公开(公告)号：EP2288696A2

公开(公告)日：2011-03-02

申请号：EP09743681.0

申请日：2009-05-07

Applicant: Pressure Biosciences, Inc.

Inventor： TAO, Feng ,  SCHUMACHER, Richard ,  LAWRENCE, Nathan ,  GROSS, Vera ,  SMEJKAL, Gary ,  LAZAREV, Alexander ,  HAN, Rui

IPC: C12N9/20 ,  C12N9/24 ,  C12N9/50 ,  C12N5/06

CPC classification number: C12N1/066 ,  C12N13/00

Abstract: Extraction methods that allow a molecular complex (e.g., an organelle) to be extracted from a sample by employing pressure cycling are described.

公开(公告)号：EP1390466A4

公开(公告)日：2010-07-28

申请号：EP02728998

申请日：2002-04-26

Applicant: PRESSURE BIOSCIENCES INC

Inventor： SCHUMACHER RICHARD T ,  TAO FENG ,  LAWRENCE NATHAN P ,  KAKITA ALLAN ,  MANAK MARK M ,  LAUGHARN JAMES A JR

IPC: G01N33/53 ,  B01L3/00 ,  C12M1/33 ,  C12N15/00 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N33/566 ,  C12M1/12

CPC classification number: B01L3/502 ,  B01L2200/026 ,  B01L2300/044 ,  B01L2300/0663 ,  B01L2300/0832 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0677 ,  B01L2400/0683 ,  G01N1/286 ,  G01N1/4077 ,  G01N2001/2866 ,  Y10T436/25375

公开(公告)号：EP2600843B9

公开(公告)日：2020-03-11

申请号：EP11810258.1

申请日：2011-07-19

Applicant: Pressure BioSciences, Inc. ,  The Regents of the University of Colorado, A Body Corporate

Inventor： SEEFELDT, Matthew ,  RANDOLPH, Theodore W. ,  FRADKIN, Amber Haynes ,  CARPENTER, John

IPC: A61K9/16 ,  A61K9/50 ,  A61K38/17 ,  C07K16/24 ,  A61K38/27 ,  C07K1/113

公开(公告)号：EP2329246A2

公开(公告)日：2011-06-08

申请号：EP09760361.7

申请日：2009-09-17

Applicant: Pressure Biosciences, Inc.

Inventor： TING, Edmund, Y. ,  LAZAREV, Alexander ,  GROSS, Vera ,  DUSSAULT, Charles ,  LI, Chunqin ,  LAWRENCE, Nathan ,  SCHUMACHER, Richard, T.

IPC: G01N1/28

CPC classification number: G01N1/286 ,  C12M35/04 ,  G01N2001/2866

Abstract: The systems and techniques of the present invention can also synergistically utilize mechanical disruption processes with the use of high hydrostatic pressure extraction, such as pressure cycling extraction techniques to achieve high yield of difficult to extract sample constituents without generating high shear stress or high temperatures.

公开(公告)号：EP4037988A1

公开(公告)日：2022-08-10

申请号：EP20872810.5

申请日：2020-09-30

Applicant: Pressure Biosciences, Inc.

Inventor： TING, Edmund, Y. ,  LAZAREV, Alexander

IPC: B65D47/06 ,  A47G19/22 ,  A61B1/00

公开(公告)号：EP2785196B1

公开(公告)日：2020-08-05

申请号：EP12854533.2

申请日：2012-12-03

Applicant: Pressure BioSciences, Inc.

Inventor： SEEFELDT, Matthew ,  GOMEZ, Eliana

IPC: A23J1/00 ,  C07K1/00 ,  C12P21/06 ,  C07K1/14 ,  C07K14/555 ,  C07K14/54 ,  C07K1/113 ,  C12P21/02 ,  C12N9/50

公开(公告)号：EP3149151A1

公开(公告)日：2017-04-05

申请号：EP15798802.3

申请日：2015-05-29

Applicant: Pressure Biosciences, Inc.

Inventor： GROSS, Vera ,  TING, Edmund, Y. ,  LAZAREV, Alexander ,  SCHUMACHER, Richard, T.

IPC: C12N1/06 ,  C12M3/08 ,  G01N1/28

CPC classification number: B01L3/50853 ,  B01L3/5082 ,  B01L2300/042 ,  B01L2300/046 ,  G01N1/286

Abstract: Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.

Abstract translation: 公开了通过压力循环技术进行样品制备的装置和方法。 用于样品制备的装置包括具有内表面，顶部和底部的管，所述管被配置为在底部容纳样品并被接收到用于样品制备的压力室中，所述管可拆卸地连接到 所述管和从所述盖延伸到所述管中的锥形细长构件，所述锥形细长构件构造成接触所述管的内表面和所述管的底部中的样品，其中所述管是可变形的，使得在压力下操作 管对锥形细长构件变形以促进样品的破坏。

公开(公告)号：EP2155351A4

公开(公告)日：2011-12-21

申请号：EP08769978

申请日：2008-06-02

Applicant: PRESSURE BIOSCIENCES INC

Inventor： LAZAREV ALEXANDER ,  GROSS VERA

IPC: B01D21/01 ,  C07K1/14

CPC classification number: C07K1/145

公开(公告)号：EP1027160B1

公开(公告)日：2010-12-22

申请号：EP98956405.9

申请日：1998-10-30

Applicant: Pressure Biosciences, Inc.

Inventor： LAUGHARN, James, A., Jr. ,  HESS, Robert, A. ,  TAO, Feng

IPC: B01L99/00 ,  B32B27/04 ,  C07H1/00 ,  G01N1/14 ,  G01N27/447 ,  B01L3/00

CPC classification number: G01N30/00 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  C12M47/06 ,  C12M47/12 ,  C12N1/06 ,  C12N15/101 ,  G01N27/44704 ,  G01N30/02 ,  G01N30/14 ,  G01N30/52 ,  G01N30/6047 ,  G01N2030/009 ,  G01N2030/285 ,  G01N2030/522 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2030/8827

Abstract: Methods for cell lysis and purification of biological materials, involving subjecting a sample to high pressure. Also featured is an apparatus for practicing the methods.

公开(公告)号：EP2129456A2

公开(公告)日：2009-12-09

申请号：EP08728313.1

申请日：2008-01-25

Applicant: Pressure Biosciences Inc.

Inventor： TING, Edmund, Y.

IPC: B01J3/00 ,  B01J3/03 ,  C12M1/00

CPC classification number: B01J3/00 ,  B01J3/03 ,  B01J2219/00065 ,  B01J2219/00162 ,  B01J2219/002 ,  B01J2219/00213 ,  B01J2219/00231

Abstract: A pressure cycling system includes a reaction chamber configured to receive a sample and a charge pump in fluid communication with the reaction chamber. The charge pump is operable to convey a fluid from a fluid source toward the reaction chamber. The system also includes a check valve disposed between the charge pump and the reaction chamber. The check valve is operable to inhibit the flow of fluid from the reaction chamber toward the charge pump. A pressure intensifier is in fluid communication with the reaction chamber. The pressure intensifier is pneumatically operable to adjust a pressure in the reaction chamber. A controller is configured to control operation of the charge pump and the pressure intensifier. The controller is configured to pressurize the reaction chamber to a first pressure through operation of the charge pump. The controller is also configured to fluctuate the pressure in the reaction chamber between a second pressure and a third pressure through operation of the pressure intensifier.

Abstract translation: 压力循环系统包括构造成接收样本的反应室和与反应室流体连通的电荷泵。 电荷泵可操作以将流体从流体源传送到反应室。 该系统还包括设置在电荷泵和反应室之间的止回阀。 止回阀可操作以阻止流体从反应室流向电荷泵。 增压器与反应室流体连通。 增压器可气动地操作以调节反应室中的压力。 控制器被配置为控制电荷泵和增压器的操作。 控制器构造成通过操作电荷泵将反应室加压至第一压力。 控制器还被配置为通过增压器的操作使反应室中的压力在第二压力和第三压力之间波动。