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    RECIRCULATING FLUIDIC NETWORK AND METHODS FOR USING THE SAME
    71.
    发明申请
    RECIRCULATING FLUIDIC NETWORK AND METHODS FOR USING THE SAME 审中-公开
    循环流体网络及其使用方法

    公开(公告)号：WO2004000721A2

    公开(公告)日：2003-12-31

    申请号：PCT/US2003/019775

    申请日：2003-06-23

    Applicant: FLUIDIGM CORPORATION ,  MANGER, Ian, D. ,  BARCO, Joseph, W. ,  NASSEF, Hany, R.

    Inventor： MANGER, Ian, D. ,  BARCO, Joseph, W. ,  NASSEF, Hany, R.

    IPC: B81C

    CPC classification number: B81B3/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0655 ,  F04B19/006 ,  F04B43/02 ,  F16K99/0001 ,  F16K99/0003 ,  F16K99/0026 ,  F16K99/0036 ,  F16K99/0046 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N33/5304 ,  G01N33/54366 ,  Y10T436/2575

    Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and solid substrate component for attaching ligand(s) on its surface. The elastomeric layer comprises (a) a plurality of first flow channels; (b) a plurality of second flow channels each intersecting and crossing each of said first flow channels thereby providing a plurality of intersecting areas formed at intersections between said first flow channels and said second flow channels, wherein said plurality of first flow channels and said plurality of second flow channels are adapted to allow the flow of a solution therethrough, and wherein said solid substrate surface is in fluid communication with at least said intersecting areas of said plurality of first flow channels and said plurality of second flow channels, and wherein said plurality of first flow channels and/or said plurality of second flow channels are capable of forming a plurality of looped flow channels; (c) a plurality of control channels; (d) a plurality of first control valves each operatively disposed with respect to each of said first flow channel to regulate flow of the solution through said first flow channels, wherein each of said first control valves comprises a first control channel and an elastomeric segment that is deflectable into or retractable from said first flow channel upon which said first control valve operates in response to an actuation force applied to said first control channel, the elastomeric segment when positioned in said first flow channel restricting solution flow therethrough; (e) a plurality of second control valves each operatively disposed with respect to each of said second flow channel to regulate flow of the solution through said second flow channels, wherein each of said second control valves comprises a second control channel and an elastomeric segment that is deflectable into or retractable from said second flow channel upon which said second control valve operates in response to an actuation force applied to said second control channel, the elastomeric segment when positioned in said second flow channel restricting solution flow therethrough; (f) a plurality of loop forming control valves each operatively disposed with respect to each of said first and/or said second flow channels to form said plurality of looped flow channels, wherein each of said loop forming control valves comprises a loop forming control channel and an elastomeric segment that is deflectable into or retractable from said first and/or said second flow channels upon which said loop forming control valve operates in response to an actuation force applied to said loop forming control channel, the elastomeric segment when positioned in said first and/or said second flow channels restricting solution flow therethrough thereby forming said looped flow channel; and (g) a plurality of recirculating pumps, and wherein each recirculating pump is operatively disposed with respect to one of said looped flow channels such that circulation of solution through each of said looped flow channels can be regulated by one of said recirculating pumps.

    Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括用于在其表面上附着配体的弹性体组分和固体底物组分。 弹性体层包括（a）多个第一流动通道; （b）多个第二流动通道，每个第二流动通道与每个所述第一流动通道相交并交叉，从而提供在所述第一流动通道和所述第二流动通道之间的交叉处形成的多个相交区域，其中所述多个第一流动通道和所述多个 第二流动通道适于允许溶液流过其中，并且其中所述固体基底表面与所述多个第一流动通道和所述多个第二流动通道中的至少所述相交区域流体连通，并且其中所述多个 的第一流动通道和/或所述多个第二流动通道能够形成多个环形流动通道; （c）多个控制通道; （d）多个第一控制阀，每个第一控制阀相对于每个所述第一流动通道操作地设置，以调节溶液通过所述第一流动通道的流动，其中每个所述第一控制阀包括第一控制通道和弹性体段， 响应于施加到所述第一控制通道的致动力，所述弹性段在所述第一流动通道中被限制溶液流动通过其中时，可偏转到所述第一流动通道中或从所述第一流动通道缩回。 （e）多个第二控制阀，每个第二控制阀相对于每个所述第二流动通道操作地设置以调节溶液通过所述第二流动通道的流动，其中每个所述第二控制阀包括第二控制通道和弹性体段， 响应于施加到所述第二控制通道的致动力，所述弹性体段定位在所述第二流动通道中以限制溶液流过其中时，可偏转到所述第二流动通道中或从所述第二流动通道缩回所述第二控制阀。 （f）多个回路成形控制阀，每个操作地相对于每个所述第一和/或所述第二流动通道设置，以形成所述多个环形流动通道，其中每个所述回路成形控制阀包括回路成形控制通道 以及弹性体段，其可偏转到所述第一和/或所述第二流动通道中或从所述第二和/或所述第二流动通道中缩回，所述环形成控制阀响应于施加到所述环形成控制通道的致动力而操作，所述弹性体段在定位在所述第一 和/或所述第二流动通道限制溶液流过其中从而形成所述环形流动通道; 和（g）多个循环泵，并且其中每个循环泵相对于所述环形流动通道中的一个可操作地设置，使得循环

    MICROFABRICATED FLUIDIC CIRCUIT ELEMENTS AND APPLICATIONS
    72.
    发明申请
    MICROFABRICATED FLUIDIC CIRCUIT ELEMENTS AND APPLICATIONS 审中-公开

    公开(公告)号：WO2002081935A3

    公开(公告)日：2002-10-17

    申请号：PCT/US2001/045294

    申请日：2001-11-30

    Applicant: FLUIDIGM CORPORATION ,  FERNANDES, David, N. ,  CHOU, Hou-Pu ,  UNGER, Marc, A.

    Inventor： FERNANDES, David, N. ,  CHOU, Hou-Pu ,  UNGER, Marc, A.

    IPC: F15C5/00

    Abstract: The present invention provides microfabricated fluidic systems and methods. Microfabricated fluidic devices of the present invention include switches (232, 233) that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure (P2) in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include pressure sources (560). A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves (500). The pressure source may be high pressure source or a low pressure source. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.

    LASER CAPTURE MICRODISSECTION APPARATUS, SYSTEM AND METHOD
    76.
    发明申请
    LASER CAPTURE MICRODISSECTION APPARATUS, SYSTEM AND METHOD 有权

    公开(公告)号：US20220413277A1

    公开(公告)日：2022-12-29

    申请号：US17777894

    申请日：2020-11-17

    Applicant: Fluidigm Corporation

    Inventor： Daaf Sandkuijl ,  Alan Carpino

    IPC: G02B21/16 ,  G02B21/32

    Abstract: A microscopy apparatus comprises a microscope comprising a stage configured to hold a tissue sample, a UV laser assembly configured to emit a UV laser beam to a viewing area of the tissue sample, and an IR laser assembly configured to emit an IR laser beam to the viewing area of the tissue sample. The UV and IR laser assemblies are oriented so as to emit the respective UV and IR laser beams in a same direction.

    Methods and kits for extracting nucleic acids from paraffin embedded samples
    77.
    发明授权
    Methods and kits for extracting nucleic acids from paraffin embedded samples 有权

    公开(公告)号：US11078476B2

    公开(公告)日：2021-08-03

    申请号：US16105711

    申请日：2018-08-20

    Applicant: Fluidigm Corporation

    Inventor： Jian Qin ,  Peilin Chen ,  Ramesh Ramakrishnan

    IPC: C12N15/10

    Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES
    80.
    发明申请
    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES 审中-公开

    公开(公告)号：US20190055540A1

    公开(公告)日：2019-02-21

    申请号：US16105711

    申请日：2018-08-20

    Applicant: Fluidigm Corporation

    Inventor： Jian Qin ,  Peilin Chen ,  Ramesh Ramakrishnan

    IPC: C12N15/10

    Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

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