公开(公告)号：US07169353B1

公开(公告)日：2007-01-30

申请号：US09936077

申请日：2000-03-09

Applicant: Bruno Colin ,  Marie Privat

Inventor： Bruno Colin ,  Marie Privat

IPC: G01N33/00

CPC classification number: B01L3/502792 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/0406

Abstract: An apparatus (1) which includes at least one planar surface (2) having compartments (3) which are defined by a partition (4), the compartments creating a space which makes it possible to displace at least one liquid sample (5 and/or 15) and, when there are at least two liquid samples (5 and 15), makes it possible to displace them both in an independent way and bring them together so that they can react with one another. The compartments (3) include at least two different types of groove: a deep groove (6), capable of partitioning the sample(s) (5 and/or 15), and a shallow groove capable of receiving the sample (s) (5 and/or 15), the two types of groove (6 and 16) making it possible to direct sample movements (5 and/or 15) by altering the orientation of the apparatus (1). The invention is particularly applicable for the micromanipulation of fluids in biological applications.

Abstract translation: 一种装置（1），其包括至少一个具有由隔板（4）限定的隔室（3）的平坦表面（2），所述隔室产生空间，所述空间使得至少一个液体样品（5和/ 或15），并且当存在至少两个液体样品（5和15）时，可以以独立的方式将它们两者置于一起，使它们彼此反应。 隔室（3）包括至少两种不同类型的槽：能够分隔样品（5和/或15）的深槽（6）和能够接收样品的浅槽（ 5和/或15），两种类型的凹槽（6和16）使得可以通过改变设备（1）的取向来引导样品移动（5和/或15）。 本发明特别适用于生物应用中流体的显微操作。

公开(公告)号：US07159618B2

公开(公告)日：2007-01-09

申请号：US10559454

申请日：2004-06-15

Applicant: Patrick Broyer ,  Bruno Colin ,  Denis Roller

Inventor： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC: F15C1/04 ,  F15C1/06

CPC classification number: F16K99/0001 ,  F15C5/00 ,  F16K99/0036 ,  F16K99/0067 ,  F16K2099/0074 ,  F16K2099/008 ,  Y10T137/2082 ,  Y10T137/2196 ,  Y10T137/2224

Abstract: The invention relates to an electrically-opened micro fluid-valve. The inventive valve comprises: at least one planar support (12); at least one afferent microchannel (14); at least one efferent microchannel (16), said microchannels being arranged in the support (12); such that at least one of the ends (141 and 161) thereof touch the inner face (121) of the support close to one another; at least one deposit (18) of a heat-sensitive material which is disposed on the inner face (121) of the support (12); and at least one heating means (20). According to the invention, the aforementioned deposit (18) of heat-sensitive material at least partially seals the space separating the ends (141 and 161) of the microchannels (14 and 16), thereby preventing same from intercommunicating. When the temperature of the above-mentioned heating means (20) is raised, the structure of the heat-sensitive material is altered, thereby at least partially opening the ends of the microchannels an the space therebetween, in order to enable communication between said microchannels and to restore the flow of the fluid.

Abstract translation: 本发明涉及一种电开式微流体阀。 本发明的阀包括：至少一个平面支撑件（12）; 至少一个传入微通道（14）; 至少一个传出微通道（16），所述微通道布置在所述支撑件（12）中; 使得其端部（141和161）中的至少一个接触彼此的支撑件的内表面（121）; 设置在所述支撑件（12）的内表面（121）上的至少一个热敏材料沉积物（18）; 和至少一个加热装置（20）。 根据本发明，上述热敏材料沉积物（18）至少部分地密封分隔微通道（14和16）的端部（141和161）的空间，从而防止其相互通信。 当上述加热装置（20）的温度升高时，热​​敏材料的结构被改变，从而至少部分地将微通道的端部打开它们之间的空间，以便使所述微通道之间的连通 并恢复流体的流动。

公开(公告)号：US20060263871A1

公开(公告)日：2006-11-23

申请号：US11438938

申请日：2006-05-22

Applicant: Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Paris ,  Jacques Dachuad

Inventor： Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Paris ,  Jacques Dachuad

IPC: C12M1/36 ,  C12M1/34

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Abstract translation: 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US07134445B2

公开(公告)日：2006-11-14

申请号：US10531947

申请日：2003-11-18

Applicant: Patrick Broyer ,  Bruno Colin ,  Denis Roller

Inventor： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC: F16K7/12 ,  F15B15/19 ,  B81B3/00

CPC classification number: F16K99/0001 ,  F15C5/00 ,  F16K99/0015 ,  F16K99/0067 ,  F16K2099/008 ,  H01H1/0036 ,  H01H39/00 ,  Y10T137/1647

Abstract: The invention concerns the technical field of micro-actuators used for mechanical, chemical, electrical or thermal functions in microsystems, for microelectronic applications such as chips, or biomedical functions such as microfluidics integrating cards. The invention concerns a micro-actuator comprising a so-called main chamber, made in a solid support and containing a so-called main pyrotechnic charge, the main chamber being sealed and delimited by solid support walls and by a deformable membrane, such that the gases emitted by the combustion of the main pyrotechnic charge, enable the volume of the chamber to be increased by deforming the membrane while maintaining intact the solid walls of the main chamber. The invention is characterized in that it comprises means for evacuating the gases from the main chamber.

Abstract translation: 本发明涉及用于微系统中的机械，化学，电或热功能的微致动器的技术领域，用于微电子应用如芯片或生物医学功能，例如集成卡的微流体。 本发明涉及一种微型致动器，其包括所谓的主室，其形成为固体支撑并且包含所谓的主要烟火充电，所述主室由实心支撑壁和可变形膜密封并界定，使得 通过主烟火装置的燃烧发出的气体使得能够通过使膜变形而保持室的体积，同时保持主室的实心壁。 本发明的特征在于其包括用于从主室排出气体的装置。

公开(公告)号：US20060096486A1

公开(公告)日：2006-05-11

申请号：US10552583

申请日：2004-04-09

Applicant: Denis Roller ,  Patrick Broyer ,  Bruno Colin

Inventor： Denis Roller ,  Patrick Broyer ,  Bruno Colin

IPC: F42C19/08

CPC classification number: F42B3/10 ,  F41A19/65 ,  F42B3/006 ,  F42C19/12

Abstract: The invention relates to a device for the electrical initiation of at least one pyrotechnic microcharge (3), this device being characterized in that it comprises a support element having at least one conductive finger (6) connected to a first terminal of a central control unit (8), a second terminal of said central control unit (8) being intended to be electrically connected to an electrically conductive support for the pyrotechnic microcharge (3). The invention also relates to a microactuator (1, 1a, . . . , 1h) and a microsystem (1′) that use such an initiation device.

Abstract translation: 本发明涉及一种用于电启动至少一个烟火微电池（3）的装置，该装置的特征在于其包括具有至少一个导电指状物（6）的支撑元件，该导电指状物（6）连接到中央控制单元 （8），所述中央控制单元（8）的第二端子旨在电连接到用于烟火微电池（3）的导电支撑件。 本发明还涉及使用这种引发装置的微致动器（1,1a，...，1h）和微系统（1'）。

公开(公告)号：US20050244887A1

公开(公告)日：2005-11-03

申请号：US11180806

申请日：2005-07-12

Applicant: Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Abstract translation: 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US06949376B2

公开(公告)日：2005-09-27

申请号：US10141049

申请日：2002-05-07

Applicant: Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00 ,  C12M1/36

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

公开(公告)号：US06854376B2

公开(公告)日：2005-02-15

申请号：US10258021

申请日：2001-04-23

Applicant: Bruno Colin

Inventor： Bruno Colin

IPC: A61B5/15 ,  B01J4/00 ,  B01L3/02 ,  F16J1/10 ,  F01B25/26

CPC classification number: F16J1/10 ,  B01J4/00 ,  B01L3/021

Abstract: A device (1) for actuating at least one piston (2) moving axially in the bore (3) of a body (4), the piston (2) being driven by the longitudinal or curved movement (F2) of a control arm (5). Also disclosed is a method for detecting a failure in a device (1) having an intermediary element (6), located between the piston (2) and the control arm (5), this intermediary element (6) being also used as an alarm means should the intermediary element (6) be missing between the piston (2) and the arm (5). The invention is particularly applicable in the field of diagnosis.

Abstract translation: 一种用于致动在主体（4）的孔（3）中轴向移动的至少一个活塞（2）的装置（1），活塞（2）由控制臂的纵向或弯曲运动（F2）驱动 5）。 还公开了一种用于检测具有位于活塞（2）和控制臂（5）之间的中间元件（6）的装置（1）中的故障的方法，该中间元件（6）也用作报警器 中间元件（6）在活塞（2）和臂（5）之间缺少的装置。 本发明特别适用于诊断领域。

公开(公告)号：US06632662B1

公开(公告)日：2003-10-14

申请号：US09743723

申请日：2001-03-02

Applicant: Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

Inventor： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

IPC: C12M133

CPC classification number: C12N1/066 ,  C12M47/06

Abstract: The invention concerns a device for lysis (1) of micro-organism to release at least one intracellular biological constituent, comprising a container (2) wherein are present a biological sample, in liquid medium, containing the micro-organism to be lyzed, and a material in the form of particles, relatively hard and inert with respect to the sample. The invention also concerns a grinding method. The material in the form of particles comprises at least two types of grinding means into different dimensions: at least large dimension magnetic means (3) automatically controlled by a magnetic field; and at least small dimension means (4) actuated by the large dimension grinding means (3). The invention is useful for separating biological constituents.

Abstract translation: 本发明涉及用于释放至少一种细胞内生物成分的微生物的裂解装置（ 1 ），其包含容器（ 2 ），其中存在生物样品，在含有要溶解的微生物的液体培养基中，以及相对于样品相对较硬且惰性的颗粒形式的材料。 本发明还涉及研磨方法。 颗粒形式的材料包括至少两种不同尺寸的研磨装置：由磁场自动控制的至少大尺寸磁性装置（ 3 ）; 以及由大尺寸研磨装置（ 3 ）致动的至少小尺寸装置（ 4 ）。 本发明可用于分离生物成分

公开(公告)号：US06458553B1

公开(公告)日：2002-10-01

申请号：US09763757

申请日：2001-05-14

Applicant: Bruno Colin ,  Cecile Paris ,  Thierry Sofia ,  Claude Terrot

Inventor： Bruno Colin ,  Cecile Paris ,  Thierry Sofia ,  Claude Terrot

IPC: C12Q102

CPC classification number: C12M41/36 ,  B01L3/502 ,  B01L3/5027 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0457

Abstract: The invention concerns an analysis card as well as a method for filing such a card and the use of said card. Said card comprises a body consisting of: a zone for injecting into the card a biological fluid to be analyzed; a main duct supplying the biological fluid coming from the injection zone; at least two secondary ducts located in the extension of the main duct; and at least one well corresponding to each secondary duct. The invention is characterized in that each well constitutes a means for dispensing a specific volume of said biological fluid into at least two terminal analysis ducts, via a terminal duct for each analysis cavity and the volume to be analyzed is less than the total volume of all the analysis ducts. Although this concerns a particular embodiment wherein a biological fluid is transferred in parallel, in another embodiment the transfer may be carried out in series. The invention is particularly applicable in microbiology.

Abstract translation: 本发明涉及一种分析卡以及用于提交这种卡和使用所述卡的方法。 所述卡片包括：身体，其包括：用于向所述卡片注入待分析的生物流体的区域; 供应来自注射区域的生物流体的主管道; 至少两个辅助管道位于主管道的延伸部分中; 以及对应于每个次级管道的至少一个孔。 本发明的特征在于，每个井构成用于将特定体积的所述生物流体分配到至少两个终端分析管道中的装置，通过每个分析腔的终端管道，并且待分析的体积小于全部体积 分析管道。 尽管这涉及其中生物流体被并行转移的特定实施方案，但是在另一个实施方案中，转移可以串联进行。 本发明特别适用于微生物学。