公开(公告)号：WO2019183924A1

公开(公告)日：2019-10-03

申请号：PCT/CN2018/081338

申请日：2018-03-30

Applicant: SYZ CELL THERAPY CO. ,  HRYZ BIOTECH CO.

Inventor： ZHOU, Xiangjun ,  MA, Yifan ,  HAN, Yanyan ,  LI, Jin ,  TANG, Longqing ,  LIU, Junyun ,  WU, Dongyun

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0786 ,  A61K38/17 ,  A61P35/00

Abstract: Provided are methods of preparing a population activated T cells by co-culturing T cells with dendritic cells loaded with a plurality of tumor antigen peptides. Also provided are methods of treating cancer in an individual using the activated T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.

公开(公告)号：WO2019196088A1

公开(公告)日：2019-10-17

申请号：PCT/CN2018/082947

申请日：2018-04-13

Applicant: SYZ CELL THERAPY CO. ,  HRYZ BIOTECH CO.

Inventor： ZHOU, Xiangjun ,  HAN, Yanyan ,  LIANG, Xiaoling ,  CHEN, Xihe

IPC: C07K14/725 ,  C07K14/705 ,  C07K16/30 ,  C12N15/09 ,  C12N15/10 ,  C12N15/12 ,  A61K39/00 ,  A61K38/00

Abstract: The present application provides methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells are also provided.

公开(公告)号：WO2019196087A1

公开(公告)日：2019-10-17

申请号：PCT/CN2018/082945

申请日：2018-04-13

Applicant: SYZ CELL THERAPY CO. ,  HRYZ BIOTECH CO.

Inventor： ZHOU, Xiangjun ,  HAN, Yanyan ,  CHEN, Xihe ,  LIANG, Xiaoling

IPC: A61P35/00 ,  C12N5/0784 ,  C12N5/078 ,  C12N5/0783 ,  A61K39/385

Abstract: Provided are methods of preparing tumor antigen-specific T cells comprising enriching activated T cells from a first co-culture comprising a first population of antigen-loaded dendritic cells and a population of T cells, and co-culturing the enriched activated T cells with a second population of antigen-loaded dendritic cells. Also provided are methods of treating cancer in an individual using the tumor antigen-specific T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.

公开(公告)号：WO2019185041A1

公开(公告)日：2019-10-03

申请号：PCT/CN2019/080535

申请日：2019-03-29

Applicant: SYZ CELL THERAPY CO. ,  HRYZ BIOTECH CO.

Inventor： ZHOU, Xiangjun ,  MA, Yifan ,  HAN, Yanyan ,  LI, Jin ,  TANG, Longqing ,  LIU, Junyun ,  WU, Dongyun

IPC: C12N5/0783 ,  C12N5/0784 ,  A61K35/17 ,  A61K38/17 ,  A61P35/00

Abstract: The present invention provides methods of preparing a population of activated T cells by co-culturing T cells with dendritic cells loaded with a plurality of tumor antigen peptides. Also provided are methods of treating cancer in an individual using the activated T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.

公开(公告)号：US20230023834A1

公开(公告)日：2023-01-26

申请号：US17843912

申请日：2022-06-17

Applicant: SYZ Cell Therapy Co. ,  HRYZ Biotech Co.

Inventor： Xiangjun ZHOU ,  Yanyan HAN ,  Xihe CHEN

IPC: C07K14/725 ,  A61K39/00 ,  C12N5/0784 ,  C12N5/0783 ,  A61P35/00 ,  C12P21/02 ,  A61K38/00

Abstract: Provided methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Also provided tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells.

公开(公告)号：US20230212253A1

公开(公告)日：2023-07-06

申请号：US17843920

申请日：2022-06-17

Applicant: SYZ Cell Therapy Co. ,  HRYZ Biotech Co.

Inventor： Xiangjun ZHOU ,  Yanyan HAN ,  Xihe CHEN

IPC: C07K14/725 ,  A61P35/00 ,  A61K39/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12P21/02

CPC classification number: C07K14/7051 ,  A61K39/0011 ,  A61P35/00 ,  C12N5/0636 ,  C12N5/0639 ,  C12P21/02 ,  A61K38/00

Abstract: Provided methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Also provided tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells.

公开(公告)号：US20170296639A1

公开(公告)日：2017-10-19

申请号：US15312976

申请日：2015-05-20

Applicant: Shenzhen Institutes of Advanced Technology ,  Hryz Biotech Co.

Inventor： Yifan MA ,  Xiangjun ZHOU ,  Shang CHEN ,  Lintao CAI ,  Ce WANG ,  Peng LIU

IPC: A61K39/00 ,  C12N7/00 ,  A61K39/29 ,  C12N5/0784

CPC classification number: A61K39/0011 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/5154 ,  A61K2039/55555 ,  C12N5/0639 ,  C12N7/00 ,  C12N2500/90 ,  C12N2501/05 ,  C12N2501/22 ,  C12N2501/2304 ,  C12N2730/10134

Abstract: The present invention provides a method for preparing dendritic cell loaded with antigen, the method comprising the steps of adding serum-free cell culture medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and inter-leukin (IL)-4 into mononuclear cells, culturing in an incubator at 37° C. under 5% carbon dioxide for 5 days, adding target antigen wrapped cationic liposome and culturing for 8-24 hours to obtain target antigen loaded dendritic cell.

公开(公告)号：US20220403000A1

公开(公告)日：2022-12-22

申请号：US17843917

申请日：2022-06-17

Applicant: SYZ Cell Therapy Co. ,  HRYZ Biotech Co.

Inventor： Xiangjun ZHOU ,  Yanyan HAN ,  Xihe CHEN

IPC: C07K14/725 ,  A61P35/00 ,  A61K39/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12P21/02

Abstract: Provided methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Also provided tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells.

公开(公告)号：US20220402999A1

公开(公告)日：2022-12-22

申请号：US17843916

申请日：2022-06-17

Applicant: SYZ Cell Therapy Co. ,  HRYZ Biotech Co.

Inventor： Xiangjun ZHOU ,  Yanyan HAN ,  Xihe CHEN

IPC: C07K14/725 ,  A61P35/00 ,  A61K39/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12P21/02

Abstract: Provided methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Also provided tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells.

公开(公告)号：US20220235324A1

公开(公告)日：2022-07-28

申请号：US17668746

申请日：2022-02-10

Applicant: SYZ Cell Therapy Co. ,  HRYZ Biotech Co.

Inventor： Xiangjun ZHOU ,  Yifan MA ,  Yanyan HAN ,  Jin LI ,  Longqing TANG ,  Junyun LIU ,  Dongyun WU

IPC: C12N5/0783 ,  A61P35/00 ,  A61K35/17

Abstract: The present invention provides methods of preparing a population of activated T cells by co-culturing T cells with dendritic cells loaded with a plurality of tumor antigen peptides. Also provided are methods of treating cancer in an individual using the activated T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.