specimen is dropped into a flow path of the microchip to trigger a reaction of the sample in the microchip. Light emitted from the display of the tablet terminal device is applied to the microchip, and the reaction caused by the applied light in the microchip is measured. When an induced fluorescence method using the applied light is adopted as a measuring method, fluorescence that corresponds to the reaction is observed, and detected by, for example, a built-in camera of the tablet terminal device. The detection signal is analyzed by an arithmetic unit of the tablet terminal device.

Abstract translation: 为了在使用一次性微芯片的需要分析的场所以高精度进行各种分析，将微芯片设置在平板电脑终端装置的显示器上，将样品或样品落入微芯片的流路中， 触发微芯片中样品的反应。 从平板电脑终端装置的显示器发射的光被施加到微芯片，并且测量由微芯片中的施加的光引起的反应。 当采用使用所施加的光的诱导荧光方法作为测量方法时，观察到与反应相对应的荧光，并且通过例如平板电脑终端装置的内置照相机进行检测。 检测信号由平板电脑终端装置的算术单元分析。

公开(公告)号：US20140349381A1

公开(公告)日：2014-11-27

申请号：US14219905

申请日：2014-03-19

Applicant: Micronics, Inc.

Inventor： C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

IPC: G01N21/64

CPC classification number: G01N21/6456 ,  B01L7/52 ,  C12M23/16 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0245

Abstract: An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general.

Abstract translation: 公开了用于液体样品中的荧光测定的仪器。 仪器可以包括用于监测与靶分析物相关联的第一荧光团的多个光学通道和与对照相关联的第二荧光团。 所公开的仪器可用于任何数量的应用，包括基于目标核酸的PCR扩增和荧光测定的微流体分子生物学测定。

公开(公告)号：US08741234B2

公开(公告)日：2014-06-03

申请号：US13337900

申请日：2011-12-27

Applicant: Tzu-Yu Wang ,  Ron Bardell ,  Lynn Seifried

Inventor： Tzu-Yu Wang ,  Ron Bardell ,  Lynn Seifried

IPC: G01N21/00

CPC classification number: B01L3/502738 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N21/05 ,  G01N21/51 ,  G01N21/532 ,  G01N33/4915 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/513 ,  G01N2035/00158 ,  G01N2201/0245

Abstract: A disposable blood analysis cartridge for analyzing a blood sample including an optical absorbance measurement channel is described. The optical absorbance measurement channel includes a plasma separation region and at least one sub channel including a cuvette that is in fluid communication with the plasma separation region and configured to receive a plasma portion of a blood sample that has been passed through the plasma separation region. A negative pressure may be applied to the cartridge to draw the sample through the plasma separation region and into the sub channel including the cuvette.

Abstract translation: 描述了用于分析包括光吸收测量通道的血液样品的一次性血液分析盒。 光吸收测量通道包括等离子体分离区域和至少一个子通道，其包括与等离子体分离区域流体连通并配置成接收已经通过血浆分离区域的血液样品的血浆部分的比色皿。 可以向盒施加负压以将样品通过等离子体分离区域并进入包括比色皿的子通道。

公开(公告)号：US20140045187A1

公开(公告)日：2014-02-13

申请号：US14054729

申请日：2013-10-15

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

Abstract translation: 提供了一种光学仪器，用于同时用由光源产生的激发光束照射两个或更多个间隔开的反应区域。 光源可以包括发光二极管的区域光阵列，一个或多个固态激光器，一个或多个微线激光器，或其组合。 根据各种实施例，菲涅耳透镜可以沿着光源和反应区域之间的光束槽设置。 还提供了使用光学仪器的分析方法。

公开(公告)号：US08557566B2

公开(公告)日：2013-10-15

申请号：US11333483

申请日：2006-01-17

Applicant: Michael R. Gambini ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Susan Atwood Stone ,  Judith K. Atwood

Inventor： Michael R. Gambini ,  John G. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

IPC: C12M1/34 ,  C12M3/00

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US20130164854A1

公开(公告)日：2013-06-27

申请号：US13337900

申请日：2011-12-27

Applicant: Tzu-Yu Wang ,  Ron Bardell ,  Lynn Seifried

Inventor： Tzu-Yu Wang ,  Ron Bardell ,  Lynn Seifried

IPC: G01N33/48 ,  B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N21/05 ,  G01N21/51 ,  G01N21/532 ,  G01N33/4915 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/513 ,  G01N2035/00158 ,  G01N2201/0245

Abstract: A disposable blood analysis cartridge for analyzing a blood sample including an optical absorbance measurement channel is described. The optical absorbance measurement channel includes a plasma separation region and at least one sub channel including a cuvette that is in fluid communication with the plasma separation region and configured to receive a plasma portion of a blood sample that has been passed through the plasma separation region. A negative pressure may be applied to the cartridge to draw the sample through the plasma separation region and into the sub channel including the cuvette.

Abstract translation: 描述了用于分析包括光吸收测量通道的血液样品的一次性血液分析盒。 光吸收测量通道包括等离子体分离区域和至少一个子通道，其包括与等离子体分离区域流体连通并配置成接收已经通过血浆分离区域的血液样品的血浆部分的比色皿。 可以向盒施加负压以将样品通过等离子体分离区域并进入包括比色皿的子通道。

公开(公告)号：US20130111968A1

公开(公告)日：2013-05-09

申请号：US13667033

申请日：2012-11-02

Applicant: SERAMUN DIAGNOSTICA GMBH

Inventor： Tomas Porstmann ,  Klemens Loster ,  Erik Hochel ,  Christoph Lorenz ,  Peter Pas

IPC: G01N33/00 ,  G01N21/00

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  B01L2200/026 ,  B01L2200/143 ,  B01L2200/18 ,  B01L2300/0829 ,  G01N21/13 ,  G01N21/253 ,  G01N35/028 ,  G01N2035/042 ,  G01N2201/0245

Abstract: A sample analysis device is provided for determining samples in a sample matrix with several sample volumes. The device includes: a receiving device with a receiving facility for a sample matrix comprising a plurality of sample volumes; a first measuring device configured to determine samples in the sample matrix, by means of wet measuring; a second measuring device, configured to determine further samples in the sample matrix, which further samples differ from the samples, or in another sample matrix, by means of dry measuring; and a transfer device, which is coupled to the receiving device and is configured to transfer the receiving device between a loading position and a plurality of measuring positions in such a manner that the receiving facility can be transferred to various measuring positions that are either associated with the first or with the second measuring device.

Abstract translation: 提供样品分析装置用于确定具有多个样品体积的样品基质中的样品。 该装置包括：具有用于包括多个样本体积的样本矩阵的接收设备的接收设备; 第一测量装置，被配置为通过湿测量来确定样品基质中的样品; 第二测量装置，其被配置为通过干式测量来确定所述样品基质中的进一步样品与样品不同的另外的样品，或另一样品样品中的样品; 以及传送装置，其耦合到所述接收装置并且被配置为在所述接收装置在装载位置和多个测量位置之间传送所述接收装置，使得所述接收装置可以被传送到与 第一或第二测量装置。

公开(公告)号：US20130011912A1

公开(公告)日：2013-01-10

申请号：US13536592

申请日：2012-06-28

Applicant: C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

Inventor： C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

IPC: C12M1/34 ,  G01N21/64

CPC classification number: G01N21/6456 ,  B01L7/52 ,  C12M23/16 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0245

Abstract: An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general.

Abstract translation: 公开了用于液体样品中荧光测定的仪器。 仪器可以包括用于监测与靶分析物相关联的第一荧光团的多个光学通道和与对照相关联的第二荧光团。 所公开的仪器可用于任何数量的应用，包括基于目标核酸的PCR扩增和荧光测定的微流体分子生物学测定。

公开(公告)号：US08329453B2

公开(公告)日：2012-12-11

申请号：US13191120

申请日：2011-07-26

Applicant: C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

Inventor： C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

IPC: C12M1/34

CPC classification number: G01N21/6456 ,  B01L7/52 ,  C12M23/16 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0245

Abstract: Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection. Sensitivity and resistance to bubble interference during scanning are shown to be improved by use of a heating block with reflective mirror face in intimate contact with a thermo-optical window enclosing the liquid sample.

Abstract translation: 公开了一种用于液体样品中的荧光测定的紧凑型微处理器控制仪器，该仪器具有带有用于接收微流控盒的对接槽的浮动台和具有用于控制源LED的板上嵌入式微处理器的扫描检测器头，发射信号放大和 在检测器头内的隔离，低噪声，高增益环境中进行滤波。 多个光通道可并入扫描头。 在优选的配置中，使用双通道光学器件验证测定，用于监测与靶分析物相关联的第一荧光团和与对照相关联的第二荧光团。 应用包括基于目标核酸的PCR扩增和荧光测定的分子生物测定法，其中许多在检测期间需要温度控制。 通过使用具有与包围液体样品的热光学窗口紧密接触的反射镜面的加热块，可以改善扫描期间对气泡干扰的灵敏度和抗性。

公开(公告)号：US08300218B2

公开(公告)日：2012-10-30

申请号：US13355401

申请日：2012-01-20

Applicant: Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

Inventor： Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

IPC: G01N21/00

CPC classification number: G01N21/3504 ,  G01K13/02 ,  G01K2013/024 ,  G01N1/2247 ,  G01N21/05 ,  G01N2201/0245

Abstract: Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Abstract translation: 气体分析仪系统和测量气体浓度的方法，特别是气体组分的干摩尔分数。 这些系统和方法允许快速测量多种环境监测应用的气体密度和/或气体的干摩尔分数，包括高速通量测量。 新的耦合设计允许无需移除封闭流动通道的电池，从而可以对光学部件进行现场清洁。