公开(公告)号：US20120156100A1

公开(公告)日：2012-06-21

申请号：US12972546

申请日：2010-12-20

Applicant: Rung-Ywan Tsai ,  Cheng-Wei Chu ,  Hsiu-Hsiang Chen

Inventor： Rung-Ywan Tsai ,  Cheng-Wei Chu ,  Hsiu-Hsiang Chen

IPC: G01N21/64 ,  H01L21/77 ,  B82Y99/00 ,  B82Y40/00

CPC classification number: G01N21/6454 ,  B82Y40/00 ,  G01N21/6428 ,  G01N21/648 ,  G01N2201/0628

Abstract: The present invention directs to a detection apparatus for detecting the fluorescence signal emitting from a single and individual analyte molecule. By integrating the excitation light source, the detector array and the nanowell array all together within the detection apparatus, the single analyte molecule trapped in the nanowell can be excited by the light source and emits fluorescence signal to the detector array.

Abstract translation: 本发明涉及用于检测从单个和单个分析物分子发射的荧光信号的检测装置。 通过将激发光源，检测器阵列和纳米线阵列整合在检测装置内，捕获在纳米线中的单个分析物分子可被光源激发并向检测器阵列发射荧光信号。

公开(公告)号：US20120087832A1

公开(公告)日：2012-04-12

申请号：US13333788

申请日：2011-12-21

Applicant: Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

Inventor： Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

IPC: G01N21/64 ,  G01N21/63

CPC classification number: G01N21/6428 ,  B01L7/52 ,  B01L2300/1805 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0628 ,  Y10S435/808

Abstract: An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.

Abstract translation: 提供一种用于通过激发波长区分多个可检测信号的装置和方法。 该装置可以包括可以例如在一个井中的样品保留区域中的样品发射相应的激发光波长或波长范围的光源。 样品可以包含两种或更多种可检测的标记，例如荧光染料，其中当荧光染料在靶组分存在下被激发时，其能够产生增加的可检测的发射。 可检测标记物可以具有与其它可检测标记物的范围重叠的激发波长范围和/或发射波长范围。 可以设置检测器，用于检测在至少一个其他标记的重叠波长范围内从第一标记发射的发射波长或波长范围。

公开(公告)号：US20120056205A1

公开(公告)日：2012-03-08

申请号：US13148040

申请日：2010-02-03

Applicant: Bart A.M. Allard ,  Ruben Bernardus Alfred Sharpe

Inventor： Bart A.M. Allard ,  Ruben Bernardus Alfred Sharpe

IPC: H01L31/12

CPC classification number: G01N21/253 ,  G01N21/6445 ,  G01N21/6454 ,  G01N21/7703 ,  G01N2021/7723 ,  G01N2021/7783 ,  G01N2201/0628 ,  H01L25/046 ,  H01L27/288 ,  H01L2924/0002 ,  H01L2924/00

Abstract: In one aspect, the invention provides an optical sensor comprising a flexible substrate and an optical element being positioned on the substrate. The flexible substrate comprises deformations affecting the optical element and the deformations are provided in a substrate deformation zone at least partly surrounding the optical element.It is an object of the present invention to provide an optical sensor configuration compatible with roll-to-roll manufacturing.

Abstract translation: 一方面，本发明提供了一种光学传感器，其包括柔性基板和位于基板上的光学元件。 柔性基板包括影响光学元件的变形，并且变形被设置在至少部分地围绕光学元件的基板变形区域中。 本发明的目的是提供一种与卷对卷制造兼容的光学传感器构造。

公开(公告)号：US07718130B1

公开(公告)日：2010-05-18

申请号：US11401274

申请日：2006-04-11

Applicant: Ruth Shinar ,  Joseph Shinar ,  Vikram L. Dalal

Inventor： Ruth Shinar ,  Joseph Shinar ,  Vikram L. Dalal

IPC: G01N21/64 ,  G01N21/00 ,  F21V9/16 ,  G01N21/76 ,  G02B6/00

CPC classification number: G01N21/6454 ,  B01L3/5027 ,  G01N2201/0628

Abstract: Integrated photoluminescence (PL)-based chemical and biological sensors are provided comprising a photodetector (PD), a long-pass filter, an excitation source, and a sensing element, all based on thin films or structures. In one embodiment the light source is an organic light emitting device (OLED) and the sensing element is based on thin films or solutions in microfluidic channels or wells. The PD and optical filters are based on thin film amorphous or nanocrystalline silicon and related materials. In another embodiment, sensor components are fabricated on transparent substrates, which are attached back-to-back to generate a compact, integrated structure.

Abstract translation: 提供基于集成光致发光（PL）的化学和生物传感器，其包括基于薄膜或结构的光电检测器（PD），长通滤波器，激发源和感测元件。 在一个实施例中，光源是有机发光器件（OLED），并且感测元件基于微流体通道或孔中的薄膜或溶液。 PD和滤光器基于薄膜非晶或纳米晶硅及相关材料。 在另一个实施例中，传感器部件制造在透明基板上，背板背对背连接以产生紧凑的集成结构。

公开(公告)号：US07498164B2

公开(公告)日：2009-03-03

申请号：US10456196

申请日：2003-06-06

Applicant: Mark F. Oldham ,  Eugene F. Young

Inventor： Mark F. Oldham ,  Eugene F. Young

IPC: C12M1/34 ,  C12M3/00 ,  G01N21/64 ,  G01J1/58 ,  F21V9/16

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An instrument is provided that can monitor nucleic acid sequence amplifications reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

Abstract translation: 提供了可以监测核酸序列扩增反应的仪器，例如DNA和DNA片段的PCR扩增。 仪器包括沿激发光束路径和发射光束路径中的一个或两个设置的多陷波滤波器。 还提供了使用包括沿着光束路径设置的多陷波滤波器的仪器来监测核酸序列扩增的方法。

公开(公告)号：US20090009767A1

公开(公告)日：2009-01-08

申请号：US12217410

申请日：2008-07-03

Applicant: Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

Inventor： Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

Abstract translation: 提供了一种光学仪器，用于同时用由光源产生的激发光束照射两个或更多个间隔开的反应区域。 光源可以包括发光二极管的区域光阵列，一个或多个固态激光器，一个或多个微线激光器，或其组合。 根据各种实施例，菲涅耳透镜可以沿着光源和反应区域之间的光束槽设置。 还提供了使用光学仪器的分析方法。

公开(公告)号：US20060199259A1

公开(公告)日：2006-09-07

申请号：US11406148

申请日：2006-04-18

Applicant: Anthony Cerrone ,  Edward Lakatos ,  Michael Gambini ,  Eugene Young ,  Susan Stone ,  Judith Atwood ,  John Atwood

Inventor： Anthony Cerrone ,  Edward Lakatos ,  Michael Gambini ,  Eugene Young ,  Susan Stone ,  Judith Atwood ,  John Atwood

IPC: C12M1/34

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An instrument for monitoring replication of DNA is provided.

Abstract translation: 提供了一种用于监测DNA复制的工具。

公开(公告)号：US07008789B2

公开(公告)日：2006-03-07

申请号：US10216620

申请日：2002-08-09

Applicant: Michael R. Gambini ,  Susan Atwood Stone, legal representative ,  Judith K. Atwood, legal representative ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

Inventor： John G. Atwood, deceased

IPC: C12M1/34

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US20030035755A1

公开(公告)日：2003-02-20

申请号：US09931447

申请日：2001-08-16

Inventor： Shu-Hui Chen ,  Chung-Shi Yang ,  Si-Chung Chang ,  Chun-Che Lin ,  Shih-Yao Sun

IPC: G01N021/76

CPC classification number: G01N21/645 ,  B01J2219/00659 ,  B01J2219/00702 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0628

Abstract: Organic electroluminescent (OEL) devices are proposed herein to be fabricated either as a light source or a heating source for biochips. Under the proposed approach, an OEL-emitting member is fabricated as the substrate of the biochip on which the biological samples, such as DNA, proteins and other related small molecules, are processed for the desired applications, including but not limited to, analysis of biological molecules, such as electrophoretic separation, PCR amplification and hybridization.

Abstract translation: 本文提出有机电致发光（OEL）器件作为生物芯片的光源或加热源制造。 在所提出的方法下，制造OEL发射构件作为生物芯片的底物，生物样品（例如DNA，蛋白质和其它相关小分子）在其上处理所需应用，包括但不限于 生物分子，如电泳分离，PCR扩增和杂交。

公开(公告)号：US20020192808A1

公开(公告)日：2002-12-19

申请号：US10216620

申请日：2002-08-09

Inventor： Michael R. Gambini ,  Susan Atwood Stone ,  Judith K. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

IPC: C12M001/34

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

Abstract translation: 光学仪器监测具有温度循环阻滞的反应装置中DNA的PCR复制，所述反应装置具有小瓶的反应成分，包括在双链DNA存在下发荧光的染料。 分束器将激发光束传递到小瓶以使染料发荧光。 来自染料的发射光束通过分束器传递到CCD检测器，处理器从该CCD检测器计算DNA浓度。 具有多个参考发射器的参考条发射不同强度的参考光束，处理器从该参考光束选择用于补偿漂移的最佳发射器。 自动调整曝光时间以保持CCD和处理器的最佳动态范围。 分束器和相关的滤光器的模块与选定的染料相关联，并且可替代不同的染料。