公开(公告)号：WO1995011748A1

公开(公告)日：1995-05-04

申请号：PCT/US1994012443

申请日：1994-10-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MATSON, Robert, S. ,  COASSIN, Peter, J. ,  RAMPAL, Jang, B. ,  SOUTHERN, Edwin, M.

IPC: B01J19/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00722 ,  C07K1/045 ,  C12Q1/6874 ,  C40B40/06 ,  C40B60/14 ,  G01N35/1072

Abstract: An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface (56) whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator (10) in the form of a thick plate (12) having in a surface (16) at least one cavity in the form of an open chamber or channel (14) is used for applying reagents to the surface (56) of a solid phase support material. The applicator (10) is positioned over the solid support material (56) with the surface having the cavity (14) sealed against the material surface. For each cavity (14), reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material (56), where each element of the array contains a population of biopolymers having identical sequence. Further synthesis of additional biopolymers is performed by linearly moving or rotating the applicator (10) relative to the support material (56) and carrying out synthesis procedure so that another one-dimensional matrix or array of biopolymers are formed at an offset position to the previous array. At each overlapping region of the two arrays, a cell of biopolymers are formed which each comprises a strand from the first array and a strand from the second array. The result is a two-dimensional array of biopolymers of different sequences at each discrete cell. The arrays of discrete cell containing different biopolymers may be used to conduct hybridization reaction analysis of a biological sample.

Abstract translation: 一种用于在二维支撑表面（56）上进行生物聚合物合成的自动化方法和装置，由此在表面上获得二维基质或生物聚合物阵列。 形式为厚板（12）的施加器（10），其具有在开口腔或通道（14）形式的至少一个空腔的表面（16）中，用于将试剂施加到表面（56）的表面 固相支撑材料。 涂抹器（10）定位在固体支撑材料（56）上方，具有空腔（14）的表面密封在材料表面上。 对于每个空腔（14），合成试剂依次导入空腔的一端并从空腔的另一端收集。 因此，在载体材料（56）上形成一维生物聚合物阵列，其中阵列的每个元件含有具有相同序列的生物聚合物群。 通过相对于支撑材料（56）线性移动或旋转施加器（10）并执行合成程序来进行另外的生物聚合物的进一步合成，使得另一个一维矩阵或生物聚合物阵列形成在与之前的 阵列。 在两个阵列的每个重叠区域，形成生物聚合物的细胞，每个细胞包含来自第一阵列的链和来自第二阵列的链。 其结果是在每个离散的细胞上具有不同序列的生物聚合物的二维阵列。 含有不同生物聚合物的离散细胞阵列可用于进行生物样品的杂交反​​应分析。