公开(公告)号：WO1996005500A1

公开(公告)日：1996-02-22

申请号：PCT/US1995009926

申请日：1995-08-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OBREMSKI, Robert, J. ,  SILZEL, John, W.

IPC: G01N21/64

CPC classification number: G01N21/314 ,  G01J3/4338 ,  G01N21/64 ,  G01N2201/1293

Abstract: A method and apparatus determines the analyte content of a sample by generating first and second input signals and directing the input signals to the sample. The input signals differ in wavelength by at least 3 nanometers. Due to the interaction between the input signals and the sample, first and second output signals are generated. Each output signal comprises a resonant signal whose peak wavelength is substantially independent of the wavelength of the respective input signal, and a non-resonant output signal whose peak wavelength is dependent upon the wavelength of respective input signal. A detector is used to detect the two output signals, and by distinguishing the resonant output signals from the non-resonant output signals, data about the analyte content of the sample is determined. Principal components regression analysis or multivariate quantitative analysis can be applied to the output signals, for the purpose of distinguishing between the resonant and non-resonant signals. The method and apparatus can also distinguish resonant output signals from each other, and non-resonant output signals from each other.

Abstract translation: 方法和装置通过产生第一和第二输入信号并将输入信号引导到样品来确定样品的分析物含量。 输入信号波长不同至少3纳米。 由于输入信号和采样之间的相互作用，产生了第一和第二输出信号。 每个输出信号包括峰值波长基本上与各个输入信号的波长无关的谐振信号，以及峰值波长取决于相应输入信号的波长的非谐振输出信号。 检测器用于检测两个输出信号，并且通过区分谐振输出信号与非谐振输出信号，确定关于样品的分析物含量的数据。 主成分回归分析或多变量定量分析可以应用于输出信号，以区分谐振和非谐振信号。 该方法和装置还可以区分彼此的谐振输出信号和彼此之间的非谐振输出信号。

公开(公告)号：WO1996004555A1

公开(公告)日：1996-02-15

申请号：PCT/US1995009476

申请日：1995-07-27

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHENG, Anthony, K. ,  KIM, Julie, S. ,  OH, Chan, S.

IPC: G01N33/53

CPC classification number: G01N33/54393 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/817 ,  Y10S436/826 ,  Y10S436/909

Abstract: Methods for modulating the rates and dose responses of immunoassays through the incorporation of one or more detergents into the immunoassay reaction are disclosed. The methods are particularly suitable for automated immunoassay formats, especially with formats that use analyte-biotin bidentate reagents. The methods may be used to facilitate the detection of any desired, preselected pharmacological agent.

Abstract translation: 公开了通过将一种或多种洗涤剂并入免疫测定反应来调节免疫测定的速率和剂量反应的方法。 该方法特别适用于自动化免疫分析方法，特别是使用分析物 - 生物素二齿试剂的方法。 所述方法可用于促进检测任何所需的预选药理剂。

公开(公告)号：WO1996001697A1

公开(公告)日：1996-01-25

申请号：PCT/US1995008557

申请日：1995-07-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SRINIVASAN, Krupa, S.

IPC: B04B13/00

CPC classification number: B04B13/003

Abstract: A centrifuge system (10) and method includes generating a radio frequency excitation field within a housing (32) containing a rotor (16) of interest. The excitation field may be generated by an exciter coil (37) fixed to the cover (34) of the housing. The rotor includes a locking knob (23) that encloses a receiver coil (25) inductively coupled to the exciter coil. The excitation field causes current flow through the receiver coil. The current is rectified and used to power encoding circuitry. The encoding circuitry produces a modulated signal unique to the rotor or to a model in which the rotor is classified. The encoded signal is transmitted from within the locking knob to a reader coil (34) connected to the housing of the centrifuge. The reader coil receives the encoded signal, whereafter the signal is decoded and used to identify the rotor or rotor model.

Abstract translation: 离心机系统（10）和方法包括在包含感兴趣的转子（16）的壳体（32）内产生射频激励场。 励磁场可以由固定到壳体的盖（34）的激励器线圈（37）产生。 转子包括锁定旋钮（23），该锁定旋钮包围感应地耦合到激励器线圈的接收器线圈（25）。 励磁场引起电流流过接收器线圈。 电流被整流并用于对编码电路供电。 编码电路产生对转子独特的调制信号或转子分类的模型。 编码信号从锁定旋钮内传送到连接到离心机壳体的读取器线圈（34）。 读取器线圈接收编码信号，此后信号被解码并用于识别转子或转子模型。

公开(公告)号：WO1996001695A1

公开(公告)日：1996-01-25

申请号：PCT/US1995008507

申请日：1995-07-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SCHENCK, Alan, L. ,  SONG, Jin, Y.

IPC: B04B13/00

CPC classification number: B04B15/08 ,  B04B13/003 ,  B04B15/02 ,  G01N2001/4027 ,  G01N2035/00495

Abstract: A method and system of determining an operation parameter for a centrifuge includes measuring one or more physical characteristics of a rotor interchangeably supported by a rotor drive. In the preferred embodiment, windage is measured at a first rotational speed and a speed-dependent signal is generated to adjust a vacuum system based upon changes in rotational speed of the rotor. Changes in windage may also be used as a basis for adjusting a refrigeration circuit. Either as an alternative or in addition to monitoring windage, the moment of inertia can be measured and used to adjust adaptive circuitry. For example, the drive circuitry may be adjusted based upon a determination of inertia as the physical characteristic of interest. The method and system isolate inertia and windage as the main forms of resistance to acceleration of the rotor.

Abstract translation: 确定离心机的操作参数的方法和系统包括测量由转子驱动器可互换地支撑的转子的一个或多个物理特性。 在优选实施例中，以第一旋转速度测量风速，并且产生基于速度的信号，以基于转子的转速的变化来调节真空系统。 风阻变化也可以用作调节制冷回路的基础。 作为监测风向的替代或补充，惯性矩可以被测量并用于调整自适应电路。 例如，可以基于惯性的确定来调整驱动电路作为感兴趣的物理特性。 该方法和系统将惯性和风阻作为转子加速阻力的主要形式。

公开(公告)号：WO1995032428A1

公开(公告)日：1995-11-30

申请号：PCT/US1995006367

申请日：1995-05-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  YAN, Cheng, F. ,  OH, Chan, S. ,  CHENG, Anthony, K.

IPC: G01N33/543

CPC classification number: G01N33/532 ,  G01N33/58 ,  G01N33/94 ,  Y10S436/805 ,  Y10S436/815 ,  Y10S436/816

Abstract: Bidentate reagents for rapidly and quantitatively assaying the concentration of pharmacological agents in biological samples are described. The reagents are used in an immunoassay format for determining the concentration of desired, preselected pharmacological agents, such as benzoylecgonine, cocaine, an opiate, PCP, digoxigenin, acetaminophen, carbamazepine, phenytoin, primidone, theophylline, an aminoglycoside antibiotic, vancomycin, quinidine or a cannabinoid.

Abstract translation: 描述了用于快速和定量测定生物样品中药理学剂浓度的二齿试剂。 试剂以免疫测定方式用于测定所需预选的药理学试剂如苯甲酰扁桃酸，可卡因，阿片剂，PCP，地高辛，对乙酰氨基酚，卡马西平，苯妥英，扑米酮，茶碱，氨基糖苷类抗生素，万古霉素，奎尼丁或奎宁 一种大麻素。

公开(公告)号：WO1995021270A2

公开(公告)日：1995-08-10

申请号：PCT/US1995001468

申请日：1995-02-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W.

IPC: C12Q01/68

CPC classification number: C12Q1/686 ,  C12Q1/6862

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

Abstract translation: 公开了靶核酸分子的检测和指数扩增的方法和试剂。 该方法通常使用引物寡核苷酸，其与目标分子的链上的阻断剂寡核苷酸和可以与阻断剂寡核苷酸杂交的末端寡核苷酸一起杂交。

公开(公告)号：WO1995011748A1

公开(公告)日：1995-05-04

申请号：PCT/US1994012443

申请日：1994-10-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MATSON, Robert, S. ,  COASSIN, Peter, J. ,  RAMPAL, Jang, B. ,  SOUTHERN, Edwin, M.

IPC: B01J19/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00722 ,  C07K1/045 ,  C12Q1/6874 ,  C40B40/06 ,  C40B60/14 ,  G01N35/1072

Abstract: An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface (56) whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator (10) in the form of a thick plate (12) having in a surface (16) at least one cavity in the form of an open chamber or channel (14) is used for applying reagents to the surface (56) of a solid phase support material. The applicator (10) is positioned over the solid support material (56) with the surface having the cavity (14) sealed against the material surface. For each cavity (14), reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material (56), where each element of the array contains a population of biopolymers having identical sequence. Further synthesis of additional biopolymers is performed by linearly moving or rotating the applicator (10) relative to the support material (56) and carrying out synthesis procedure so that another one-dimensional matrix or array of biopolymers are formed at an offset position to the previous array. At each overlapping region of the two arrays, a cell of biopolymers are formed which each comprises a strand from the first array and a strand from the second array. The result is a two-dimensional array of biopolymers of different sequences at each discrete cell. The arrays of discrete cell containing different biopolymers may be used to conduct hybridization reaction analysis of a biological sample.

Abstract translation: 一种用于在二维支撑表面（56）上进行生物聚合物合成的自动化方法和装置，由此在表面上获得二维基质或生物聚合物阵列。 形式为厚板（12）的施加器（10），其具有在开口腔或通道（14）形式的至少一个空腔的表面（16）中，用于将试剂施加到表面（56）的表面 固相支撑材料。 涂抹器（10）定位在固体支撑材料（56）上方，具有空腔（14）的表面密封在材料表面上。 对于每个空腔（14），合成试剂依次导入空腔的一端并从空腔的另一端收集。 因此，在载体材料（56）上形成一维生物聚合物阵列，其中阵列的每个元件含有具有相同序列的生物聚合物群。 通过相对于支撑材料（56）线性移动或旋转施加器（10）并执行合成程序来进行另外的生物聚合物的进一步合成，使得另一个一维矩阵或生物聚合物阵列形成在与之前的 阵列。 在两个阵列的每个重叠区域，形成生物聚合物的细胞，每个细胞包含来自第一阵列的链和来自第二阵列的链。 其结果是在每个离散的细胞上具有不同序列的生物聚合物的二维阵列。 含有不同生物聚合物的离散细胞阵列可用于进行生物样品的杂交反​​应分析。

公开(公告)号：WO1995011451A1

公开(公告)日：1995-04-27

申请号：PCT/US1994011802

申请日：1994-10-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHIEH, Chia-Hui

IPC: G01N27/447

CPC classification number: G01N27/44752

Abstract: Methods for controlling and varying electroosmotic flow in coated capillary electrophoresis columns under electrophoretic conditions are disclosed. The methods described herein involve varying the concentration of a multi-valent buffer compound in electrophoresis buffer compositions in order to control the electroosmotic flow in capillary columns having interior surfaces coated with charged organic coatings. Increasing the concentration of a multi-valent buffer compound which has a charge opposite the charge of the organic coating, in the electrophoresis buffer results in a decrease in electroosmotic flow. Decreasing the concentration of a multi-valent buffer compound results in an increase in electroosmotic flow. The ability to control electroosmotic flow provides enhanced resolution and optimizes separations of electrophoresis sample components.

Abstract translation: 公开了在电泳条件下在涂布的毛细管电泳柱中控制和改变电渗流的方法。 本文描述的方法涉及改变电泳缓冲液组合物中多价缓冲剂化合物的浓度，以便控制具有涂覆带电有机涂层的内表面的毛细管柱中的电渗流。 在电泳缓冲液中增加具有与有机涂层电荷相反的电荷的多价缓冲化合物的浓度导致电渗流减少。 减少多价缓冲剂化合物的浓度导致电渗流量的增加。 控制电渗流量的能力提高了分辨率，优化了电泳样品组分的分离。

公开(公告)号：WO1995009360A1

公开(公告)日：1995-04-06

申请号：PCT/US1994010981

申请日：1994-09-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHIEH, Chia-Hui

IPC: G01N27/447

CPC classification number: G01N27/44752

Abstract: Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface coated with at least one cross-linked organic multivalent ionic compound capable of ionic interaction with charged chemical functionalities on the tubing interior surface. Exemplary columns are prepared by cross-linking a multi-functional amine compound with a diepoxide, alkylating the cross-linked multi-functional amine compound to form a cross-linked quaternary ammonium compound, and then contacting the interior surface of capillary tubing with the cross-linked quaternary ammonium compound.

Abstract translation: 公开了涂布毛细管电泳柱及其用于电泳分离的方法。 涂覆的毛细管柱包括长度的管，其具有涂覆有至少一种能够在管内表面上与带电化学官能团进行离子相互作用的交联的有机多价离子化合物的内表面。 通过将多官能胺化合物与二环氧化物交联，烷基化交联的多官能胺化合物以形成交联的季铵化合物，然后使毛细管内表面与十字 连接的季铵化合物。

公开(公告)号：WO1994016330A1

公开(公告)日：1994-07-21

申请号：PCT/US1993012140

申请日：1993-12-13

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OH, Chan, S. ,  CHENG, Anthony, K. ,  MICHAEL, Josephine, M. ,  DOBASHI, Thomas, S.

IPC: G01N33/531

CPC classification number: G01N33/532 ,  C07H15/234 ,  G01N33/531 ,  Y10S436/816 ,  Y10S436/817

Abstract: A method for making a preconjugate comprising an immunogenic species of a polymorphic analyte is disclosed. The method is carried out by reacting an activated binding moiety, and a polymorphic analyte at room temperature for between about 10 hours and about 60 hours. The attaching reaction results in an excess of the preconjugate comprising the immunogenic species of the polymorphic analyte. The preconjugate can be used to make an immunoreactive conjugate useful as a developer antigen in a competitive inhibition immunoassay for the polymorphic analyte.

Abstract translation: 公开了一种制备包含多态性分析物的免疫原性物质的预缀合物的方法。 该方法通过使活化的结合部分和多晶型分析物在室温下反应约10小时至约60小时来进行。 附着反应导致包含多态性分析物的免疫原性物质的预结合物过量。 预缀合物可用于在多态性分析物的竞争性抑制免疫测定中使免疫反​​应性缀合物用作显影剂抗原。