公开(公告)号：EP0521128B1

公开(公告)日：1996-03-13

申请号：EP92903877.6

申请日：1991-12-18

Applicant: EASTMAN KODAK COMPANY

Inventor： MARCUS, Michael, Alan

IPC: G01N21/75 ,  G01N21/77 ,  G01N21/78

CPC classification number: G01N21/648 ,  G01N21/1717 ,  G01N21/7703 ,  G01N2021/1725 ,  G01N2021/772 ,  G01N2021/773 ,  G01N2021/7786 ,  G01N2201/122

Abstract: Apparatus and method are disclosed in which a beam of radiant energy (16) is passed into a medium at a wavelength selected to cause a response of an analyte in the medium or of a sensor (10, 14) exposed to the medium, the sensor comprising a reagent whose optical properties change in response to the presence of the analyte in the medium. A perturbing pulse of energy (22) is passed into the medium to alter the response of the analyte or reagent to the beam of radiation; and the time rates of change in the intensity of the transmitted radiant energy are measured (18, 24, 26, 28) while the response is changing due to the influence of the perturbing pulse, after the perturbing pulse starts or ends or both, such rates of change being proportional to the concentration of the analyte in the medium. Both the beam of radiant energy and the perturbing pulse may be passed through optic fibers into the medium or sensor.

公开(公告)号：EP0507930A1

公开(公告)日：1992-10-14

申请号：EP91920381.0

申请日：1991-10-30

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： MORRIS, Roger, James ,  BASCOMB, Shoshana ,  OLSON, Carolyn, Shelchuk

IPC: C12Q1 ,  G01N21

CPC classification number: G01N21/17 ,  G01N21/534 ,  G01N21/78 ,  G01N2201/122 ,  Y10S436/80

Abstract: Procédé de détermination de la concentration d'une substance quelconque dans une réaction colorimétrique, turbidimétrique ou néphélométrique à l'aide d'un détecteur fluorimétrique mesurant l'intensité de la fluorescence. Notamment, on peut surveiller un changement de couleur en examinant la mesure de l'intensité de la fluorescence d'un fluorogène dans une matrice inerte. Le spectre d'absorption du chromophore peut chevaucher le spectre d'excitation et/ou d'émission du fluorophore, ce qui permet à la modification de la fluorescence d'être associée à l'intensité de couleur de la réaction et donc également à la quantité de ladite substance.

公开(公告)号：EP0301699A2

公开(公告)日：1989-02-01

申请号：EP88305732.5

申请日：1988-06-23

Applicant: Utah State University Foundation

Inventor： Richardson, Gary H.

IPC: G01N21/51 ,  G01N21/25 ,  G01N33/04 ,  G01N33/15

CPC classification number: G01N21/4738 ,  C12M41/36 ,  G01N21/25 ,  G01N33/04 ,  G01N2201/0446 ,  G01N2201/122

Abstract: Methods and apparatus for determining the bioactivity of liquid biological mixtures. The method of the present invention involves obtaining a suitable quantity of liquid biological mixture. Once the sample is obtained, visual spectrum light is directed into the liquid biological mixture and reflectance of the light from the mixture is detected and quantified.
The apparatus of the present invention incorporates a microcolorimeter with high energy source as a reflectance detector. The microcolorimeter is able to measure energy in a smaller area, such as 3 to 8 millimeters in diameter, and provide estimates of bioactivity in less than 30 minutes to 32 hours, as opposed to the 24 hour to 9 days which is typical of traditional microbiological methods.
The method of the present invention could involve continuous measurements by recycling plates past the detector at regular intervals until the end point is detected. Thus, the detector could be fitted with an appropriate incubator environment which would have controlled robotic devices to receive, identify, and position plates for storage, reading and for discard. Data is collected and compared by computer. The computer calculates the desired information on bioactivity.
By providing a detector that produces "tristimulus" color values it is possible to use traditional dyes used in microbiological research and testing. Even abandoned dyes could be restored to useful measurement including litmus, methylene blue, and resazurin.

Abstract translation: 用于测定液体生物混合物的生物活性的方法和装置。 本发明的方法涉及获得适量的液体生物混合物。 一旦获得样品，将视觉光谱引导到液体生物混合物中，并检测和量化来自混合物的光的反射率。 本发明的装置包含具有高能量源的微型色度计作为反射检测器。 显微比色计能够在较小的区域测量能量，例如直径为3至8毫米，并且在少于30分钟至32小时内提供生物活性的估计，而不是24小时至9天，这是传统微生物学的典型 方法。 本发明的方法可以包括通过循环板经过检测器以规则的间隔连续测量直到检测到终点。 因此，检测器可以配备适当的孵化器环境，其将具有受控的机器人装置来接收，识别和定位板以用于存储，读取和丢弃。 收集数据并通过电脑进行比较。 计算机计算所需的生物活性信息。 通过提供产生“三刺激”颜色值的检测器，可以使用用于微生物研究和测试的传统染料。 甚至废弃的染料也可以恢复到有用的测量，包括石蕊，亚甲基蓝和天竺葵素。

公开(公告)号：EP0223427A1

公开(公告)日：1987-05-27

申请号：EP86308159.2

申请日：1986-10-21

Applicant: ALTA DIAGNOSTICS MACHINES LTD

Inventor： Bradwell, Arthur Randell, c/o The Medical School ,  Deverill, Ian

IPC: G01N33/53 ,  G01N33/557 ,  G01N21/47

CPC classification number: G01N33/53 ,  G01N21/47 ,  G01N33/557 ,  G01N2201/122 ,  Y10S436/805 ,  Y10S436/825 ,  Y10S436/909

Abstract: A method and apparatus for use in quantifying, in a whole blood sample in which the red cells are lysed, a component which will react with a reagent to form an antigen-antibody complex, the method comprising mixing the sample with the reagent to obtain the complex, exposing the sample to a source of radiation and measuring the intensity of radiation scattered through a given angle by the complex, and the apparatus including means (13) for receiving said sample which has been treated with a reagent which forms an antigen/antibody complex with a component of the sample, said means being transparent to radiation having a wavelength falling within a given band width, typically 460-530 nm a source of radiation (10, 11) having a radiation within said band width and means (14) for detecting the intensity of said radiation (17) which is scattered through a given angle by the sample.

公开(公告)号：EP3343207A1

公开(公告)日：2018-07-04

申请号：EP16838909.6

申请日：2016-06-28

Applicant: Hitachi High-Technologies Corporation

Inventor： INABE, Toshiyuki ,  MAKINO, Akihisa ,  ADACHI, Sakuichiro ,  YABUTANI, Chie

IPC: G01N21/82

CPC classification number: G01N33/542 ,  G01N21/253 ,  G01N21/272 ,  G01N21/51 ,  G01N21/82 ,  G01N33/52 ,  G01N33/86 ,  G01N35/00663 ,  G01N2035/00673 ,  G01N2201/121 ,  G01N2201/122 ,  G01N2201/1242 ,  G01N2201/1247 ,  G01N2201/127

Abstract: In order to enable increase in a measurement range for an interference substance regardless of before or after mixing of a reagent with a sample, and further to realize measurement of the level of the interference substance and measurement of the sample under the same measurement conditions, this automatic analysis device is provided with (1) a measurement mechanism having a light measuring unit having installed therein a reaction container in which the sample is dispensed, a light source which emits light to the reaction container, and a detection unit that detects scattered light from the sample in the reaction container, (2) an amplifier circuit unit having an adder-subtractor that adds or subtracts a correction signal to or from a first measurement signal from the detection unit, and an amplifier circuit which amplifies the output signal by the adder-subtractor at a fixed amplification rate to output a second measurement signal, and (3) an arithmetic operation unit which calculates the correction signal on the basis of a difference between the signal level of the second measurement signal and a target value, and which executes an analysis action on the basis of the second measurement signal after correction by means of the correction signal.

公开(公告)号：EP3052924A1

公开(公告)日：2016-08-10

申请号：EP14777135.6

申请日：2014-09-30

Applicant: Renishaw PLC

Inventor： MASON, Thomas Anthony

IPC: G01N21/65 ,  G01J3/44 ,  G01J3/28

CPC classification number: G01N21/65 ,  G01J3/0208 ,  G01J3/0237 ,  G01J3/0248 ,  G01J3/2803 ,  G01J3/2823 ,  G01J3/44 ,  G01J2003/2826 ,  G01N2201/025 ,  G01N2201/06113 ,  G01N2201/103 ,  G01N2201/11 ,  G01N2201/117 ,  G01N2201/122

Abstract: This invention concerns spectroscopy apparatus comprising a light source (101) arranged to generate a light profile (110) on a sample, a photodetector (103) having at least one photodetector element (104) for detecting characteristic light generated from interaction of the sample with light from the light source (101), a support (109) for supporting the sample, the support (109) movable relative to the light profile (110), and a processing unit (121). The processing unit (121) is arranged to associate a spectral value recorded by the photodetector element (104) at a particular time with a point on the sample predicted to have generated the characteristic light recorded by the photodetector element (104) at the particular time based on relative motion anticipated to have occurred between the support (109) and the light profile (110).

公开(公告)号：EP1631829B1

公开(公告)日：2016-04-13

申请号：EP04736157.1

申请日：2004-06-04

Applicant: GE Healthcare Bio-Sciences AB

Inventor： ANDERSSON, Karl ,  WASS, Christina

IPC: G01N35/08

CPC classification number: G01N21/05 ,  G01N21/553 ,  G01N35/08 ,  G01N2021/0162 ,  G01N2021/1723 ,  G01N2035/0097 ,  G01N2201/122

公开(公告)号：EP0250565B1

公开(公告)日：1992-04-01

申请号：EP87900587.4

申请日：1986-12-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： PANG, Wing, S. ,  OKAWA, Dobson, M. ,  KAN, Peter ,  SHIBATA, George, K.

IPC: G01N35/00

CPC classification number: G01N21/0332 ,  G01N21/51 ,  G01N33/53 ,  G01N35/00 ,  G01N2201/122 ,  Y10S435/808 ,  Y10S436/808 ,  Y10S436/909

Abstract: A system for analyzing a chemical reaction provides control of the temperature and volume of the reagents to improve the accuracy and precision in quantitive measurements of specific proteins and other immunochemistries in body fluids. The reaction occurs in a cuvette (48, 50) within a nephelometric optics module (44, 46). A sensor (246, 248) senses the temperatures of reaction buffer liquids as they flow into the cuvette (48, 50), and a heat exchanging device (250, 252) increases or decreases the temperatures of the buffer liquids. A control circuit (242) responsive to the temperature sensor (246, 248) controls the heat exchanging device (218) to maintain the temperature of the buffer liquids and the cuvette (48, 50) within a selected temperature range. The system may also include a sample pickup station (18), sample probe (22) means for withdrawing a selected sample from the sample pickup station (18), a sample preparation station, and sample transport (20) means for carrying said sample from the sample preparation station to the reaction cuvette (48, 50). The system may include an antibody pickup station (108), antibody probe (110) means for withdrawing an antibody from the antibody pickup station (108), an antibody preparation station, and antibody transport (106) means for carrying the antibody from the antibody preparation station to the reaction cuvette (48, 50).

公开(公告)号：EP0301699A3

公开(公告)日：1990-02-28

申请号：EP88305732.5

申请日：1988-06-23

Applicant: Utah State University Foundation

Inventor： Richardson, Gary H.

IPC: G01N21/51 ,  G01N21/25 ,  G01N33/04 ,  G01N33/15

CPC classification number: G01N21/4738 ,  C12M41/36 ,  G01N21/25 ,  G01N33/04 ,  G01N2201/0446 ,  G01N2201/122

Abstract: Methods and apparatus for determining the bioactivity of liquid biological mixtures. The method of the present invention involves obtaining a suitable quantity of liquid biological mixture. Once the sample is obtained, visual spectrum light is directed into the liquid biological mixture and reflectance of the light from the mixture is detected and quantified. The apparatus of the present invention incorporates a microcolorimeter with high energy source as a reflectance detector. The microcolorimeter is able to measure energy in a smaller area, such as 3 to 8 millimeters in diameter, and provide estimates of bioactivity in less than 30 minutes to 32 hours, as opposed to the 24 hour to 9 days which is typical of traditional microbiological methods. The method of the present invention could involve continuous measurements by recycling plates past the detector at regular intervals until the end point is detected. Thus, the detector could be fitted with an appropriate incubator environment which would have controlled robotic devices to receive, identify, and position plates for storage, reading and for discard. Data is collected and compared by computer. The computer calculates the desired information on bioactivity. By providing a detector that produces "tristimulus" color values it is possible to use traditional dyes used in microbiological research and testing. Even abandoned dyes could be restored to useful measurement including litmus, methylene blue, and resazurin.

公开(公告)号：EP0250565A1

公开(公告)日：1988-01-07

申请号：EP87900587.0

申请日：1986-12-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： PANG, Wing, S. ,  OKAWA, Dobson, M. ,  KAN, Peter ,  SHIBATA, George, K.

IPC: G01N33 ,  G01N21 ,  G01N35

CPC classification number: G01N21/0332 ,  G01N21/51 ,  G01N33/53 ,  G01N35/00 ,  G01N2201/122 ,  Y10S435/808 ,  Y10S436/808 ,  Y10S436/909

Abstract: Un système d'analyse d'une réaction chimique permet de commander la température et le volume des réactifs dans le but d'améliorer la précision des mesures quantitatives de protéines spécifiques et autres éléments immunochimiques dans des fluides du corps. La réaction a lieu dans une cuvette (48, 50) dans un module optique néphélométrique (44, 46). Un détecteur (246, 248) détecte les températures des liquides-tampon de la réaction au fur et à mesure qu'ils s'écoulent dans la cuvette (48, 50), et un dispositif d'échange de chaleur (250, 252) augmente ou diminue les températures des liquides-tampon. Un circuit de commande (242) sensible au détecteur de température (246, 248) commande le dispositif d'échange de chaleur (218) pour maintenir la température des liquides-tampon et de la cuvette (48, 50) dans une plage de température sélectionnée. Le système peut également inclure un poste de prélèvement d'échantillons (18), des moyens de sonde d'échantillon pour retirer un échantillon sélectionné du poste de prélèvement d'échantillon (18), un poste de préparation d'échantillons, et des moyens de transport d'échantillons (20) pour transporter l'échantillon depuis le poste de préparation d'échantillons jusqu'à la cuvette de réaction (48, 50). Le système peut inclure un poste de prélèvement d'anticorps (108), des moyens de sonde d'anticorps (11) pour retirer un anticorps du poste de prélèvement d'anticorps (108), un poste de préparation d'anticorps et des moyens de transport d'anticorps (106) pour transporter l'anticorps depuis le poste de préparation d'anticorps jusqu'à la cuvette de réaction (48, 50).