公开(公告)号：WO2007090967A2

公开(公告)日：2007-08-16

申请号：PCT/FR2007000236

申请日：2007-02-09

Applicant: BIOMERIEUX SA ,  MALLET FRANCOIS ,  ORIOL GUY ,  CHEYNET VALERIE

Inventor： MALLET FRANCOIS ,  ORIOL GUY ,  CHEYNET VALERIE

IPC: C07K14/15

CPC classification number: C07K14/005 ,  A61K38/162 ,  A61K39/12 ,  A61K39/21 ,  C07K16/1036 ,  C07K2317/34 ,  C12N7/00 ,  C12N2740/10022 ,  C12N2740/10034 ,  C12N2740/10063 ,  G01N2333/15

Abstract: The invention relates to a peptide domain required for interaction between the envelope of a virus pertaining to the HERV-W interference group and an hASCT receptor, comprising an N end point and a C end point. Said peptide domain is defined, at the N end point thereof, by a pattern formed by the amino acids L (Z) a -proline-cysteine-X-cysteine in which Z is any amino acid, a is a whole number between 2 and 30, and X is any amino acid, and at the C end point thereof, by a pattern formed by the amino acids serine-aspartic acid-X a -X b -X c -X d -X e -aspartic acid-X f X g -(Z) ß in which X a , X b , X c , X d , X e , X f X g are any amino acids, Z is any amino acid, ß is a whole number between 15 and 25, preferably 20. The peptide domain comprises, between the N end point and the C end point , at least one pattern selected from the following patterns: a pattern formed by the amino acids cysteine-tyrosine-X 2 -X 3 -X 4 -X 5 - X 6 -cysteine in which X 2 , X 3 , X 4 , X 5 , X 6 are any amino acids, and a pattern formed by the amino acids cysteine-X 7 -X 8 -X 9 -X 10 -X 11 - X 12 - X 13 -X 14 -X 15 -cysteine-trytophane in which X 7 , X 8 , X 9 , X 10 , X 11 , X 12 , X 13 , X 14 , X 15 are any amino acids.

Abstract translation: 本发明涉及与HERV-W干扰组有关的病毒的包膜与hASCT受体之间的相互作用所需的肽结构域，其包含N个终点和C个终点。 所述肽结构域在N末端被由氨基酸L（Z）Λ-脯氨酸 - 半胱氨酸-X-半胱氨酸形成的图案定义，其中Z是任何氨基酸， 是2至30之间的整数，X是任何氨基酸，并且在C末端，由氨基酸丝氨酸 - 天冬氨酸-XA-N _{- - - - - - - - - - - - - - - - - - - - - - - - - - - - - 其中X aα，X B 2，X C 1，N 2，Z 3，...， X，X，X，X，X，X，X是任何氨基酸，Z是任何氨基酸 ，β是15至25之间，优选为20的整数。肽结构域在N端点和C端点之间包含至少一种选自以下模式的图案：由氨基酸半胱氨酸 - 酪氨酸形成的图案 -X 2 -X 3 -X 3 -X 5 -X 6 -X 3 - 半胱氨酸，其中X 2，X 3，X 4，X 5， X，X 6是任何氨基酸，并且由氨基酸半胱氨酸-X7 -X-X ，X 15是任何氨基酸。}

公开(公告)号：WO03044533A2

公开(公告)日：2003-05-30

申请号：PCT/FR0203999

申请日：2002-11-21

Applicant: BIOMERIEUX SA ,  MALLET FRANCOIS ,  DELAIR THIERRY ,  CHEYNET VALERIE ,  ORIOL GUY ,  VERON LAURENT ,  CHARLES MARIE-HELENE ,  ALLARD LAURE

Inventor： MALLET FRANCOIS ,  DELAIR THIERRY ,  CHEYNET VALERIE ,  ORIOL GUY ,  VERON LAURENT ,  CHARLES MARIE-HELENE ,  ALLARD LAURE

IPC: G01N33/543

CPC classification number: G01N33/54393

Abstract: The invention concerns a method for obtaining a capture phase of a target biological material, comprising a modified protein of interest, capable of binding with said target biological material and immobilized on an immobilization phase which consists in providing at least two different peptide sequences, including the peptide sequence of the protein of interest, and a succession of at least six lysine residues and a succession of at least six histidine residues at their N- and C- termini, immobilizing each of the two peptide sequences obtained on the immobilization phase, determining the efficacy of the coupling between each of the two sequences and the immobilization phase, selecting as capture phase, the peptide sequence more efficiently coupled to the immobilization phase. The invention also concerns a method for obtaining a detection phase of a target biological material, comprising a modified protein of interest capable of binding with said target biological material and coupled to a hapten, which consists in providing at least two different peptide sequences, including the peptide sequence of the protein of interest, and a succession of at least six lysine residues and a succession of at least six histidine residues at their N- and C- termini, coupling each of the two peptide sequences obtained to the hapten, determining the efficacy of the coupling between each of the two sequences to the hapten, selecting as detection phase, the peptide sequence more efficiently coupled to the hapten, and the use of the capture and detection phases obtained for detecting a target biological material, in ELISA.

Abstract translation: 本发明涉及一种获得目标生物材料的捕获相的方法，其包含能够与所述靶生物材料结合并且固定在固定相上的修饰的目的蛋白质，所述固定相包含提供至少两种不同的肽序列，其包括 肽序列，以及连续至少六个赖氨酸残基和在其N-和C-末端连续至少六个组氨酸残基，固定在固定化阶段获得的两个肽序列中的每一个，确定 在两个序列中的每一个和固定相之间的偶联的功效，选择作为捕获阶段，肽序列更有效地与固定相结合。 本发明还涉及一种获得目标生物材料的检测阶段的方法，其包含目标生物材料能够与所述靶生物材料结合并与半抗原结合的修饰的目标蛋白质，其包括提供至少两种不同的肽序列，包括 肽序列，以及连续至少六个赖氨酸残基和在其N-和C-末端连续至少六个组氨酸残基，将获得的两个肽序列中的每一个偶联至半抗原，测定功效 的两个序列中的每一个与半抗原之间的偶联，选择作为检测阶段，更有效地偶联到半抗原的肽序列，以及在ELISA中用于检测靶生物材料获得的捕获和检测相的使用。