公开(公告)号：US07214529B2

公开(公告)日：2007-05-08

申请号：US11180806

申请日：2005-07-12

Applicant: Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12M1/36

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Abstract translation: 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US20050244887A1

公开(公告)日：2005-11-03

申请号：US11180806

申请日：2005-07-12

Applicant: Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Abstract translation: 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US06949376B2

公开(公告)日：2005-09-27

申请号：US10141049

申请日：2002-05-07

Applicant: Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00 ,  C12M1/36

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

公开(公告)号：US06429007B1

公开(公告)日：2002-08-06

申请号：US09420140

申请日：1999-10-18

Applicant: Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

Inventor： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC: C12M136

CPC classification number: C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Abstract translation: 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US5989499A

公开(公告)日：1999-11-23

申请号：US053823

申请日：1998-04-02

Applicant: Luigi Catanzariti ,  Bryan W. Kluttz ,  Bruno Colin ,  Cecile Jaravel

Inventor： Luigi Catanzariti ,  Bryan W. Kluttz ,  Bruno Colin ,  Cecile Jaravel

IPC: C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N37/00

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2400/049 ,  B01L2400/0644 ,  B01L2400/0655

Abstract: A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction.A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an autlomated fashion without human intervention.

Abstract translation: 用于核酸扩增反应的反应容器具有包含扩增试剂混合物的第一室，含有扩增酶的第二室和将第一和第二室连接在一起的流体通道或室。 将流体样品引入第一室。 在第一室中发生变性和引物退火过程之后，打开流体通道以允许试剂和流体样品的溶液流入第二室。 第二个室保持在扩增反应的最佳温度。 描述了一个工位，用于处理加入反应容器的试片。 该站包括温度和真空控制子系统，以在反应容器中保持适当的温度，并且以无人的干预的方式实现流体从一个室到另一个室的转移。

公开(公告)号：US6015531A

公开(公告)日：2000-01-18

申请号：US983492

申请日：1998-03-05

Applicant: Bruno Colin ,  Cecile Jaravel ,  Philippe Cleuziat

Inventor： Bruno Colin ,  Cecile Jaravel ,  Philippe Cleuziat

IPC: G01N33/543 ,  B01L3/00 ,  G01N35/02 ,  G01N21/00

CPC classification number: B01L3/502

Abstract: The invention features an analysing device (1) comprising a body (2) in which are arranged or provided: an intake aperture (3) for a starting liquid sample, a liquid flow circuit (5) comprising at least one operating cell (6) for a processed liquid sample, obtained from all or part of the original sample, communicating with the said intake aperture (3), the said flow circuit defining, in at least two dimensions of the card, one determined geometric line, such that any alteration in the card orientation in a three-dimensional reference frame, causes the liquid to flow under gravity only, from one part of the said circuit to another,for instance from one side or another of the operating cell, (6) characterised in that, the flow circuit (5) is continuous, and looped on itself between the said aperture (3) and the said operating cell (6).

Abstract translation: PCT No.PCT / FR97 / 01020 Sec。 371日期：1998年3月5日 102（e）1998年3月5日PCT 1997年6月9日PCT公布。 WO97 / 46318 PCT公开号 日期1997年12月11日本发明的特征在于一种分析装置（1），包括其中布置或提供的主体（2）;用于起始液体样品的进气孔（3），包括至少一个 从所述原始样品的全部或部分获得的用于经处理的液体样品的操作室（6）与所述进气孔（3）连通，所述流动回路在卡的至少两个维度上限定一个确定的几何线 ，使得三维参考系中的卡方向的任何改变使得液体仅在重力作用下从所述电路的一部分流向另一部分，例如来自操作单元的一侧或另一侧（6 ），其特征在于，流动回路（5）是连续的，并且在所述孔（3）和所述操作单元（6）之间自身环绕。