公开(公告)号：WO2011153500A2

公开(公告)日：2011-12-08

申请号：PCT/US2011/039173

申请日：2011-06-03

Applicant: APERIO TECHNOLOGIES, INC. ,  EICHHORN, Ole ,  PERZ, Cindy

Inventor： EICHHORN, Ole ,  PERZ, Cindy

IPC: G06T1/00 ,  G01N33/483 ,  G01N21/00 ,  G06T7/00 ,  H04N7/26

CPC classification number: G06K9/00127 ,  G06T7/0004 ,  G06T2207/10056 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30168 ,  H04N19/60 ,  H04N19/86

Abstract: A system that determines the quality of a digital microscope slide by analyzing digital slide images based on complexity and spatial frequencies. An example embodiment detailed in the application may provide visual feedback on the whole slide quality by overlaying the image with a color coded "heat map" of local area quality. A user provided with the overlap image may obtain both an absolute quality measurement for the whole image and quickly identity the quality variability within the slide.

Abstract translation: 通过基于复杂性和空间频率分析数字幻灯片图像来确定数字显微镜幻灯片的质量的系统。 应用中详细描述的示例实施例可以通过用局部区域质量的颜色编码的“热图”覆盖图像来提供关于整个幻灯片质量的视觉反馈。 提供重叠图像的用户可以获得整个图像的绝对质量测量，并且快速识别幻灯片内的质量变异性。

公开(公告)号：WO2011072211A3

公开(公告)日：2011-10-27

申请号：PCT/US2010059861

申请日：2010-12-10

Applicant: APERIO TECHNOLOGIES INC ,  OLSON ALLEN ,  EICHHORN OLE

Inventor： OLSON ALLEN ,  EICHHORN OLE

IPC: G06Q50/00 ,  G06T7/00

CPC classification number: G06T7/0012 ,  G06F19/321 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A digital slide analysis system comprises an algorithm server that maintains or has access to a plurality of image processing and analysis routines. The algorithm server additionally has access to a plurality of digital slide images. The algorithm server executes a selected routine on an identified digital slide and provides the resulting data. Prior to the application of selected routine, the system employs a digital pre-processing module to create a metadata mask that reduces undesirable image data such that the image data processed by the selected routine has an improved signal to noise ratio. The pre-processing module uses a classifier that may be implemented as a pattern recognition module, for example. Undesirable image data is therefore excluded from the image data that is processed by the digital pathology image processing and analysis routine, which significantly improves the digital pathology image analysis.

Abstract translation: 数字幻灯片分析系统包括算法服务器，该算法服务器维护或访问多个图像处理和分析例程。 算法服务器还可以访问多个数字载玻片图像。 算法服务器在识别的数字幻灯片上执行选定的例程并提供结果数据。 在应用所选例程之前，系统采用数字预处理模块来创建元数据掩码，该元数据掩码减少不需要的图像数据，使得由所选例程处理的图像数据具有改善的信噪比。 例如，预处理模块使用可以被实现为模式识别模块的分类器。 因此不合意的图像数据被从数字病理图像处理和分析程序处理的图像数据中排除，这显着改善了数字病理图像分析。

公开(公告)号：WO2006081362A3

公开(公告)日：2006-08-03

申请号：PCT/US2006/002799

申请日：2006-01-27

Applicant: APERIO TECHNOLOGIES, INC ,  EICHHORN, Ole

Inventor： EICHHORN, Ole

IPC: G06K9/00 ,  G06K9/36

Abstract: Systems and methods for retrieving, manipulating, and viewing 3D image objects from 3D virtual microscope slide images are provided. An image library module provides access to the imagery data in a 3D virtual slide and constructs 3D image objects that are coextensive with the 3D virtual slide or a 3D sub-portion thereof. From within the 3D image object, cross layer planar views spanning various depths of the 3D virtual slide are constructed as well as 3D prisms and other shaped image areas. The image library module allows a 3D image object to be sliced into horizontal and vertical views, skewed cross layer views and regular and irregular shaped 3D image areas for viewing by a user.

公开(公告)号：WO2004079636A1

公开(公告)日：2004-09-16

申请号：PCT/US2004/004314

申请日：2004-02-13

Applicant: APERIO TECHNOLOGIES, INC. ,  EICHHORN, Ole ,  SOENKSEN, Dirk, G.

Inventor： EICHHORN, Ole ,  SOENKSEN, Dirk, G.

IPC: G06K9/00

CPC classification number: G06K9/6218 ,  G06K9/00147 ,  G06T9/008 ,  G06T9/40 ,  H04N19/94 ,  H04N19/96

Abstract: Systems and methods for image pattern recognition comprise digital image capture and encoding using vector quantization ("VQ") of the image. A vocabulary of vectors is built by segmenting images into kernels and creating vectors corresponding to each kernel. Images are encoded by creating a vector index file having indices that point to the vectors stored in the vocabulary. The vector index file can be used to reconstruct an image by looking up vectors stored in the vocabulary. Pattern recognition of candidate regions of images can be accomplished by correlating image vectors to a pre-trained vocabulary of vector sets comprising vectors that correlate with particular image characteristics. In virtual microscopy, the systems and methods are suitable for rare-event finding, such as detection of micrometastasis clusters, tissue identification, such as locating regions of analysis for immunohistochemical assays, and rapid screening of tissue samples, such as histology sections arranged as tissue microarrays (TMAs).

Abstract translation: 用于图像模式识别的系统和方法包括使用图像的矢量量化（“VQ”）的数字图像捕获和编码。 通过将图像分割为内核并创建与每个内核相对应的向量来构建向量的词汇表。 通过创建具有指向存储在词汇表中的向量的索引的向量索引文件对图像进行编码。 向量索引文件可用于通过查找存储在词汇表中的向量来重建图像。 可以通过将图像向量与包括与特定图像特征相关的向量的预先训练的矢量集合的词汇相关联来实现图像候选区域的模式识别。 在虚拟显微镜中，系统和方法适用于罕见事件发现，例如检测微转移簇，组织鉴定，例如用于免疫组织化学测定的定位分析区域，以及组织样品的快速筛选，例如排列成组织的组织切片 微阵列（TMA）。

公开(公告)号：WO2014018114A1

公开(公告)日：2014-01-30

申请号：PCT/US2013/032328

申请日：2013-03-15

Applicant: APERIO TECHNOLOGIES, INC.

Inventor： WRENN, Mark ,  SOENKSEN, Dirk

IPC: G06K9/18 ,  G06K9/00

CPC classification number: G06F17/30 ,  G06F19/00 ,  G06Q10/10 ,  G06Q50/22 ,  H04N1/32133 ,  H04N2201/323 ,  H04N2201/3269 ,  H04N2201/3271

Abstract: Systems and methods for tracking a slide comprising a tissue sample. In an embodiment, data is received from a Laboratory Information System (LIS) barcode associated with a slide and a proprietary barcode generated by a slide staining system, such as a Ventana slide staining system, to track the slide through the staining process. A new label is printed that contains both the LIS barcode and the proprietary barcode, and is attached to the slide. When the slide is subsequently scanned by a slide scanning system, the slide scanning system reads and decodes the LIS barcode to obtain a LIS barcode value. The LIS barcode value may then be used to retrieve information associated with the slide.

Abstract translation: 用于跟踪包含组织样本的载玻片的系统和方法。 在一个实施例中，从与幻灯片相关联的实验室信息系统（LIS）条形码和由幻灯片染色系统（例如Ventana幻灯片染色系统）产生的专有条形码接收数据以跟踪通过染色过程的幻灯片。 打印包含LIS条形码和专有条形码的新标签，并附加到幻灯片。 当幻灯片随后通过幻灯片扫描系统扫描时，幻灯片扫描系统读取并解码LIS条形码以获得LIS条形码值。 然后可以使用LIS条形码值来检索与幻灯片相关联的信息。

公开(公告)号：WO2009039450A1

公开(公告)日：2009-03-26

申请号：PCT/US2008/077128

申请日：2008-09-19

Applicant: APERIO TECHNOLOGIES, INC. ,  SOENKSEN, Dirk G. ,  PERZ, Cindy

Inventor： SOENKSEN, Dirk G. ,  PERZ, Cindy

IPC: G06Q50/00

CPC classification number: G06K9/00127 ,  G02B21/241 ,  G06K9/0014 ,  G06T5/003 ,  G06T5/50 ,  G06T7/0012 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An improved diagnostic resolution of digital slide images is obtained by scanning a first digital slide image at diagnostic resolution that is then deconvolved into separate images with one stain per image. The single stain images are then enhanced with image adjustments and/or processed with image analysis algorithms. The resulting single image data sets from the image analysis algorithms can then be stored. Additionally, the resulting enhanced single images can be recombined into a second digital slide image at diagnostic resolution that is also enhanced.

Abstract translation: 通过以诊断分辨率扫描第一数字幻灯片图像来获得数字幻灯片图像的改进的诊断分辨率，然后将其解映射成具有每个图像的一个染色的分离的图像。 然后通过图像调整和/或用图像分析算法处理单个染色图像。 然后可以存储从图像分析算法得到的单个图像数据集。 此外，所得增强的单个图像可以以诊断分辨率重新组合成第二数字幻灯片图像，其也被增强。

公开(公告)号：WO2008005861A3

公开(公告)日：2008-01-10

申请号：PCT/US2007/072527

申请日：2007-06-29

Applicant: APERIO TECHNOLOGIES, INC. ,  EICHHORN, Ole

Inventor： EICHHORN, Ole

IPC: G06F17/30 ,  G06F7/00

Abstract: Images created by one or more image acquisition devices are initially stored in remote data bases at one or more remote or satellite locations and copies of the images are subsequently transferred to a central server at a central storage location over a network and stored in a central data base. A common catalog listing local image files stored at the central data base and remote image files stored at one or more remote data bases is stored at the central location and is accessed by users to search for images. User requests for images associated with local image files result in transfer of the requested images by the central server to the user. User requests for images associated with remote image files are relayed by the central server to the associated remote location, and the requested images are then relayed to the user from the remote location via the central server.

公开(公告)号：WO2007101205A2

公开(公告)日：2007-09-07

申请号：PCT/US2007/062890

申请日：2007-02-27

Applicant: APERIO TECHNOLOGIES, INC ,  CRANDALL, Greg J.

Inventor： CRANDALL, Greg J.

CPC classification number: G02B21/34 ,  G02B21/002 ,  G02B21/367

Abstract: Systems and methods for microscope slide scanning using multiple sensor arrays that receive imagery data from a single optical axis are provided. A single, high quality, easily obtained microscope objective lens is used to project an image onto two or more sensor arrays. The sensor arrays can be linear or two dimensional and imaging takes place along a single optical axis. Simultaneous sensor acquisition and parallel data processing reduce the image acquisition time by a factor of N, where N represents the number of sensors employed.

Abstract translation: 提供了使用从单个光轴接收图像数据的多个传感器阵列进行显微镜幻灯片扫描的系统和方法。 使用单个，高质量，容易获得的显微镜物镜将图像投影到两个或更多个传感器阵列上。 传感器阵列可以是线性的或二维的并且沿着单个光轴进行成像。 同时传感器采集和并行数据处理将图像采集时间缩短了N倍，其中N表示采用的传感器数量。

公开(公告)号：WO2005119325A3

公开(公告)日：2006-12-07

申请号：PCT/US2005018677

申请日：2005-05-26

Applicant: APERIO TECHNOLOGIES INC ,  OLSON ALLEN ,  SALIGRAMAL KIRAN ,  SOENKSEN DIRK G

Inventor： OLSON ALLEN ,  SALIGRAMAL KIRAN ,  SOENKSEN DIRK G

IPC: G06K9/00 ,  A61B8/02 ,  G01N27/72 ,  G02B7/04 ,  G02B21/36

CPC classification number: G06T7/0012 ,  G02B21/365 ,  G06K9/00127 ,  G06K9/036 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: Systems and methods for assessing virtual microscope slide image quality are provided. In order to determine whether a virtual slide image has any out of focus areas and is therefore a candidate for manual inspection, the various focus points used to scan the virtual slide image are used to calculate a best fit surface for the virtual slide image. The distance of each focus point from the best fit surface is then calculated and the largest distance is compared to a predetermined value. If the largest distance from a focus point to the best fit surface is larger than the predetermined value, then the virtual slide image is designated as needing a manual inspection and possible re-scan.

Abstract translation: 提供了评估虚拟显微镜幻灯片图像质量的系统和方法。 为了确定虚拟幻灯片图像是否具有任何偏离焦点区域，因此是用于手动检查的候选者，用于扫描虚拟幻灯片图像的各种对焦点用于计算虚拟幻灯片图像的最佳拟合曲面。 然后计算每个对焦点与最佳拟合表面的距离，并将最大距离与预定值进行比较。 如果从焦点到最佳配合曲面的最大距离大于预定值，则虚拟幻灯片图像被指定为需要手动检查和可能的重新扫描。

公开(公告)号：WO2005119575A3

公开(公告)日：2006-02-09

申请号：PCT/US2005018553

申请日：2005-05-27

Applicant: APERIO TECHNOLOGIES INC ,  EICHHORN OLE ,  CRANDALL GREG J ,  HASHAGEN STEVEN ,  SOENKSEN DIRK G ,  WRENN MARK

Inventor： EICHHORN OLE ,  CRANDALL GREG J ,  HASHAGEN STEVEN ,  SOENKSEN DIRK G ,  WRENN MARK

IPC: G02B21/34 ,  G02B21/36 ,  G06K9/00

CPC classification number: G02B27/2278 ,  G02B21/367 ,  G06K9/00201 ,  H04N13/0495

Abstract: Systems and methods for creating and viewing three dimensional virtual slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution (250). These two dimensional images are provided to an image viewing workstation where they are viewed by an operator (255) who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth (270). The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter (275).

Abstract translation: 提供了用于创建和查看三维虚拟幻灯片的系统和方法。 将一个或多个显微镜载玻片放置在图像采集装置中，扫描载玻片上的样本，并以中等或高分辨率（250）进行二维图像。 这些二维图像被提供给图像查看工作站，在那里它们被操作者（255）观看，所述操作者平移和缩放二维图像并选择感兴趣的区域以在多个深度级别（Z平面）进行扫描。 图像采集装置接收多个深度级扫描的一组参数，包括位置和深度（270）。 然后，图像获取装置在一系列Z平面图像中的位置处扫描样本，其中每个Z平面图像对应于深度参数（275）内的样本的深度级部分。