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公开(公告)号：WO2003025146A2
公开(公告)日：2003-03-27
申请号：PCT/US2002/029878
申请日：2002-09-18
Applicant: AVIGENICS, INC. , RAPP, Jeffrey, C. , CHRISTMANN, Leandro
Inventor： RAPP, Jeffrey, C. , CHRISTMANN, Leandro
IPC: C12N
CPC classification number: A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/20 , C07K2317/23 , C12N15/8509 , C12N2799/027 , C12N2800/30 , C12N2830/008 , C12N2830/15 , C12N2830/46 , C12N2830/90 , C12N2840/203
Abstract: This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.
Abstract translation: 本发明提供了将异源编码序列稳定引入鸟的基因组并表达编码序列以产生所需蛋白质或改变鸟的表型的方法。 本发明提供了通过细胞质显微注射将转基因导入禽胚胎细胞的细胞质的优选方法。 然后，胚胎发育成能够表达异源蛋白质和/或能够通过育种产生一系列转基因鸟的转基因成体。 本发明还提供了可用于该方法的合成载体和基因启动子，以及表达异源蛋白质和含有异源蛋白质的禽卵的转基因鸟类。
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2.发明申请MICROINJECTION ASSEMBLY AND METHODS FOR MICROINJECTING AND REIMPLANTING AVIAN EGGS 审中-公开微量注射组件及微禽注射和重新注射方法
公开(公告)号：WO2002064727A2
公开(公告)日：2002-08-22
申请号：PCT/US2002/003302
申请日：2002-02-06
Applicant: AVIGENICS, INC. , CHRISTMANN, Leandro
Inventor： CHRISTMANN, Leandro
IPC: C12M
CPC classification number: A01K67/0275 , A01K2217/05 , C12M35/00 , G02B21/32
Abstract: The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.
Abstract translation: 本发明提供了一种显微注射组件，其包括显微镜，包括显微操作器，微量移液管和压电振荡器的显微注射系统以及倾斜成角度的宏观监测单元。 本发明还提供微注射禽蛋生发盘的方法，由此将转基因核，精子或分离的核酸递送至禽胚。 可以将禽卵寄回给雌鸟进行硬壳沉积并将卵孵化为转染的鸟。
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3.发明申请
公开(公告)号：WO02064727A3
公开(公告)日：2002-10-31
申请号：PCT/US0203302
申请日：2002-02-06
Applicant: AVIGENICS INC , CHRISTMANN LEANDRO
Inventor： CHRISTMANN LEANDRO
IPC: A01K67/027 , C12M3/00 , A01K45/00 , A61K48/00 , C12N15/00
CPC classification number: A01K67/0275 , A01K2217/05 , C12M35/00 , G02B21/32
Abstract: The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.
Abstract translation: 本发明提供了一种显微注射组件，其包括显微镜，显微注射系统，其包括显微操纵器，微量移液管和压电振荡器，以及倾斜的宏观监测单元。 本发明还提供了对禽卵的生发盘进行显微注射的方法，从而将转基因核，精子或分离的核酸递送给禽类胚胎。 禽卵可以返回到一只雌鸟，用于硬壳沉积，并将鸡蛋孵化成为转染的鸟。
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Patent Agency Ranking
公开(公告)号：WO2006093847A1
公开(公告)日：2006-09-08
申请号：PCT/US2006/006752
申请日：2006-02-24
Applicant: AVIGENICS, INC. , CHRISTMANN, Leandro , EBERHARDT, Dawn, M. , LEAVITT, Markley, C. , HARVEY, Alex, J.
Inventor： CHRISTMANN, Leandro , EBERHARDT, Dawn, M. , LEAVITT, Markley, C. , HARVEY, Alex, J.
CPC classification number: C12N15/8509 , A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/30 , A01K2267/01 , C12N2800/204 , C12N2800/30 , C12N2840/20
Abstract: The invention includes avians containing an artificial chromosome in their genome and methods of making the avians.
Abstract translation: 本发明包括在其基因组中含有人造染色体的鸟类和制作鸟类的方法。
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公开(公告)号：WO2002020752A3
公开(公告)日：2002-03-14
申请号：PCT/US2001/026723
申请日：2001-08-27
Applicant: AVIGENICS, INC. Georgia Bio-Business Center , CHRISTMANN, Leandro , PRATT, Scott, L. , RAPP, Jeffrey, C.
Inventor： CHRISTMANN, Leandro , PRATT, Scott, L. , RAPP, Jeffrey, C.
IPC: C12N15/00
Abstract: Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.
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公开(公告)号：WO0220752A2
公开(公告)日：2002-03-14
申请号：PCT/US0126723
申请日：2001-08-27
Applicant: AVIGENICS INC , CHRISTMANN LEANDRO , PRATT SCOTT L , RAPP JEFFREY C
Inventor： CHRISTMANN LEANDRO , PRATT SCOTT L , RAPP JEFFREY C
CPC classification number: C12N15/8509 , A01K67/0273 , A01K2217/05 , A01K2217/075 , A01K2227/30 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N2770/24222
Abstract: Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.
Abstract translation: 本文描述了通过核转移和通过核转移和卵转移的组合产生克隆的非人动物的方法，其使用受体细胞核的近红外可视化。 提供转基因，敲除和敲门禽鸟。 含有外源蛋白质的卵的生产方法包括在本发明中。 因此，本发明满足了生成克隆鸟类的有效途径的需要。
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公开(公告)号：WO2005084430A1
公开(公告)日：2005-09-15
申请号：PCT/US2005/006072
申请日：2005-02-28
Applicant: AVIGENICS, INC , CHRISTMANN, Leandro , EBERHARDT, Dawn , HARVEY, Alex, J. , LEAVITT, Markley, C.
Inventor： CHRISTMANN, Leandro , EBERHARDT, Dawn , HARVEY, Alex, J. , LEAVITT, Markley, C.
IPC: A01K67/027
CPC classification number: A01K67/0275 , A01K2217/07 , A01K2227/30 , A01K2267/00 , A01K2267/01 , C12N15/8509 , C12N15/907 , C12N2800/90 , C12N2840/203
Abstract: The invention includes transchromosomal avians and transchromosomal avian cells and methods for the introduction of artificial chromosomes into the genome of avians and avian cells.
Abstract translation: 本发明包括转染色体鸟类和转染色体禽类细胞以及将人造染色体引入鸟类和禽类细胞基因组的方法。
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公开(公告)号：WO2003025146A3
公开(公告)日：2003-03-27
申请号：PCT/US2002/029878
申请日：2002-09-18
Applicant: AVIGENICS, INC. , RAPP, Jeffrey, C. , CHRISTMANN, Leandro
Inventor： RAPP, Jeffrey, C. , CHRISTMANN, Leandro
IPC: C12N15/00
Abstract: This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.
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