公开(公告)号：WO2005014640A1

公开(公告)日：2005-02-17

申请号：PCT/GB2004/003312

申请日：2004-07-30

Applicant: CSS-ALBACHEM LIMITED ,  STEWART, Andrew, Smith, Johnstone

Inventor： STEWART, Andrew, Smith, Johnstone

IPC: C07K14/52

CPC classification number: C07K1/042 ,  C07K14/521

Abstract: A method for synthesising a given peptide or its derivative which contains a proline residue or a proline derivative, at proximity to, or at, the C-terminal end of said peptide is provided. The method comprises a) synthesising on a first resin a C­terminal portion of said peptide, o r its derivative, comprising at least three successive amino acid residues or their derivatives, by successive coupling of selected amino acids, small peptides or their derivatives, b) cleaving the C-terminal portion from said first resin; c) reattaching said C-terminal portion to a second resin which is generally suitable for the synthesis of peptides but is unsuitable for the formation of peptides having a proline residue positioned atthe C-terminal end of said peptide; and d)coupling selected amino acids, small peptides or derivatives to the C-terminal portion.

Abstract translation: 提供了一种在所述肽的C-末端合成含有脯氨酸残基或脯氨酸衍生物，邻近或位于其上的给定肽或其衍生物的方法。 该方法包括：a）通过连续偶联选择的氨基酸，小肽或其衍生物，在第一树脂上合成包含至少三个连续的氨基酸残基或其衍生物的所述肽或其衍生物的C末端部分，b）裂解 所述第一树脂的C末端部分; c）将所述C末端部分重新连接到通常适于合成肽的第二树脂，但不适于形成具有位于所述肽的C末端的脯氨酸残基的肽; 和d）将选择的氨基酸，小肽或衍生物偶联到C末端部分。

公开(公告)号：WO2005014620A1

公开(公告)日：2005-02-17

申请号：PCT/GB2004/003391

申请日：2004-08-05

Applicant: CSS-ALBACHEM LIMITED ,  COTTON, Graham

Inventor： COTTON, Graham

IPC: C07K1/107

CPC classification number: C07K1/1077

Abstract: A method of ligating two or more molecules, for example, peptides to peptides or peptides to labels is provided. The method may comprise the steps: a) providing a first oligopeptide having a reactive moiety, which is a hydrazine moiety, a hydrazide moiety or an amino-óxy moiety b) providing a second oligopeptide having an activated ester moiety, c)allowing the reactive moiety of the first oligopeptide to react with the activated ester moiety of the second oligopeptide to form an oligopeptide product, in which the first and second oligopeptides are linked via a linking moiety having Formula I, II or III. The second oligopeptide may preferably be generated by thiol reagent induced cleavage of an intein fusion protein. The invention further provides labelling and ligation methods in which protein hydrazides are ligated by reaction of the hydrazide moiety with an aldehyde functionality or a ketone functionality.

Abstract translation: 提供了将两个或多个分子例如肽与肽或肽连接至标记的方法。 该方法可以包括以下步骤：a）提供具有反应性部分的第一寡肽，其为肼部分，酰肼部分或氨基-o-部分b）提供具有活化酯部分的第二寡肽，c）允许反应性 第一寡肽的部分与第二寡肽的活化酯部分反应以形成寡肽产物，其中第一和第二寡肽通过具有式I，II或III的连接部分连接。 第二寡肽可以优选地由硫醇试剂诱导的内蛋白融合蛋白的切割产生。 本发明进一步提供了标记和连接方法，其中蛋白质酰肼通过酰肼部分与醛官能团或酮官能团的反应连接。

公开(公告)号：WO2004106363A2

公开(公告)日：2004-12-09

申请号：PCT/GB2004/002327

申请日：2004-06-01

Applicant: CSS-ALBACHEM LIMITED ,  HAY, Alastair ,  COTTON, Graham ,  RAMAGE, Robert

Inventor： HAY, Alastair ,  COTTON, Graham ,  RAMAGE, Robert

IPC: C07K1/13

CPC classification number: C07K1/13 ,  C07D213/38

Abstract: Disclosed is a tag for purification of peptides, The tag structure facilitates the easy cleavage of a bond formed between the tag molecule and a peptide to which it is bound under conditions which minimise, indeed preferably prevent, damage to the peptide. The tag molecules of the invention may be used to separate and/or purify a peptide from a mixture of peptides and/or other components.

Abstract translation: 披露了用于纯化肽的标签。标签结构有助于在最小化，实际上优选地防止对肽的损伤的条件下容易地切割标签分子与其所结合的肽之间形成的键。 本发明的标签分子可用于从肽和/或其它组分的混合物分离和/或纯化肽。

公开(公告)号：EP1651664A1

公开(公告)日：2006-05-03

申请号：EP04767986.5

申请日：2004-08-05

Applicant: CSS-Albachem Limited

Inventor： COTTON, Graham

IPC: C07K1/107 ,  C07K19/00

CPC classification number: C07K1/1077

Abstract: A method of ligating two or more molecules, for example, peptides to peptides or peptides to labels is provided. The method may comprise the steps: a) providing a first oligopeptide having a reactive moiety, which is a hydrazine moiety, a hydrazide moiety or an amino-óxy moiety b) providing a second oligopeptide having an activated ester moiety, c)allowing the reactive moiety of the first oligopeptide to react with the activated ester moiety of the second oligopeptide to form an oligopeptide product, in which the first and second oligopeptides are linked via a linking moiety having Formula I, II or III. The second oligopeptide may preferably be generated by thiol reagent induced cleavage of an intein fusion protein. The invention further provides labelling and ligation methods in which protein hydrazides are ligated by reaction of the hydrazide moiety with an aldehyde functionality or a ketone functionality.

公开(公告)号：EP1648930A1

公开(公告)日：2006-04-26

申请号：EP04767950.1

申请日：2004-07-30

Applicant: CSS-Albachem Limited

Inventor： STEWART, Andrew Smith Johnstone

IPC: C07K14/52 ,  C07K1/04

CPC classification number: C07K1/042 ,  C07K14/521

Abstract: A method for synthesising a given peptide or its derivative which contains a proline residue or a proline derivative, at proximity to, or at, the C-terminal end of said peptide is provided. The method comprises a) synthesising on a first resin a C­terminal portion of said peptide, o r its derivative, comprising at least three successive amino acid residues or their derivatives, by successive coupling of selected amino acids, small peptides or their derivatives, b) cleaving the C-terminal portion from said first resin; c) reattaching said C-terminal portion to a second resin which is generally suitable for the synthesis of peptides but is unsuitable for the formation of peptides having a proline residue positioned atthe C-terminal end of said peptide; and d)coupling selected amino acids, small peptides or derivatives to the C-terminal portion.

公开(公告)号：EP1628998A2

公开(公告)日：2006-03-01

申请号：EP04735606.8

申请日：2004-06-01

Applicant: CSS-Albachem Limited

Inventor： HAY, Alastair ,  COTTON, Graham ,  RAMAGE, Robert

IPC: C07K1/13 ,  C07K1/16

CPC classification number: C07K1/13 ,  C07D213/38

Abstract: Disclosed is a tag for purification of peptides, The tag structure facilitates the easy cleavage of a bond formed between the tag molecule and a peptide to which it is bound under conditions which minimise, indeed preferably prevent, damage to the peptide. The tag molecules of the invention may be used to separate and/or purify a peptide from a mixture of peptides and/or other components.