公开(公告)号：US5830662A

公开(公告)日：1998-11-03

申请号：US619542

申请日：1996-06-21

Applicant: Marcelo B. Soares ,  Argiris Efstratiadis

Inventor： Marcelo B. Soares ,  Argiris Efstratiadis

IPC: C12N15/10 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12N15/1096

Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

Abstract translation: PCT No.PCT / US94 / 10821 Sec。 371日期：1996年6月21日 102（e）日期1996年6月21日PCT 1994年9月23日PCT公布。 公开号WO95 / 08647 1995年3月30日发明内容本发明提供了一种使载体中构建的定向cDNA文库标准化的方法，其允许以单链圆形式繁殖，包括：（a）在单链环中扩增定向cDNA文库; （b）产生与文库中单链圆的3'非编码序列互补的片段以产生部分双链体; （c）纯化部分双链体; （d）将纯化的部分双链体熔化并重新结合至合适的Cot; 和（e）纯化未缔合的单链环，由此产生归一化的cDNA文库。 本发明还提供了通过上述方法产生的标准化cDNA文库和生成的文库的用途。

公开(公告)号：US5637685A

公开(公告)日：1997-06-10

申请号：US465811

申请日：1995-06-06

Applicant: Marcelo B. Soares ,  Argiris Efstratiadis

Inventor： Marcelo B. Soares ,  Argiris Efstratiadis

IPC: C12N15/10 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12N15/1096

Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Abstract translation: 本发明提供了使载体中构建的定向cDNA文库标准化的方法，其允许以单链圆形式繁殖，包括：（a）在单链圆中传播定向cDNA文库; （b）产生与文库中单链圆的3'非编码序列互补的片段以产生部分双链体; （c）纯化部分双链体; （d）将纯化的部分双链体熔化并重新结合至适度的Cot; 和（e）纯化未缔合的单链环，由此产生归一化的cDNA文库。

公开(公告)号：US20210246120A1

公开(公告)日：2021-08-12

申请号：US17271119

申请日：2019-08-23

Applicant: Zoe COURNIA ,  Anna KAPELLA ,  Elias COULADOUROS ,  Argiris EFSTRATIADIS ,  BIOMEDICAL RESEARCH FOUNDATION OF THE ACADEMY OF ATHENS (BRFAA) ,  AGRICULTURAL UNIVERSITY OF ATHENS (AUA) ,  FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS (FORTH)

Inventor： Zoe COURNIA ,  Argiris EFSTRATIADIS ,  Anna KAPELLA ,  Elias COULADOUROS ,  Savvas CHRISTOFORIDIS

IPC: C07D401/14 ,  A61K45/06 ,  C07D213/90

Abstract: 2,6-bis(((1H-benzo[d]imidazol-2-yl)thio)methyl)pyridine and N2, N6-dibenzylpyridine-2, 6-dicarboxamide derivatives and related compounds as phosphoinositide 3-kinase (PI3K) inhibitors for treating cancer. The present invention relates to pharmaceutically active 2,6-bis((1H-benzo[d]imidazol-2-yl)thio)methyl)pyridine, N2,N6-dibenzylpyridine-2, 6-dicarboxamide and N2,N6-bis(3-hydroxyphenyl) pyridine-2, 6-dicarboxamide, as well as to derivatives thereof, and to structurally related compounds. These compounds are phosphoinositide 3-kinase inhibitors (PI3K) and useful in treating or preventing cancerous diseases. The invention further relates methods of manufacturing such compounds as well as to pharmaceutical compositions and formulations comprising such compounds, optionally together with other pharmaceutically active compounds. The invention further relates to a method for determining the activity of PI3Kalpha or PI3Kalpha mutants, which method includes: a) providing a solid phase which is functionalized by immobilization of GST-GRP1-molecules onto the solid phase, b) performing a PI3Kalpha or PI3Kalpha mutant catalyzed enzyme reaction to convert PIP2 to PIP3, c) adding competitor PIP3 carrying a detectable label or reporter molecule, and d) determining enzyme activity based on the amount of PIP3 obtained in step b) which competes with competitor PIP3 for binding to the functionalized solid phase.

公开(公告)号：US20090271879A1

公开(公告)日：2009-10-29

申请号：US12367946

申请日：2009-02-09

Applicant: David B. Berkowitz ,  Sylvain Broussy ,  Argiris Efstratiadis ,  Apostolos Klinakis ,  Matthias Szabolcs

Inventor： David B. Berkowitz ,  Sylvain Broussy ,  Argiris Efstratiadis ,  Apostolos Klinakis ,  Matthias Szabolcs

IPC: A61K49/00 ,  C07D317/70 ,  A61K31/343 ,  C12N9/99 ,  C12N15/74 ,  A01K67/033 ,  A61P35/00

CPC classification number: C07D493/04 ,  A61K31/357 ,  C07D317/70

Abstract: Compounds, compositions, methods of making, and methods of using analogues of (−)-picropodophyllin, as well as a transgenic animal model and its use for identifying anticancer agents.

Abstract translation: 化合物，组合物，制备方法和使用（ - ） - 鬼臼苦素类似物的方法，以及转基因动物模型及其用于鉴定抗癌剂的用途。

公开(公告)号：US5482845A

公开(公告)日：1996-01-09

申请号：US126594

申请日：1993-09-24

Applicant: Marcelo B. Soares ,  Argiris Efstratiadis

Inventor： Marcelo B. Soares ,  Argiris Efstratiadis

IPC: C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12N15/1096

Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Abstract translation: 本发明提供了使载体中构建的定向cDNA文库标准化的方法，其允许以单链圆形式繁殖，包括：（a）在单链圆中传播定向cDNA文库; （b）产生与文库中单链圆的3'非编码序列互补的片段以产生部分双链体; （c）纯化部分双链体; （d）将纯化的部分双链体熔化并重新结合至适度的Cot; 和（e）纯化未缔合的单链环，由此产生归一化的cDNA文库。