公开(公告)号：KR100824365B1

公开(公告)日：2008-04-22

申请号：KR1020060119768

申请日：2006-11-30

Applicant: 한국과학기술연구원

Inventor： 엄병헌 ,  정상훈 ,  김철영 ,  이희주 ,  이은하 ,  이정민

IPC: A61K36/899 ,  A61P3/10

Abstract: A composition comprising an extract of Phyllostachys nigra MUNRO, a fraction thereof or a compound isolated from the fraction is provided to inhibit accumulation of sorbitol, a fundamental cause of nephropathy and neuropathy shown as diabetic complications, by inhibiting aldose reductase, thereby being used as a pharmaceutical composition or a health food for preventing diseases related to the diabetic complications. A method for isolating a compound from Phyllostachys nigra MUNRO comprises the steps of: (a) extracting the Phyllostachys nigra MUNRO with ethanol and filtering the extract; (b) concentrating and freeze-drying the filtrate to obtain an extract; (c) fractionating an ethylacetate layer from the extract using a separation funnel; and (d) subjecting the fraction to silica gel column chromatography using CH2Cl2-MeOH as a solvent to obtain orientin, luteolin 6-C-(6''-O-transcaffeoylglucoside), tricetin 3',4',5'-trimethyl ether 7-O-b-D-glucopyranoside, tricine, p-coumaric acid or cis-coumaric acid. A pharmaceutical composition for preventing or treating diabetic retinopathy, diabetic neuropathy or diabetic nephropathy comprises the obtained compound as an effective ingredient.

Abstract translation: 提供了包含黑曲霉MUNRO提取物，其级分或从该级分分离的化合物的组合物，通过抑制醛糖还原酶来抑制山梨糖醇的积累，其是糖尿病并发症所示的肾病和神经病变的根本原因，因此被用作 药物组合物或用于预防与糖尿病并发症相关的疾病的保健食品。 一种从黑胫虫MUNRO中分离化合物的方法，包括以下步骤：（a）用乙醇萃取黑曲霉MUNRO，过滤提取物; （b）浓缩和冷冻滤液以获得提取物; （c）使用分液漏斗从提取物中分出乙酸乙酯层; 和（d）使用CH 2 Cl 2 -MeOH作为溶剂使级分进行硅胶柱色谱法，得到orientin，木犀草素6-C（6“-O-过氧基葡萄糖苷），三黄素3'，4'，5'-三甲基醚 7-ObD-吡喃葡萄糖苷，tricine，对香豆酸或顺式香豆酸。 用于预防或治疗糖尿病性视网膜病变，糖尿病性神经病变或糖尿病性肾病的药物组合物包含获得的化合物作为有效成分。