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    HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST
    71.
    发明公开
    HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST 审中-公开
    HEFE中的异位表达合成酶PILZPOLYKETIDGENE

    公开(公告)号：EP2610339A1

    公开(公告)日：2013-07-03

    申请号：EP11816242.9

    申请日：2011-08-11

    Applicant: Shizuoka Prefecture Public University Corporation ,  National University Corporation Okayama University

    Inventor： WATANABE, Kenji ,  MORIYA, Hisao

    IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

    CPC classification number: C12P19/34 ,  C12N15/52 ,  C12N15/80 ,  C12N15/815 ,  C12P17/06 ,  C12P21/02

    Abstract: The present invention relates to a method of removing an intron contained in a gene from a eukaryotic gene, and linking only the exon sequences to prepare an expression vector comprising the linked sequences. Specifically, the invention relates to a method of preparing an expression vector containing linked exon sequences comprising amplifying exon sequences by PCR as one or more fragments from a giant fungal gene containing an intron, and linking the fragments together with a restriction enzyme-treated vector using the gap repair cloning method; a method of preparing an expression vector containing a full-length cDNA sequence by synthesizing and linking cDNA fragments from a fungal giant gene; a transformant having introduced therein an expression vector prepared by the method; a protein produced by the transformant; and a method of preparing a compound produced by the protein using the expression vector.

    Abstract translation: 本发明涉及从真核基因中除去包含在基因中的内含子的方法，并且仅连接外显子序列以制备包含连接序列的表达载体。 具体地，本发明涉及一种制备含有连锁外显子序列的表达载体的方法，其包括通过PCR扩增外显子序列作为来自含有内含子的巨型真菌基因的一个或多个片段，并将所述片段与限制酶处理的载体连接，使用 间隙修复克隆方法; 通过合成和连接来自真菌巨基因的cDNA片段来制备含有全长cDNA序列的表达载体的方法; 在其中引入了通过该方法制备的表达载体的转化体; 由转化体产生的蛋白质; 以及使用表达载体制备由蛋白质产生的化合物的方法。

    PAPER STRING HAVING WATER RESISTANCE AND SUPPORTING TOOL FOR CULTIVATING PLANT USING THE SAME
    73.
    发明专利
    PAPER STRING HAVING WATER RESISTANCE AND SUPPORTING TOOL FOR CULTIVATING PLANT USING THE SAME 失效

    公开(公告)号：JP2000032852A

    公开(公告)日：2000-02-02

    申请号：JP20261498

    申请日：1998-07-17

    Applicant: MISHIMA PAPER CO LTD ,  SHIZUOKA PREFECTURE ,  SHIZUOKA PREFECTURE KAGAKU GIJ

    Inventor： ISHINO YOSHIAKI ,  HATTORI YORIYUKI ,  IKEGAMI GENICHI ,  HIYOSHI KIMIO ,  FUKAZAWA HIROYUKI ,  ENDO YASUNOBU ,  YAMASHITA RIE

    IPC: A01G9/12 ,  D21H17/00

    Abstract: PROBLEM TO BE SOLVED: To obtain a paper string excellent in water and weather resistances and biodegradability and suitable for cultivating a plant without requiring removing and recovering operations and to provide a netlike supporting tool for cultivating the plant producible by knitting in or heat-seal joining using the paper string and excellent in the water and weather resistances and biodegradability without requiring the removing and recovering operations. SOLUTION: This paper string having water resistance is obtained by twisting a blend paper of biodegradable thermoplastic synthetic staple fibers comprising 20-90 wt.% of the biodegradable thermoplastic synthetic staple fibers and 10-80 wt.% of vegetable fibers for papermaking and having 15-80 g/m2 basis weight or a combination paper prepared by joining a layer containing the biodegradable thermoplastic synthetic staple fibers to at least one surface of a layer without containing the biodegradable thermoplastic synthetic staple fibers according to the combination and having 15-80 g/m2 basis weight. Furthermore, the netlike supporting tool for cultivating a plant is obtained by weaving the paper string or crossing the paper string without weaving and thermally fusing the interlaced parts or knitting the paper string therein.

    BIODEGRADABLE BAG PAPER
    74.
    发明专利
    BIODEGRADABLE BAG PAPER 失效

    公开(公告)号：JPH11138728A

    公开(公告)日：1999-05-25

    申请号：JP30835097

    申请日：1997-11-11

    Applicant: MISHIMA PAPER CO LTD ,  SHIZUOKA PREFECTURE ,  SHIZUOKA PREFECTURE KAGAKU GIJ

    Inventor： KIMURA YUJI ,  ISHINO YOSHIAKI ,  HATSUTORI YORIYUKI ,  IKEGAMI GENICHI ,  HIYOSHI KIMIO ,  FUKAZAWA HIROYUKI

    IPC: B32B29/00 ,  C08L101/16 ,  D21H13/24

    Abstract: PROBLEM TO BE SOLVED: To use biodegradable bag paper as a material for an industrial bag capable of being made by heat sealing and being degradated with microorganism, by a method wherein a layer capable of being heat sealed which contains a specific wt.% of biodegradable thermoplastic synthetic short fiber, and a layer incapable of being heat sealed which consists of natural fiber for making paper are bonded to each other by making paper together. SOLUTION: Biodegradable bag paper is manufactured by making paper together from a layer containing biodegradable thermoplastic synthetic short fiber and a layer consisting of natural fiber for making paper. The short fiber consists of a polycondensate of fatty glycol and fatty dicarboxylic acid, or biodegradable thermoplastic resin synthetic resin of polylactate series copolymer such as lactic acid and glycollide copolymer. A heat seal layer 2 capable of being heat sealed in which 30 to 90 wt.% of the short fiber is blended, and a non-heat seal layer 1 incapable of being heat sealed which consists of natural fiber for making paper are formed as paper made from two layers capable of being degradated with microorganism or as paper made from three layers wherein the non-heat seal layer 1 is made its intermediate layer by a paper-making method in a paper-making technique.

    METHOD AND KIT FOR DISTINGUISHING BETWEEN PROSTATE CARCINOMA AND BENIGN PROSTATIC HYPERPLASIA
    77.
    发明公开
    METHOD AND KIT FOR DISTINGUISHING BETWEEN PROSTATE CARCINOMA AND BENIGN PROSTATIC HYPERPLASIA 审中-公开
    VERFAHREN UND KIT ZUR UNTERSCHEIDUNG ZWISCHEN EINEM PROSTATAKARZINOM UND BENIGNER PROSTATAHYPERPLASIE

    公开(公告)号：EP2908136A1

    公开(公告)日：2015-08-19

    申请号：EP13846084.5

    申请日：2013-10-09

    Applicant: Hirosaki University ,  Shizuoka Prefecture Public University Corporation

    Inventor： OHYAMA, Chikara ,  YONEYAMA, Tohru ,  TOBISAWA, Yuki ,  HATAKEYAMA, Shingo ,  SUZUKI, Takashi ,  JWA, Ilpal ,  YAMAGUCHI, Maho

    IPC: G01N33/574 ,  G01N33/553

    CPC classification number: G01N33/57434 ,  G01N2333/96433 ,  G01N2400/02 ,  G01N2400/12

    Abstract: An object of the present invention is to provide a method for distinguishing between prostate carcinoma and benign prostatic hyperplasia with high sensitivity and good reproducibility using a small amount of an analyte sample. The method for distinguishing between prostate carcinoma and benign prostatic hyperplasia according to the present invention as a solution means thereof comprises: bringing an analyte sample containing a prostate-specific antigen (PSA) into contact with a carrier having an anti-free PSA antibody immobilized thereon, thereby binding free PSA to the anti-free PSA antibody immobilized on the carrier; thereafter bringing the carrier in which the free PSA is bound to the immobilized anti-free PSA antibody into contact with a monoclonal antibody capable of specifically recognizing a glycan in which a terminal sialic acid residue is bound to galactose through an α(2,3) bond, thereby binding the monoclonal antibody capable of specifically recognizing a glycan in which a terminal sialic acid residue is bound to galactose through an α(2, 3) bond to the free PSA bound to the anti-free PSA antibody immobilized on the carrier; measuring the amount of the free PSA having an N-type glycan in which a terminal sialic acid residue is bound to galactose through an α(2, 3) bond; comparing the measured amount thus obtained with a preset cutoff value for prostate carcinoma and benign prostatic hyperplasia, thereby determining that when the measured amount is larger than the cutoff value, prostate carcinoma is developed or the probability of developing prostate carcinoma is high, and when the measured amount is smaller than the cutoff value, benign prostatic hyperplasia is developed or the probability of developing benign prostatic hyperplasia is high.

    Abstract translation: 本发明的目的是提供使用少量分析物样品，以高灵敏度和良好的再现性来区分前列腺癌和良性前列腺增生的方法。 根据本发明的前列腺癌和良性前列腺增生的区分方法作为其溶液方法包括：将含有前列腺特异性抗原（PSA）的分析物样品与其上固定有抗PSA抗体抗体的载体接触 从而将游离PSA结合到固定在载体上的抗自由PSA抗体; 然后将游离PSA结合的载体与固定的抗自由PSA抗体接触，与能够特异性识别末端唾液酸残基与半乳糖结合的聚糖的单克隆抗体通过±（2,3） 从而将能够特异性识别末端唾液酸残基与半乳糖结合的聚糖的单克隆抗体通过±（2,3）键结合到与固定在载体上的抗自由PSA抗体结合的游离PSA; 测量其中末端唾液酸残基通过±（2,3）键与半乳糖结合的N-型聚糖的游离PSA的量; 将由此获得的测量量与前列腺癌和良性前列腺增生的预设截止值进行比较，从而确定当测量量大于截止值时，前列腺癌发展或发展为前列腺癌的概率高，并且当 测量量小于临界值，发展良性前列腺增生或发展良性前列腺增生的可能性高。

    METHOD FOR ANALYSIS/IDENTIFICATION OF ANTIBODY GENE AT ONE-CELL LEVEL
    79.
    发明公开
    METHOD FOR ANALYSIS/IDENTIFICATION OF ANTIBODY GENE AT ONE-CELL LEVEL 有权
    VERFAHREN ZUR ANALYZE / IDENTIFIZIERUNG EINESANTIKÖRPERGENSAUF EINZELZELLEBENE

    公开(公告)号：EP2316936A1

    公开(公告)日：2011-05-04

    申请号：EP09758131.8

    申请日：2009-06-04

    Applicant: Shizuoka Prefecture

    Inventor： AKIYAMA, Yasuto

    IPC: C12N15/09 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68

    CPC classification number: G01N33/56972 ,  G01N33/6854

    Abstract: Disclosed are: a method for identifying/analyzing a gene for an antibody in one cell derived from a human; a technique for producing an antibody derived from an identified one B cell; and others. A gene for an antibody specific to a melanoma antigen is analyzed/identified at a one-cell level by using an immortalized B cell produced from peripheral blood monocytes from a melanoma patient or a primary B cell included in the peripheral blood monocytes. It is found that B cells capable of producing a specific antibody can be separated on one cell by one cell basis by staining the B cells with a GST-labeled melanoma-specific cancer antigen MAGE1, an Alexa-labeled anti-GST antibody and a PE-labeled anti-human IgG antibody and carrying out the single cell sorting of the stained B cells. Further, a practical technique for extracting total RNA from the separated one B cell and cloning a gene for a specific antibody into the total RNA efficiently can be established.

    Abstract translation: 公开了一种用于鉴定/分析来源于人的一种细胞中的抗体基因的方法; 用于产生源自鉴定的一种B细胞的抗体的技术; 和别的。 通过使用由来自黑素瘤患者的外周血单核细胞或包含在外周血单核细胞中的原代B细胞产生的永生化B细胞，在单细胞水平分析/鉴定用于黑素瘤抗原特异性抗体的基因。 发现能够产生特异性抗体的B细胞可以通过用GST标记的黑素瘤特异性癌抗原MAGE1，Alexa标记的抗GST抗体和PE染色B细胞，在一个细胞上通过一个细胞分离 标记的抗人IgG抗体，并进行染色的B细胞的单细胞分选。 此外，可以建立从分离的一个B细胞中提取总RNA并将特异性抗体的基因有效克隆到总RNA中的实用技术。

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