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    COLD-ACTIVE PROTEASE CP70
    1.
    发明申请
    COLD-ACTIVE PROTEASE CP70 审中-公开
    冷活性蛋白CP70

    公开(公告)号：WO1997027313A1

    公开(公告)日：1997-07-31

    申请号：PCT/US1997001148

    申请日：1997-01-24

    Applicant: THE PROCTER & GAMBLE COMPANY ,  JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HASAN, A., K., M., Quamrul ,  TAMIYA, Eiichi

    Inventor： THE PROCTER & GAMBLE COMPANY ,  JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    IPC: C12P21/04

    CPC classification number: A23J3/341 ,  C12N9/52

    Abstract: A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable at a pH in the range of 6.5 to 10.0 at 30 DEG C for 1 hour; (d) optimal temperature: about 40 DEG C; (e) temperature stability: at pH 7 for 1 hour, the protease is scarcely inactivated at a temperature up to 30 DEG C, but it is inactivated at 40 DEG C as much as about 40 % and completely inactivated at 50 DEG C in about 10 minutes; (f) enzyme activity: the protease has about 50 % or more of its maximum activity at 20 DEG C; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.

    Abstract translation: 这里公开了一种具有以下物理化学性质的嗜酸性蛋白酶：（a）比活性和底物特异性：蛋白酶作用于酪蛋白，明胶，血红蛋白和白蛋白，以酪蛋白，明胶，血红蛋白和白蛋白的顺序进行特异性分解; （b）最佳pH：8.0; （c）pH稳定性：蛋白酶在30℃下在6.5至10.0的pH范围内稳定1小时; （d）最适温度：约40℃; （e）温度稳定性：在pH7下1小时，蛋白酶在高达30℃的温度下几乎不失活，但在40℃灭活多达约40％，在约50℃完全灭活 10分钟; （f）酶活性：蛋白酶在20℃下具有约50％以上的最大活性; （g）酶的活性中心是丝氨酸; 和（h）通过SDS-PAGE测量，蛋白酶的分子量为约70kDa。

    COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA
    2.
    发明申请
    COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA 审中-公开
    冷活性蛋白酶CP-58和精神分裂症细菌

    公开(公告)号：WO1997030172A1

    公开(公告)日：1997-08-21

    申请号：PCT/US1997002437

    申请日：1997-02-14

    Applicant: THE PROCTER & GAMBLE COMPANY ,  JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HASAN, A., K., M., Quamrul ,  TAMIYA, Eiichi

    Inventor： THE PROCTER & GAMBLE COMPANY ,  JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    IPC: C12P21/04

    CPC classification number: C12Y304/00 ,  C11D3/386 ,  C12N9/52

    Abstract: A cold-active protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, albumin and hemoglobin to specifically decompose them in the order of casein, gelatin, albumin and hemoglobin; (b) optimal pH: 7.5 to 8.0; (c) pH stability: the protease is stable at a pH in the range of 5.5 to 10.5 at 20 DEG C for 1 hour; (d) optimal temperature: 20 DEG C at pH 10.5 and 40 DEG C at pH 8.0; (e) temperature stability: at pH 10.5 for 1 hour, the protease is scarcely inactivated at a temperature of 10 DEG C to 30 DEG C, but it is inactivated at 40 DEG C as much as about 30 % and completely inactivated at 50 DEG C; (f) enzyme activity: the protease has about 60 % or more of its maximum activity at 20 DEG C; (g) the active center of the enzyme is a metallic ion; and (h) the molecular weight of the protease is about 58 kDa as measured by SDS-PAGE.

    Abstract translation: 这里公开了一种具有以下物理化学性质的冷活性蛋白酶：（a）比活性和底物特异性：蛋白酶作用于酪蛋白，明胶，白蛋白和血红蛋白，以酪蛋白，明胶，白蛋白和血红蛋白的顺序特异性地分解它们 ; （b）最佳pH：7.5至8.0; （c）pH稳定性：蛋白酶在20℃下在5.5至10.5范围内的pH下稳定1小时; （d）最适温度：20℃，pH10.5和40℃，pH8.0; （e）温度稳定性：在pH 10.5下1小时，蛋白酶在10℃至30℃的温度下几乎不失活，但在40℃灭活多达约30％，在50℃完全失活 C; （f）酶活性：蛋白酶在20℃下具有约60％以上的最大活性; （g）酶的活性中心是金属离子; 和（h）通过SDS-PAGE测量，蛋白酶的分子量为约58kDa。

    VITREOUS STATE STABILIZING AGENT FOR ANIMAL CELL CRYOPRESERVATION SOLUTION
    9.
    发明申请
    VITREOUS STATE STABILIZING AGENT FOR ANIMAL CELL CRYOPRESERVATION SOLUTION 有权

    公开(公告)号：US20210084891A1

    公开(公告)日：2021-03-25

    申请号：US16077801

    申请日：2017-02-15

    Applicant: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Kazuaki Matsumura ,  Toshiaki Naka

    IPC: A01N1/02

    Abstract: Provided are a vitrification stabilizer for an animal cell cryopreservation fluid, and an animal cell cryopreservation fluid which exhibits superior vitrification capabilities due to the animal cell cryopreservation fluid containing the vitrification stabilizer for an animal cell cryopreservation fluid. The vitrification stabilizer for an animal cell cryopreservation fluid contains: an amphoteric polymer compound selected from the group consisting of (a) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with butyl succinic anhydride, (b) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with butyl succinic anhydride and succinic anhydride, or (c) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with a compound represented by formula I; and (d) a sucrose polymer macromolecule to which epichlorohydrin has been crosslinked.

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