公开(公告)号：US20170226159A1

公开(公告)日：2017-08-10

申请号：US15514668

申请日：2015-09-25

Applicant: KANEKA CORPORATION ,  AnaSpec, Inc.

Inventor： Keishi TAKATSU ,  Hirofumi MAEDA ,  Akio FUJII ,  Fengying LI ,  Xoapbing WANG

IPC: C07K14/00 ,  C07D207/46 ,  B01D15/08 ,  C07C69/712 ,  C07K7/06 ,  C07K1/16 ,  C07K1/04

CPC classification number: C07K14/001 ,  B01D15/08 ,  C07C69/712 ,  C07D207/46 ,  C07K1/04 ,  C07K1/14 ,  C07K1/16 ,  C07K7/06

Abstract: A method for producing a purified peptide from a supported crude peptide having a support and a first peptide chain bonded to the support at the C-terminus. The method includes: introducing a linker and a hydrophilic unit to an amino group of the first peptide chain of the supported crude peptide; cleaving a bond between the first peptide chain and the support before or after at least one of the linker and the hydrophilic unit is introduced to the amino group of the first peptide chain such that a support-free hydrophilized peptide is obtained; treating the support-free hydrophilized peptide by liquid chromatography; and cleaving a bond between the linker and the first peptide chain in the support-free hydrophilized peptide by chemical treatment after the liquid chromatography treatment such that a peptide including the first peptide chain is obtained.

公开(公告)号：WO2010090756A2

公开(公告)日：2010-08-12

申请号：PCT/US2010000341

申请日：2010-02-05

Applicant: ANASPEC INC ,  HONG ANITA ,  RAKHMANOVA VERA ,  TONG XIAO-HE

Inventor： HONG ANITA ,  RAKHMANOVA VERA ,  TONG XIAO-HE

CPC classification number: G01N33/542 ,  C07K7/06 ,  G01N33/582 ,  G01N2333/95

Abstract: The present invention is generally directed to biological assays. More specifically it is directed to FRET-based assays using particularly effecting FRET pairs, methods for performing such assays and the molecules utilized in the assays. In a composition aspect, the present invention is directed to a FRET-based protease substrate selected from the list of substrates shown in the Detailed Description section above. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET-substrate is selected from the list of substrates shown in the Detailed Description section above. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description section above.

Abstract translation: 本发明一般涉及生物测定。 更具体地，其涉及使用特别有效的FRET对的FRET-测定法，用于进行此类测定的方法和用于测定中的分子。 在组合物方面，本发明涉及选自上述详细描述部分中所示的基材列表的基于FRET的蛋白酶底物。 在方法方面，本发明涉及一种进行基于FRET的测定法，其中测定法包括以下步骤：向反应容器中加入含有一种或多种不同蛋白质的溶液或悬浮液; 向反应容器中加入包含FRET-底物的液体，其中FRET-基材选自上述详细描述部分所示的基材列表。 在试剂盒方面，本发明涉及用于进行基于FRET的测定的试剂盒，其中所述试剂盒包含蛋白酶底物。 蛋白酶底物选自上述详细描述部分中所示的底物列表。

公开(公告)号：WO2010126600A1

公开(公告)日：2010-11-04

申请号：PCT/US2010/001268

申请日：2010-04-30

Applicant: ANASPEC, INC. ,  HONG, Anita ,  RAKHMANOVA, Vera

Inventor： HONG, Anita ,  RAKHMANOVA, Vera

IPC: C12Q1/37 ,  C12N9/50

CPC classification number: C12N9/50

Abstract: The present invention is generally directed to biological assays. More specifically it is directed to optimal FRET pairs and methods related to their use. In a composition aspect, the present invention is directed to FRET-based protease substrates selected from the list of substrates shown in the Detailed Description. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET substrate is selected from the list of substrates shown in the Detailed Description. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description.

Abstract translation: 本发明一般涉及生物测定。 更具体地说，它是针对与其使用相关的最佳FRET对和方法。 在组合物方面，本发明涉及从详细描述中所示的基材列表中选择的基于FRET的蛋白酶底物。 在方法方面，本发明涉及一种进行基于FRET的测定法，其中测定法包括以下步骤：向反应容器中加入含有一种或多种不同蛋白质的溶液或悬浮液; 向反应容器中加入包含FRET-底物的液体，其中FRET基材选自具体实施方式中所示的基材列表。 在试剂盒方面，本发明涉及用于进行基于FRET的测定的试剂盒，其中所述试剂盒包含蛋白酶底物。 蛋白酶底物选自详细描述中所示的底物列表。

公开(公告)号：WO2010090756A3

公开(公告)日：2010-08-12

申请号：PCT/US2010/000341

申请日：2010-02-05

Applicant: ANASPEC, INC. ,  HONG, Anita ,  RAKHMANOVA, Vera ,  TONG, Xiao-He

Inventor： HONG, Anita ,  RAKHMANOVA, Vera ,  TONG, Xiao-He

IPC: A61K38/04 ,  A61K35/14

Abstract: Methods for performing FRET-based biological assays using optimized FRET pairs, said methods comprise adding a solution or suspension containing one or more different proteins to a reaction vessel, adding a liquid comprising a FRET-based protease substrate to the reaction vessel Kit for performing a FRET-based assay, wherein the kit comprises a protease substrate.

公开(公告)号：WO2006047452A3

公开(公告)日：2006-09-14

申请号：PCT/US2005038259

申请日：2005-10-21

Applicant: ANASPEC INC ,  DIWU ZHENJUN ,  ZHANG JIANHENG ,  TANG YI ,  XIANG GUOBING

Inventor： DIWU ZHENJUN ,  ZHANG JIANHENG ,  TANG YI ,  XIANG GUOBING

IPC: A61K31/405 ,  C07D209/04

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3'-position, their bioconjugates and their uses are described. 1,3'-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1'-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：WO2006047452A2

公开(公告)日：2006-05-04

申请号：PCT/US2005/038259

申请日：2005-10-21

Applicant: ANASPEC, INC. ,  DIWU, Zhenjun ,  ZHANG, Jianheng ,  TANG, Yi ,  XIANG, Guobing

Inventor： DIWU, Zhenjun ,  ZHANG, Jianheng ,  TANG, Yi ,  XIANG, Guobing

IPC: C12Q1/68 ,  G01N33/00 ,  C07F15/00 ,  C07D487/22 ,  C07D498/22

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3’-position, their bioconjugates and their uses are described. 1,3’-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1’-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'位之间分子内交联的化学活性羰花青染料，它们的生物共轭物及其用途。 1,3'-交联的羰花青优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：EP3199540A1

公开(公告)日：2017-08-02

申请号：EP15844011.5

申请日：2015-09-25

Applicant: Kaneka Corporation ,  Anaspec, Inc.

Inventor： TAKATSU, Keishi ,  MAEDA, Hirofumi ,  FUJII, Akio ,  LI, Fengying ,  WANG, Xiaobing

IPC: C07K1/14 ,  C07K1/04

CPC classification number: C07K1/04 ,  C07K1/14

Abstract: An object of the present invention is to provide a producing method: that allows any hydrophobic peptide to be used as an object to be purified, in hydrophilizing a hydrophobic peptide, synthesized by solid-phase peptide synthesis, with a hydrophilic unit and purifying the hydrophobic peptide by HPLC, regardless of the type of the amino acid residue at the N-terminus; that allows the hydrophilic unit to be flexibly selected in accordance with the type of the hydrophobic peptide; and that is excellent in versatility.
The present invention is characterized by a method for producing a purified peptide from a supported crude peptide including a support and a first peptide chain bonded to the support at a C-terminus, the method comprising:
a hydrophilization step A of introducing a linker and a hydrophilic unit to an amino group of the supported crude peptide in this order stepwise or at a single step to obtain a support-free hydrophilized peptide;
a support cleavage step B of cleaving a bond between the first peptide chain and the support at any stage before bonding the linker to the supported crude peptide until the hydrophilized peptide is obtained, or after a supported hydrophilized peptide is obtained;
a chromatographic purification step of treating the support-free hydrophilized peptide obtained by the hydrophilization step A and the support cleavage step B, by liquid chromatography; and
a linker cleavage step of cleaving a bond between the linker and the first peptide chain included in the chromatographically-purified support-free hydrophilized peptide, by chemical treatment.

Abstract translation: 本发明的一个目的是提供一种制备方法：使任何疏水性肽用作待纯化的目标，将通过固相肽合成合成的疏水性肽与亲水性单元亲水化并纯化疏水性肽 无论N-末端的氨基酸残基的种类如何， 这允许亲水单元根据疏水肽的类型灵活地选择; 这在多功能性方面非常出色。 本发明的特征在于一种由载体上的粗肽制备纯化的肽的方法，所述肽包含载体和在C端与载体结合的第一肽链，所述方法包括：亲水化步骤A， 亲水单元逐步或在一个步骤中依次转化为载体粗肽的氨基以获得无载体亲水化肽; 在将接头连接到负载的粗肽上直到获得亲水化肽之前或在获得负载亲水化肽后，在任何阶段将第一肽链和支持物之间的键裂解的支持物裂解步骤B; 通过液相色谱法处理通过亲水化步骤A和载体裂解步骤B获得的无载体亲水化肽的色谱纯化步骤; 以及通过化学处理切断色谱纯化的无载体亲水化肽中包含的接头和第一肽链之间的键的接头断裂步骤。

公开(公告)号：EP3199540A4

公开(公告)日：2018-05-09

申请号：EP15844011

申请日：2015-09-25

Applicant: KANEKA CORP ,  ANASPEC INC

Inventor： TAKATSU KEISHI ,  MAEDA HIROFUMI ,  FUJII AKIO ,  LI FENGYING ,  WANG XIAOBING

IPC: C07K1/14 ,  C07K1/04

CPC classification number: C07K1/04 ,  C07K1/14

Abstract: An object of the present invention is to provide a producing method: that allows any hydrophobic peptide to be used as an object to be purified, in hydrophilizing a hydrophobic peptide, synthesized by solid-phase peptide synthesis, with a hydrophilic unit and purifying the hydrophobic peptide by HPLC, regardless of the type of the amino acid residue at the N-terminus; that allows the hydrophilic unit to be flexibly selected in accordance with the type of the hydrophobic peptide; and that is excellent in versatility. The present invention is characterized by a method for producing a purified peptide from a supported crude peptide including a support and a first peptide chain bonded to the support at a C-terminus, the method comprising: a hydrophilization step A of introducing a linker and a hydrophilic unit to an amino group of the supported crude peptide in this order stepwise or at a single step to obtain a support-free hydrophilized peptide; a support cleavage step B of cleaving a bond between the first peptide chain and the support at any stage before bonding the linker to the supported crude peptide until the hydrophilized peptide is obtained, or after a supported hydrophilized peptide is obtained; a chromatographic purification step of treating the support-free hydrophilized peptide obtained by the hydrophilization step A and the support cleavage step B, by liquid chromatography; and a linker cleavage step of cleaving a bond between the linker and the first peptide chain included in the chromatographically-purified support-free hydrophilized peptide, by chemical treatment.