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    HOMOGENEOUS AND HETEROGENEOUS ASSAY METHODS USING NUCLEATION CENTERS AND NOVEL COVALENT CHEMISTRIES FOR THE RAPID MEASUREMENT OF PHOSPHORYLATION AND DE-PHOSPHORYLATION
    7.
    发明申请
    HOMOGENEOUS AND HETEROGENEOUS ASSAY METHODS USING NUCLEATION CENTERS AND NOVEL COVALENT CHEMISTRIES FOR THE RAPID MEASUREMENT OF PHOSPHORYLATION AND DE-PHOSPHORYLATION 审中-公开
    使用核化中心和新颖的合成化学品进行均相和异质性测定方法，用于快速测定磷化氢和脱磷酸

    公开(公告)号：WO2004089295A2

    公开(公告)日：2004-10-21

    申请号：PCT/US2004/010289

    申请日：2004-04-01

    Applicant: CHROMAGEN, INC. ,  BHATT, Ram, S. ,  MILLER-WING, Allison, V. ,  HELMS, Rebecca ,  GESSERT, Steve ,  GONZALES, Frank ,  CONRAD, Michael, J.

    Inventor： BHATT, Ram, S. ,  MILLER-WING, Allison, V. ,  HELMS, Rebecca ,  GESSERT, Steve ,  GONZALES, Frank ,  CONRAD, Michael, J.

    IPC: A61K

    CPC classification number: C07K17/14 ,  C07K14/00 ,  C12P9/00 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/533 ,  G01N33/542

    Abstract: Novel chemistries, methods and assays are described for the rapid detection and measurement of phosphorylation or de-phosphorylation of polypeptides, oligonucleotides or lipids based upon the use of phosphoryl, phosphoroamino or phosphorothioate specific hetero-bifunctional linkers combined with fluorescence polarization or fluorogenic detection. Homogeneous assay applications possible using these novel chemistries and methods include in vitro fluorescence polarization assays for the detection of general and isoform­ specific polypeptide, oligonucleotide and lipid kinase and phosphatase enzymes. Heterogeneous assay applications possible using the same novel chemistries and assaymethods enable development of in vitro and in vivo fluorogenic assays of general or specific kinase or phosphatase enzyme activities in cell lysates or serum. In all cases, these new chemistries, methods and assays enable simultaneous multi-kinase detection and profiling.

    Abstract translation: 描述了基于使用与荧光偏振或荧光检测结合的磷酰基，磷酰氨基或硫代磷酸酯特异性异双功能连接物快速检测和测量多肽，寡核苷酸或脂质的磷酸化或去磷酸化的新型化学，方法和测定法。 使用这些新颖化学和方法可能的均相测定应用包括用于检测一般和同种型特异性多肽，寡核苷酸和脂质激酶和磷酸酶的体外荧光偏振测定。 使用相同的新颖化学和测定方法可能的非均相测定应用使得能够开发在细胞裂解物或血清中的一般或特异性激酶或磷酸酶活性的体外和体内荧光测定。 在所有情况下，这些新的化学成分，方法和测定可以同时进行多激酶检测和分析。

    DETECTION OR ISOLATION OF TARGET MOLECULES USING A MICROCHANNEL APPARATUS
    9.
    发明申请
    DETECTION OR ISOLATION OF TARGET MOLECULES USING A MICROCHANNEL APPARATUS 审中-公开
    使用MICROCHANNEL设备检测或分离目标分子

    公开(公告)号：WO2008011486A2

    公开(公告)日：2008-01-24

    申请号：PCT/US2007/073817

    申请日：2007-07-18

    Applicant: BIOCEPT, INC. ,  TANG, Zhongliang ,  BHATT, Ram, S. ,  TSINBERG, Pavel

    Inventor： TANG, Zhongliang ,  BHATT, Ram, S. ,  TSINBERG, Pavel

    IPC: C12Q1/68 ,  G01N33/574 ,  C12M1/34

    CPC classification number: G01N1/40 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M47/04 ,  G01N1/405 ,  G01N33/53 ,  G01N33/574

    Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.

    Abstract translation: 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。

    ISOLATION OF CELLS OR THE LIKE FROM BODILY FLUIDS
    10.
    发明申请
    ISOLATION OF CELLS OR THE LIKE FROM BODILY FLUIDS 审中-公开
    细胞分离或类似于体液的分离

    公开(公告)号：WO2006071824A2

    公开(公告)日：2006-07-06

    申请号：PCT/US2005046961

    申请日：2005-12-21

    Applicant: BIOCEPT INC ,  BHATT RAM S ,  TSINBERG PAVEL

    Inventor： BHATT RAM S ,  TSINBERG PAVEL

    IPC: C08G18/10 ,  C08G18/48 ,  G01N33/50

    CPC classification number: A61K9/1641 ,  C12N2533/30

    Abstract: Spheroidal beads present an exterior surface of a hydrophilic permeable, deformable hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents at or near the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound, and the deformable hydrogel allows sequestering agents to retain their native three-dimensional configurations. As a result of such hydrogel characteristics and hydrophilicity, the beads achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.

    Abstract translation: 球形珠呈现亲水性可渗透的可变形水凝胶的外表面，其是通过氨基甲酸酯键聚合并通过氨基甲酸酯和脲键交联的异氰酸酯官能聚合物。 在表面处或附近的螯合剂共价结合到异氰酸酯基团或者如此结合的中间接头上，并且可变形水凝胶允许多价螯合剂保持其天然三维构型。 作为这样的水凝胶特性和亲水性的结果，珠可以很好地捕获来自体液等的非常小的罕见细胞亚群，并且非常有效地阻止了在这种体液中存在的其它生物材料的非特异性结合。 它们可以是全水凝胶球状体或水凝胶涂覆的基底。

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