公开(公告)号：WO2003060100A3

公开(公告)日：2003-07-24

申请号：PCT/US2003/000820

申请日：2003-01-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  CUMBRE, INC. ,  BERGER, James, M. ,  KECK, James ,  LYNCH, A., Simon ,  CHU, Clement

Inventor： BERGER, James, M. ,  KECK, James ,  LYNCH, A., Simon ,  CHU, Clement

IPC: G01N33/48

Abstract: The present invention provides crystals of a Staphylococcus aureus DnaG primase and Escherichia coli DnaG primase proteins complexed with ligands. The three-dimensional structural information for Staphylococcus aureus DnaG primase with magnesium bound, Staphylococcus aureus DnaG primase with sulfate bound, and Escherichia coli DnaG primase with manganese bound is provided. The present invention provides procedures for identifying agents that can inhibit the cell growth of Staphylococcus aureus, Escherichia coli , or both through the use of rational drug design utilizing the crystallographic data.

公开(公告)号：WO2008154432A3

公开(公告)日：2009-03-26

申请号：PCT/US2008066194

申请日：2008-06-06

Applicant: UNIV CALIFORNIA ,  BERGER JAMES M ,  DONG KEN ,  SCHEOFFLER ALLYN J

Inventor： BERGER JAMES M ,  DONG KEN ,  SCHEOFFLER ALLYN J

IPC: G01N33/483 ,  G06F19/16

CPC classification number: C12N9/90 ,  G06F19/16

Abstract: The present invention provides crystals of a topoisomerase polypeptide and a nucleic acid. The crystals are of use in elucidating the structure of topoisomerase-nucleic acid complexes. Moreover, the crystals are of use in the discovery of ligands that interact with topoisomerase- nucleic acid complexes and modulate the activity of such complexes through this interaction.

Abstract translation: 本发明提供拓扑异构酶多肽和核酸的晶体。 晶体可用于阐明拓扑异构酶 - 核酸复合物的结构。 此外，晶体可用于发现与拓扑异构酶 - 核酸复合物相互作用的配体并通过该相互作用调节这些复合物的活性。

公开(公告)号：WO2004104228A1

公开(公告)日：2004-12-02

申请号：PCT/US2003/031253

申请日：2003-10-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  HANSEN, Carl, L. ,  SKORDALAKES, Emmanuel ,  SOMMER, Morten ,  BERGER, James, M. ,  QUAKE, Stephen, R.

Inventor： HANSEN, Carl, L. ,  SKORDALAKES, Emmanuel ,  SOMMER, Morten ,  BERGER, James, M. ,  QUAKE, Stephen, R.

IPC: C12Q1/68

CPC classification number: C30B29/54 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00756 ,  B01L3/06 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/0472 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0688 ,  C30B7/00 ,  C30B29/58 ,  C40B40/10 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0028 ,  F16K99/0057 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  G01N2013/003

Abstract: The use of microfluidic structures enables high throughput screening of protein crystallization. In one embodiment, an integrated combinatoric mixing chip allows for precise metering of reagents to rapidly create a large number of potential crystallization conditions, with possible crystal formations observed on chip. In an alternative embodiment, the microfluidic structures may be utilized to explore phase space conditions of a particular protein crystallizing agent combination, thereby identifying promising conditions and allowing for subsequent focused attempts to obtain crystal growth.

Abstract translation: 使用微流体结构可实现蛋白质结晶的高通量筛选。 在一个实施方案中，集成的组合混合芯片允许精确计量试剂以快速产生大量潜在的结晶条件，并在芯片上观察到可能的晶体形成。 在替代实施方案中，微流体结构可用于探索特定蛋白质结晶剂组合的相空间条件，从而确定有希望的条件并允许随后的聚焦尝试以获得晶体生长。

公开(公告)号：WO2008154432A2

公开(公告)日：2008-12-18

申请号：PCT/US2008/066194

申请日：2008-06-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERGER, James, M. ,  DONG, Ken ,  SCHEOFFLER, Allyn, J.

Inventor： BERGER, James, M. ,  DONG, Ken ,  SCHEOFFLER, Allyn, J.

IPC: C12Q1/68 ,  G06F19/00

CPC classification number: C12N9/90 ,  G06F19/16

Abstract: The present invention provides crystals of a topoisomerase polypeptide and a nucleic acid. The crystals are of use in elucidating the structure of topoisomerase-nucleic acid complexes. Moreover, the crystals are of use in the discovery of ligands that interact with topoisomerase- nucleic acid complexes and modulate the activity of such complexes through this interaction.

Abstract translation: 本发明提供拓扑异构酶多肽和核酸的晶体。 晶体可用于阐明拓扑异构酶 - 核酸复合物的结构。 此外，晶体可用于发现与拓扑异构酶 - 核酸复合物相互作用的配体并通过该相互作用调节这些复合物的活性。

公开(公告)号：WO2005056813A2

公开(公告)日：2005-06-23

申请号：PCT/US2004/040864

申请日：2004-12-06

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  HANSEN, Carl, L. ,  SOMMER, Morten ,  QUAKE, Stephen, R. ,  BERGER, James, M.

Inventor： HANSEN, Carl, L. ,  SOMMER, Morten ,  QUAKE, Stephen, R. ,  BERGER, James, M.

IPC: C12Q

CPC classification number: C30B29/16 ,  B01F5/10 ,  B01F5/108 ,  B01F13/0059 ,  B01L3/06 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  C30B7/00 ,  C30B7/14 ,  C30B29/58

Abstract: The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.

Abstract translation: 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另外的实施例，井可以被配置为容纳诸如冷冻保护剂的材料，其可用于一旦形成时保护结晶材料。

公开(公告)号：WO2005056813A3

公开(公告)日：2006-08-10

申请号：PCT/US2004040864

申请日：2004-12-06

Applicant: CALIFORNIA INST OF TECHN ,  UNIV CALIFORNIA ,  HANSEN CARL L ,  SOMMER MORTEN ,  QUAKE STEPHEN R ,  BERGER JAMES M

Inventor： HANSEN CARL L ,  SOMMER MORTEN ,  QUAKE STEPHEN R ,  BERGER JAMES M

IPC: C30B35/00 ,  B29C33/40 ,  C12Q20060101 ,  C30B7/00 ,  C30B7/02 ,  C30B29/54

CPC classification number: C30B29/16 ,  B01F5/10 ,  B01F5/108 ,  B01F13/0059 ,  B01L3/06 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  C30B7/00 ,  C30B7/14 ,  C30B29/58

Abstract: The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.

Abstract translation: 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另外的实施例，井可以被配置为容纳诸如冷冻保护剂的材料，其可用于一旦形成时保护结晶材料。

公开(公告)号：WO2003060100A2

公开(公告)日：2003-07-24

申请号：PCT/US2003/000820

申请日：2003-01-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  CUMBRE, INC. ,  BERGER, James, M. ,  KECK, James ,  LYNCH, A., Simon ,  CHU, Clement

Inventor： BERGER, James, M. ,  KECK, James ,  LYNCH, A., Simon ,  CHU, Clement

IPC: C12N

CPC classification number: C12N9/1252 ,  C07K2299/00 ,  C12Q1/18 ,  G01N33/6803

Abstract: The present invention provides crystals of a Staphylococcus aureus DnaG primase and Escherichia coli DnaG primase proteins complexed with ligands. The three-dimensional structural information for Staphylococcus aureus DnaG primase with magnesium bound, Staphylococcus aureus DnaG primase with sulfate bound, and Escherichia coli DnaG primase with manganese bound is provided. The present invention provides procedures for identifying agents that can inhibit the cell growth of Staphylococcus aureus, Escherichia coli , or both through the use of rational drug design utilizing the crystallographic data.

Abstract translation: 本发明提供金属葡萄球菌DnaG primase和与配体络合的大肠杆菌DnaG启动子蛋白的晶体。 提供了具有镁结合的金黄色葡萄球菌DnaG primase，硫酸结合金黄色葡萄球菌DnaG primase和具有锰结合的大肠杆菌DnaG引物酶的三维结构信息。 本发明提供了通过使用结晶学数据使用合理的药物设计来鉴定可以抑制金黄色葡萄球菌，大肠杆菌或两者的细胞生长的试剂的方法。