公开(公告)号：US10197494B2

公开(公告)日：2019-02-05

申请号：US15631618

申请日：2017-06-23

Applicant: BioComp Instruments Inc.

Inventor： David Coombs

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/01 ,  G01N21/64

Abstract: The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

公开(公告)号：US11199490B2

公开(公告)日：2021-12-14

申请号：US16226864

申请日：2018-12-20

Applicant: Biocomp Instruments Inc.

Inventor： David Coombs ,  August Coombs

IPC: G01N21/05 ,  G01N21/17 ,  B01D15/40 ,  G01N21/64 ,  G01N21/31 ,  G01N15/14 ,  B01L3/00 ,  G01N21/03

Abstract: The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

公开(公告)号：US20190128797A1

公开(公告)日：2019-05-02

申请号：US16226864

申请日：2018-12-20

Applicant: Biocomp Instruments Inc.

Inventor： David Coombs ,  August Coombs

IPC: G01N21/05 ,  G01N21/17 ,  B01D15/40 ,  G01N21/64

Abstract: The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

公开(公告)号：US20170370826A1

公开(公告)日：2017-12-28

申请号：US15631618

申请日：2017-06-23

Applicant: BioComp Instruments Inc.

Inventor： David Coombs

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/01

CPC classification number: G01N21/05 ,  G01N21/0303 ,  G01N2021/0193 ,  G01N2021/6482 ,  G01N2201/0627 ,  G01N2201/0806

Abstract: The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

公开(公告)号：EP0461317A1

公开(公告)日：1991-12-18

申请号：EP90306546.4

申请日：1990-06-15

Applicant: BIOCOMP INSTRUMENTS INC.

Inventor： Coombs, David H.

IPC: B01F1/00

CPC classification number: B01F15/0404 ,  B01F9/0014 ,  B01F2009/0063 ,  B01F2215/0037

Abstract: The invention concerns a process and apparatus for forming a continuous concentration gradient in a solution, e.g. of sucrose, in a tube for use in the separation of macromolecules. The process comprises:-

(a) introducing into a tube solutions of differing concentrations in a manner to layer said solutions therein;
(b) inclining said tube to an angle with respect to the vertical;
(c) rotating said tube while maintaining the contents thereof visually determinable thereby to form said continuous solution gradient; and
(d) discontinuing step (c) after formation of said continuous solution gradient.

Preferably the tube is secured in position magnetically.

Abstract translation: 本发明涉及用于在溶液中形成连续浓度梯度的方法和装置，例如， 的蔗糖，用于分离大分子的管中。 该方法包括：（a）将其中的所述溶液层压入不同浓度的管溶液中; （b）使所述管相对于垂直方向倾斜一定角度; （c）旋转所述管，同时保持其目视确定的内容物，从而形成所述连续溶液梯度; 和（d）在形成所述连续溶液梯度后停止步骤（c）。 优选地，管被固定在磁性位置。