公开(公告)号：EP3265552A2

公开(公告)日：2018-01-10

申请号：EP16800433.1

申请日：2016-03-04

Applicant: Levner, Daniel ,  Hinojosa, Christopher David ,  Van der Meer, Andries ,  Van der Helm, Marinke ,  Jain, Abhishek ,  Ingber, Donald E. ,  Zamani, Marjon

Inventor： Levner, Daniel ,  Hinojosa, Christopher David ,  Van der Meer, Andries ,  Van der Helm, Marinke ,  Jain, Abhishek ,  Ingber, Donald E. ,  Zamani, Marjon

IPC: C12M1/34 ,  C12M1/00 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50

CPC classification number: G01N33/4833 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/084 ,  B01L2400/086 ,  G01N15/0826 ,  G01N33/50 ,  G01N33/5005 ,  G01N2015/0065

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：CA2955172C

公开(公告)日：2021-07-20

申请号：CA2955172

申请日：2015-07-10

Applicant: PRESIDENT AND FELLOWS OF HAVARD COLLEGE ,  HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  LEVNER DANIEL ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ ALCON JOSE ,  INGBER DONALD E

Inventor： HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  LEVNER DANIEL ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ-ALCON JOSE ,  INGBER DONALD E

IPC: B81B7/00 ,  B81B1/00 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/02

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：GB201716183D0

公开(公告)日：2017-11-15

申请号：GB201716183

申请日：2016-03-04

Applicant: LEVNER DANIEL ,  HINOJOSA CHRISTOPHER DAVID ,  MEER ANDRIES ,  HELM MARINKE ,  JAIN ABHISHEK ,  INGBER DONALD E ,  ZAMANI MARJON

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：AU2016266584A1

公开(公告)日：2017-10-26

申请号：AU2016266584

申请日：2016-03-04

Applicant: HINOJOSA CHRISTOPHER DAVID ,  INGBER DONALD E ,  JAIN ABHISHEK ,  LEVNER DANIEL ,  VAN DER HELM MARINKE ,  VAN DER MEER ANDRIES ,  ZAMANI MARJON

Inventor： LEVNER DANIEL ,  HINOJOSA CHRISTOPHER DAVID ,  VAN DER MEER ANDRIES ,  VAN DER HELM MARINKE ,  JAIN ABHISHEK ,  INGBER DONALD E ,  ZAMANI MARJON

IPC: C12M1/34 ,  C12M1/00 ,  C12Q1/00 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：CA2978727A1

公开(公告)日：2016-12-01

申请号：CA2978727

申请日：2016-03-04

Applicant: LEVNER DANIEL ,  HINOJOSA CHRISTOPHER DAVID ,  VAN DER MEER ANDRIES ,  VAN DER HELM MARINKE ,  JAIN ABHISHEK ,  INGBER DONALD E ,  ZAMANI MARJON

Inventor： LEVNER DANIEL ,  HINOJOSA CHRISTOPHER DAVID ,  VAN DER MEER ANDRIES ,  VAN DER HELM MARINKE ,  JAIN ABHISHEK ,  INGBER DONALD E ,  ZAMANI MARJON

IPC: C12Q1/00 ,  C12M1/00 ,  C12M1/34 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：CA2955172A1

公开(公告)日：2016-01-21

申请号：CA2955172

申请日：2015-07-10

Applicant: PRESIDENT AND FELLOWS OF HAVARD COLLEGE ,  HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  LEVNER DANIEL ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ-ALCON JOSE ,  INGBER DONALD E

Inventor： HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  LEVNER DANIEL ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ-ALCON JOSE ,  INGBER DONALD E

IPC: B81B7/00 ,  B81B1/00 ,  C12M1/00 ,  C12Q1/02

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：WO2017024044A1

公开(公告)日：2017-02-09

申请号：PCT/US2016/045369

申请日：2016-08-03

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  INGBER, Donald E. ,  HENRY, Olivier Y.F. ,  SUPER, Michael

Inventor： INGBER, Donald E. ,  HENRY, Olivier Y.F. ,  SUPER, Michael

IPC: B01J14/00 ,  C12Q1/68 ,  G01F1/64 ,  G01N27/416 ,  G01N33/50

CPC classification number: G01N33/581 ,  B01J2219/00653 ,  B82Y30/00 ,  C12Q1/68 ,  C12Q1/6816 ,  G01F1/64 ,  G01N33/5438 ,  C12Q2565/607

Abstract: The invention described herein relates generally to methods, sensors, devices and kits for electrochemical detection of a target analyte in a sample. In certain aspects, the methods, sensors, devices and kits described herein can be used to detect low concentrations of at least one target analyte using small sample volumes. In some embodiments, methods, sensors and kits for detecting a microbe, microbe fragment or released endotoxin in a test sample, including bodily fluids such as blood and tissues of a subject, food, water, and environmental surfaces, are also provided herein.

Abstract translation: 本文描述的发明一般涉及用于电化学检测样品中目标分析物的方法，传感器，装置和试剂盒。 在某些方面，本文描述的方法，传感器，装置和试剂盒可用于使用小样品体积检测至少一种靶分析物的低浓度。 在一些实施例中，本文还提供了用于检测测试样品中的微生物，微生物碎片或释放的内毒素的方法，传感器和试剂盒，包括体液如受试者的血液和组织，食物，水和环境表面。

公开(公告)号：WO2011091037A2

公开(公告)日：2011-07-28

申请号：PCT/US2011/021718

申请日：2011-01-19

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  YUNG, Chong Wing ,  INGBER, Donald E. ,  COOPER, Ryan Mcomber ,  VOLLMER, Frank ,  DOMANSKY, Karel ,  LESLIE, Daniel Christopher ,  SUPER, Michael

Inventor： YUNG, Chong Wing ,  INGBER, Donald E. ,  COOPER, Ryan Mcomber ,  VOLLMER, Frank ,  DOMANSKY, Karel ,  LESLIE, Daniel Christopher ,  SUPER, Michael

IPC: G01N35/08 ,  G01N33/48 ,  G01N33/52 ,  G01N33/58 ,  G01N1/38

CPC classification number: C12Q1/04 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/043 ,  G01N33/54306 ,  G01N33/54333 ,  G01N33/54366 ,  G01N35/0098

Abstract: A microfluidic device of a diagnostic and detection system includes an inlet port connected by one or more microchannels to an outlet port and includes a capture and visualization chamber (CVC) connected to at least one microchannel. A fluid to be analyzed can be mixed with magnetic microbeads that have an affinity to become bound to target components, such as pathogens in the fluid. The fluid including the magnetically bound target components can be injected through the microfluidic device. Magnetic field gradient, such as provided by permanent or elecrto-magnets, can be applied to the fluid and the magnetically bound target components flowing through the microfluidic device to cause the magnetically bound target components to migrate into the (CVC) and become separated from the fluid. The magnetically bound target components can be analyzed and tested using various techniques to detect the presence of specific organic and inorganic materials, such as pathogens in bio-fluids and contamination in liquid food sources (e.g. water). The device and method provide a system for rapidly detecting pathogens and contamination in relatively small fluid samples.

Abstract translation: 诊断和检测系统的微流体装置包括通过一个或多个微通道连接到出口端口的入口端口，并且包括连接到至少一个微通道的捕获和可视化腔室（CVC）。 待分析的流体可以与具有亲和力的磁性微珠混合，以与目标组分（例如流体中的病原体）结合。 包括磁性结合的目标组分的流体可以通过微流体装置注入。 诸如由永久磁体或电磁体提供的磁场梯度可以施加到流体和流过微流体装置的磁性结合的目标组分，以使磁性结合的目标组分迁移到（CVC）中并与 流体。 可以使用各种技术来分析和测试磁性结合的目标组分，以检测特定有机和无机材料（例如生物流体中的病原体和液体食物源（例如水）中的污染）的存在。 该装置和方法提供了用于快速检测相对较小的流体样品中的病原体和污染物的系统。

公开(公告)号：WO2010123545A3

公开(公告)日：2010-10-28

申请号：PCT/US2010/001121

申请日：2010-04-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  CHILDREN'S MEDICAL CENTER CORPORATION ,  COREY, Elias, James ,  CZAKÓ, Barbara ,  KÜRTI, László ,  MAMMOTO, Akiko ,  INGBER, Donald, E.

Inventor： COREY, Elias, James ,  CZAKÓ, Barbara ,  KÜRTI, László ,  MAMMOTO, Akiko ,  INGBER, Donald, E.

IPC: C07J41/00 ,  C07J43/00 ,  A61K31/56 ,  A61P35/00

Abstract: Compounds of Structural Formula I or pharmaceutically acceptable salts thereof, are effective inhibitors of angiogenesis:

公开(公告)号：WO2009149239A1

公开(公告)日：2009-12-10

申请号：PCT/US2009/046219

申请日：2009-06-04

Applicant: CHILDREN'S MEDICAL CENTER CORPORATION ,  INGBER, Donald, E. ,  THODETI, Charles, K.

Inventor： INGBER, Donald, E. ,  THODETI, Charles, K.

IPC: A61K38/00 ,  A61K31/70

CPC classification number: C12N15/1138 ,  A61K31/70 ,  A61K31/713 ,  A61K45/06 ,  C07K16/2842 ,  C12N2310/14 ,  G01N33/5064

Abstract: The present invention relates to methods of inhibiting capillary endothelial (CE) cell migration, the formation of CE networks and angiogenesis, and uses thereof for the purpose of treating angiogenesis-related diseases and disorders, particularly when the diseases or disorders are directly related aberrant angiogenesis. Inhibition is achieved by inhibiting TRPV4 activity, such as the levels of TRPV4 expression, calcium influx through TRPV4, and/or the intracellular signaling from TRPV4 via β1 integrin activation.

Abstract translation: 本发明涉及抑制毛细血管内皮细胞迁移，CE网络形成和血管生成的方法及其用于治疗血管生成相关疾病和病症的用途，特别是当疾病或病症直接相关异常血管生成 。 通过抑制TRPV4活性，例如TRPV4表达的水平，通过TRPV4的钙流入和/或通过β1整联蛋白激活来自TRPV4的细胞内信号传导来实现抑制。