公开(公告)号：US20050099622A1

公开(公告)日：2005-05-12

申请号：US11019439

申请日：2004-12-21

Applicant: Stephen Caracci ,  Norman Fontaine ,  Eric Mozdy ,  Po Yuen

Inventor： Stephen Caracci ,  Norman Fontaine ,  Eric Mozdy ,  Po Yuen

IPC: G01N21/25 ,  G02B27/10 ,  G01J3/50

CPC classification number: G01N21/253 ,  G01N2201/04 ,  G01N2201/10 ,  G02B27/1086

Abstract: Optical interrogation systems and methods are described herein that are capable of measuring the angles (or changes in the angles) at which light reflects, transmits, scatters, or is emitted from an array of sensors or specimens that are distributed over a large area 2-dimensional array. In one embodiment of the present invention, the optical interrogation system has a far-field diffraction measurement configuration so it can simultaneously receive the light from sensors in all or a sub-section of a sensor array at a detector. In another embodiment of the present invention, the optical interrogation system incorporates an anamorphic optical receive system that enables parallel detection of angular responses from the sensors in the 2D array. In yet another embodiment of the present invention, the optical interrogation system incorporates an angular measurement system which measures the change in angular tilt of the sensor plane when the sensor array is moved or removed and then replaced in the measurement system. Several other embodiments of optical interrogation systems and methods are also described herein. A significant advantage of the present invention is that the system has no critical moving or scanning parts, which are frequently the source of measurement sensitivity limitations.

Abstract translation: 本文描述了光学询问系统和方法，其能够测量光从其分布在大面积上的传感器或样本阵列2反射，透射，散射或发射的角度（或角度变化） 维数组。 在本发明的一个实施例中，光学询问系统具有远场衍射测量配置，因此它可以在检测器处同时从传感器阵列的全部或子部分接收来自传感器的光。 在本发明的另一实施例中，光学询问系统包括变形光学接收系统，其能够并行地检测来自2D阵列中的传感器的角度响应。 在本发明的另一个实施例中，光学询问系统包括角度测量系统，该系统测量当传感器阵列移动或移除并随后在测量系统中被更换时传感器平面的角度倾斜度的变化。 本文还描述了光询问系统和方法的其他几个实施例。 本发明的显着优点是该系统没有关键的移动或扫描部件，其经常是测量灵敏度限制的来源。

公开(公告)号：US5369007A

公开(公告)日：1994-11-29

申请号：US981857

申请日：1992-08-26

Applicant: David A. Kidwell

Inventor： David A. Kidwell

IPC: G01N33/53 ,  B01L3/00 ,  G01N21/03 ,  G01N21/29 ,  G01N21/76 ,  G01N21/78 ,  G01N31/20 ,  G01N33/52 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552 ,  G01N33/94

CPC classification number: G01N33/54386 ,  B01L3/5023 ,  G01N21/78 ,  G01N33/525 ,  B01L2200/0621 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406 ,  G01N21/29 ,  G01N21/76 ,  G01N2201/04 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/81

Abstract: A microassay card includes an upper layer containing wells for receiving a liquid sample. A second layer of the card, beneath the first layer, includes a supporting surface bound to a reactive species. A third layer includes a superabsorbent support impregnated with an indicator. Typically, the indicator is a substrate for an enzyme, such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme upon the substrate to cause a spectral change in the absorbent layer. By selecting the structure of the first and second layers, the card can be formatted for a displacement assay or a competitive assay. The microassay card of the present invention is particularly useful for drug testing.

Abstract translation: 微型卡片包括含有用于接收液体样品的孔的上层。 在第一层下方的卡的第二层包括与活性物质结合的支撑表面。 第三层包括浸渍有指示剂的超吸收剂载体。 通常，指示剂是用于酶的底物，例如还原的染料前体和过氧化氢源，用于酶在底物上起作用以引起吸收层中的光谱变化。 通过选择第一层和第二层的结构，可以将卡格式化为位移测定或竞争性测定。 本发明的微量测定卡对于药物测试特别有用。

公开(公告)号：US5184193A

公开(公告)日：1993-02-02

申请号：US293218

申请日：1989-01-04

Applicant: David A. LeFebre

Inventor： David A. LeFebre

IPC: G01J1/18 ,  G01J3/06 ,  G01J3/08 ,  G01J3/42

CPC classification number: G01J3/08 ,  G01J2001/182 ,  G01J2003/068 ,  G01J3/42 ,  G01N2201/04 ,  G01N2201/0833

Abstract: Spectrophotometric apparatus and methodology suitable for continuous and long-term use. The apparatus includes a monochromator providing pre-dispersed monochromatic light to the optical inputs of a pair of fiber optic cables and a translator for alternatively positioning the fiber optic cables at the same location with respect to the monochromator output. One of the cables conducts light to a sample under study while the other cable provides a reference for light intensity measurements. The methodology includes the steps of performing two scans through the monochromator output for each measurement on the sample. The fiber optic cables are moved between scans so that the cable employed in the latter scan occupies the former position of the cable employed in prior scan.

Abstract translation: 分光光度仪和方法适用于连续和长期使用。 该装置包括单色仪，其将预分散的单色光提供给一对光纤电缆的光输入端，以及用于相对于单色仪输出将光纤缆线替代地定位在相同位置处的转换器。 其中一根电缆对正在研究的样品进行光照，而另一根电缆为光强测量提供参考。 该方法包括通过单色仪输出进行两次扫描以对样本进行每次测量的步骤。 光纤电缆在扫描之间移动，使得在后一扫描中使用的电缆占据先前扫描中使用的电缆的前一位置。

公开(公告)号：US4826660A

公开(公告)日：1989-05-02

申请号：US046953

申请日：1987-05-07

Applicant: Roger E. Smith ,  Randall W. Smith

Inventor： Roger E. Smith ,  Randall W. Smith

IPC: G01N21/64 ,  G01N21/25 ,  G01N21/27 ,  G01N35/02

CPC classification number: G01N21/255 ,  G01N2201/04

Abstract: A detector assembly for use with an analyzer instrument comprises a light source for providing a light beam for analyzing one or more substances in a sample. A detector is included for detecting light associated with the sample under analysis. An analysis station permits the light beam to enter the sample and shields the sample from extraneous light. The analysis station further permits light to pass from the sample to the light detector. A plurality of filters is selectively positionable in the light beam between the light source and the sample. These filters permit different wavelengths of light to pass therethrough so that selective light analysis of the sample may be performed.

公开(公告)号：US4668636A

公开(公告)日：1987-05-26

申请号：US793345

申请日：1985-10-31

Applicant: Anthony Ringrose ,  Ranald M. Sutherland ,  Claus Dahne ,  John F. Place

Inventor： Anthony Ringrose ,  Ranald M. Sutherland ,  Claus Dahne ,  John F. Place

IPC: G01N21/03 ,  G01N21/77 ,  G01N21/75 ,  G01N33/552

CPC classification number: G01N21/7703 ,  G01N21/03 ,  G01N2021/0325 ,  G01N2201/04 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/81

Abstract: An apparatus responsive to optical changes in a waveguide when the latter is contacted with an analyte capable of reacting with a specific reactant thereto attached to the surface of said guide involves, for containing the analyte, an interchangeable round bottomed cuvette temporarily held by a suitably shaped carrier in working relationship with the optical components of the apparatus, this arrangement ensuring a correct optical orientation thereof relative to said component whatever the exact positioning of the cuvette.

Abstract translation: 当波导中的光学变化与能够与附着在所述导向件的表面上的特定反应物反应的分析物接触时，波导中的光学变化的装置包括用于容纳分析物的可互换的圆底比色皿，其被暂时保持在适当形状 载体与设备的光学部件处于工作关系，这种布置确保了相对于所述部件的正确的光学取向，无论比色杯的确切位置如何。

公开(公告)号：US4575492A

公开(公告)日：1986-03-11

申请号：US602509

申请日：1984-04-20

Applicant: Pierre David ,  Jean A. Jarricot

Inventor： Pierre David ,  Jean A. Jarricot

IPC: G01N21/17 ,  G01N21/59 ,  G01N21/82 ,  G01N33/49 ,  G01N33/80 ,  G01N33/86 ,  G01N33/53

CPC classification number: G01N21/82 ,  G01N2021/5934 ,  G01N2201/04 ,  Y10S436/805

Abstract: The present invention relates to a process and device for the detection and quantification of agglutinates, in recipients of which at least the bottom are transparent. According to the invention, it is decided that there is agglutination when, at the same time, the calculated mean value of the bottom of the reaction studied is higher than that of a reference sample and when there exist points of measurement whose transmission is less than said threshold, and, on the other hand, that there is no agglutination when, at the same time, this calculated mean value is not greater than that of said reference sample and when there exist no points of measurement whose transmission is less than said threshold. The invention is more particularly applicable to immunohaematology.

Abstract translation: 本发明涉及用于检测和定量凝集物的方法和装置，其中至少底部是透明的受体。 根据本发明，当所研究的反应的底部的计算平均值高于参考样品的平均值时，当存在透射小于测量点的测量点时，确定存在凝集 所述阈值，另一方面，当同时计算的平均值不大于所述参考样本的平均值时，不存在凝集，并且当不存在透射小于所述阈值的测量点时 。 本发明更特别适用于免疫血液学。

公开(公告)号：US4536655A

公开(公告)日：1985-08-20

申请号：US527366

申请日：1983-08-29

Applicant: Clarence W. Barnes

Inventor： Clarence W. Barnes

IPC: G01N21/64 ,  G01L1/20

CPC classification number: G01N21/6452 ,  G01N2201/04

Abstract: A fluorometer system is disclosed in which a carousel-like system is rotated by a stepper motor to provide a sequence of sample wells to a single optical exciting path. An ultraviolet tube positioned adjacent that path provides an excitation beam which is reflected into common optical path which coincides with the position of the well along the common path is deflected by a beam splitter or the like through an appropriate filtering device to a photomultiplier tube which is located adjacent the common light path on the same side of the path as the ultraviolet tube. The photomultiplier tube is located below the ultraviolet tube to provide a proper direction of convection current.Further, a solid state photosensor sensitive in red spectrum such as a phototransistor or photodiode is provided adjacent the ultraviolet tube, with a ruby positioned between the ultraviolet tube and the photosensor the take advantage of the fact that solid-state photosensors are especially responsive to light in the red range. The output of the photosensor is fed back to the A to D converter and microprocessor software to take account for the fact that with tube aging or fluctuation in the line current, the output of the ultraviolet tube may vary.

Abstract translation: 公开了一种荧光计系统，其中转盘式系统由步进电机旋转以向单个光学激发路径提供一系列样品阱。 位于该路径附近的紫外线管提供激发光束，其被反射到共同的光路中，这与沿着公共路径的井的位置重合，由分束器等通过适当的过滤装置偏转到光电倍增管，光电倍增管是 位于与紫外线管相同的一侧的公共光路附近。 光电倍增管位于紫外线管下方，以提供适当的对流方向。 此外，在红外光谱下敏感的固体光敏传感器如光电晶体管或光电二极管被设置在紫外线管附近，红宝石位于紫外线管和光电传感器之间，利用固态光电传感器特别适用于光 在红色范围内。 将光电传感器的输出反馈到A到D转换器和微处理器软件，以考虑到管线老化或线路电流波动的事实，紫外线管的输出可能变化。

公开(公告)号：WO2016127090A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016832

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MARKS, Jeffrey

IPC: G01N21/27 ,  G01N21/64

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. The method (800) comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument (805, 820) for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors (840) for each filter pair to result in corrected pure dye spectra, then normalized (845) and the multicomponenting data can be extracted (850).

Abstract translation: 本教导涉及用于在多个仪器上归一化光谱的方法和系统。 方法（800）包括至少一个参考仪器和测试仪器。 每个仪器包括至少一个激励滤波器和成对布置的至少一个发射滤波器。 每个仪器还包括包含多个孔的纯染料板。 每个孔含有多种染料，其中每种染料包含荧光组分。 对于每种染料，通过多个过滤器组合从每个仪器（805,820）获得荧光光谱，以有助于参考仪器的纯染料矩阵Mref和测试仪器的纯染料矩阵M. 然后可以将纯染料光谱乘以每个过滤器对的校正因子（840），以产生校正的纯染料光谱，然后归一化（845），并且可以提取多组分数据（850）。

公开(公告)号：WO2013091146A1

公开(公告)日：2013-06-27

申请号：PCT/CN2011/002162

申请日：2011-12-22

Applicant: HONEYWELL INTERNATIONAL INC. ,  SHEN, Yanming ,  XU, Hongyan ,  LI, Haohua

Inventor： SHEN, Yanming ,  XU, Hongyan ,  LI, Haohua

IPC: G01N21/59 ,  G01N21/01

CPC classification number: G01N21/59 ,  G01N21/01 ,  G01N33/442 ,  G01N2201/04

Abstract: Polymeric fibers may be tested for optical properties; such as transparency in a manner that provides reproducible test results. A holder be used that is configured to hold polymeric fibers in a stacked, single file manner in which the polymeric fibers are held closely together. The fibers may be tested using an optical apparatus employing an integrating sphere.

Abstract translation: 可以测试聚合纤维的光学性能; 例如以提供可重现的测试结果的方式的透明度。 使用的保持器被构造成以聚合物纤维紧密地保持在一起的堆叠的单一文件方式保持聚合物纤维。 纤维可以使用采用积分球的光学装置进行测试。

公开(公告)号：WO2006113177A3

公开(公告)日：2009-04-09

申请号：PCT/US2006013080

申请日：2006-04-06

Applicant: UNIV WASHINGTON ,  CHINOWSKY TIMOTHY M ,  SOELBERG SCOTT D ,  KAUFFMAN PETER C ,  FURLONG CLEMENT E ,  TRITZ JARED ,  GROW MICHAEL S ,  NAIMUSHIN ALEXEI N ,  YEE SINCLAIR S

Inventor： CHINOWSKY TIMOTHY M ,  SOELBERG SCOTT D ,  KAUFFMAN PETER C ,  FURLONG CLEMENT E ,  TRITZ JARED ,  GROW MICHAEL S ,  NAIMUSHIN ALEXEI N ,  YEE SINCLAIR S

IPC: G01N21/55

CPC classification number: G01N21/553 ,  G01N2201/04

Abstract: Various improvements in the field of SPR sensing systems are disclosed. One improvement relates to a portable SPR sensing system within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system, in which selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/ disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system, by providing for the elimination of false positives and increased sensitivity by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. Protocols are provided for the detection of small molecules and employ a competition type assay where the presence of the analyte inhibits the binding of antibodies to surface immobilized analyte, or a displacement assay, where antibodies bound to the analyte on the sensor surface are displaced by free analyte.

Abstract translation: 公开了SPR传感系统领域的各种改进。 一个改进涉及可以手提到监控地点的手提箱内的便携式SPR感测系统。 另一个改进涉及一种便携式基于盒式SPR感测系统，其中系统的电和流体系统的选定部分被分配在基本单元和可移动/一次性盒之间。 其他改进涉及用于操作SPR感测系统的方法或协议，通过使用针对不同靶表位的特异性的二次抗体和传感器元件冗余来提供消除假阳性并增加灵敏度。 提供了用于检测小分子的方案，并采用竞争型测定法，其中分析物的存在抑制抗体与表面固定化分析物的结合，或位移测定法，其中与传感器表面上的分析物结合的抗体被免费位移 分析物。