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公开(公告)号：WO2014070826A1
公开(公告)日：2014-05-08
申请号：PCT/US2013/067390
申请日：2013-10-30
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： FOEGEDING, Nora, J. , GRAHAM, Carrie, A. , ECKHARDT, Allen, E.
CPC classification number: C12Q1/32 , G01N33/6893 , G01N2333/90206 , G01N2800/04 , G01N2800/7085
Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very- long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme- specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.
Abstract translation: 提供了一种用于进行中链酰基辅酶A脱氢酶（MCAD）和非常长链酰基辅酶A脱氢酶（VCAD）酶活性测定的方法。 该方法可以包括但不限于制备样品; 制备酶特异性底物/试剂混合物; 将制备的样品的等分试样与酶特异性底物/试剂混合物的等分试样混合; 读取吸光度在约600nm范围内; 孵育制备的样品和酶特异性底物/试剂混合物; 并以各种时间间隔读取约600nm范围内的吸光度。
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公开(公告)号：WO2013016413A3
公开(公告)日：2013-01-31
申请号：PCT/US2012/048127
申请日：2012-07-25
Applicant: ADVANCED LIQUID LOGIC INC , BAUER, Craig , SISTA, Ramakrishna , BORT, Donovan
Inventor： BAUER, Craig , SISTA, Ramakrishna , BORT, Donovan
Abstract: Droplet actuator apparatus and system are provided. An apparatus comprises: a microfluidics system having an insertion slot for insertion of a droplet actuator; a bottom plate against which the droplet actuator would slide when inserting the droplet actuator into the insertion slot; and means for forcing a substrate in the droplet actuator parallel to the bottom plate, wherein warpage in the substrate is corrected.
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公开(公告)号：WO2013016413A2
公开(公告)日：2013-01-31
申请号：PCT/US2012048127
申请日：2012-07-25
Applicant: ADVANCED LIQUID LOGIC INC , BAUER CRAIG , SISTA RAMAKRISHNA , BORT DONOVAN
Inventor： BAUER CRAIG , SISTA RAMAKRISHNA , BORT DONOVAN
CPC classification number: B01L3/502715 , B01L3/502792 , B01L2200/025 , B01L2200/04 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , B23P11/02 , Y10T29/49945
Abstract: An apparatus, comprising a microfluidics system having an insertion slot for insertion of a droplet actuator; a bottom plate against which the droplet actuator would slide when inserting the droplet actuator into the insertion slot; and means for forcing a substrate in the droplet actuator parallel to the bottom plate, wherein warpage in the substrate is corrected.
Abstract translation: 一种装置，包括具有用于插入液滴致动器的插入口的微流体系统; 当将液滴致动器插入插槽时液滴致动器将滑动的底板; 以及用于强制平行于底板的液滴致动器中的基板的装置，其中修正了基板中的翘曲。
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公开(公告)号：WO2013006312A2
公开(公告)日：2013-01-10
申请号：PCT/US2012/044235
申请日：2012-06-26
Applicant: ADVANCED LIQUID LOGIC INC , FOLEY, Jennifer , BURDE, Stefan
Inventor： FOLEY, Jennifer , BURDE, Stefan
CPC classification number: C12Q1/703 , B01L3/502784 , B01L3/502792 , B01L7/525 , B01L2200/0678 , B01L2200/16 , B01L2300/0816 , B01L2400/0427 , C12Q1/6806 , C12Q2523/308 , C12Q2527/125 , C12Q2531/113 , C12Q2563/159 , C12Q2565/607
Abstract: A method of providing a droplet comprising one or more reagents, the method comprising, depositing a first aqueous droplet comprising the one or more reagents on a surface; drying the droplet to yield a dried composition on the surface comprising the one or more reagents; covering the dried composition with oil; and causing a second aqueous droplet in the oil to contact the dried composition and thereby resuspend one or more reagents.
Abstract translation: 提供包含一种或多种试剂的液滴的方法，所述方法包括：将包含所述一种或多种试剂的第一含水液滴沉积在表面上; 干燥所述液滴以在包含所述一种或多种试剂的表面上产生干燥的组合物; 用油覆盖干燥的组合物; 并使油中的第二含水液滴接触干燥的组合物，从而重悬一种或多种试剂。
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公开(公告)号：WO2012154794A2
公开(公告)日：2012-11-15
申请号：PCT/US2012/037036
申请日：2012-05-09
Applicant: ADVANCED LIQUID LOGIC, INC. , GRAHAM, Carrie , ECKHARDT, Alan , PERKINS, Lisa
Inventor： GRAHAM, Carrie , ECKHARDT, Alan , PERKINS, Lisa
CPC classification number: G01N1/405 , C12N9/16 , C12N9/2402 , C12N9/2408 , C12N9/2471 , C12Y301/06013 , C12Y302/01023 , C12Y302/01076 , G01N33/573 , G01N2333/924 , G01N2333/928 , G01N2333/938 , G01N2800/38 , G06F19/10
Abstract: A method of preparing a sample for conducting an assay, the method comprising providing an input sample comprising glycoproteins, capturing glycoproteins from the input sample on a solid support, and washing the sample support to remove unbound portions of the input sample.
Abstract translation: 一种制备用于进行测定的样品的方法，所述方法包括提供包含糖蛋白的输入样品，从固体支持物上的输入样品捕获糖蛋白，以及洗涤样品载体以除去输入样品的未结合部分。
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Patent Agency Ranking
公开(公告)号：WO2011126892A3
公开(公告)日：2012-02-23
申请号：PCT/US2011030543
申请日：2011-03-30
Applicant: ADVANCED LIQUID LOGIC INC , SRINIVASAN VIJAY , SUDARSAN ARJUN , BAUER WILLIAM CRAIG , SMITH GREGORY F , POORE MATTHEW , WANG TIH-HONG
Inventor： BAUER WILLIAM CRAIG , POORE MATTHEW , WANG TIH-HONG
CPC classification number: G01N27/44734 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/525 , B01L7/54 , B01L2200/027 , B01L2200/06 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0887 , B01L2300/12 , B01L2300/18 , B01L2300/1805 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , B01L2400/0677 , B01L2400/0683 , G01N27/44791
Abstract: The invention relates to a droplet actuator device and methods for integrated sample preparation and analysis of a biological sample. A droplet actuator device is provided for conducting droplet operations. The droplet actuator device may include a bottom substrate and a top substrate separated from each other to form a gap therebetween; an arrangement of droplet operations electrodes arranged on one or both of the bottom and/or top substrates for conducting droplet operations thereon; a reagent storage layer comprising one or more compartments bound to the top substrate; and one or more openings arranged to provide a fluidic path from the one or more compartments into the gap, upon breach of a breachable seal separating the one or more compartments and openings.
Abstract translation: 本发明涉及一种液滴致动器装置和用于生物样品的综合样品制备和分析的方法。 提供液滴致动器装置用于进行液滴操作。 液滴致动器装置可以包括彼此分离的底部基板和顶部基板，以在它们之间形成间隙; 布置在底部和/或顶部衬底中的一个或两个上的液滴操作电极的布置，用于在其上进行液滴操作; 试剂储存层，包含一个或多个结合到顶部基底的隔室; 以及一个或多个开口，其布置成在违反分离所述一个或多个隔室和开口的可破裂密封件时提供从所述一个或多个隔室进入所述间隙的流体路径。
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公开(公告)号：WO2011002957A2
公开(公告)日：2011-01-06
申请号：PCT/US2010/040705
申请日：2010-07-01
Applicant: ADVANCED LIQUID LOGIC, INC. , POLLACK, Michael G. , SRINIVASAN, Vijay , PAMULA, Vamsee K. , BRACKETT, George , BRAFFORD, Keith R. , CLARK, Darius , HUA, Zhishan , NORTON, Scott , SISTA, Ramakrishna , SMITH, Gregory F. , STURMER, Ryan A. , SUDARSAN, Arjun , THWAR, Prasanna , WANG, Tih-Hong , WINGER, Theodore
Inventor： POLLACK, Michael G. , SRINIVASAN, Vijay , PAMULA, Vamsee K. , BRACKETT, George , BRAFFORD, Keith R. , CLARK, Darius , HUA, Zhishan , NORTON, Scott , SISTA, Ramakrishna , SMITH, Gregory F. , STURMER, Ryan A. , SUDARSAN, Arjun , THWAR, Prasanna , WANG, Tih-Hong , WINGER, Theodore
CPC classification number: B01L3/502707 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/5025 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502784 , B01L3/502792 , B01L2200/0673 , B01L2200/0689 , B01L2300/0819 , B01L2300/0861 , B01L2300/087 , B01L2300/0874 , B01L2300/088 , B01L2300/0883 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0457 , B01L2400/0469 , B01L2400/0622 , B01L2400/0688 , B01L2400/088
Abstract: The invention provides droplet actuator devices for facilitating certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuators that facilitate droplet operations in three dimensions. In another embodiment, the invention provides for cost-effective production of droplet actuators. In another embodiment, the invention provides for replacing one or more components of a droplet actuator. In another embodiment, the invention provides droplet actuators using printed conductive inks to form electrodes and/or ground planes. In another embodiment, the invention provides a magnetic clamping fixture for assembling droplet actuators. In another embodiment, the invention provides simple, low cost power sources for use in combination with microfluidic systems. In another embodiment, the invention provides an immunoassay multiplexing platform that uses digital microfluidics and real-time imaging of flash-based chemiluminescent signals. In still another embodiment, the invention provides droplet actuator devices that are fabricated using a plasma treatment process to raise substrate surface energy.
Abstract translation: 本发明提供了用于促进某些液滴致动分子技术的液滴致动器装置。 在一个实施例中，本发明提供了有助于三维液滴操作的液滴致动器。 在另一个实施例中，本发明提供了液滴致动器的成本有效的生产。 在另一个实施例中，本发明提供了替换液滴致动器的一个或多个部件。 在另一个实施例中，本发明提供使用印刷导电油墨形成电极和/或接地平面的液滴致动器。 在另一个实施例中，本发明提供一种用于组装液滴致动器的磁性夹紧夹具。 在另一个实施例中，本发明提供了用于与微流体系统组合使用的简单的低成本电源。 在另一个实施方案中，本发明提供了使用数字微流体和基于闪光的化学发光信号的实时成像的免疫测定多路复用平台。 在另一个实施例中，本发明提供使用等离子体处理工艺制造以提高衬底表面能的液滴致动器装置。
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公开(公告)号：WO2010019782A3
公开(公告)日：2010-05-14
申请号：PCT/US2009053725
申请日：2009-08-13
Applicant: STURMER RYAN A , SMITH GREGORY F , FOGLEMAN MICHAEL , BRAFFORD KEITH R , RONGALI SAI RAM , ADVANCED LIQUID LOGIC INC
Inventor： STURMER RYAN A , SMITH GREGORY F , FOGLEMAN MICHAEL , BRAFFORD KEITH R , RONGALI SAI RAM
CPC classification number: G06F19/00 , B01F13/0076 , B01L3/502715 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , F04B17/00 , F04B19/006 , F04B49/065
Abstract: The present invention provides modified droplet actuator systems, software, and software-executed methods for use in droplet actuator operation and droplet actuator systems that are configured and programmed to execute such software. An aspect of the software components of the invention is an interface description file for each hardware component of a microfluidics system that allows hardware components to be changed without modifying the program for performing droplet operations protocols. Another aspect of the software components of the invention is the establishment of electrode-to-electrode relationships and other aspects of droplet actuator configurations, which may be used when programming droplet operations protocols. Another aspect of the software components of the invention is a physical design library of predefined electrode elements that may be used by a droplet actuator designer when constructing a layout of electrodes. Another aspect of the software components of the invention is a droplet actuator description file that contains the physical and electrical description of the droplet actuator. Another aspect of the software components of the invention is a router component for determining routes of droplet operations in a droplet actuator. Another aspect of the software components of the invention is the use of tri-state vectors for programming sequences in a droplet actuator. Still other aspects are provided.
Abstract translation: 本发明提供了修改的液滴致动器系统，软件和软件执行的用于液滴致动器操作和液滴致动器系统的方法，其被配置和编程为执行这样的软件。 本发明的软件组件的一个方面是用于微流体系统的每个硬件组件的接口描述文件，该文件允许改变硬件组件，而无需修改用于执行液滴操作协议的程序。 本发明的软件组件的另一个方面是建立微滴执行器配置的电极 - 电极关系和其他方面，其可以在编程微滴操作方案时使用。 本发明的软件组件的另一个方面是预定义的电极元件的物理设计库，其可以在构建电极布局时被液滴致动器设计者使用。 本发明的软件组件的另一方面是包含液滴致动器的物理和电气描述的液滴致动器描述文件。 本发明的软件组件的另一方面是用于确定液滴致动器中的液滴操作路线的路由器组件。 本发明的软件组件的另一个方面是使用三态矢量来在液滴致动器中编程序列。 还提供了其他方面。
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公开(公告)号：WO2010042637A2
公开(公告)日：2010-04-15
申请号：PCT/US2009/059868
申请日：2009-10-07
Applicant: ADVANCED LIQUID LOGIC, INC. , SISTA, Ramakrishna , SUDARSAN, Arjun , PAMULA, Vamsee
Inventor： SISTA, Ramakrishna , SUDARSAN, Arjun , PAMULA, Vamsee
IPC: G01N33/543 , G01N27/00 , C12Q1/68
CPC classification number: G01N33/5308
Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
Abstract translation: 本发明涉及在液滴致动器上的珠子孵育和洗涤。 提供了用一级抗体，样品（即分析物）和二级报道抗体在磁体上，在磁体上和离开磁体并且完全离开磁体来标记磁响应珠的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分裂，运输和分配含有磁性响应珠的样品液滴的方法。 本发明的设备和方法提供了快速的结果和免疫测定中分析物的最佳检测。
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公开(公告)号：WO2010006166A2
公开(公告)日：2010-01-14
申请号：PCT/US2009/050101
申请日：2009-07-09
Applicant: ADVANCED LIQUID LOGIC, INC. , PAMULA, Vamsee , THWAR, Prasanna , SUDARSAN, Arjun , SISTA, Ramakrishna , SULLIVAN, Michael , NORTON, Scott
Inventor： PAMULA, Vamsee , THWAR, Prasanna , SUDARSAN, Arjun , SISTA, Ramakrishna , SULLIVAN, Michael , NORTON, Scott
Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode -mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 液滴可以设置在具有足够强度的磁场的区域内，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式区分或聚集液滴内的珠粒。 该方法还可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 液滴操作在某些情况下可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致靶分析物结合到磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。
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