公开(公告)号：US10656140B2

公开(公告)日：2020-05-19

申请号：US15425253

申请日：2017-02-06

Applicant: POCARED Diagnostics LTD.

Inventor： Gal Ingber ,  William G. Atterbury ,  Russell H. Barnes ,  Douglas E. Boyd ,  Joseph D. Dennis ,  Jonathan Gurfinkel ,  Dave Holley ,  Steven E. Huckaby ,  Thomas A. Klausing ,  John S. Laudo ,  Kevin Sadeski ,  Jason A. Schaefer ,  K. Bryan Scott ,  Carol Stillman ,  Sherwood Talbert ,  John Tallarico

IPC: G01N35/02 ,  G01N33/493 ,  C12Q1/04 ,  G01N21/03 ,  G01N35/04 ,  G01N21/64

Abstract: A system for conducting the identification and quantification of micro-organisms, e.g., bacteria in urine samples which includes: 1) several disposable cartridges for holding four disposable components including a centrifuge tube, a pipette tip having a 1 ml volume, a second pipette tip having a 0.5 ml volume, and an optical cup or cuvette; 2) a sample processor for receiving the disposable cartridges and processing the urine samples including transferring the processed urine sample to the optical cups; and 3) an optical analyzer for receiving the disposable cartridges and configured to analyze the type and quantity of micro-organisms in the urine sample. The disposable cartridges with their components including the optical cups or cuvettes are used in the sample processor, and the optical cups or cuvettes containing the processed urine samples are used in the optical analyzer for identifying and quantifying the type of micro-organism existing in the processed urine samples.

公开(公告)号：US10130902B2

公开(公告)日：2018-11-20

申请号：US14560363

申请日：2014-12-04

Applicant: POCARED Diagnostics LTD.

Inventor： Jonathan Gurfinkel ,  Gal Ingber

IPC: B01D29/00 ,  B01D37/00 ,  B01L3/00 ,  C12Q1/24 ,  G01N1/40 ,  G01G17/06 ,  G01N33/493 ,  G01N33/49 ,  G01N5/02 ,  G01N15/00 ,  G01F15/12

Abstract: A filter arrangement with a top element and a bottom element and a filter element therebetween captures oversized particles on the upper surface of the filter element and tangentially rinses these particles using an elution fluid to provide a concentration of particles in a relatively low volume of fluid for further analysis. A configuration using a slider valve may also be utilized. Additionally, an arrangement of supply and receiving containers may be used to minimize the number of containers required. A mass flow meter may be incorporated to measure the flow of elution fluid. Finally, a wash stage of the filtering process may be used to introduce stain onto the particles for further analysis, such as that associated with Gram staining and these stained particles may be further analyzed.

公开(公告)号：US10018564B2

公开(公告)日：2018-07-10

申请号：US15060703

申请日：2016-03-04

Applicant: POCARED Diagnostics LTD.

Inventor： Jonathan Gurfinkel ,  Gal Ingber

IPC: G01N21/64 ,  C12Q1/04 ,  G01N33/483

CPC classification number: G01N21/6486 ,  C12Q1/04 ,  G01N33/4833 ,  G01N2021/6417 ,  G01N2201/062 ,  G01N2201/129

Abstract: This invention relates to a method that allows for the differentiation of isolates of commonly encountered bacteria that contain specific antibiotic-resistance genes from similar isolates that do not harbor the gene. More particularly, the invention relates to a method that utilizes an automated rapid platform system that employs intrinsic fluorescence, optical data analysis, and artificial intelligence methods to analyze multi-dimensional optical characteristics of bacterial strains.

公开(公告)号：US20170023480A1

公开(公告)日：2017-01-26

申请号：US15060703

申请日：2016-03-04

Applicant: POCARED Diagnostics LTD.

Inventor： Jonathan Gurfinkel ,  Gal Ingber

IPC: G01N21/64 ,  C12Q1/04 ,  G01N33/483

CPC classification number: G01N21/6486 ,  C12Q1/04 ,  G01N33/4833 ,  G01N2021/6417 ,  G01N2201/062 ,  G01N2201/129

Abstract: This invention relates to a method that allows for the differentiation of isolates of commonly encountered bacteria that contain specific antibiotic-resistance genes from similar isolates that do not harbor the gene. More particularly, the invention relates to a method that utilizes an automated rapid platform system that employs intrinsic fluorescence, optical data analysis, and artificial intelligence methods to analyze multi-dimensional optical characteristics of bacterial strains.

Abstract translation: 本发明涉及允许从不含有该基因的类似分离物中分离含有特定抗生素抗性基因的常见细菌的分离物的方法。 更具体地，本发明涉及利用采用固有荧光，光学数据分析和人工智能方法来分析细菌菌株的多维光学特性的自动化快速平台系统的方法。

公开(公告)号：US20150010993A1

公开(公告)日：2015-01-08

申请号：US14330450

申请日：2014-07-14

Applicant: POCARED Diagnostics LTD.

Inventor： Gal Ingber ,  William G. Atterbury ,  Russell H. Barnes ,  Douglas E. Boyd ,  Joseph D. Dennis ,  Jonathan Gurfinkel ,  Dave Holley ,  Steven E. Huckaby ,  Thomas A. Klausing ,  Kevin Sadeski ,  Jason A. Schaefer ,  K. Bryan Scott ,  Carol Stillman ,  Sherwood Talbert ,  John Tallarico ,  John S. Laudo

IPC: G01N33/493 ,  G01N35/02 ,  G01N21/03

CPC classification number: G01N33/493 ,  C12Q1/04 ,  G01N21/0303 ,  G01N35/025 ,  G01N35/04 ,  G01N2021/6482 ,  G01N2035/0436 ,  G01N2035/0444 ,  G01N2035/0448 ,  G01N2035/0458 ,  G01N2201/0415

Abstract: A system for conducting the identification and quantification of micro-organisms, e.g., bacteria in urine samples which includes: 1) several disposable cartridges for holding four disposable components including a centrifuge tube, a pipette tip having a 1 ml volume, a second pipette tip having a 0.5 ml volume, and an optical cup or cuvette; 2) a sample processor for receiving the disposable cartridges and processing the urine samples including transferring the processed urine sample to the optical cups; and 3) an optical analyzer for receiving the disposable cartridges and configured to analyze the type and quantity of micro-organisms in the urine sample. The disposable cartridges with their components including the optical cups or cuvettes are used in the sample processor, and the optical cups or cuvettes containing the processed urine samples are used in the optical analyzer for identifying and quantifying the type of micro-organism existing in the processed urine samples.

Abstract translation: 用于进行微生物识别和定量的系统，例如尿样中的细菌，其包括：1）几个用于保持四个一次性组件的一次性盒，包括离心管，具有1ml体积的移液管尖端，第二移液管尖 具有0.5ml体积，以及光学杯或比色皿; 2）用于接收一次性盒并且处理尿液样品的样品处理器，包括将经处理的尿样转移到光学杯; 以及3）用于接收一次性盒并用于分析尿样中的微生物的类型和数量的光学分析仪。 在样品处理器中使用具有包括光学杯或比色皿的组件的一次性盒，并且在光学分析仪中使用含有经处理的尿液样品的光学杯或比色杯，用于鉴定和定量存在于加工过程中的微生物的类型 尿样。

公开(公告)号：US20140246389A1

公开(公告)日：2014-09-04

申请号：US14172491

申请日：2014-02-04

Applicant: POCARED Diagnostics LTD.

Inventor： Gal Ingber

IPC: B01D35/28 ,  G01N1/34

CPC classification number: G01N1/34 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/049 ,  G01N1/4077 ,  G01N2001/4088

Abstract: A filter arrangement with a top element and a bottom element and a filter element therebetween captures oversized particles on the upper surface of the filter element and tangentially rinses these particles using an elution fluid to provide a concentration of particles in a relatively low volume of fluid for further analysis. In an intermediate step, the particles captured by the filter may be rinsed with a rinsing fluid such as water to pass additional undersized particles through the filter, thereby providing a purer sample. To improve efficiency, check valves may be used for passageways with one-way flow. Additionally, a configuration of three-way stopcocks may also be utilized. Finally, a sandwich arrangement is possible, wherein a single bottom element is sandwiched between two opposing top elements.

Abstract translation: 具有顶部元件和底部元件以及过滤元件的过滤器装置在过滤器元件的上表面上捕获过大的颗粒，并使用洗脱流体切向冲洗这些颗粒，以提供相对低体积的流体中的颗粒浓度 进一步分析。 在中间步骤中，由过滤器捕获的颗粒可以用诸如水的冲洗液冲洗以通过另外的过小的颗粒通过过滤器，从而提供更纯的样品。 为了提高效率，止回阀可用于单向流动的通道。 另外，也可以使用三通旋塞的构造。 最后，三明治布置是可能的，其中单个底部元件夹在两个相对的顶部元件之间。

公开(公告)号：US10905978B2

公开(公告)日：2021-02-02

申请号：US16161685

申请日：2018-10-16

Applicant: POCARED Diagnostics LTD.

Inventor： Jonathan Gurfinkel ,  Gal Ingber

IPC: B01D29/00 ,  G01N1/30 ,  B01D37/00 ,  G01N1/40 ,  G01N1/28 ,  G01G17/06 ,  B01L3/00 ,  G01N33/493 ,  C12Q1/24 ,  G01N5/02 ,  G01N15/00 ,  G01F15/12

Abstract: A filter arrangement with a top element and a bottom element and a filter element therebetween captures oversized particles on the upper surface of the filter element and tangentially rinses these particles using an elution fluid to provide a concentration of particles in a relatively low volume of fluid for further analysis. A configuration using a slider valve may also be utilized. Additionally, an arrangement of supply and receiving containers may be used to minimize the number of containers required. A mass flow meter may be incorporated to measure the flow of elution fluid. Finally, a wash stage of the filtering process may be used to introduce stain onto the particles for further analysis, such as that associated with Gram staining and these stained particles may be further analyzed.

公开(公告)号：US20190285661A1

公开(公告)日：2019-09-19

申请号：US16361766

申请日：2019-03-22

Applicant: POCARED Diagnostics LTD.

Inventor： Gal Ingber ,  William G. Atterbury ,  Dave Holley ,  Thomas A. Klausing ,  John S. Laudo ,  Jason A. Schaefer ,  Sherwood Talbert ,  John Tallarico

IPC: G01N35/02 ,  B04B9/14

Abstract: The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria in biological samples. More particularly, the invention relates to a system comprising a cooling, heating and fan arrangement for maintaining a predetermined optimum temperature of the samples during testing; a visual, circumferential and axial alignment system for aligning the samples within the carousel; a transfer system for transferring the samples from the carousel to the centrifuge; a balancing system of minimizing the rotational vibrations of the centrifuge; a safety system and anti-tipping design for the sample containing system; liquid dispensing arms for dispensing the buffered saline solution; and discharge ports for discharging and disposing of the liquid removed from the samples to a location external of the system.

公开(公告)号：US20190017090A1

公开(公告)日：2019-01-17

申请号：US16033963

申请日：2018-07-12

Applicant: POCARED Diagnostics LTD.

Inventor： Moshe Ben-David ,  Eran Zahavy ,  Gal Ingber

IPC: C12Q1/06

Abstract: Single dye fluorescent staining and the combination of differences in both intensity and spectral emission permit determination of the minimum concentration of an antibiotic needed to inactivate bacteria (Minimum Inhibitory Concentration (MIC)), thereby providing a means for rapid Antibiotic Susceptibility Testing (AST). This allows for a quick and easy means for clinicians to determine a suitable treatment regimen for patients suffering from bacterial infections and those that eventually lead to sepsis.

公开(公告)号：US20180080000A1

公开(公告)日：2018-03-22

申请号：US15824538

申请日：2017-11-28

Applicant: POCARED DIAGNOSTICS LTD.

Inventor： Gal Ingber ,  Martha J. Rogers

IPC: C12M1/34 ,  G01N21/03 ,  B01L3/00 ,  G01N21/64 ,  G01N35/04

Abstract: The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria, in biological samples. More particularly, the invention relates to a system comprising a disposable cartridge and an optics cup or cuvette having a tapered surface; wherein the walls are angled to allow for better coating and better striations of the light. The system may utilize the disposable cartridge in the sample processor and the optics cup or cuvette in the optical analyzer, wherein the optics cup also has a floor in the shape of an inverted arch.