公开(公告)号：KR100573466B1

公开(公告)日：2006-05-02

申请号：KR1020040076364

申请日：2004-09-23

Applicant: 대한민국(농촌진흥청장) ,  알보젠코리아 주식회사

Inventor： 강필돈 ,  김진원 ,  김미자 ,  강석우 ,  류강선 ,  박용수 ,  권무길 ,  이민주 ,  서성곤 ,  문석식 ,  조순장 ,  송준식

IPC: A23L33/00 ,  A61K35/64

Abstract: 본 발명은 누에번데기 추출, 분리 및 정제에 관한 방법으로써, 이를 건강보조식품, 의약품 또는 화장품 개발에 이용하고자 함이다.
누에번데기추출물, 리놀산, 리놀렌산.

公开(公告)号：KR100453574B1

公开(公告)日：2004-10-20

申请号：KR1020010045133

申请日：2001-07-26

Applicant: 대한민국(농촌진흥청장)

Inventor： 안미영 ,  류강선 ,  이완주 ,  김영식 ,  이용기

IPC: A61K35/64 ,  A61K38/46 ,  A23L1/30

Abstract: PURPOSE: A composition containing new serine protease having thrombolysis obtained by extraction and purification of Scarabaeidae as an insect is provided which is excellent in fibrinolytic activity and can be used as a therapeutic agent for treatment of diseases related with thrombus. CONSTITUTION: Scarabaeidae as an insect is soaked in a mixed solution of tris-hydrochloric buffer solutions and sodium chloride solutions, centrifuged and purified after extraction to produce serine protease. The serine protease has an amino acid sequence represented by an amino acid sequence represented by SEQ ID NO:1 or 2 in Sequence Listing at the amino terminus, a molecular weight of 27,000 daltons and optimum activity in the range of 10 to 15deg.C and pH 5.0 to 10.0.

Abstract translation: 目的：提供一种组合物，其含有通过提取和纯化作为昆虫的金龟子科动物而获得的具有血栓溶解作用的新的丝氨酸蛋白酶，其具有优异的纤维蛋白溶解活性并且可用作治疗与血栓相关的疾病的治疗剂。 组成：作为昆虫的金龟子科被浸泡在三盐酸缓冲溶液和氯化钠溶液的混合溶液中，提取后离心和纯化以产生丝氨酸蛋白酶。 丝氨酸蛋白酶在氨基末端具有由序列表中SEQ ID NO：1或2表示的氨基酸序列表示的氨基酸序列，分子量为27,000道尔顿，最佳活性为10至15℃，和 pH 5.0至10.0。

公开(公告)号：KR1020030005516A

公开(公告)日：2003-01-23

申请号：KR1020010040836

申请日：2001-07-09

Applicant: 대한민국(농촌진흥청장)

Inventor： 안미영 ,  류강선 ,  이용우 ,  김영식 ,  이용기 ,  이희삼 ,  김은선 ,  김익수

IPC: A23L1/30

CPC classification number: A23L33/10

Abstract: PURPOSE: A gryllidae extract having a liver protecting effect and alcohol detoxifying and antifatigue properties is provided. The product is a natural insect extract, it is thus effectively used as an agent for treatment of liver diseases and a tonic supplement food product. CONSTITUTION: A cricket(gryllidae) is freeze-dried and extracted in distilled water or methanol(ethanol), the extract is used in a food and a drug for improving liver function, promoting recovery from fatigue, improving a tonic effect, increasing alcoholysis(preventing hangover) and treating antioxidation related diseases(aging or dementia).

Abstract translation: 目的：提供具有肝脏保护作用和酒精解毒和抗疲劳特性的id科提取物。 该产品是天然昆虫提取物，因此有效地用作治疗肝脏疾病和滋补补品食品的药剂。 构成：将蟋蟀（gryllidae）冷冻干燥并在蒸馏水或甲醇（乙醇）中提取，提取物用于食品和药物，用于改善肝功能，促进疲劳恢复，提高补品效果，增加醇解（ 预防宿醉）和治疗抗氧化相关疾病（老化或痴呆）。

公开(公告)号：KR1020020071334A

公开(公告)日：2002-09-12

申请号：KR1020010011449

申请日：2001-03-06

Applicant: 대한민국(농촌진흥청장) ,  사회복지법인 삼성생명공익재단

Inventor： 이희삼 ,  류강선 ,  김진원 ,  김익수 ,  이용기 ,  안미영 ,  김은선 ,  김광원 ,  서수원

IPC: C07D211/14

Abstract: PURPOSE: Provided is a process for preparing α-glycosidase inhibitor derived from powdery silkworm, which has the same decreasing effect of blood glucose level as acarbose which is a conventional therapeutic agent for diabetes. CONSTITUTION: The process for preparing α-glycosidase inhibitor comprises the steps of: freeze-drying and powdering silkworm then adding 50% ethanol to extract it; performing cation exchange column chromatography with the extract then followed by eluting it with 0.5N of NH4OH and concentrating it; dissolving the concentrate in water then followed by performing anion exchange column chromatography. eluting with water and concentrating it; dissolving the concentrate in water then followed by cation exchange column chromatography, eluting with water and 0.5N of NH4OH to obtain 5 fractions obtain one fraction respectively; and performing anion exchange column chromatography with the second fraction among the 6 fractions to obtain pure 1-dioxynojirimycin fraction.

Abstract translation: 目的：提供一种从粉状蚕制备α-糖苷酶抑制剂的方法，其与糖尿病常规治疗剂阿卡波糖具有相同的血糖水平降低作用。 构成：制备α-糖苷酶抑制剂的方法包括以下步骤：冷冻干燥并粉化蚕，然后加入50％乙醇提取; 用提取物进行阳离子交换柱色谱，然后用0.5N NH 4 OH洗脱并浓缩; 将浓缩物溶解在水中，然后进行阴离子交换柱层析。 用水洗脱并浓缩; 将浓缩物溶解在水中，然后进行阳离子交换柱层析，用水和0.5N的NH 4 OH洗脱，得到5份，分别得到1份; 并用6份馏分中的第二级分进行阴离子交换柱层析，得到纯的1-二氧基野尻霉素级分。

公开(公告)号：KR100286388B1

公开(公告)日：2001-05-02

申请号：KR1019980008600

申请日：1998-03-14

Applicant: 대한민국(농촌진흥청장)

Inventor： 이용우 ,  이광길 ,  김선여 ,  여주홍 ,  류강선 ,  임수호

IPC: C12P21/06

Abstract: PURPOSE: Provided is a method for preparing silk power peptide by enzymatic degradation of silk fibroin. The peptide has a molecular weight of 12,000-13,000, polymerization of 163 and solubility of 70% at pH 1-13, and its internal absorptivity is thus high, so as to contribute to development of functional foods or medicines. CONSTITUTION: The method for preparing silk power peptide by enzymatic degradation of silk fibroin comprises the following steps of: (a) purifying silk fibroin by adding flavourzyme or a protein produced from Aspergillus oryzae and Bacillus subtilis, Bacillus thermoproteolyticus Rokko thereinto; (b) removing salt by adding calcium chloride and ethanol in the resultant and dialyzing them; and (c) obtaining powdered peptide by adding flavourzyme or a protein produced from Aspergillus oryzae and Bacillus subtilis, Bacillus thermoproteolyticus Rokko into the solution and then reacting and freeze-drying them.

公开(公告)号：KR100260612B1

公开(公告)日：2000-07-01

申请号：KR1019970074866

申请日：1997-12-27

Applicant: 대한민국(농촌진흥청장) ,  대원실업주식회사 ,  주식회사 녹십자

Inventor： 정성현 ,  류강선 ,  한만영 ,  이희삼 ,  안기포 ,  강필돈 ,  송주경

IPC: A61K31/045

Abstract: PURPOSE: An α-glycosidase inhibition material from silkworm urine is provided, which shows nearly same α-glycosidase inhibition effect as the existing diabetes medicine, the Acarbose, thereby contributing to increase of farm income and enhancement of national health. CONSTITUTION: A process for the preparation of α-glycosidase inhibition material from silkworm urine comprises the steps of: filtering the silkworm urine, freeze-drying to get powder, and adding methanol(alcohol-precipitation); processing anion-exchange column chromatography with the methanol-soluble part to get active part; separating into methanol-soluble part and insoluble part to remove inorganic material in silkworm urine; adding water and acetonitrile to methanol-soluble part to separate into acetonitrile layer and water layer, and concentrating the water layer and separating into A, B and C fractions(1-deoxynojirimycin, fagomine, 1,4-dideoxy-1,4-imino-D-araviniton showing excellent α-glycosidase inhibition effect).

Abstract translation: 目的：提供蚕尿中的α-糖苷酶抑制物质，与现有糖尿病药物阿卡波糖显示几乎相同的α-糖苷酶抑制作用，从而有助于增加农民收入，提高国民健康水平。 构成：从蚕尿制备α-糖苷酶抑制物质的方法包括以下步骤：过滤蚕尿，冷冻干燥得到粉末，并加入甲醇（酒精沉淀）; 用甲醇溶解部分处理阴离子交换柱层析，得到活性部分; 分离成甲醇可溶部分和不溶部分，去除蚕尿中的无机物质; 将水和乙腈加入到可溶于甲醇的部分，分离成乙腈层和水层，浓缩水层并分离成A，B和C级分（1-脱氧野尻霉素，fagomine，1,4-二脱氧-1,4-亚氨基 -D-araviniton显示出优异的α-糖苷酶抑制作用）。

公开(公告)号：KR100151731B1

公开(公告)日：1998-10-15

申请号：KR1019950001068

申请日：1995-01-23

Applicant: 대한민국(농촌진흥청장)

Inventor： 류강선 ,  정성현 ,  홍기원 ,  이상풍

IPC: A61K35/64 ,  A61K9/14 ,  A23L1/30

Abstract: 본 발명은 누에분말을 유효성분으로 포함하는 혈당강하제 및 그의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 누에를 질소가스로 산화방지 처리 및 동결건조하여 분말화하는 공정을 포함하는 누에분말의 제조방법과 그를 유효성분으로 포함하는 혈당강하제 및 건강보조식품에 관한 것이다. 본 발명에서는 4령기잠 내지 5령말 극대중 누에를 질소가스를 이용하여 산화방지처리 및 동결건조하여 전기혈당강하제의 유효성분인 누에분말을 제조한다. 본 발명에서 제조된 누에분말을 유효성분으로 포함하는 혈당강하제는 종래의 열풍건조시킨 누에분말보다 혈당상승 억제효과가 우수하였으며, 그를 사용한 당뇨병 치료 방법은 식후 혈당치의 최고 정점인 식후 45 내지 60분 사이에서 기존 제제들의 불일치(mismatch) 현상을 거의 보완하고, 90분이후에도 혈당량의 완만한 하강곡선 현상을 나타냄으로써, 식후 고혈당과 공복시 저혈당을 막을 수 있는 가장 이상적인 혈당량 조절방법임이 확인되었다.