公开(公告)号：JP2003215141A

公开(公告)日：2003-07-30

申请号：JP2002373067

申请日：2002-12-24

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  APIBIO

Inventor： JAFFARD CLAUDINE ,  BELLEVILLE MARC ,  BOUR CATHERINE

IPC: G01N33/53 ,  B01J19/00 ,  C40B20/02 ,  C40B60/10 ,  C40B70/00 ,  G01N27/327 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide different chips functioned variously on the same wafer, without changing a production process. SOLUTION: In this method for depositing a material selectively on each electrode 21 of a biosensor or chip 20 formed of a single substrate 1, (a) the plurality of biosensors or chips 20, 30, 40, 50, 60, 70 having the plurality of electrodes 21, a plurality of address pads 23 and a plurality of address- recognizable address areas 22 are formed on the same substrate 1, (b) the biosensors or chips are brought selectively into an activated condition by a command word, (c) the material is deposited concurrently on the electrodes by electro-deposition, and (d) the step (b) and the step (c) are repeated with the required frequency required for the material deposition onto the plurality of other electrodes. COPYRIGHT: (C)2003,JPO

公开(公告)号：WO2004081566A2

公开(公告)日：2004-09-23

申请号：PCT/EP2004/002685

申请日：2004-03-15

Applicant: APIBIO SAS ,  MANDRAND, Bernard ,  DUPONT-FILLIARD, Agnès

Inventor： MANDRAND, Bernard ,  DUPONT-FILLIARD, Agnès

IPC: G01N33/53

CPC classification number: B82Y5/00 ,  C12Q1/6825 ,  C12Q1/6837 ,  C12Q2563/125 ,  C12Q2565/113 ,  C12Q2565/607 ,  G01N33/5438 ,  Y10S977/70 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/72

Abstract: The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.

Abstract translation: 本发明涉及用于检测包含多个生物分子的样品中的目标生物分子的方法，由此所述靶生物分子被提供有标签，所述标签包含催化活性部分，其催化产生不溶性反应产物的反应，所述反应产物在 柔性导电纳米电极。 所述纳米电极上的沉淀导致其电导率的变化，其可用于电分析方法。 本发明进一步涉及一种包括固体支持物的生物芯片，其中连接有纳米电极和与所有或多个所述纳米电极结合的探针分子，所述纳米电极可以相同或不同，所述探针分子的片段能够与片段 的目标生物分子。

公开(公告)号：WO03100423A2

公开(公告)日：2003-12-04

申请号：PCT/FR0301574

申请日：2003-05-23

Applicant: APIBIO ,  CUZIN MARC ,  MANDRAND BERNARD ,  CLEUZIAT PHILIPPE ,  ABAIBOU HAFID

Inventor： CUZIN MARC ,  MANDRAND BERNARD ,  CLEUZIAT PHILIPPE ,  ABAIBOU HAFID

IPC: C40B30/04 ,  G01N33/68 ,  G01N33/543

CPC classification number: C40B30/04

Abstract: The invention relates to a biochip (1) comprising a support (2). One useful surface (2a) of the aforementioned support comprises an operating surface (3) having a network of basic sites (Xn) disposed thereon and numerous ligands which are fixed to the respectively different basic sites. The inventive biochip is characterised in that the basic sites are distributed between a central biological analysis area (4) and a peripheral area (5) which defines said central area and which comprises corners that are free of any basic sites.

Abstract translation: 本发明涉及一种包括支撑体（2）的生物芯片（1）。 上述载体的一个有用的表面（2a）包括具有设置在其上的碱性位点（Xn）网络的操作表面（3）和固定到不同基本位点的许多配体。 本发明的生物芯片的特征在于，基本位点分布在中心生物分析区域（4）和限定所述中心区域的周边区域（5）之间，并且包括没有任何基本位置的拐角。

公开(公告)号：WO2005110593A3

公开(公告)日：2006-04-20

申请号：PCT/EP2005005359

申请日：2005-05-17

Applicant: APIBIO SAS ,  LAURENT ALAIN

Inventor： LAURENT ALAIN

IPC: B01J19/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14

CPC classification number: B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00686 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14

Abstract: The present invention relates to the process for the stepwise synthesis of polymers, especially biopolymers, on a solid support surface comprising the steps of providing a solid support surface having a binding site, applying a first microdroplet of a solution comprising a first polymer building block on said binding site to attach the first polymer building block to the binding site and applying to the modified binding site of the preceding step a second microdroplet of a solution comprising the same or a different polymer building block, wherein the solution comprises two different solvents wich have a different viscosity and its relative viscosity (? rel =? second solvent<

Abstract translation: 本发明涉及在固体支持体表面上逐步合成聚合物，特别是生物聚合物的方法，包括以下步骤：提供具有结合位点的固体支持物表面，将包含第一聚合物结构单元的溶液的第一微滴 所述结合位点将第一聚合物结构单元连接到结合位点并向前述步骤的修饰结合位点施加包含相同或不同聚合物结构单元的溶液的第二微滴，其中溶液包含两种不同的溶剂，其具有 不同的粘度及其相对粘度（η3）

公开(公告)号：WO2004076059A3

公开(公告)日：2004-09-10

申请号：PCT/EP2004/002040

申请日：2004-03-01

Applicant: APIBIO SAS ,  IMPIKA SA ,  LAURENT, Alain ,  SARRA-BOURNET, Philippe ,  AUDE, Olivier ,  MORGAVI, Paul ,  MANDRAND, Bernard ,  CUZIN, Marc

Inventor： LAURENT, Alain ,  SARRA-BOURNET, Philippe ,  AUDE, Olivier ,  MORGAVI, Paul ,  MANDRAND, Bernard ,  CUZIN, Marc

IPC: B01J19/00

Abstract: The present invention relates to an automated polymer synthesis apparatus for synthesizing a polymer chain onto a solid substrates by sequentially adding polymer building blocks as well as to a method for synthesizing polymers on solid substrates by sequentially reacting polymer building blocks with reactive groups. The invention further relates to a biochip comprising a solid substrate with reactive groups where biomolecules are attached to and the remaining reactive groups are transformed into chemically inert species.

公开(公告)号：WO03056072A2

公开(公告)日：2003-07-10

申请号：PCT/FR0204555

申请日：2002-12-24

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  APIBIO ,  CAILLAT PATRICE ,  CHARLES RAYMOND ,  GILBERT MARIE LINE ,  REVOL-CAVALIER FREDERIC ,  COLIN BRUNO

Inventor： CAILLAT PATRICE ,  CHARLES RAYMOND ,  GILBERT MARIE LINE ,  REVOL-CAVALIER FREDERIC ,  COLIN BRUNO

IPC: B01J19/00 ,  C25D5/02 ,  C12Q1/00 ,  C23C18/16 ,  G01N27/403 ,  G01N33/543

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C25D5/02 ,  C25D17/06 ,  C25D17/12

Abstract: The invention concerns a method for fixing by electrodeposition reagents on a planar surface (11) of a substrate (10), the planar surface having a plurality of sites (12) designed to be electrically polarized; said method comprises the following steps to be repeated as many times as necessary: depositing on the planar surface (11) a thin electrolyte film (17) containing a chemical species bearing a reagent and designed to be fixed by electrodeposition on at least one site (12); polarizing said site (12) and electrodeposition of said chemical species bearing the reagent on said site; rinsing the planar surface (11).

Abstract translation: 本发明涉及一种通过电沉积试剂固定在基底（10）的平面（11）上的方法，该平面具有设计为电极化的多个部位（12）; 所述方法包括以下步骤：根据需要重复多次：在平坦表面（11）上沉积含有含有试剂的化学物质并设计为通过电沉积固定在至少一个位点上的薄电解质膜（17） 12）; 使所述部位（12）极化并使所述载体上的所述化学物质电沉积在所述部位上; 冲洗平面（11）。

公开(公告)号：DE10308931A1

公开(公告)日：2004-09-23

申请号：DE10308931

申请日：2003-02-28

Applicant: APIBIO SAS GRENOBLE ,  IMPIKA SA AUBAGNE

Inventor： LAURENT ALAIN ,  SARRA-BOURNET PHILIPPE ,  AUDE OLIVIER ,  MORGAVI PAUL ,  BERNARD MANDRAND ,  CUZIN MARC

IPC: B01J19/00 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14 ,  C40B70/00 ,  C07H21/00 ,  C07H1/00

Abstract: The present invention relates to an automated polymer synthesis apparatus for synthesizing a polymer chain onto a solid substrates by sequentially adding polymer building blocks as well as to a method for synthesizing polymers on solid substrates by sequentially reacting polymer building blocks with reactive groups. The invention further relates to a biochip comprising a solid substrate with reactive groups where biomolecules are attached to and the remaining reactive groups are transformed into chemically inert species.

公开(公告)号：AU2003255599A1

公开(公告)日：2003-12-12

申请号：AU2003255599

申请日：2003-05-23

Applicant: APIBIO

Inventor： ABAIBOU HAFID ,  CUZIN MARC ,  MANDRAND BERNARD ,  CLEUZIAT PHILIPPE

IPC: C40B30/04 ,  G01N33/68 ,  G01N33/543

公开(公告)号：FR2840072A1

公开(公告)日：2003-11-28

申请号：FR0207039

申请日：2002-05-24

Applicant: APIBIO

Inventor： CUZIN MARC ,  MANDRAND BERNARD ,  CLEUZIAT PHILIPPE

IPC: C40B30/04 ,  G01N33/68 ,  G01N33/543 ,  B01L3/00 ,  C12Q1/68 ,  G01N33/58

Abstract: Biochip (1) comprises a support (2) the functional side of which has a working surface (3) with a network of elementary sites (Xn), with many ligands, different for each Xn, attached to them. Biochip (1) comprises a support (2) the functional side of which has a working surface (3) with a network of elementary sites (Xn), with many ligands, different for each Xn, attached to them. The new feature is that Xn are distributed between: (i) a central zone (4), designed for detection of at least one target species, with each site containing ligands for a particular target; and (ii) a peripheral zone (5), surrounding (4) and containing control sites, which optionally carry control ligands.

公开(公告)号：FR2834383A1

公开(公告)日：2003-07-04

申请号：FR0116922

申请日：2001-12-27

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  APIBIO

Inventor： JAFFARD CLAUDINE ,  BELLEVILLE MARC ,  BOUR CATHERINE

IPC: B01J19/00 ,  C40B20/02 ,  G01N33/53 ,  C40B60/10 ,  C40B70/00 ,  G01N27/327 ,  G01N37/00 ,  H01L21/768

Abstract: A method for selective deposition of materials (I) on biocollectors or chips (BC/C's) formed on the same substrate. The method involves activation of electrodes, of particular BC/C's on which (I) is to be electrodeposited, using an addressing and command system. Selective deposition of materials (I) on biocollectors or chips (BC/C's) formed on the same substrate involves: (1) forming several BC/C's on the substrate, where: (a) each BC/C has electrodes (on which (I) is deposited), address terminals and an address zone (containing the programmed address of the BC/C, connected to the address terminals and able to recognize the address of the BC/C and activate the BC/C when the exact command is received by the address terminal); and (b) the substrate also includes connectors (specifically repeaters) for linking the address terminals and electrodes of each BC/C to an addressing/activating system; (2) addressing the BC/C's on which (I) is to be deposited by sending suitable commands for successive activation of the BC/C's and connecting the electrodes to receive (I) to polarizing systems by addressing these electrodes; (3) depositing (I) simultaneously on the relevant electrodes by electrodeposition; and (4) repeating steps (2) and (3) as many times as necessary.