公开(公告)号：WO2005074541A3

公开(公告)日：2005-10-06

申请号：PCT/US2005002698

申请日：2005-01-31

Applicant: QTL BIOSYSTEMS LLC ,  WANDERS BART J ,  KUSHON STUART A

Inventor： WANDERS BART J ,  KUSHON STUART A

IPC: C12Q1/70 ,  G01N1/00 ,  G01N21/64 ,  G01N33/53 ,  G01N35/00

CPC classification number: G01N21/6428 ,  G01N35/0098 ,  G01N2021/6432

Abstract: Techniques for the sensitive detection of analytes which combine the benefits of solution/suspension phase assay formats and the simplicity of solid phase/lateral flow assays are described. The assays can be performed in the solution/suspension phase using magnetic microspheres as a solid support. Subsequently a magnetic separation can be performed to separate the bound analyte from the remainder of the solution. After a wash step, the fluorescence signal can be directly read from the magnetic particle surface. Portable biodetection systems which employ fluorescent polymer superquenching and methods for detecting bioagents therewith are also described.

Abstract translation: 描述了结合溶液/悬浮相测定形式的优点和固相/侧流测定的简单性的分析物的敏感检测技术。 可以使用磁性微球作为固体支持物在溶液/悬浮相中进行测定。 随后，可以进行磁性分离以将结合的分析物与溶液的其余部分分离。 洗涤步骤后，荧光信号可直接从磁性颗粒表面读取。 还描述了采用荧光聚合物超强化的便携式生物检测系统和用于检测其生物代谢的方法。

公开(公告)号：WO2005074541A2

公开(公告)日：2005-08-18

申请号：PCT/US2005/002698

申请日：2005-01-31

Applicant: QTL BIOSYSTEMS LLC ,  WANDERS, Bart, J. ,  KUSHON, Stuart, A.

Inventor： WANDERS, Bart, J. ,  KUSHON, Stuart, A.

IPC: C12Q1/70 ,  G01N1/00 ,  G01N21/64 ,  G01N33/53 ,  G01N35/00

CPC classification number: G01N21/6428 ,  G01N35/0098 ,  G01N2021/6432

Abstract: Techniques for the sensitive detection of analytes which combine the benefits of solution/suspension phase assay formats and the simplicity of solid phase/lateral flow assays are described. The assays can be performed in the solution/suspension phase using magnetic microspheres as a solid support. Subsequently a magnetic separation can be performed to separate the bound analyte from the remainder of the solution. After a wash step, the fluorescence signal can be directly read from the magnetic particle surface. Portable biodetection systems which employ fluorescent polymer superquenching and methods for detecting bioagents therewith are also described.

Abstract translation: 描述了结合溶液/悬浮相测定形式的优点和固相/侧流测定的简单性的分析物的敏感检测技术。 可以使用磁性微球作为固体支持物在溶液/悬浮相中进行测定。 随后，可以进行磁性分离以将结合的分析物与溶液的其余部分分离。 洗涤步骤后，荧光信号可直接从磁性颗粒表面读取。 还描述了采用荧光聚合物超强化的便携式生物检测系统和用于检测其生物代谢的方法。

公开(公告)号：WO2004046687A3

公开(公告)日：2005-07-21

申请号：PCT/US0336153

申请日：2003-11-14

Applicant: QTL BIOSYSTEMS LLC ,  WHITTEN DAVID G ,  JONES ROBERT M ,  KUSHON STUART A ,  LEY KEVIN D ,  XIA WENSHENG ,  KUMARASWAMY SRIRAM ,  MCBRANCH DUNCAN W

Inventor： WHITTEN DAVID G ,  JONES ROBERT M ,  KUSHON STUART A ,  LEY KEVIN D ,  XIA WENSHENG ,  KUMARASWAMY SRIRAM ,  MCBRANCH DUNCAN W

IPC: B05D3/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N20060101 ,  G01N33/53 ,  G01N33/533 ,  G01N33/542 ,  G01N33/543 ,  G01N33/566 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/533

Abstract: Complexes of a biotinylated fluorescent polymer and a biotin binding protein and solid supports coated with the fluorescent polymer complexes are described. The complexes can be used as sensors for detecting biological recognition events (e.g., nucleic acid hybridization reactions or enzymatic induced polypeptide cleavage). Methods of making the complexes and methods of using the complexes for detecting the presence and/or amount of a target analyte in a sample are also described. The target analyte can be an enzyme (e.g., beta-secretase) or a nucleic acid (e.g., a single stranded or double stranded nucleic acid).

Abstract translation: 描述了生物素化荧光聚合物和生物素结合蛋白的复合物和涂覆有荧光聚合物复合物的固体载体。 复合物可以用作检测生物识别事件（例如，核酸杂交反应或酶诱导的多肽切割）的传感器。 还描述了制备复合物的方法和使用复合物检测样品中目标分析物的存在和/或量的方法。 靶分析物可以是酶（例如β-分泌酶）或核酸（例如，单链或双链核酸）。

公开(公告)号：WO2017083252A1

公开(公告)日：2017-05-18

申请号：PCT/US2016/060903

申请日：2016-11-08

Applicant: NEOTERYX, LLC ,  KUSHON, Stuart, A. ,  ZAMBA, Gene ,  RUDGE, James, Byron ,  JARING, Carolyn

Inventor： KUSHON, Stuart, A. ,  ZAMBA, Gene ,  RUDGE, James, Byron ,  JARING, Carolyn

IPC: A61B5/15 ,  B01D61/38 ,  G01N33/48

CPC classification number: B01L3/5023 ,  A61B5/150022 ,  A61B5/150061 ,  A61B5/150099 ,  A61B5/150221 ,  A61B5/150236 ,  A61B5/150244 ,  A61B5/150343 ,  A61B5/150755 ,  B01D15/10 ,  B01D15/26 ,  B01L3/502 ,  B01L3/5029 ,  B01L2200/0605 ,  B01L2200/0631 ,  B01L2200/0678 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/10 ,  B01L2400/0406 ,  B01L2400/049 ,  G01N1/34 ,  G01N33/491

Abstract: The present invention discloses a plasma extraction device for generating fixed, predetermined quantity of plasma and for dry-transport of obtained plasma for automated assay. Plasma extraction device includes a plasma extractor assembly comprising an absorbent probe that wicks a predetermined volume of a liquid sample from a liquid source, a separator that generates plasma from the wicked liquid sample, and an absorbent reservoir that stores fixed, predetermined quantity of the generated plasma for dry-transport and automated assay thereof.

Abstract translation: 本发明公开了一种等离子体提取装置，用于产生固定的预定量的等离子体并用于干运输所获得的等离子体以进行自动化测定。 等离子体提取装置包括等离子体提取器组件，该等离子体提取器组件包括从液体源吸取预定体积的液体样品的吸收性探头，从该被吸收的液体样品产生等离子体的分离器以及储存固定的预定量的所产生的 用于干运输的血浆和自动化测定。

公开(公告)号：WO2004046687A2

公开(公告)日：2004-06-03

申请号：PCT/US2003/036153

申请日：2003-11-14

Applicant: QTL BIOSYSTEMS, LLC ,  WHITTEN, David, G. ,  JONES, Robert, M. ,  KUSHON, Stuart, A. ,  LEY, Kevin, D. ,  XIA, Wensheng ,  KUMARASWAMY, Sriram ,  McBRANCH, Duncan, W.

Inventor： WHITTEN, David, G. ,  JONES, Robert, M. ,  KUSHON, Stuart, A. ,  LEY, Kevin, D. ,  XIA, Wensheng ,  KUMARASWAMY, Sriram ,  McBRANCH, Duncan, W.

IPC: G01N

CPC classification number: G01N33/533

Abstract: Complexes of a biotinylated fluorescent polymer and a biotin binding protein and solid supports coated with the fluorescent polymer complexes are described. The complexes can be used as sensors for detecting biological recognition events (e.g., nucleic acid hybridization reactions or enzymatic induced polypeptide cleavage). Methods of making the complexes and methods of using the complexes for detecting the presence and/or amount of a target analyte in a sample are also described. The target analyte can be an enzyme (e.g., β-secretase) or a nucleic acid (e.g., a single stranded or double stranded nucleic acid).

Abstract translation: 描述了生物素化的荧光聚合物和生物素结合蛋白的复合物和涂覆有荧光聚合物复合物的固体载体。 复合物可用作检测生物识别事件（例如，核酸杂交反应或酶诱导的多肽切割）的传感器。 还描述了制备复合物的方法和使用复合物检测样品中目标分析物的存在和/或量的方法。 靶分析物可以是酶（例如，β-分泌酶）或核酸（例如单链或双链核酸）。