公开(公告)号：JP2009216711A

公开(公告)日：2009-09-24

申请号：JP2009115052

申请日：2009-05-11

Applicant: Applied Biosystems Inc ,  アプライド バイオシステムズ インコーポレイテッド

Inventor： SANDELL DONALD

IPC: G01N21/17 ,  B01L7/00 ,  G01N21/25 ,  G01N21/64 ,  G02B3/00 ,  G02B3/08

CPC classification number: G01N21/6452 ,  B01L7/52 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/253 ,  G02B3/0056 ,  G02B3/08

Abstract: PROBLEM TO BE SOLVED: To solve complexity of a lens in an optical detection system for a thermal cycling device. SOLUTION: The optical detection system for the thermal cycling device includes at least one light source 16, a light detection device 12 for detecting light received from a plurality of biological samples, and a lens 20 having a first surface 22 and a second surface 24 formed thereon, the second surface being practically opposed to the first surface. The first surface collimates the light and the second surface leads the light to each of the plurality of biological samples 60. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了解决用于热循环装置的光学检测系统中的透镜的复杂性。 解决方案：用于热循环装置的光学检测系统包括至少一个光源16，用于检测从多个生物样品接收的光的光检测装置12和具有第一表面22和第二表面22的透镜20 形成在其上的表面24，第二表面实际上与第一表面相对。 第一表面准直光，第二表面将光引导到多个生物样品60中的每一个上。版权所有：（C）2009，JPO＆INPIT

公开(公告)号：JP2009143946A

公开(公告)日：2009-07-02

申请号：JP2009026772

申请日：2009-02-06

Applicant: Applied Biosystems Inc ,  アプライド バイオシステムズ インコーポレイテッド

Inventor： KHAN SHAHEER H ,  MENCHEN STEVEN M ,  ROSENBLUM BARNETT B

IPC: C07H19/10 ,  C12N15/09 ,  C07H19/04 ,  C07H21/00 ,  C12Q1/68

CPC classification number: C07H21/00 ,  C07H19/04 ,  C12Q1/6869 ,  Y02P20/55

Abstract: PROBLEM TO BE SOLVED: To provide a labeled polynucleotide. SOLUTION: A substituted propargylethoxyamido nucleoside with a structure represented by figure (I) is disclosed, in which X is selected from the group consisting of an aminoalkanoic acid, an alkylaminobenzoic acid, α-benzoic acid, and 4-amino-2-butynic acid; R 1 and R 2 are each individually selected from the group consisting of -H, a lower alkyl group, a protective group, and a label; R 3 is selected from the group consisting of -H and a lower alkyl group; and B is 7-deazapurine, purine, or a pyrimidine nucleoside base. In addition, a primer extension method is provided, which uses the above X-substituted propargylethoxyamido nucleoside, and a polynucleotide containing the above X-substituted propargylethoxyamido nucleoside is provided. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供标记的多核苷酸。 解决方案：公开了具有由图（I）表示的结构的取代的炔丙氧基酰胺基核苷，其中X选自氨基链烷酸，烷基氨基苯甲酸，α-苯甲酸和4-氨基-2 - 丁酸; R 1 和R SB 2各自分别选自-H，低级烷基，保护基和标记; R SB 3选自-H和低级烷基; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基。 此外，提供了使用上述X取代的炔丙氧基酰氨基核苷的引物延伸方法，并且提供了含有上述X取代的炔丙氧基酰氨基核苷的多核苷酸。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009229468A

公开(公告)日：2009-10-08

申请号：JP2009124765

申请日：2009-05-22

Applicant: Applied Biosystems Inc ,  アプライド バイオシステムズ インコーポレイテッド

Inventor： VANN CHARLES S ,  LAO KAI QIN ,  REED MARK

IPC: G01N33/53 ,  G01N33/543 ,  A61K20060101 ,  C07H21/02 ,  C12N9/22 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N33/566

CPC classification number: C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6827 ,  C12Q2563/155 ,  C12Q2561/125 ,  C12Q2545/114 ,  C12Q2565/102 ,  C12Q2563/143

Abstract: PROBLEM TO BE SOLVED: To provide a method and a composition for detecting a target in a sample. SOLUTION: A method for quantifying the target comprises forming a reaction mixture comprising a sample possibly containing the target, a codable label, one or more target-specific probes each of which binds specifically to the target under selective binding conditions, and a separating portion; treating the reaction mixture under reaction conditions such that a detectable complex is produced when the target is present, wherein the detectable complex includes the codable label, the target-specific probe and the separating portion; and counting the number of codable labels to quantify the target. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供用于检测样品中的靶的方法和组合物。 解决方案：用于定量靶的方法包括形成包含可能含有靶的样品，可编码标记，一种或多种在选择性结合条件下特异性结合靶的靶特异性探针的反应混合物，以及 分离部分 在反应条件下处理反应混合物，使得当靶存在时产生可检测的复合物，其中可检测的复合物包括可编码标记，靶特异性探针和分离部分; 并计算可编码标签的数量以量化目标。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009175157A

公开(公告)日：2009-08-06

申请号：JP2009113092

申请日：2009-05-07

Applicant: Applied Biosystems Inc ,  Inst For Systems Biology ,  Univ Of Washington ,  アプライド バイオシステムズ インコーポレイテッド ,  ザ インスティチュート フォー システムズ バイオロジー ,  ユニヴァーシティ オブ ワシントン

Inventor： AEBERSOLD RUDOLF H ,  ZHOU HUILIN ,  RIST BEATE ,  VELLA GEORGE J ,  PURKAYASTHA SUBHASISH ,  PILLAI SASI

IPC: G01N27/62 ,  G01N33/547 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/68

CPC classification number: G01N33/533 ,  G01N33/532 ,  G01N33/58 ,  G01N33/582 ,  G01N2458/15 ,  Y10T436/24

Abstract: PROBLEM TO BE SOLVED: To provide a method for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups and a reactive group under conditions allowing the sample molecule to covalently bind to the reactive group on the sample molecule, and cleaving the cleavable functional group to release the sample molecule comprising the one or more functional groups (which can be a tag). SOLUTION: The solid support is covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are arranged relative to one another to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供一种通过使样品分子与在包含可切割官能团，一个或多个官能团和反应性基团的化学基团偶联的固体支持物的条件下标记分子的方法，所述条件允许样品 分子与样品分子上的反应性基团共价结合，并切割可切割官能团以释放包含一个或多个官能团（其可以是标签）的样品分子。 解决方案：固体支持物共价偶联到包含可切割官能团，质谱标签和用于共价连接样品分子的反应性基团的化学基团，其中可切割官能团，标签和反应性基团被布置 相对于彼此，以允许在切割可切割官能团时将标签转移到样品分子。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：TW200930818A

公开(公告)日：2009-07-16

申请号：TW097141704

申请日：2008-10-29

Applicant: 應用生命系統股份有限公司 APPLIED BIOSYSTEMS INC.

Inventor： 軒尼詩 洛里 HENNESSY, LORI ,  格林 羅伯特 GREEN, ROBERT

IPC: C12Q ,  G01N

CPC classification number: C12Q1/6876 ,  C12Q2600/156

Abstract: 揭示一些方法及材料以用於在單一多工反應同時放大基因體DNA的至少11種特定STR基因座,做為用於分析此種反應產物的材料及方法。包含於本發明的是在單一多工反應同時放大16種特定基因座的方法及材料,其包含10 AmpFlSTR^ SGMplus^ STR基因座、Amelogenin基因座、及5種新的STR基因座,其包含用於分析這些基因座的方法及材料。

Abstract in simplified Chinese: 揭示一些方法及材料以用于在单一多任务反应同时放大基因体DNA的至少11种特定STR基因座,做为用于分析此种反应产物的材料及方法。包含于本发明的是在单一多任务反应同时放大16种特定基因座的方法及材料,其包含10 AmpFlSTR^ SGMplus^ STR基因座、Amelogenin基因座、及5种新的STR基因座,其包含用于分析这些基因座的方法及材料。

公开(公告)号：DE523982T1

公开(公告)日：1993-07-08

申请号：DE92306490

申请日：1992-07-15

Applicant: APPLIED BIOSYSTEMS, INC., FOSTER CITY, CALIF., US

Inventor： GROSSMAN, PAUL DAVID, BURLINGAME, CA 94010, US

IPC: C07K1/26 ,  G01N27/447

Abstract: A capillary electrophoresis element is disclosed. The elements includes a capillary electrophoresis tube containing a low-voscosity polymer solution having a selected mesh size and low-solution viscosity. The mesh size may range from 50-100 ANGSTROM , for separating single-stranded oligonucleotides, to up to 300 ANGSTROM or greater, for separating relatively large duplex DNA fragments or proteins. Also disclosed is a method for formulating a low-viscosoty electrophoresis separation medium having a selected mesh size.

公开(公告)号：AU632089B2

公开(公告)日：1992-12-17

申请号：AU4916090

申请日：1990-02-06

Applicant: APPLIED BIOSYSTEMS INC.

Inventor： PAU YUAN ,  KUO HSI ,  YEFIM RAYSBERG ,  LARRY KERILA

IPC: G01N21/33 ,  G01N21/64 ,  G01N27/447

公开(公告)号：WO2009079052A2

公开(公告)日：2009-06-25

申请号：PCT/US2008/077092

申请日：2008-09-19

Applicant: APPLIED BIOSYSTEMS INC. ,  YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

Inventor： YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/502707 ,  B01L7/52 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2300/1861 ,  B01L2400/0677

Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.

Abstract translation: 描述了用于隔离化验卡上的室的化验卡和装置以及方法。 测定卡包括由具有软化温度的一种或多种材料（例如塑料）形成的基底，基底限定与相应反应室连通的通道。 可以在通道的区域中将测定卡加热到至少软化温度。 软化的塑料可以变形，例如用可以或不可以提供软化基材的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻塞通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，所述工具装置包括用于加热和使检定卡变形的销。

公开(公告)号：WO2009070871A1

公开(公告)日：2009-06-11

申请号：PCT/CA2008/002083

申请日：2008-11-27

Applicant: MDS ANALYTICAL TECHNOLOGIES, A BUSINESS UNIT OF MDS INC. DOING BUSINESS THROUGH ITS SCIEX DIVISION ,  APPLIED BIOSYSTEMS INC. ,  BONNER, Ron, F. ,  BURTON, Lyle, L. ,  LE BLANC, Yves

Inventor： BONNER, Ron, F. ,  BURTON, Lyle, L. ,  LE BLANC, Yves

IPC: H01J49/26 ,  G01N27/00

CPC classification number: H01J49/004 ,  G01N30/7233 ,  G01N30/86 ,  H01J49/0027 ,  Y10T436/24

Abstract: Systems and methods for analyzing compounds in a sample. In one embodiment, the present technology is directed towards a method of analyzing a sample, comprising: emitting ions from the sample; selecting the emitted ions for a designated ion; fragmenting the designated ions; scanning for a plurality of designated ion fragments; determining a designated fragment chromatographic trace for each designated ion fragment; and generating a combined chromatographic trace corresponding to a non-linear combination of a plurality of designated fragment chromatographic traces.

Abstract translation: 用于分析样品中化合物的系统和方法。 在一个实施例中，本技术涉及分析样品的方法，包括：从样品中发射离子; 选择发射的离子指定的离子; 破碎指定的离子; 扫描多个指定的离子碎片; 确定每个指定离子片段的指定片段色谱痕迹; 以及产生对应于多个指定片段色谱痕迹的非线性组合的组合色谱图。

公开(公告)号：WO2009046348A1

公开(公告)日：2009-04-09

申请号：PCT/US2008/078817

申请日：2008-10-03

Applicant: APPLIED BIOSYSTEMS INC. ,  LEHTO, Dennis A. ,  BOEGE, Steven J.

Inventor： LEHTO, Dennis A. ,  BOEGE, Steven J.

IPC: B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  G01N21/05 ,  G01N35/00

CPC classification number: C12Q1/6874 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/005 ,  B01J2219/00533 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01L3/502 ,  B01L3/50851 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/043 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2300/1811 ,  B01L2300/1894 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/02

Abstract: A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other.

Abstract translation: 用于进行生物样品反应的装置可以包括多个流动池，其构造成安装到普通的显微镜平移台上，其中每个流动池被构造成容纳至少一个含有生物样品的样品架。 每个流动池还可以被配置为选择性地放置在打开位置，用于将至少一个样品保持器定位到流动室中，以及用于使包含在至少一个样品保持器中的生物样品反应的关闭位置。 多个流动池可以被配置为被选择性地彼此相对放置在打开位置和关闭位置。