公开(公告)号：US20220056184A1

公开(公告)日：2022-02-24

申请号：US17460058

申请日：2021-08-27

Applicant: Life Technologies AS

Inventor： Geir Fonnum ,  Nils Pontus Lundberg ,  Muhammed Talha Gokmen ,  Synne Larsen ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth Schmid ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US11041068B2

公开(公告)日：2021-06-22

申请号：US16305372

申请日：2017-06-07

Applicant: LIFE TECHNOLOGIES AS ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Kristian Andersson ,  Geir Fonnum ,  Korbinian Heil

IPC: C08L25/14 ,  C08L25/08 ,  B01J19/00 ,  C08F212/08 ,  C08F220/58 ,  C08F293/00

Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.

公开(公告)号：US20210155726A1

公开(公告)日：2021-05-27

申请号：US17165039

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofsloekken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem Thuy Thi TRAN ,  Jo AASERUD ,  M. Tahla GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C07F7/18 ,  C08F8/12 ,  C12Q1/686 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F6/24 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20210139626A1

公开(公告)日：2021-05-13

申请号：US17066326

申请日：2020-10-08

Applicant: Life Technologies AS ,  SINTEF

Inventor： Geir FONNUM ,  Nils Pontus LUNDBERG ,  Muhammed Talha GOKMEN ,  Synne LARSEN ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth SCHMID ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US10947333B2

公开(公告)日：2021-03-16

申请号：US16259963

申请日：2019-01-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  Muhammed Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F230/08 ,  C08F220/58 ,  C08F8/34 ,  C08F8/32 ,  C08F8/12 ,  C08F2/20 ,  C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C12Q1/686 ,  C08F2/26 ,  C08F222/38 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20200308630A1

公开(公告)日：2020-10-01

申请号：US16849833

申请日：2020-04-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/6806 ,  C12N15/10

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US10787659B2

公开(公告)日：2020-09-29

申请号：US16137448

申请日：2018-09-20

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Hannah Lindstroem

IPC: C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.

公开(公告)号：US20200277595A1

公开(公告)日：2020-09-03

申请号：US16645896

申请日：2018-08-30

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT

IPC: C12N15/10

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

公开(公告)号：US20190390018A1

公开(公告)日：2019-12-26

申请号：US16253141

申请日：2019-01-21

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene HUSABOE ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F220/70 ,  C08F8/12 ,  C08F220/58

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20190177787A1

公开(公告)日：2019-06-13

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  Alfred LUI ,  Guobing XIANG ,  Synne LARSEN

IPC: C12Q1/6874 ,  C08J7/14 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.