Abstract:
A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided.
Abstract:
An optical measurement instrument is an integrated instrument that includes an optical cavity with a light source, a sample cuvette, and an optical sensor. The light source and sensor are on a bench that is on a translational or rotational mechanical platform such that optical beam can be moved to multiple sample containers. The instrument can be used for taking measurements of organism concentration in multiple samples as a production tool for microbiology. Preferably, the instrument holds multiple, individually-loaded, independent fluid samples and determines bacteria concentration via a forward-scattering signal. The instrument can incorporate onboard incubation to promote bacterial growth in the samples during the test. In another aspect, the instrument can be a part of a network for medical diagnostic testing data where data is stored in a manner that is inherently untainted by patient identifiable information.
Abstract:
A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided.
Abstract:
The present invention is a pipette-filtration assembly having a main body defining a first channel, a second channel, a first valve, and a second valve. The first valve is associated with the first channel and permits flow in a first direction while substantially hindering flow in a second direction. The second valve is associated with the second channel and permits flow in the second direction while substantially hindering flow in the first direction. At least one filter is used to filter a fluid sample as the fluid sample flows in the first direction and/or the second direction. The pipette-filtration assembly is preferably disposable and includes one or two stages of integral filtration, primarily for use with an air-displacement pipetting or transfer system.
Abstract:
System and method for detecting and counting bacteria suspended in a biological fluid by means of light scattering measurements is provided. In accordance with the method of the invention the level of signal to noise of the measured intensities of light scattered by a sample of the biological fluid is significantly enhanced for forwardly scattered light within a range of scattering angles which are smaller compared to a predefined maximal scattering angle. The system of the invention includes a cuvette adapted to contain a sample of the biological fluid whose sidewalls and windows are suitably constructed and arranged to significantly reduce the level of reflected light obscuring the scattering patterns measured within the range of scattering angles considered.
Abstract:
Un método para recuento de bacterias suspendidas en un fluido, en que una porción de dicho fluido se filtra para excluir partículas mayores que dichas bacterias y una muestra de la porción filtrada se pone en una cubeta que tiene al menos una ventana iluminada con un haz de luz coherente y colimado, comprendiendo el método las etapas de: i. medir repetidamente a una velocidad de repetición predefinida la intensidad de luz dispersada por la muestra a al menos dos ángulos diferentes de dispersión, ii. calcular representaciones gráficas de diferencia de las mediciones de intensidad de la luz a cada uno de los diferentes ángulos de dispersión a los que se mide la intensidad de la luz, comprendiendo cada representación gráfica de diferencia los valores absolutos de diferencias entre niveles de intensidad de luz medidos en dos momentos de tiempo o diferentes al mismo ángulo de dispersión y iii. igualar cada una de dichas representaciones gráficas de diferencia con una curva de calibración para proporcionar una estimación de la concentración de las bacterias en la muestra.
Abstract:
An instrument determines a concentration of bacteria in a plurality of fluid samples, and comprises a housing, a rotatable platform, a plurality of fluid containers, a light source, a sensor, and a motor. The rotatable platform is within the housing. The fluid containers are located on the rotatable platform. Each fluid container holds a corresponding one of the plurality of fluid samples, and has an input window and an output window. The light source provides an input beam for transmission into the input windows of the fluid containers and through the corresponding fluid samples. The input beam creates a forward-scatter signal associated with the concentration of bacteria. The motor rotates the rotatable platform so that the input beam sequentially passes through each fluid sample. A sensor within the housing detects the forward-scatter signal exiting from the output window associated with the fluid sample receiving the input beam.
Abstract:
System and method for detecting and counting bacteria suspended in a biological fluid by means of light scattering measurements is provided. In accordance with the method of the invention the level of signal to noise of the measured intensities of light scattered by a sample of the biological fluid is significantly enhanced for forwardly scattered light within a range of scattering angles which are smaller compared to a predefined maximal scattering angle. The system of the invention includes a cuvette adapted to contain a sample of the biological fluid whose sidewalls and windows are suitably constructed and arranged to significantly reduce the level of reflected light obscuring the scattering patterns measured within the range of scattering angles considered.
Abstract:
A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided.